StableLab is a new handheld blood test that measures Serum Amyloid A (SAA), an acute phase protein biomarker of inflammation and infection in horses. The document describes a case where StableLab was used to monitor the disease progression and response to antibiotic treatment of a horse with travel sickness. Over 13 days, the horse's SAA levels gradually decreased from 379.5 μg/ml to 39 μg/ml as its clinical signs and lung condition improved in response to the antibiotic treatment. Veterinarians comment that StableLab is useful as an early indicator of infection and for monitoring treatment effectiveness at the horse's side, without waiting for laboratory results.
Doorlopend voert Kamernet.nl onderzoek uit binnen haar grote database van kamers en haar klanten doelgroep: kamerzoekers en kamerverhuurders, om meer inzicht in de ontwikkelingen van de kamermarkt te krijgen zodat wij de dienstverlening van Kamernet.nl verder kunnen optimaliseren.
Op het blog van Kamernet.nl, http://blog.kamernet.nl, zijn alle marktrapporten terug te vinden. Van de grootste 8 steden van Nederland is op http://blog.kamernet.nl eenzelfde marktrapport aanwezig.
‘This year the pain of market fluctuations was felt by all, right from the small retail investor to big corporates,’ said Jagannadham Thunuguntla, head of the capital markets arm with SMC Group.
Doorlopend voert Kamernet.nl onderzoek uit binnen haar grote database van kamers en haar klanten doelgroep: kamerzoekers en kamerverhuurders, om meer inzicht in de ontwikkelingen van de kamermarkt te krijgen zodat wij de dienstverlening van Kamernet.nl verder kunnen optimaliseren.
Op het blog van Kamernet.nl, http://blog.kamernet.nl, zijn alle marktrapporten terug te vinden. Van de grootste 8 steden van Nederland is op http://blog.kamernet.nl eenzelfde marktrapport aanwezig.
‘This year the pain of market fluctuations was felt by all, right from the small retail investor to big corporates,’ said Jagannadham Thunuguntla, head of the capital markets arm with SMC Group.
Doorlopend voert Kamernet.nl onderzoek uit binnen haar grote database van kamers en haar klanten doelgroep: kamerzoekers en kamerverhuurders, om meer inzicht in de ontwikkelingen van de kamermarkt te krijgen zodat wij de dienstverlening van Kamernet.nl verder kunnen optimaliseren.
Op het blog van Kamernet.nl, http://blog.kamernet.nl, zijn alle marktrapporten terug te vinden. Van de grootste 8 steden van Nederland is op http://blog.kamernet.nl eenzelfde marktrapport aanwezig.
Background: It is often difficult to predict which newborn with HIE will develop neurological sequlae so there is an urgent need for predictors for adverse neurological outcomes in these infants. Aim of Study: To evaluate the serum levels of serum amyloid A (SAA) protein in newborns with HIE during the first week of life and after 3 and 6 months of follow up to assess its correlation with degree of HIE neurological sequlee. Patients and Methods; This case-control study was conducted on 72 infants; group (1) included 36 full term neonates diagnosed as HIE and group (2)included 36 age and sex matched, infants as a control group, Serum amyloid A by ELIZA technique was measured at post natal age of 1 and 7 days, CT scan was done in justified cases .with follow up at age of 3 and 6 months for neurological sequlee. Results: SAA protein level was elevated in the asphyxiated group in comparison to the control group at day 1 and day 7, SAA level was significantly correlated to the Sarnat scoring system of HIE. SAA level significantly differ on follow up of developmental milestone at age of 3 and 6 months. ROC curve for validity of SAA for severity of HIE at cut off point > 25μg/ml at day 1 and at cut off point > 20 μg/ml at day 7 of HIE diagnosis reported sensitivity 100% and specificity 100% .Conclusion: SAA correlates with the severity of HIE and higher SAA expression is a prognostic marker for morbidity in these infants.
Immunoassay basic concepts for clinical pathologistDr. Rajesh Bendre
Immunoassays as technique have evolved considerably since the invention of Radioimmunoassay, monoclonal antibody, Recombinant technology & successfully achieved automation. However, many of the hormonal assays still lack standardization and/or Harmonization resulting in significant variability in test results. Using alternate methods, adopting procedures for sample pre-treatment, serial dilution of sample are some of the ways to troubleshoot these discrepant result
Rhogam is a sterilized solution that is made from human blood that contains a very small amount of Rhesus positive protein.
These proteins usually prevent the body from making permanent antibodies that reacts against the rhesus positive. It is administered as an intravenous injection.
Mixing of maternal and fetus usually occurs in the last trimester of pregnancy due to continuous thinning of the membrane that separates the fetus blood and that of the mother and also due to the growing placenta, it is due to this reason that Rhogam is given at 28th week of pregnancy to protect the mother for the rest of pregnancies.
Doorlopend voert Kamernet.nl onderzoek uit binnen haar grote database van kamers en haar klanten doelgroep: kamerzoekers en kamerverhuurders, om meer inzicht in de ontwikkelingen van de kamermarkt te krijgen zodat wij de dienstverlening van Kamernet.nl verder kunnen optimaliseren.
Op het blog van Kamernet.nl, http://blog.kamernet.nl, zijn alle marktrapporten terug te vinden. Van de grootste 8 steden van Nederland is op http://blog.kamernet.nl eenzelfde marktrapport aanwezig.
Background: It is often difficult to predict which newborn with HIE will develop neurological sequlae so there is an urgent need for predictors for adverse neurological outcomes in these infants. Aim of Study: To evaluate the serum levels of serum amyloid A (SAA) protein in newborns with HIE during the first week of life and after 3 and 6 months of follow up to assess its correlation with degree of HIE neurological sequlee. Patients and Methods; This case-control study was conducted on 72 infants; group (1) included 36 full term neonates diagnosed as HIE and group (2)included 36 age and sex matched, infants as a control group, Serum amyloid A by ELIZA technique was measured at post natal age of 1 and 7 days, CT scan was done in justified cases .with follow up at age of 3 and 6 months for neurological sequlee. Results: SAA protein level was elevated in the asphyxiated group in comparison to the control group at day 1 and day 7, SAA level was significantly correlated to the Sarnat scoring system of HIE. SAA level significantly differ on follow up of developmental milestone at age of 3 and 6 months. ROC curve for validity of SAA for severity of HIE at cut off point > 25μg/ml at day 1 and at cut off point > 20 μg/ml at day 7 of HIE diagnosis reported sensitivity 100% and specificity 100% .Conclusion: SAA correlates with the severity of HIE and higher SAA expression is a prognostic marker for morbidity in these infants.
Immunoassay basic concepts for clinical pathologistDr. Rajesh Bendre
Immunoassays as technique have evolved considerably since the invention of Radioimmunoassay, monoclonal antibody, Recombinant technology & successfully achieved automation. However, many of the hormonal assays still lack standardization and/or Harmonization resulting in significant variability in test results. Using alternate methods, adopting procedures for sample pre-treatment, serial dilution of sample are some of the ways to troubleshoot these discrepant result
Rhogam is a sterilized solution that is made from human blood that contains a very small amount of Rhesus positive protein.
These proteins usually prevent the body from making permanent antibodies that reacts against the rhesus positive. It is administered as an intravenous injection.
Mixing of maternal and fetus usually occurs in the last trimester of pregnancy due to continuous thinning of the membrane that separates the fetus blood and that of the mother and also due to the growing placenta, it is due to this reason that Rhogam is given at 28th week of pregnancy to protect the mother for the rest of pregnancies.
Elabscience is passionate about cancer research; each of ELISA kits is created with careful consideration for the relevance and specificity of the targets as well as the necessity of validated applications. Check the ELISA kits list for your cancer research. Any other problem, please feel free to contact our technical support or visit our site: http://www.elabscience.com
Common Lab Investigations in pregnancy specific to each TrimesterDrNisheethOza
There are no standardized guidelines/protocols for conducting common laboratory investigations during pregnancy. Here is an attempt to educate Pregnant ladies in this important aspect of their healthcare.
If you want to understand how decisions are made at a VC firm it is important to understand the staff who work there. Here is a guide but you can also read more at this blog post: https://bothsidesofthetable.com/how-to-improve-your-odds-of-getting-to-yes-with-a-vc-land-and-expand-b46a0a102a07
2. Monitoring
Disease
Progression and
Response to
Treatment
In cases where Serum Amyloid A is
detected as a biomarker of disease,
StableLab can be used as a tool to
monitor disease progression and
response to treatment. Detailed
here is an example of where
StableLab was used in this manner.
The SAA levels of a horse that
presented with travel sickness
(pleuropneumonia) were measured
daily for 13 days following a 6689
kilometre transportation (Table
1, Figure 1). Clinical examination,
SAA testing and lung scanning
were carried out by an ambulatory
veterinarian.
The horse was treated with
Ceftiofur (antibiotic), Marbofloxacin
(antibiotic), Flunixin (anti-
inflammatory) Metronidazole
(antibiotic) and Gastrogard
(treatment/prevention of equine
ulcers).
table 1 saa measurements and clinical assessment of a horse with travel sickness.
figure 1 Graphical representation of the data in Table 1
1 379.5 Temp 102, thin, dull. Fluid on right hand side chest and
consolidated lung.
2 3493 Improved condition, temp 101. Lung consolidation.
3 3285 Temp normal
4 3384 Temp normal, consolidation resolving
5 3112 Temp normal, consolidation resolving
6 2640 Temp normal, consolidation resolving, reduced flunixin
7 3960 Temp normal, consolidation resolving,
reduced flunixin
8 2755 Gas pocket in neck, changed ceftiofur to cefquinome
9 838 Scan much improved
10 475 Temp consistent 99.8
11 199 Temp consistent 99.8
12 101 Temp consistent 99.4, stop flunixin
13 39 Scan improved, small comet tails, stop marbocyl
Day saa μg/ml Veterinarian Comments
4500
4000
3500
3000
2500
2000
1500
1000
500
0
1 2 3 4 5 6 7 8 9 10 11 12 13
saa (μg/ml)
days
What is
StableLab?
StableLab detects and quantifies
a protein in blood called Serum
Amyloid A. Serum Amyloid A is
normally absent, or only present
in very low concentrations in
healthy horses, but increases
rapidly and dramatically in
response to infection and certain
inflammatory conditions. The
severity of disease is reflected in
the level of SAA elevation, making
StableLab an excellent health
management tool.
This is the StableLab cartridge —>
This line at “C” always appears —
it shows that the test is working.
A second line appears at “T”
if SAA is present in your horse .
You can read the test
result by eye against a
reference card, or use our
handheld reader (see back
page).
Treatment changed here
Monitoring
Disease
Progression and
Response to
Treatment
StableLab can be used as a tool to
monitor disease progression and
response to treatment. Detailed
here is an example of where
StableLab was used in this manner.
The SAA levels of a horse that
presented with travel sickness
(pleuropneumonia) were measured
daily for 13 days following a 6689
kilometre transportation (Table
1, Figure 1). Clinical examination,
SAA testing and lung scanning
were carried out by an ambulatory
veterinarian.
The horse was treated with
Ceftiofur (antibiotic), Marbofloxacin
(antibiotic), Flunixin (anti-
inflammatory) Metronidazole
(antibiotic) and Gastrogard
(treatment/prevention of equine
ulcers).
table 1 saa measurements and clinical assessment of a horse with travel sickness.
figure 1 Graphical representation of the data in Table 1
1 379.5 Temp 102, thin, dull. Fluid on right hand side chest and
consolidated lung.
2 3493 Improved condition, temp 101. Lung consolidation.
3 3285 Temp normal
4 3384 Temp normal, consolidation resolving
5 3112 Temp normal, consolidation resolving
6 2640 Temp normal, consolidation resolving, reduced flunixin
7 3960 Temp normal, consolidation resolving,
reduced flunixin
8 2755 Gas pocket in neck, changed ceftiofur to cefquinome
9 838 Scan much improved
10 475 Temp consistent 99.8
11 199 Temp consistent 99.8
12 101 Temp consistent 99.4, stop flunixin
13 39 Scan improved, small comet tails, stop marbocyl
Day saa μg/ml Veterinarian Comments
4500
4000
3500
3000
2500
2000
1500
1000
500
0
1 2 3 4 5 6 7 8 9 10 11 12 13
saa(μg/ml)
days
What is
StableLab?
StableLab is a new blood test
that’s different in three ways:
it’s handheld, it measures a new
biomarker called SAA, and it
works more like a thermometer
than a lab report, helping you
understand if a horse is sick or
not, right at the horse’s side.
StableLab can be used to detect
infections in horses and it also
tells you if antibiotic treatment
is working.
Treatment changed here
3. How we read it
• We use StableLab when presented with a horse that
has a potential illness.
• A clear result, with no visible test-line indicates
that the horse most likely does not have a systemic
infection.
• If the result is reported as positive and:
– Less than 50 μg/ml, we may continue to monitor
the patient before initiating therapy OR we
may discontinue therapy if the SAA was initially
elevated and the patient finished its course of
treatments.
– Between 50 μg/ml and 200ug/ml we begin
appropriate diagnostics and /or treatments
depending on our physical exam findings.
– Greater than 200 μg/ml indicates the animal most
likely has some sort of systemic illness that will
require more diagnostics and /or treatments.
• On certain cases we will use StableLab to monitor
response to treatments.
recommended protocols
Dr. Nathan Slovis
dvm, dacvim, cht
“We find that it is an earlier and more sensitive
indicator of equine inflammatory and infectious
diseases than other lab methods. StableLab is
a breakthrough in stall side equine diagnostics,
and presents a new way of blood testing.”
When we use it
We use StableLab as an adjunct diagnostic to
help identify horses that may have a systemic
infection. We also use StableLab to monitor
our patients and how they are responding to
our therapies.
Serum Amyloid A (SAA) is a rapid response
protein with a half-life of approximately 24
hours, it’s the earliest indicator of systemic
inflammation and/or infection.
StableLab helps those who care for the
horse’s well-being by offering testing in an
easy and convenient manner. An increasing
value on StableLab informs our veterinarians
that a disease process may be occurring
and we can respond appropriately (i.e. more
diagnostics or initiate treatment based on
our physical exam) when we see the values
reduce, we gain confidence that our patient is
responding to treatment.
StableLab’s Handheld Reader
The StableLab handheld reader accurately
measures SAA from 0–3000 μg/ml.Take
the StableLab reader with you to record and
track your results anytime, anywhere.