SHAPE Society

1. Cytokine-SPIO Protocol
•Purpose: To compare the iron particles present in macrophages of
apo E-deficient atherosclerotic plaques under baseline conditions
(control group) and after the administration of the cytokines TNF-alpha
and IL-1beta (test group).
•Protocol: Eight retired female breeders, approximately 12-month old
were divided in 2 groups. Five received I.P. 0.2 µg each of mouse
recombinant TNF- and IL-1ß; the 3 control received 0.5 mL saline
containing 1% BSA. Three hours later, all the animals were I.V.
injected with Feridex 1 mMol/kg of iron. Seven days later, the recipients
were euthanized with CO2 and perfused under physiological pressure.
The entire aorta up to the iliac bifurcation was formalin-fixed,
serially sectioned transversally and stained for H&E and iron.

2. No cytokines
C1Cx10

3. No cytokines
C1Cx40

4. No cytokines
C3Ex10

5. No cytokines
Only plaque with Fe
C3Ex40

6. No cytokines
C3E

7. TNFα/IL-1ß
T2Fx10

8. TNFα/IL-1ß
T2Fx40

9. TNFα/IL-1ß
T2Fx40ab

10. TNFα/IL-1ß
T2Fx40cd

11. TNFα/IL-1ß
T3Fx10

12. Coronary artery involvement
T3F coronary

13. TNFα/IL-1ß
T3Fx40

14. TNFα/IL-1ß
T4Bx10

15. TNFα/IL-1ß
T4Bx40

16. TNFα/IL-1ß
T4B

17. TNFα/IL-1ß
T4F

18. TNFα/IL-1ß
T4Cx10

19. TNFα/IL-1ß
T4Cx40

20. TNFα/IL-1ß
T4Fx10

21. TNFα/IL-1ß
T4Fx10bc

22. Infarct and coronary involvement
T4F

23. TNFα/IL-1ß
T4Fx40bcd

24. TNFα/IL-1ß
T3Ex10

25. TNFα/IL-1ß
T3Efex40

26. TNFα/IL-1ß
T3Bx10

27. TNFα/IL-1ß
T3Bx40ab

28. TNFα/IL-1ß
T3Bfex40ab

29. TNFα/IL-1ß
T4E