SHAPE Society

1. Cytokine-SPIO Protocol
•Purpose: To enhance macrophage homing to plaque and
monitor it by SPIO. Compare the iron particles present in
macrophages of apo E-deficient atherosclerotic plaques under baseline
conditions (control group) and after the administration of the cytokines
TNF-alpha and IL-1beta (influenza simulated group).
•Protocol: Eight retired female breeders, approximately 12-month old
were divided in 2 groups. Five received I.P. 0.2 µg each of mouse
recombinant TNF- and IL-1ß; the 3 control received 0.5 mL saline
containing1% BSA. Three hours later, all the animals were I.V.
injected with Feridex 1 mMol/kg of iron. Seven days later, the recipients
were euthanized with CO2 and perfused under physiological pressure.
The entire aorta up to the iliac bifurcation was formalin-fixed,
serially sectioned transversally and stained for H&E and iron.

2. No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining

3. No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining

4. No cytokines
C1Cx10
Apo E SPIO Injected
Iron Staining
H&E Staining

5. No cytokines SPIO Injected Apo E
Only plaque with SPIO C3Ex40

6. No Cytokines SPIO Injected Apo E
C3E
SPIO Found in Circulating Monocytes
Iron Staining
H&E Staining

7. T2Fx10
TNFα/IL-1ß SPIO Injected Apo E
Iron Staining H&E Staining

8. T2Fx10
TNFα/IL-1ß SPIO Injected Apo E
H&E StainingH&E Staining

9. TNFα/IL-1ß
T2Fx40ab

10. TNFα/IL-1ß
T2Fx40cd

11. TNFα/IL-1ß
T3Fx10

12. TNFα/IL-1ß
T3Fx40

13. TNFα/IL-1ß
T4Bx10

14. TNFα/IL-1ß
T4Bx40

15. TNFα/IL-1ß
T4B

16. TNFα/IL-1ß
T4F

17. TNFα/IL-1ß
T4Cx10

18. TNFα/IL-1ß
T4Cx40

19. TNFα/IL-1ß
T4Fx10

20. TNFα/IL-1ß
T4Fx10bc

21. Infarct and coronary involvement
T4F

22. TNFα/IL-1ß
T4Fx40bcd

23. TNFα/IL-1ß
T3Ex10

24. TNFα/IL-1ß
T3Efex40

25. TNFα/IL-1ß
T3Bx10

26. TNFα/IL-1ß
T3Bx40ab

27. TNFα/IL-1ß
T3Bfex40ab

28. TNFα/IL-1ß
T4E