Neuroscience research brochure


Published on

Published in: Health & Medicine
  • Be the first to comment

  • Be the first to like this

No Downloads
Total views
On SlideShare
From Embeds
Number of Embeds
Embeds 0
No embeds

No notes for slide

Neuroscience research brochure

  1. 1. NeuroscienceInspiring technology for creative scientists Fast, reliable tissue dissociation Simple, effective myelin removal Primary neural cell isolation in as little as one hour Contrast agents specifically optimized for small animal imaging
  2. 2. Contents Introducing a new milestone in cell analysis The MACSQuant™ Analyzer paves the way to successful research 3 Sample preparation • Time-saving and standardized dissociation of neural tissues • Removal of myelin debris for better results from adult tissue samples 6 Cell separation • MACS® Technology, the gold standard in cell separation • Pure astrocytes, microglia, neurons and oligodendrocytes • Standardization with automated cell separation 9 Cell analysis • Titrated high-quality antibodies and brilliant fluorochromes • Easy-to-use bench-top flow cytometers 10 Cell culture • Serum-free medium and supplement for long-term viability • Premium, GMP, and research grade cytokines 12 Neuroimaging • Contrast agents optimized for small animal imaging • MRI, optical imaging, CT and ultrasound 14 Molecular analysis • Fast isolation of functional mitochondria • Efficient mRNA amplification from small samples • Genomic Services for mRNA and microRNA expression2
  3. 3. Informational brochureSample preparationGet a good startTissue dissociation kits Antigen compatibility ofThe secret of success in any experiment lies in the preparation Neural Tissue Dissociation Kitsof the starting material. Get going fast with Miltenyi Biotec’s Table 1 shows the recommended kit regarding antigenNeural Tissue Dissociation Kits (NTDK) and the gentleMACS® compatibility for subsequent cell separation or analysis.Dissociators that help streamline and standardize the Epitope sensitivities were tested with MACS® Antibodies.generation of single-cell suspensions.• Gentle and efficient: titrated enzymes and optimized buffers Antigen Species Cell type• Conserved epitopes for optimal downstream applications Neural Tissue Dissociation Kits (P)• Convenience: everything in one box, including detailed protocols Prominin-1 Mouse Neural stem and progenitor cells• Reproducible results from standardized components A2B5 Human, mouse, rat Glial-restricted precursors O4 Human, mouse, rat Immature oligodendrocytesIn order to conserve the epitopes of interest, it is importantto choose the correct protease because some epitopes are CD11b Human, mouse Microgliadegraded by trypsin, others by papain. Two kits were therefore CD81¹ Mouse, rat Microglia, macrophages,developed, NTDK (T) and NDTK (P). endothelial cells, glia CD31¹ Mouse Endothelial cells CD133 Human Neural progenitor cells AN2 Mouse NG2 glia NTDK (T) NTDK (P) (mouse NG2)² 1000 1000 20% 6% Neural Tissue Dissociation Kits (T) 750 750 Prominin-1² Mouse Neural stem and progenitor 500 500 cells O4 Human, mouse, rat Immature oligodendrocytes 250 250 PSA-NCAM Human, mouse, rat Neuronal precursors, 0 0 oligodendrocyte progenitors -1 0 1 10¹ 10² 10³ -1 0 1 10¹ 10² 10³ CD24 Mouse Neuronal precursors, ependymal cells GLAST A2B5³ Human, mouse, rat Glial-restricted precursors CD11b Human, mouse MicrogliaFigure 1: Flow cytometric analysis of cells dissociated with trypsin-based NTDK (T) and papain-based NTDK (P) and then labeled with CD271 Human Schwann cells, motor neuronsAnti-GLAST-PE. After trypsin-based dissociation the epitope is conserved (LNGFR)and 20% of the cells are positive for GLAST whereas after papain-based CD105 Mouse Endothelial cellsdissociation the antibody binds to only 6%. Thus, the NTDK (T) is Ter-119 Mouse Erythrocytesrecommended for use with Anti-GLAST products. For detailed informationvisit and download poster 1. GLAST Human, mouse, rat Astrocytes, radial glia CD133 Human Neural progenitor cellsIf the epitope is not sensitive to proteases, we recommend AN2 Mouse NG2 gliathe papain-based kits. (mouse NG2)² 1 Slight epitope sensitivity with the use of the papain-based kit;• Neural Tissue Dissociation Kit (T)* and (P)* therefore, a further dilution of the enzyme mix is recommended.• Brain Tumor Dissociation Kit, human (T)* and (P)* (1:10; 5µL instead of 50 µL) 2 Incubation for re-expression of antigen necessary.• Neural Tissue Dissociation Kit – Postnatal Neurons (P) 3 Slight epitope sensivity with the use of the trypsin-based kit; therefore, use of the papain-based kit is recommended• Neurosphere Dissociation Kit (T) and (P) Epitope sensitivities have been tested with antibodies available from Miltenyi Biotec.• Embryoid Body Dissociation Kit, human and mouse ** Table 1: Antigen compatibility with papain (P) and trypsin (T)-based kits*These kits can be used for even more reliable results with the gentleMACS Dissociators. Protocols for manual dissociation using pipettes are also available. ** Kit can only be used with the gentleMACS Dissociators. 3
  4. 4. Sample preparation Get a good start Automated tissue dissociation The gentleMACS Dissociators not only combine reliability and reproducibility in an easy-to-use system, the resulting cells do Team the tissue dissociation kits with the gentleMACS not look any different from cells prepared manually. Dissociators for: • Ultimate reproducibility, independent of user A • A safe and sterile closed system • Push-button technology instead of pipetting • Processing multiple samples in parallel “… one of our research focuses is related to the role of brain-intrinsic immune cells in malignant brain tumors, especially in the most malignant variant, the glioblastoma … The best results were obtained by using the gentleMACS® system in combination with the Brain Tumor Dissociation Kit. “ B Michel Mittelbronn,Ph.D, Institute of Neurology Goethe-University Frankfurt, Germany Figure 3: Neuronal precursors in culture. The cells show the same morphology in culture after (A) manual dissociation using NTDK (T) and after (B) gentleMACS dissociation using NTDK (T). ßIII-Tubulin (green), PSA-NCAM (red). “Your digestion kit has not only improved our yields but Figure 2: The gentleMACS and gentleMACS Octo Dissociators shown with a tissue dissociation kit, C-Tubes, and M-Tubes. the preparation has much less debris. I wish I had used it when we initiated these studies.” The gentleMACS Dissociators provide different programs for Richard Ciavarra,PhD, Eastern Virginia Medical School, USA gentle preparation of single-cell suspensions or for homogenization. Up to two samples can be processed with the gentleMACS Dissociator and up to eight samples at a time with the gentleMACS Octo Dissociator. Visit to watch the preparation of neural tissue with the gentleMACS Dissociator.4
  5. 5. Removal of myelin debris AMyelin Removal Beads II before myelin removal• Effectively removes myelin debris from single-cell scatter suspensions Side scatter• Use with rodent brain older than 1 week and human samples Side• Improves antibody binding in downstream applications• No need for determination of cell/debris ratio or cell 4.2% numbers Forward scatter Forward scatter “Myelin removal beads allow us to reliably and quickly separate the “trash from the treasure”. B Noel Derecki from the Kipnis lab., Department of Neuroscience, after myelin removal University of Virginia, USA Side scatter Sidescatter without myelin removal with myelin removal 89.0% Anti-Sca-1-BTtiqa₃ / Anti-Biotin Anti-Sca-1-BTtiqa₃ / Anti-Biotin 4.48% 16.67% HK Secondp (g)₀₂βR₃ ow HK Secondp (g)₀₂βR₃ ow Forwardscatter Forward scatter CD11b-APC Figure 5: Removal of myelin debris from single-cell suspensions with Myelin Removal Beads. Postnatal (P22) mouse brains were dissociated using the Neural Tissue Dissociation Kit (P) and the resulting single-cell suspensions analyzed by flow cytometry either before or after Forward scatter Forward scatter treatment with Myelin Removal Beads. (A) Single-cell suspensions derived from mouse brain consist of large amounts of myelin membrane fragments Forward scatter and only 4% cells. (B) Myelin Removal Beads efficiently remove myelin debris.Figure 4: Staining of microglia from post-natal (P22) mouse brain withCD11b-APC.1×106 cells from a single-cell suspension of P22 mouse brain were stainedwith CD11b-APC without (left) and with (right) previous myelin removalusing Myelin Removal Beads. The dot plots show that in samples withprevious myelin removal, higher percentages of CD11b-positive cells arestained. Dead cells were excluded using propidium iodide. Only thepositive cells along with positive debris are displayed in side and forwardscatters. For detailed information visit download poster 2. 5
  6. 6. Cell separation The fast track to pure, viable cells Isolation of specific cell types from tissue samples or cell cultures, e.g., ES or iPS cells, is a prerequisite for exact analysis, Magnetic labeling efficient screening and optimal cell culture. Gentle to cells; minimal influence This is MACS® Technology: the gold standard in bench-top cell on downstream separation with more than 14,500 publications to prove it. This experiments renowned technology is also available for neuroscience research: • Preparation of astrocytes or microglia in 1 hour instead of two weeks by the shake-off method • Gentler procedure than flow sorting, simpler than immuno- Magnetic separation panning MACS® Column Technology provides a high-gradient • Optimal recovery and high purity N S magnetic field. • Directly on your bench, no experience required • Gentle to cells • Sterile sample handling • Thorough rinsing procedure • High recovery MicroBeads • Small, non-toxic, biodegradable Unlabeled cells are collected in • Conjugated to highly specific monoclonal antibodies the flow-through • Compatible with flow cytometry analysis MACS Columns • Amplify the magnetic field, only small amounts Elution of the labeled cell fraction of MicroBead labeling required Optimal results–even for rare • Cell-friendly steel matrix cells–by using positive selection N S • Depletion and enrichment of up to 2×1010 total cells Watch the isolation of astrocytes with Anti-GLAST (ACSA-1) MicroBeads on A B C N Figure 7: The principle of MACS Technology for depletion or enrichment of Figure 6: The features of MicroBeads and MACS Columns cells. (A) Scanning electron microscopy of a cell isolated with MACS MicroBeads (B) 50 nm MicroBeads are so small they can only be seen on a Transmission Electron Microscope (C). A cross section of a MACS Column showing the steel ball matrix (gray) with the magnetic field in which labeled cells (purple) are retained.6
  7. 7. Direct conjugates of monoclonal antibodies and MicroBeads “We are studying neuron-glia interactions using primaryare optimized and already titrated for you cultures of highly purified neurons and glial cells. Previously, we isolated cells by immunopanning. Cell type Product Species … we switched to magnetic cell isolation kits from Neurons Miltenyi Biotec because of three advantages. First, the cell preparation is more economical, second, it takes two Retinal ganglion Retinal Ganglion Cell cells Isolation Kit Rat instead of six hours, and third, it delivers very pure, healthy cells.” Neuronal Anti-PSA-NCAM-MicroBeads Human, precursors mouse, rat Frank W. Pfrieger Ph.D. Institute of Cellular and Integrative Neurons Neuron Isolation Kit Mouse Neurosciences (INCI), France Astrocytes Astrocytes and Anti-GLAST (ACSA-1) Human, radial glia MicroBead Kit mouse, rat Original Negative Positive fraction fraction fraction Astrocytes Anti-ACSA-2 MicroBeads 9.1% 0.75% 98.7% (coming soon) CD11b Oligodendrocytes Immature Anti-O4 MicroBeads Human, oligodendrocytes mouse, rat Oligodendrocyte Anti-A2B5 MicroBeads Human, precursors mouse, rat Forward scatter NG2 glia/ Anti-AN2 MicroBeads Mouse Polydendrocytes Figure 8: MACS Technology enables isolation of cells even from adult Schwann cells CD271 (LNGFR) MicroBead Kit Human tissue samples. Enrichment of human microglia from a glioblastoma Neural progenitors sample achieved a purity of 99%. For detail information visit and download poster 3. Neural progenitors CD133 MicroBead Kit Human Neural progenitors Anti-Prominin-1 MicroBeads Mouse A B Neural crest cells CD271 (LNGFR) MicroBead Kit Human ES/iPS derived Neural Crest Stem Cell Neural crest cells MicroBeads (coming soon) Microglia Microglia CD11b (Microglia) MicroBeads Human, mouse T cells T helper cells CD4 (L3T4) MicroBeads Mouse Regulatory T cells CD4+CD25+ Regulatory T Cell Mouse Figure 9: MACS Technology is optimal for effective downstream Isolation Kit, mouse applications. (A) Human brain biopsies were dissociated using the Brain Tumor Dissociation Kit (P) and the gentleMACS Dissociator. Myelin was Dendritic cells removed using Myelin Removal Beads, and cells isolated using CD11b (Microglia) MicroBeads. The microglia show a normal morphology. Dendritic cells CD11c MicroBeads, mouse Mouse (B) Astrocytes (7 DIV) isolated from P1 mice using the Anti-GLAST (ACSA-1)Table 2: Examples of cell separation reagents available for neuroscience MicroBead Kit. Astrocytes were stained using Anti-GLAST (ACSA-1) (green)research and Anti-GFAP (red). Cells were cultured in MACS® Neuro Medium and MACS® Supplement B27 PLUS, 0.5 mM L-glutamine and 1% Pen/Strep on poly-D-lysine-coated coverslips. 7
  8. 8. Cell separation The fast track to pure, viable cells Manual cell separation All that is necessary for manual separation is a MultiStand with a MACS Separator, columns and our separation reagents. The Starter Kits are the easiest way to begin cell separation: • Simple: the kit contains everything you need – separator, columns and MicroBeads • Flexible: order with antigen-specific MicroBeads of your choice • Value: save on the price of individual components Automated cell separation The autoMACS Pro® Separator is a benchtop automated Figure 10: OctoMACS Separator with MS Columns on MACS MultiStand magnetic cell sorter for the isolation of virtually any cell type from any species: • Convenient: standardized walk-away cell isolation • Versatile: isolate virtually any cell type, and also remove myelin debris • Simple: an intuitive touchscreen interface and preset programs for optimal results • Flexible: with high-speed sorting of more than 10 million cells per second and volumes from 0.2 mL to 50 mL. • Time-saving: Multiple samples with less hands-on time “In contrast to very low numbers of microglia obtained with conventional cellular depletion methods, we could increase the purity to more than 95% by using CD11b Microglia MicroBeads. Figure 11: autoMACS Pro Separator Based on our long-term experience, we can highly recommend the products from Miltenyi Biotec. These products allow a time-efficient and reproducible cell separation, also in a high-throughput manner and with an excellent quality.“ Michel Mittelbronn,Ph.D, Institute of Neurology Goethe-University Frankfurt, Germany8
  9. 9. Cell analysisAnalyze millions of cells in secondsRevolutionize neural cell analysis with a range of premiumquality antibodies, brilliant fluorochromes, and novel MACSQuant® Analyzersinstrumentation. Discover the benefits of flow cytometry. • Easy to use, best in class, flow cytometry for experts and• As an alternative to immunohistochemistry and beginners alike cytochemistry, a flow cytometer will measure millions of • 3 Lasers and up to 10 optical parameters cells in seconds and enables the analysis of cell populations • Absolute cell counting using multiple markers for a more accurate assessment of the whole cell population • Rare cell analysis• Complementing Western Blotting, a flow cytometer can • 96-well automation analyze proteins with quantitative analysis on a cell for cell • Compact bench-top design also adds value to a core facility basis, analyzing up to eight proteins at once rather than one • Live, around the clock, remote support at a timeFlow cytometry enables the exact quantification of cellpopulations and analysis of overlapping markers. Dot plotsdepict cells and smaller particles as dots (events) and illustratea staining for a marker by a shift on the respective axis. A B C 1.9% 4.8% 3.2% 4.9% 4.3% 0.4% AN2-PE 14.0% 11.6% 4.4% A2B5-APC Figure 13: The MACSQuant Analyzer family. MACSQuant Analyzer: a bench-top cell analyzer for highly sensitive multicolor flow analysis, withFigure 12: Cells from P1 (A), P3 (B) and P7 (C) mouse cortex were up to ten parameters. MACSQuant VYB: a versatile optical layout, poweredstained with Anti-AN2-PE and Anti-A2B5-APC. AN2+ cells are in the by a 561 nm laser for fluorescent proteins like GFP, YFP and mCherry.two upper quadrants, while A2B5+ cells are in the upper right and lowerright quadrants. The percentage of cells that are positive for both markersis shown in the upper right quadrant. The dot plots show a significant See for yourself how MACS Cell Analysis can be at thedecrease of A2B5 positive cells with increasing animal age, so that the heart of your experiments. Watch the video onoverlap with AN2 disappears. MACS Antibodies Brilliant fluorochromes and high quality antibodies for brighter staining and even better data, particularly for flow cytometry. Choose from a large panel of antibodies against mouse, rat and human antigens. For neural markers please visit to download the complete antibody list 9
  10. 10. Cell culture The medium makes the difference The MACS Cell Culture product line for neuroscience includes A specially formulated cell culture medium, cytokines and growth factors. Get the best for your cells and promote their growth and differentiation in vitro , by working with a great team. MACS Supplement B27 PLUS and MACS Neuro Medium • Serum-free supplement for astrocytes, neurons and oligodendrocytes • Based on B27 with optimized components for in vitro propagation of all mouse, rat, or human neural cells • Serum-free cell culture medium • Promotes optimal growth and long-term survival of all B mouse, rat, or human neural cells Related products: Small molecules Laminin Stem cell-specific media Visit for more information Basic media: Visit MACS Cytokines A comprehensive range of cytokines is available for the promotion of neural differentiation and cell maintenance. Figure 14: (A) Astrocytes and (B) oligodendrocytes show excellent growth and morphology in MACS Neuro Medium and MACS • Superior quality including premium and GMP-grade Supplement B27 PLUS, 0.5 mM L-glutamine and 1% PenStrep on poly-D-lysine-coated coverslips. Astrocytes (4 DIV) were isolated from P1 • Standardized high biological activity mice using Anti-ACSA-2 MicroBeads (coming soon), and oligodendrocytes • Convenient packaging with small or bulk fillings were (5 DIV) isolated from P3 mice using Anti-O4-MicroBeads. Astrocytes were stained using Anti-GLAST (ACSA-1) (green) and Anti-GFAP (red). Visit to download the cytokine product list.10
  11. 11. A Figure 16: Neural/neuronal cells differentiation of human iPS cells to B peripheral neurons.Pluripotent human iPS cells were isolated using the TRA-1-60 MicroBead Kit prior to neural induction with dorsomorphin. At day 10 of differentiation, neural crest stem cells were enriched with Neural Crest Stem Cell MicroBeads. Differentiation to peripheral neurons was performd in MACS Neuro Medium with N2 Supplement, NGF, BDNF, dcAMP and ascorbic acid for three weeks. For more detailed information visit and download poster 4.Figure 15: Whole mouse brain was dissociated using the Neural TissueDissociation Kit (P) and the gentleMACS Dissociator. Neural stem cellswere isolated with Anti-Prominin-1 MicroBeads and subsequently cultivatedin MACS Neuro Medium supplemented with MACS Supplement B27 PLUS,penicillin/streptomycin, 2 mM L-glutamine, 20 ng/mL MACS CytokinesHuman EGF and Human FGF-2. Primary neurospheres formed after oneweek in culture. (A) The Neurosphere Dissociation Kit (P) was used for thedissociation of primary neurospheres. (B) Secondary neurospheres formedin the same medium and cytokines. 11
  12. 12. Neuroimaging Insights into neural function Small animal imaging (SAI) techniques are used to investigate models of human disease, such as Alzheimer’s Disease. Viscover quality State-of-the-art instrumentation coupled with sensitive and • Ready-to-use with step-by-step instructions specific contrast agents, such as the Viscover™ range of • Excellent tolerability – only 100 μL injection volume pre-clinical contrast agents, allow researches to obtain high-fidelity anatomical and functional information from • Translatable to the clinic animal studies in vivo. • Iso-osmolar sterile formulation • Single dose bolus administration Modality Product Applications SAI techniques have evolved from human applications in the group clinic, including magnetic resonance imaging (MRI), computer MRI GadoSpin™ • Brain tumors tomography (CT), ultrasound (US) and optical imaging (OI). Of • Compromised BBB these, MRI and OI are frequently used in neuroscience • Neuroinflammation investigations in small animals although there are Viscover • Stroke products to suit each application: • MS • • Angiography DCE measurements Magnetic resonance imaging • Angiogenesis GadoSpin™ contrast agents are based on gadolinium chelate • Molecular targeted imaging and can be used to examine angiogenesis in brain tumors and FeraSpin™ • Different size-selected nanoparticles neuroinflammation where the blood-brain barrier has been • CNS inflammation compromised. • Osteomyelitis and aseptic vertebral inflammation • Stroke • MS • Alzheimer disease • Angiography • rCBV Figure 17: Intracranial tumor in mouse exhibits contrast enhancement • Microvessel density imaging after GadoSpin M has passed the compromised blood-brain barrier. • Cell tracking The image series shows tumor progression. • Molecular targeted imaging FeraTrack™ Ready-to-use iron oxide particles for ex vivo intracellular labelling of cells prior to transplantation and tracking CT ExiTron™ • Soft tissue contrast • Angiography • Angiogenesis Ultrasound PolySon™ • Perfusion studies • Molecular targeted imaging (see figure 22) Optical NiroWave™ • CNS inflammation Imaging • Stroke • MS • Alzheimers disease • Angiography • Vascular leakage Figure 18: MRI image of mouse after intracerebral injection of GadoSpin F and intravenous injection of Gadospin D and FeraSpin XS. • Angiogenesis Image shows brain and spinal cord (purple) and the vasculature (red). • Brain tumor Table 3: SAI applications and Viscover imaging agents12
  13. 13. FeraSpin™ contrast agents are based on iron oxidenanoparticles and can be used for various neuroscience Optical imagingapplications such as CNS inflammation studies, MRA, rCBV NiraWave™ optical imaging contrast reagents enable the studymeasurements or cell tracking. of disease-related changes in vascular permeability, for example, the breakdown of the blood-brain barrier. NiraWave can also be conjugated to antibodies in the same way as fluorochromes, to enable targeting.Figure 19: Mouse whole-body T1-weighted MR angiography using FeraSpinXS.The FeraTrack tracking kit simplifies intracellular labeling of cells Figure 21: Optical angiography in mouse injected with NiraWave nanoex vivo. Cells labeled with FeraTrack can then be tracked in real 780. The fine structure of blood vessels is visible.time by magnetic resonance imaging. Ultrasound imaging PolySon™ Ultrasound microbubbles have been used, for example, to detect the distribution of lesions and to quantify the expression of ICAM-1 in the study of experimental allergic encephalitis in the mouse:Figure 20: In vivo MRI imaging of mouse cortex: Extracellularly andintracellularly FeraTrack™ labeled PSA-NCAM + cells were grafted intothe cortex of a mouse. A strong contrast was detected for the FeraTrack™labeled cells in the left cortex at 7 Tesla MRI (Prof M. Hoehn, Max PlanckInstitute for Neurological Research, Cologne). Find out more at Figure 22: Detection of compromised BBB in EAE mouse model by i.v. injection of anti-ICAM-1 conjugated PolySon H microbubbles using Ultrasound Imaging: Anti-ICAM-1-PolySonH binds specifically on the surface of lesions that express ICAM-1. 13
  14. 14. Molecular analysis Look below the cell surface Experience how MACSmolecular provides exquisite sensitivity in cDNA synthesis, outstanding purity in protein isolation, high Genomic Services - make the most of recovery in mitochondria isolation, and enables expression small samples profiling of both microRNA and mRNA. If sample material is minimal or is only available as formalin- fixed paraffin-embedded tissue, Genomic Services can extract More than just a power house that valuable information and provide you with the data you are seeking: • SuperAmp: analysis of 10-10,000 cells by million-fold amplification • microRNA expression profiling based on miRXplore™ Microarrays • Agilent whole genome expression or array-CGH analysis and bioinformatics • Cell characterization, disease biology, biomarker identification Send us your sample and we’ll send you documented gene expression data by return. Visit us at Are you studying the role of mitochondria in neurodegenerative disease or aging? The Mitochondria Isolation Kit, human and the Mitochondria Isolation Kit, mouse tissue, are based on renowned MACS Technology (see page 6) and enable: • Pure, intact, viable mitochondria in less than two hours • Higher yields of mitochondria compared to differential centrifugation or density gradient centrifugation • Organelle integrity maintained for full functionality Watch the Mitochondria Isolation Kit in action. Visit μMACS™ and MultiMACS™ cDNA For more information on our entire molecular range visit Synthesis Kits • Time-saving with one step instead of three: combination of mRNA isolation with in-column cDNA synthesis. With the cDNA magnetically bound to the column, no extra cDNA purification step is needed. • High yields • Ready-to-use enzyme reaction mixes in the complete kit14
  15. 15. Further informationSupport and links Technical support We are well known for the high level of support our team of experts provides our customers. We can help with any question you have about our products by: • On-line live technical support (New) • Email • Telephone • On-line discussion forums There are also many articles available for download including • Scientific posters • MACSmore – Neuroscience Special • Datasheets Simply visit We also run customer training at our HQ near Cologne, Germany and on-line customer webinars For ordering information and the latest news on our products: The proof is in the publications! Visit for the extensive list of literature on successful research conducted with our neuroscience products. 15
  16. 16. Germany/Austria/ Australia China Italy Spain 130-091-167.15 Switzerland Miltenyi Biotec Miltenyi Biotec GmbH Miltenyi Biotec S.r.l. Miltenyi Biotec S.L. Miltenyi Biotec GmbH Australia Pty. Ltd. Shanghai Office Via Persicetana, 2/D C/Luis Buñuel 2 Friedrich-Ebert-Straße 68 Unit 16A , 2 Eden Park Drive Rm. 2309–2310, 40012 Calderara di Reno (BO) Ciudad de la Imagen 51429 Bergisch Gladbach North Ryde, NSW 2113, No. 319 Xianxia Rd. Italy 28223 Pozuelo de Alarcón Germany Australia Shanghai 200051, P.R. China Phone +39 051 6 460 411 (Madrid), Spain Phone +49 2204 8306-0 Phone +61 2 8877 7400 Phone +86 21 62351005 Fax +39 051 6 460 499 Phone +34 91 512 12 90 Fax +49 2204 85197 Fax +61 2 9889 5044 Fax +86 21 62350953 Fax +34 91 512 12 91 Japan USA/Canada Benelux France Miltenyi Biotec K.K. United Kingdom Miltenyi Biotec Inc. Miltenyi Biotec B.V. Miltenyi Biotec SAS Nittsu-Eitai Building 5F Miltenyi Biotec Ltd. 2303 Lindbergh Street Schipholweg 68 H, 2316 Leiden 10 rue Mercoeur 16-10 Fuyuki, Koto-ku, Almac House, Church Lane Auburn, CA 95602, USA The Netherlands 75011 Paris, France Tokyo 135-0041, Japan Bisley, Surrey GU24 9DR, UK Phone 800 FOR MACS Phone +33 1 56 98 16 16 Phone +81 3 5646 8910 Phone +44 1483 799 800 Phone +1 530 888 8871 Fax +33 1 56 98 16 17 Fax +81 3 5646 8911 Fax +44 1483 799 811 Fax +1 530 888 8925 Customer service Netherlands Phone 0800 4020120 Fax 0800 4020100 Singapore Customer service Belgium Miltenyi Biotec Phone 0800 94016 Asia Pacific Pte Ltd. Fax 0800 99626 100 Beach Road Customer service Luxembourg #28-06 to 28-08 Shaw Tower Phone 800 24971 Singapore 189702 Fax 800 24984 Phone +65 6238 8183 Fax +65 6238 0302 Miltenyi Biotec provides products and services worldwide. Visit to find your nearest Miltenyi Biotec contact. Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, gentleMACS, gentleMACS Octo, AutoMACS Pro, μMACS, MultiMACS, MACSQuant, Viscover and the MACS Logo are registered trademarks or trademarks of Miltenyi Biotec GmbH. Copyright © 2011 Miltenyi Biotec GmbH. All rights reserved.1