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Cytokine-SPIO Protocol
•Purpose: To enhance macrophage homing to plaque and
monitor it by SPIO. Compare the iron particles...
No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining
No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining
No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining
No cytokines SPIO Injected Apo E
Only plaque with SPIO C3Ex40
No Cytokines SPIO Injected Apo E
C3E
SPIO Found in Circulating Monocytes
Iron Staining
H&E Staining
T2Fx10
TNFα/IL-1ß SPIO Injected Apo E
Iron Staining H&E Staining
T2Fx10
TNFα/IL-1ß SPIO Injected Apo E
H&E StainingH&E Staining
TNFα/IL-1ß
T2Fx40ab
TNFα/IL-1ß
T2Fx40cd
TNFα/IL-1ß
T3Fx10
TNFα/IL-1ß
T3Fx40
TNFα/IL-1ß
T4Bx10
TNFα/IL-1ß
T4Bx40
TNFα/IL-1ß
T4B
TNFα/IL-1ß
T4F
TNFα/IL-1ß
T4Cx10
TNFα/IL-1ß
T4Cx40
TNFα/IL-1ß
T4Fx10
TNFα/IL-1ß
T4Fx10bc
Infarct and coronary involvement
T4F
TNFα/IL-1ß
T4Fx40bcd
TNFα/IL-1ß
T3Ex10
TNFα/IL-1ß
T3Efex40
TNFα/IL-1ß
T3Bx10
TNFα/IL-1ß
T3Bx40ab
TNFα/IL-1ß
T3Bfex40ab
TNFα/IL-1ß
T4E
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Spio cytokinejan28-2

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Spio cytokinejan28-2

  1. 1. Cytokine-SPIO Protocol •Purpose: To enhance macrophage homing to plaque and monitor it by SPIO. Compare the iron particles present in macrophages of apo E-deficient atherosclerotic plaques under baseline conditions (control group) and after the administration of the cytokines TNF-alpha and IL-1beta (influenza simulated group). •Protocol: Eight retired female breeders, approximately 12-month old were divided in 2 groups. Five received I.P. 0.2 µg each of mouse recombinant TNF- and IL-1ß; the 3 control received 0.5 mL saline containing1% BSA. Three hours later, all the animals were I.V. injected with Feridex 1 mMol/kg of iron. Seven days later, the recipients were euthanized with CO2 and perfused under physiological pressure. The entire aorta up to the iliac bifurcation was formalin-fixed, serially sectioned transversally and stained for H&E and iron.
  2. 2. No cytokines C1Cx10 Apo E SPIO Injected Iron Staining H&E Staining
  3. 3. No cytokines C1Cx10 Apo E SPIO Injected Iron Staining H&E Staining
  4. 4. No cytokines C1Cx10 Apo E SPIO Injected Iron Staining H&E Staining
  5. 5. No cytokines SPIO Injected Apo E Only plaque with SPIO C3Ex40
  6. 6. No Cytokines SPIO Injected Apo E C3E SPIO Found in Circulating Monocytes Iron Staining H&E Staining
  7. 7. T2Fx10 TNFα/IL-1ß SPIO Injected Apo E Iron Staining H&E Staining
  8. 8. T2Fx10 TNFα/IL-1ß SPIO Injected Apo E H&E StainingH&E Staining
  9. 9. TNFα/IL-1ß T2Fx40ab
  10. 10. TNFα/IL-1ß T2Fx40cd
  11. 11. TNFα/IL-1ß T3Fx10
  12. 12. TNFα/IL-1ß T3Fx40
  13. 13. TNFα/IL-1ß T4Bx10
  14. 14. TNFα/IL-1ß T4Bx40
  15. 15. TNFα/IL-1ß T4B
  16. 16. TNFα/IL-1ß T4F
  17. 17. TNFα/IL-1ß T4Cx10
  18. 18. TNFα/IL-1ß T4Cx40
  19. 19. TNFα/IL-1ß T4Fx10
  20. 20. TNFα/IL-1ß T4Fx10bc
  21. 21. Infarct and coronary involvement T4F
  22. 22. TNFα/IL-1ß T4Fx40bcd
  23. 23. TNFα/IL-1ß T3Ex10
  24. 24. TNFα/IL-1ß T3Efex40
  25. 25. TNFα/IL-1ß T3Bx10
  26. 26. TNFα/IL-1ß T3Bx40ab
  27. 27. TNFα/IL-1ß T3Bfex40ab
  28. 28. TNFα/IL-1ß T4E

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