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NCARE Genebank &
Herbarium Database
Ziad Tahabsom
Gene bank documentation supervisor
Genetic Resources Unit
Biodiversity Directorate
National Center for Agricultural
Research and Extension (NCARE(
Amman - Jordan NCARE – November,2013
• The Genebank at NCARE
was established in 1993.
NCARE Genebank
• During 1996 the Genebank was
fully equipped and started
conservation activities
NCARE – November,2013
Methodology
There are three main themes
of the seed banking procedure:
• Planning and collecting.
• Processing and testing.
• storage and utilization.
NCARE – November,2013
Planning and collecting
• Planning and permission seeking.
• Field collection of labeled seeds and
voucher (pressed) specimens plus field
data.
• Rapid shipment of the seeds &
herbarium samples to the Genebank
NCARE – November,2013
processing and testing
• Unpacking of consignments, check for
problems, drying / mounting of vouchers
• Data record creation about each accession
• Seed cleaning
• Seed quantity determination
• Drying
• Determination of seed moisture status
NCARE – November,2013
storage and utilization
• Packaging
• Storage under cold conditions
• Distribution to users (through time)
• Characterization and evaluation
• Germination test (through time)
• Regeneration / Multiplication of
collections (when required)
NCARE – November,2013
The Genebank conserves 3993
seed accessions representing:
• 89 Families
• 435 Genus
• 661 species
Mediterranean
Saharo-ArabianSudanian
Irano-turranien
NCARE – November,2013
NCARE – November,2013
Distribution of seeds from the
Genebank since 1999
• 1725 different accessions
• 76670 seeds
These seeds were used for:
• M.Sc. Researches
• Ph.D. Researches
• Evaluation & characterization
• Basic science researches in Universities and
different research institutions.
NCARE – November,2013
Genebank & Herbarium
Database (interpretation(
• Information system to help running and
sustaining genetic resources
conservation and utilization
Collection field Guide
NCARE – November,2013
Genebank database
Main features
• Focus on data documenting accessions
(seeds, Herbaria) conserved in long-
and short-terms
NCARE – November,2013
Genebank database
Main features
• Focus on “operational” data
– supports management of collections
– supports planning of future work
NCARE – November,2013
Genebank database
Main features
• Multi-user
– all staff has daily access
– on network
NCARE – November,2013
Genebank database:
Main features (cont.(
• Flexibility in querying
– by pre-defined or built by users
– recurrent querying
NCARE – April,2011NCARE – November,2013
Genebank database:
Main features (cont.(
• Flexibility to “export” data and/or to link to other
data analysis packages, e.g. GIS
NCARE – November,2013
Genebank database:
Main features (cont.(
• Flexibility in “importing” data from different sources
NCARE – November,2013
Genebank database:
Main features (cont.(
• Open-ended
system easy
to accommodate
new data.
• Adherence to
international data
standards
(descriptors(
NCARE – November,2013
Genebank database:
considerations
• Backbone: commercial Data Base Management
System (FoxPro(.
NCARE – November,2013
Genebank database:
considerations
• Database design
defined by all staff.
• One comprehensive
database is better
than many
scattered ones.
NCARE – November,2013
Examples of more than two
databases
• Database supporting ex-situ conservation of
genetic resources at GRU of NCARE.
• Database of botanical surveys for Medicinal
Plants Project.
• Database supporting Herbarium samples
conserved at the Herbarium at GRU of NCARE
NCARE – November,2013
Genebank database
Available data
• Passport - the most important category.
• Characterization and preliminary evaluation -
routine for major crops.
• Stock - quality and quantity of seeds.
• Distribution.
• Other: taxonomy, collaborators, etc. NCARE – November,2013
Genebank database layers
Common
data
Barley
Vicia sp.
NCARE – November,2013
Genebank database design
NCARE – November,2013
Working Tools
NCARE – November,2013
Data Sheet for
collection of
various information
NCARE – November,2013
Herbarium specimens are consulted sometimes
NCARE – November,2013
Well prepared pressers are a must
NCARE – November,2013
Possible Target plant (collectable???(
NCARE – November,2013
•Identification in the field
•Special notes
•Colorful simple booklets
NCARE – November,2013
Sometimes easily reached
NCARE – November,2013
But not always easy
NCARE – November,2013
Mission Impossible!!
But we are going
to try….
NCARE – November,2013
Places and Habitats
visited
NCARE – November,2013
Major threats of Jordan
natural eco-systems
urbanization
Over grazing
DeforestationDepletion of major
water resources
agricultural development
Illegal collection
NCARE – November,2013
Seed Collection
NCARE – November,2013
Whole plant
should be
pulled out for a
representative
herbarium
specimen
NCARE – November,2013
Identification of
Herbarium
Specimens
NCARE – November,2013
Seeds are let to equilibrate
with dry air to lose moisture
NCARE – November,2013
Labeled seed bags containing collected
seeds ready for shipment or banking
NCARE – November,2013
Seed Banking
NCARE – November,2013
Herbarium specimen
Mounted And labeled
Jordan National Center
Herbarium
JNCH
Family: Resedaceae
Scientific name: Reseda sp
Vernacular name:
Locality: Dead Sea area, Wadi
for rain water, Just after
Amman municipality tourism
sites
Habitat: open road side area,
Sodanoturanian
Date: 29/ 3/ 2005
Altitude: -400 m
Identified by: Khaled Abulaila
Collector(s):KA&ZT No:
NCARE – November,2013
Herbarium specimens preserved in cabinet
according to family, Genera and species
NCARE – November,2013
Databasing information
NCARE – November,2013
Source: Jordan country study on biological diversity 1998.
Some selected endemic, rare, endangered
and extinct flora of Jordan
NCARE – November,2013
Hyphaene thebaica
Sternbergia clusiana
Iris nigricans
Ophrys umbilicata
Astragalus adpressiusculus
Maerua crassifolia
Salvia horminum
Himantoglossum affine
Moringa perigrina
A Selection of Flora preserved as Herbarium and Seeds at
NCARE genebank and herbarium
NCARE – November,2013
Cyclamen palastinus
NCARE – November,2013
Thank you
NCARE – November,2013

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NCARE Genebank Database Guide