The document provides an overview of products and services from Miltenyi Biotec for various applications involving mesenchymal stem cells (MSCs), including phenotyping, molecular analysis, differentiation, characterization, isolation, expansion, and translation to clinical applications of both human and mouse MSCs. It lists specific kits, media, cytokines, and equipment for sample preparation, cell separation, analysis, culture, and molecular and clinical applications.
O documento discute:
1) A origem e evolução da não-cumulatividade do IVA em diferentes países desde 1918.
2) A definição de insumo como bens e serviços consumidos no processo produtivo ou na prestação de serviços.
3) Exemplos de despesas que são e não são consideradas insumos para efeito de crédito tributário.
The document summarizes the student's visits to several institutions at the University of the Philippines Los Baños including the Museum of Natural History, Institute of Plant Breeding, College of Veterinary Medicine, Dairy Training and Research Institute, and International Rice Research Institute. At each stop, the students learned about various topics related to natural history, agriculture, veterinary science, dairy production, and rice research through exhibits, videos, and tours.
Stem cell enumeration involves quantifying stem cells, typically through CD34+ cell counting. Key methods include mononuclear cell counts, colony forming unit assays, and CD34+ enumeration via flow cytometry. For flow cytometry, the ISHAGE protocol is commonly used and involves sequential gating of lymphocytes, CD34+ cells, and viable cells. Accurate stem cell quantification is important for determining transplant timing and adequacy.
La présentation sur Nancytomique faite lors de la réunion scientifique sur le Laboratoire sans murs organisée avec le consulat de France dans le cadre de la Filière Médicale Francophone Nancy-Wuhan à la Faculté de Médecine de Wuhan et à l'Hôpital Zhongnan.
O documento discute:
1) A origem e evolução da não-cumulatividade do IVA em diferentes países desde 1918.
2) A definição de insumo como bens e serviços consumidos no processo produtivo ou na prestação de serviços.
3) Exemplos de despesas que são e não são consideradas insumos para efeito de crédito tributário.
The document summarizes the student's visits to several institutions at the University of the Philippines Los Baños including the Museum of Natural History, Institute of Plant Breeding, College of Veterinary Medicine, Dairy Training and Research Institute, and International Rice Research Institute. At each stop, the students learned about various topics related to natural history, agriculture, veterinary science, dairy production, and rice research through exhibits, videos, and tours.
Stem cell enumeration involves quantifying stem cells, typically through CD34+ cell counting. Key methods include mononuclear cell counts, colony forming unit assays, and CD34+ enumeration via flow cytometry. For flow cytometry, the ISHAGE protocol is commonly used and involves sequential gating of lymphocytes, CD34+ cells, and viable cells. Accurate stem cell quantification is important for determining transplant timing and adequacy.
La présentation sur Nancytomique faite lors de la réunion scientifique sur le Laboratoire sans murs organisée avec le consulat de France dans le cadre de la Filière Médicale Francophone Nancy-Wuhan à la Faculté de Médecine de Wuhan et à l'Hôpital Zhongnan.
The document discusses various topics related to molecular profiling and personalized medicine. It describes first generation molecular profiling techniques like gene sequencing, microarrays, and PCR. It then covers next generation sequencing technologies like Roche 454, Illumina, and ABI SOLID. It also discusses second generation techniques for DNA and RNA profiling including exome sequencing, ChIP-seq, and RNA-seq. Finally, it briefly mentions third generation sequencing and epigenetic profiling.
Webinar_Exosome Isolation and Monitoring- from cell culture to clinically rel...Ketil Winther Pedersen
1. This webinar discusses methods for isolating and analyzing exosomes directly from cell culture media and urine using magnetic bead capture with Dynabeads.
2. Direct capture of exosomes with magnetic beads is recommended for monitoring exosome production during cell culture, analysis of limited sample volumes, simplified workflow, and compatibility with downstream applications like flow cytometry and western blotting.
3. Pre-enrichment methods like ultracentrifugation may be needed for samples with low exosome concentrations but yield less sample and are more complex, while direct capture is shown to work well for cell culture media and urine samples.
This document discusses next generation molecular profiling technologies. It begins with an overview of first generation molecular profiling techniques like gene sequencing, microarrays, and fluorescence in situ hybridization (FISH). It then describes some key advantages of next generation sequencing technologies like their ability to generate more sequence data at lower cost. Examples of second generation DNA and RNA profiling methods are provided, including exome sequencing and RNA-sequencing. The document also briefly discusses emerging areas like third generation sequencing and next generation protein profiling using mass spectrometry. Epigenetic profiling using techniques like methyl-binding domain sequencing is summarized in the section on next generation epigenetic profiling.
Epiontis immune monitoring and companion diagnostics 2013TOKBSL
Epiontis introduces novel epigenetic immune cell markers that enable standardized immune monitoring from frozen whole blood or tissue samples. The markers allow precise quantification of immune cells using quantitative PCR targeting cell type-specific epigenetic modifications. The assays require small sample volumes and can measure a wide range of immune cell types from various sample types and storage conditions. Epiontis has extensive experience and quality management systems for clinical trial immune monitoring.
KCAS provides clinical flow cytometry services to support biologics and small molecule development. Their experienced scientists offer custom assay development and validation as well as sample processing and consulting for a variety of applications including pharmacodynamic assays, live cell assays, PBMC isolation, mechanism of action studies, and biomarker assays. KCAS has experience measuring immune cell subsets such as T cells, B cells, macrophages, and dendritic cells to monitor biologics and the immune system for therapeutic areas including allergy and asthma, autoimmune disease, infectious disease, oncology, stem cell enumeration, transplantation, and vaccines.
Microarrays are tools used to analyze gene expression. They consist of small glass slides containing samples of many genes arranged in a regular pattern. The genes are used to detect mRNA species present in a cell or tissue at a given time, allowing analysis of which genes are actively being expressed. Major uses of microarrays include analysis of gene expression, single nucleotide polymorphisms, and identification of pathogens or tumors. They provide a way to detect differences in gene expression across large numbers of genes in different cell populations on a genome-wide scale.
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
The document provides an overview of genomics and molecular profiling techniques. It discusses:
- The lab for bioinformatics and computational genomics which has 10 "genome hackers" and 42 scientists.
- An introduction to personalized medicine and biomarkers.
- First generation molecular profiling techniques like gene sequencing, microarrays, PCR.
- Next generation sequencing techniques like Roche 454, Illumina, SOLID which allow high throughput sequencing.
- Next generation applications like RNA sequencing, exome sequencing, epigenetic profiling.
- The role of bioinformatics in analyzing large genomic and molecular profiling data.
High Through-Put DNA Methylation Analysis of Lung Cancer: Plasma cfDNA for Bi...Kate Barlow
• Technology pipeline for methylation biomarker
development
• High throughput DNA methylation-qPCR workflows
• Liquid biopsy – cfDNA methylation testing
The document outlines 5 training modules that provide instruction on various molecular biology and microbiology techniques, including PCR, gene expression studies, and nucleic acid extraction. It also lists specialized modules on molecular cloning, proteomics, cell culture, cancer functional studies, and DNA damage assays. The cost for each individual module ranges from Rs. 6,000 to Rs. 30,000, with discounts provided for bundled modules. Special fee offers combine modules for prices between Rs. 55,000 and Rs. 1,15,000.
This document provides an overview of the genomics, proteomics, and bioinformatics services offered by CPGR (Complete Genomics & Proteomics on the African continent). It details the sample preparation, platforms, workflows, and outputs for a range of omics technologies including microarrays, next-generation sequencing, mass spectrometry, protein arrays, and bioinformatics. Standardized protocols are available for nucleic acid and protein isolation from various sample types. Bioinformatics analysis is supported for experimental design, gene/protein expression, copy number variation, SNP genotyping, pathway analysis, and more.
Single-cell technologies — studying rheumatic diseases one cell.pptxRitasman Baisya
Single-cell technologies allow studying diseases like rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) at the single-cell level. This document discusses several single-cell analysis methods including single-cell RNA sequencing to study gene expression, mass cytometry to study protein markers, and paired antigen receptor sequencing to study B and T cell receptors. These techniques are revealing new cell subpopulations and pathways involved in RA and SLE pathogenesis. When applied to tissues like synovium, they provide insights into disease mechanisms and new therapeutic targets.
Single-cell technologies — studying rheumatic diseases one cell.pptxRitasman Baisya
Single-cell technologies allow studying diseases like rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) at the single-cell level. This document discusses several single-cell omics techniques including single-cell RNA sequencing, proteomics using mass cytometry, and mass spectrometry imaging. These techniques help characterize cell heterogeneity, identify rare cell populations, and better understand pathogenic mechanisms in rheumatic diseases. The document provides examples of how these techniques have provided insights into RA, SLE, and other rheumatic conditions by analyzing cells from tissues like synovium at the single-cell level.
Utilization of NGS to Identify Clinically-Relevant Mutations in cfDNA: Meet t...QIAGEN
Pancreatic cancer is a uniquely lethal malignancy characterized by frequent mutations in KRAS, CDKN2A, SMAD4, TP53 and many others. We have shown that KRAS mutation can be detected in cell-free, circulating tumor DNA (ctDNA) isolated from the plasma in a subset of patients and is associated with poor prognosis. The ability to simultaneously detect multiple pancreatic cancer-specific mutations in ctDNA would open a new avenue for detection of clinically-relevant mutations. In this study, we performed ultra-deep sequencing of ctDNA from advanced pancreatic cancer patients prior to treatment with Gemcitabine and Erlotinib following target enrichment. Somatic, non-synonymous variants were identified in 29 different genes at allele frequencies typically less than 0.5%. Updated results of ultra-deep NGS analysis will be presented.
This document summarizes a study that aimed to identify prognostic serum microRNA profiles in neuroblastoma. The study performed microRNA profiling on serum samples from different neuroblastoma patient risk groups. Ten microRNAs were found to best discriminate between survival groups. A validation set of over 120 patients was then profiled for these 10 microRNAs, finding they could separate high-risk deceased from low-risk survivor patients. This suggests serum microRNA profiling may help identify ultra-high risk neuroblastoma patients. The document also discusses microRNA biomarker potential in general and optimization of microRNA sequencing workflows from serum samples.
CRC provides central laboratory services for clinical trials including maintaining confidentiality and regulatory standards. It has over 10 years experience working with pharmaceutical companies on preclinical and clinical trials. CRC offers a wide range of in vitro and in vivo ADME, pharmacokinetic, toxicology and safety pharmacology studies. It also provides molecular biology, analytical chemistry and other specialized testing services to support drug discovery and development.
Specialty pathology endpoints for all clients, including complex immunohistochemistry, in situ hybridization, image analysis, artificial intelligence, stereology, tissue cross-reactivity, and electron microscopy.
This document discusses immunohistochemistry and provides information on various markers used in pathology. It is divided into four broad categories of staining patterns and discusses markers for epithelial, mesenchymal, lymphoid, neuroendocrine, oncofetal and other types of differentiation. Specific markers are outlined for tissues, tumors and infectious organisms. Immunohistochemistry is useful for tumor diagnosis, assessing prognosis, and identifying predictive markers for targeted therapies.
MOLECULAR AND CYTOGENETIC ANALYSIS -BMLS GENERAL &HBT-1.pptxAmosiRichard
Molecular and cytogenetic analysis are essential techniques for diagnosing and managing hematological disorders. Key methods include DNA extraction, PCR, FISH, and next generation sequencing. Clinical applications involve investigating diseases like sickle cell anemia, thalassemias, leukemias, lymphomas, and coagulation disorders. Molecular analysis allows identification of genetic mutations and translocations that underlie these conditions and guides treatment decisions. While providing critical diagnostic information, these techniques also have limitations like risk of infection and interference from therapies.
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...QIAGEN
The original microRNA quality control (miRQC) study provided an in-depth analysis of commercially available microRNA (miRNA) quantification platforms. Specifically, twelve different
microarray, real-time PCR and small RNA sequencing platforms were assessed for reproducibility, sensitivity, accuracy, specificity and concordance of differential expression using a variety of sample types. Overall, each platform exhibited specific strengths and weaknesses, leading to the
final suggestion that a platform should be chosen on the basis of the experimental setting and the specific research questions. With this suggestion in mind, and the fact that liquid miRNA biopsies are an area of intense interest, we sought to expand the original miRQC study. For our “miRQC extension,” we benchmarked the QIAGEN miScript® PCR System with and without preamplification, and included a specific focus on routinely used biofluids. Concurrently, we benchmarked the miScript PCR System against another SYBR® Green miRNA detection platform. Overall, QIAGEN miScript demonstrated strong reproducibility and accuracy as well as superior detection rate and sensitivity in biofluids. Collectively, QIAGEN miScript provides the leading solution for novel miRNA discoveries.
This document discusses the history and development of Miltenyi Biotec over the past 25 years. It describes how Miltenyi Biotec revolutionized cell processing through the development of MACS Technology, which allows for gentle and rapid magnetic separation of cells. Key developments include the CliniMACS System for clinical-scale cell isolation, and the recent FDA approval of the CliniMACS CD34 Reagent System for graft-versus-host disease prophylaxis. The document also introduces the CliniMACS Prodigy which further automates complex cell manufacturing workflows in a closed system.
This newsletter article describes using the gentleMACS Dissociator to generate tumor-infiltrating lymphocyte (TIL) cultures from melanoma tumors for adoptive cell therapy. The gentleMACS Dissociator mechanically dissociates tumor tissue in a closed, automated system, providing a simpler and more standardized alternative to traditional enzyme-based dissociation methods. Experiments optimized variables like program settings, enzymes, incubation times and sample size. The results identified a standard operating procedure using the gentleMACS Dissociator that yields high cell yields and viability in a reproducible manner for generating TIL cultures from clinical tumor samples.
The document discusses various topics related to molecular profiling and personalized medicine. It describes first generation molecular profiling techniques like gene sequencing, microarrays, and PCR. It then covers next generation sequencing technologies like Roche 454, Illumina, and ABI SOLID. It also discusses second generation techniques for DNA and RNA profiling including exome sequencing, ChIP-seq, and RNA-seq. Finally, it briefly mentions third generation sequencing and epigenetic profiling.
Webinar_Exosome Isolation and Monitoring- from cell culture to clinically rel...Ketil Winther Pedersen
1. This webinar discusses methods for isolating and analyzing exosomes directly from cell culture media and urine using magnetic bead capture with Dynabeads.
2. Direct capture of exosomes with magnetic beads is recommended for monitoring exosome production during cell culture, analysis of limited sample volumes, simplified workflow, and compatibility with downstream applications like flow cytometry and western blotting.
3. Pre-enrichment methods like ultracentrifugation may be needed for samples with low exosome concentrations but yield less sample and are more complex, while direct capture is shown to work well for cell culture media and urine samples.
This document discusses next generation molecular profiling technologies. It begins with an overview of first generation molecular profiling techniques like gene sequencing, microarrays, and fluorescence in situ hybridization (FISH). It then describes some key advantages of next generation sequencing technologies like their ability to generate more sequence data at lower cost. Examples of second generation DNA and RNA profiling methods are provided, including exome sequencing and RNA-sequencing. The document also briefly discusses emerging areas like third generation sequencing and next generation protein profiling using mass spectrometry. Epigenetic profiling using techniques like methyl-binding domain sequencing is summarized in the section on next generation epigenetic profiling.
Epiontis immune monitoring and companion diagnostics 2013TOKBSL
Epiontis introduces novel epigenetic immune cell markers that enable standardized immune monitoring from frozen whole blood or tissue samples. The markers allow precise quantification of immune cells using quantitative PCR targeting cell type-specific epigenetic modifications. The assays require small sample volumes and can measure a wide range of immune cell types from various sample types and storage conditions. Epiontis has extensive experience and quality management systems for clinical trial immune monitoring.
KCAS provides clinical flow cytometry services to support biologics and small molecule development. Their experienced scientists offer custom assay development and validation as well as sample processing and consulting for a variety of applications including pharmacodynamic assays, live cell assays, PBMC isolation, mechanism of action studies, and biomarker assays. KCAS has experience measuring immune cell subsets such as T cells, B cells, macrophages, and dendritic cells to monitor biologics and the immune system for therapeutic areas including allergy and asthma, autoimmune disease, infectious disease, oncology, stem cell enumeration, transplantation, and vaccines.
Microarrays are tools used to analyze gene expression. They consist of small glass slides containing samples of many genes arranged in a regular pattern. The genes are used to detect mRNA species present in a cell or tissue at a given time, allowing analysis of which genes are actively being expressed. Major uses of microarrays include analysis of gene expression, single nucleotide polymorphisms, and identification of pathogens or tumors. They provide a way to detect differences in gene expression across large numbers of genes in different cell populations on a genome-wide scale.
Development of quality control assays for cell-based medicinal products (ISCT...Quality Assistance s.a.
Dr. Fabian Vandermeers from Quality Assistance spoke on Development of quality control assays for cell-based medicinal products at ISCT 2017 in London.
For more information on this topic, visit: http://www.quality-assistance.com/analytical-services/CBMPs
For more information on our expertise and services, visit: www.quality-assistance.com
Follow us on social media:
LinkedIn: https://www.linkedin.com/company/quality-assistance
Twitter: https://twitter.com/QA_Belgium
Facebook: https://www.facebook.com/QualityAssistanceBelgium
Google +: https://plus.google.com/103676189647965359292
Quality Assistance S.A. is a leading European Contract Research Organisation providing the pharmaceutical industry with all the analytical services required by EMA and FDA regulations for the development and marketing of innovative human medicinal products.
We assist our clients from candidate selection, through non-clinical and clinical studies, to marketing authorisation, using our state-of-the-art, product-dedicated expertise in analytical sciences.
For each customer and each project, we design customised solutions, define analytical protocols, develop and validate specific new analytical methods and perform characterisation, stability, pharmacokinetic, biomarker and immunogenicity studies as well as batch release testing, in order to evaluate the Quality, Safety and Efficacy of the given drugs.
The document provides an overview of genomics and molecular profiling techniques. It discusses:
- The lab for bioinformatics and computational genomics which has 10 "genome hackers" and 42 scientists.
- An introduction to personalized medicine and biomarkers.
- First generation molecular profiling techniques like gene sequencing, microarrays, PCR.
- Next generation sequencing techniques like Roche 454, Illumina, SOLID which allow high throughput sequencing.
- Next generation applications like RNA sequencing, exome sequencing, epigenetic profiling.
- The role of bioinformatics in analyzing large genomic and molecular profiling data.
High Through-Put DNA Methylation Analysis of Lung Cancer: Plasma cfDNA for Bi...Kate Barlow
• Technology pipeline for methylation biomarker
development
• High throughput DNA methylation-qPCR workflows
• Liquid biopsy – cfDNA methylation testing
The document outlines 5 training modules that provide instruction on various molecular biology and microbiology techniques, including PCR, gene expression studies, and nucleic acid extraction. It also lists specialized modules on molecular cloning, proteomics, cell culture, cancer functional studies, and DNA damage assays. The cost for each individual module ranges from Rs. 6,000 to Rs. 30,000, with discounts provided for bundled modules. Special fee offers combine modules for prices between Rs. 55,000 and Rs. 1,15,000.
This document provides an overview of the genomics, proteomics, and bioinformatics services offered by CPGR (Complete Genomics & Proteomics on the African continent). It details the sample preparation, platforms, workflows, and outputs for a range of omics technologies including microarrays, next-generation sequencing, mass spectrometry, protein arrays, and bioinformatics. Standardized protocols are available for nucleic acid and protein isolation from various sample types. Bioinformatics analysis is supported for experimental design, gene/protein expression, copy number variation, SNP genotyping, pathway analysis, and more.
Single-cell technologies — studying rheumatic diseases one cell.pptxRitasman Baisya
Single-cell technologies allow studying diseases like rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) at the single-cell level. This document discusses several single-cell analysis methods including single-cell RNA sequencing to study gene expression, mass cytometry to study protein markers, and paired antigen receptor sequencing to study B and T cell receptors. These techniques are revealing new cell subpopulations and pathways involved in RA and SLE pathogenesis. When applied to tissues like synovium, they provide insights into disease mechanisms and new therapeutic targets.
Single-cell technologies — studying rheumatic diseases one cell.pptxRitasman Baisya
Single-cell technologies allow studying diseases like rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE) at the single-cell level. This document discusses several single-cell omics techniques including single-cell RNA sequencing, proteomics using mass cytometry, and mass spectrometry imaging. These techniques help characterize cell heterogeneity, identify rare cell populations, and better understand pathogenic mechanisms in rheumatic diseases. The document provides examples of how these techniques have provided insights into RA, SLE, and other rheumatic conditions by analyzing cells from tissues like synovium at the single-cell level.
Utilization of NGS to Identify Clinically-Relevant Mutations in cfDNA: Meet t...QIAGEN
Pancreatic cancer is a uniquely lethal malignancy characterized by frequent mutations in KRAS, CDKN2A, SMAD4, TP53 and many others. We have shown that KRAS mutation can be detected in cell-free, circulating tumor DNA (ctDNA) isolated from the plasma in a subset of patients and is associated with poor prognosis. The ability to simultaneously detect multiple pancreatic cancer-specific mutations in ctDNA would open a new avenue for detection of clinically-relevant mutations. In this study, we performed ultra-deep sequencing of ctDNA from advanced pancreatic cancer patients prior to treatment with Gemcitabine and Erlotinib following target enrichment. Somatic, non-synonymous variants were identified in 29 different genes at allele frequencies typically less than 0.5%. Updated results of ultra-deep NGS analysis will be presented.
This document summarizes a study that aimed to identify prognostic serum microRNA profiles in neuroblastoma. The study performed microRNA profiling on serum samples from different neuroblastoma patient risk groups. Ten microRNAs were found to best discriminate between survival groups. A validation set of over 120 patients was then profiled for these 10 microRNAs, finding they could separate high-risk deceased from low-risk survivor patients. This suggests serum microRNA profiling may help identify ultra-high risk neuroblastoma patients. The document also discusses microRNA biomarker potential in general and optimization of microRNA sequencing workflows from serum samples.
CRC provides central laboratory services for clinical trials including maintaining confidentiality and regulatory standards. It has over 10 years experience working with pharmaceutical companies on preclinical and clinical trials. CRC offers a wide range of in vitro and in vivo ADME, pharmacokinetic, toxicology and safety pharmacology studies. It also provides molecular biology, analytical chemistry and other specialized testing services to support drug discovery and development.
Specialty pathology endpoints for all clients, including complex immunohistochemistry, in situ hybridization, image analysis, artificial intelligence, stereology, tissue cross-reactivity, and electron microscopy.
This document discusses immunohistochemistry and provides information on various markers used in pathology. It is divided into four broad categories of staining patterns and discusses markers for epithelial, mesenchymal, lymphoid, neuroendocrine, oncofetal and other types of differentiation. Specific markers are outlined for tissues, tumors and infectious organisms. Immunohistochemistry is useful for tumor diagnosis, assessing prognosis, and identifying predictive markers for targeted therapies.
MOLECULAR AND CYTOGENETIC ANALYSIS -BMLS GENERAL &HBT-1.pptxAmosiRichard
Molecular and cytogenetic analysis are essential techniques for diagnosing and managing hematological disorders. Key methods include DNA extraction, PCR, FISH, and next generation sequencing. Clinical applications involve investigating diseases like sickle cell anemia, thalassemias, leukemias, lymphomas, and coagulation disorders. Molecular analysis allows identification of genetic mutations and translocations that underlie these conditions and guides treatment decisions. While providing critical diagnostic information, these techniques also have limitations like risk of infection and interference from therapies.
Extending miRQC’s dynamic range: amplifying the view of Limiting RNA samples ...QIAGEN
The original microRNA quality control (miRQC) study provided an in-depth analysis of commercially available microRNA (miRNA) quantification platforms. Specifically, twelve different
microarray, real-time PCR and small RNA sequencing platforms were assessed for reproducibility, sensitivity, accuracy, specificity and concordance of differential expression using a variety of sample types. Overall, each platform exhibited specific strengths and weaknesses, leading to the
final suggestion that a platform should be chosen on the basis of the experimental setting and the specific research questions. With this suggestion in mind, and the fact that liquid miRNA biopsies are an area of intense interest, we sought to expand the original miRQC study. For our “miRQC extension,” we benchmarked the QIAGEN miScript® PCR System with and without preamplification, and included a specific focus on routinely used biofluids. Concurrently, we benchmarked the miScript PCR System against another SYBR® Green miRNA detection platform. Overall, QIAGEN miScript demonstrated strong reproducibility and accuracy as well as superior detection rate and sensitivity in biofluids. Collectively, QIAGEN miScript provides the leading solution for novel miRNA discoveries.
This document discusses the history and development of Miltenyi Biotec over the past 25 years. It describes how Miltenyi Biotec revolutionized cell processing through the development of MACS Technology, which allows for gentle and rapid magnetic separation of cells. Key developments include the CliniMACS System for clinical-scale cell isolation, and the recent FDA approval of the CliniMACS CD34 Reagent System for graft-versus-host disease prophylaxis. The document also introduces the CliniMACS Prodigy which further automates complex cell manufacturing workflows in a closed system.
This newsletter article describes using the gentleMACS Dissociator to generate tumor-infiltrating lymphocyte (TIL) cultures from melanoma tumors for adoptive cell therapy. The gentleMACS Dissociator mechanically dissociates tumor tissue in a closed, automated system, providing a simpler and more standardized alternative to traditional enzyme-based dissociation methods. Experiments optimized variables like program settings, enzymes, incubation times and sample size. The results identified a standard operating procedure using the gentleMACS Dissociator that yields high cell yields and viability in a reproducible manner for generating TIL cultures from clinical tumor samples.
This poster summarizes results presented at the 2010 American Society of Blood and Marrow Transplantation meeting comparing outcomes of two stem cell transplantation trials for acute myeloid leukemia patients. The trials utilized either T-cell depleted grafts (TCD) after CD34+ selection or unmodified grafts. While disease-free survival, overall survival, and transplant-related mortality were similar between the groups, the TCD group had significantly lower rates of acute graft-versus-host disease (20% vs 38%) and chronic GVHD (19% vs 47%) without increased relapse risk. The results support T-cell depletion in matched transplantation and suggest its extension to matched unrelated transplantation.
The document provides an overview of products and services from Miltenyi Biotec for various stages of mesenchymal stem cell (MSC) research, including phenotyping, molecular analysis, differentiation, characterization, isolation, expansion, and translation to clinical applications for both human and mouse MSCs. It lists specific kits, media, cytokines, and equipment for sample preparation, cell separation, analysis, culture, and molecular applications.
The document describes the BD FACSJazz cell sorter. It is a compact, affordable benchtop cell sorter that incorporates design features to simplify operation for researchers with limited flow cytometry experience. Key features include factory-optimized settings, intuitive alignment, real-time video monitoring, and BD FACS Accudrop technology. The BD FACSJazz requires less than 2 square feet of bench space and can be configured with up to three lasers and eight parameters to support a variety of applications. It aims to make commonly used cell sorting applications more accessible to researchers.
The document discusses techniques for isolating and analyzing rare cancer cell populations using MACS Technology from Miltenyi Biotec. It describes methods for positive selection using magnetic labeling to isolate cells of interest, depletion to remove unwanted cells, and sequential sorting using both approaches. MACS MicroBeads are described as superparamagnetic particles used for magnetic labeling of cells during these separation techniques.
This document discusses Miltenyi Biotec's stem cell research products and technologies. It describes their portfolio of products for sample preparation, cell separation, cell analysis, cell culture, and molecular applications to support stem cell workflows from basic research to clinical applications. Specific cell types discussed include embryonic stem cells, induced pluripotent stem cells, hematopoietic stem cells, mesenchymal stem cells, and cancer stem cells. MACS technology for magnetic cell separation is highlighted as the gold standard and key to their product offerings.
This document appears to be an informational brochure about neuroscience research products from Miltenyi Biotec. It discusses their tissue dissociation kits that allow for fast and standardized dissociation of neural tissues. It also discusses their cell separation technology and antibodies for isolating pure populations of cells such as astrocytes, microglia, neurons and oligodendrocytes. Finally, it mentions contrast agents and services for neuroimaging and molecular analysis of isolated cells.
This document provides an overview of MACS Products that can be used for tissue regeneration research. It describes how stem and progenitor cells have potential to revolutionize tissue regeneration. MACS Products provide tools to reliably isolate defined stem cell populations with excellent purity for reproducible results. This saves time that can be used to focus on research. Isolated cells remain viable and functional for immediate use in cell culture and animal experiments. MACS Technology offers integrated solutions for sample preparation, cell separation, analysis, culture, and molecular analysis.
- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
Does Over-Masturbation Contribute to Chronic Prostatitis.pptxwalterHu5
In some case, your chronic prostatitis may be related to over-masturbation. Generally, natural medicine Diuretic and Anti-inflammatory Pill can help mee get a cure.
DECLARATION OF HELSINKI - History and principlesanaghabharat01
This SlideShare presentation provides a comprehensive overview of the Declaration of Helsinki, a foundational document outlining ethical guidelines for conducting medical research involving human subjects.
Cell Therapy Expansion and Challenges in Autoimmune DiseaseHealth Advances
There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
In addition to infrastructure and capacity constraints, CAR-Ts face a very different risk-benefit dynamic in autoimmune compared to oncology, highlighting the need for tolerable therapies with low adverse event risk. CAR-NK and Treg-based therapies are also being developed in certain autoimmune disorders and may demonstrate favorable safety profiles. Several novel non-cell therapies such as bispecific antibodies, nanobodies, and RNAi drugs, may also offer future alternative competitive solutions with variable value propositions.
Widespread adoption of cell therapies will not only require strong efficacy and safety data, but also adapted pricing and access strategies. At oncology-based price points, CAR-Ts are unlikely to achieve broad market access in autoimmune disorders, with eligible patient populations that are potentially orders of magnitude greater than the number of currently addressable cancer patients. Developers have made strides towards reducing cell therapy COGS while improving manufacturing efficiency, but payors will inevitably restrict access until more sustainable pricing is achieved.
Despite these headwinds, industry leaders and investors remain confident that cell therapies are poised to address significant unmet need in patients suffering from autoimmune disorders. However, the extent of this impact on the treatment landscape remains to be seen, as the industry rapidly approaches an inflection point.
Travel vaccination in Manchester offers comprehensive immunization services for individuals planning international trips. Expert healthcare providers administer vaccines tailored to your destination, ensuring you stay protected against various diseases. Conveniently located clinics and flexible appointment options make it easy to get the necessary shots before your journey. Stay healthy and travel with confidence by getting vaccinated in Manchester. Visit us: www.nxhealthcare.co.uk
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