The SiriusT3 is Sirius Analytical's next-generation system for automated physical-chemical property measurements. It replaces their previous industry-standard GLpKa system with a new modular and miniaturized platform. The SiriusT3 measures properties such as pKas, logPs, and solubility using small sample volumes. It has modules for dispensing, automated sample loading, and titration measurements. The system allows both low-throughput and high-throughput options and can obtain data faster and using less sample than other systems.

1. Next generation solution for
automated physchem measurement

2. The SiriusT3 is the next generation With a new modular and ultra-miniaturised
physchem measurement system from Sirius hardware platform, we have developed a
Analytical. It replaces our world renowned new system which meets the needs of the
and market leading GLpKa system, which pharmaceutical industry from early
over the last decade has been established as discovery all the way through to
the industry standard for pKa, logP/D and late stage formulation
solubility determinations. and development.
The SiriusT3 comprises 3 modules:
Dispenser module, which houses Autoloader module, which has a
the precision micro dispensers worktable with four
for adding water, solvents and 48-position vial trays. The trays
acid/base titrants from the are standard footprint, and
reagent bottles stored in the easily integrated with existing
drawer underneath. The robotics systems. It has a robotic
instrument can have up to 6 arm which automatically picks
different cosolvents loaded up and move vials to the sample
simultaneously (Methanol, Ethanol, position of the Titrator module.
DMSO, Acetonitrile, Dioxane, DMF, THF and At the front of the Autoloader module is a
others). Also contained within this module is a UV/Vis built-in ultrasonic bath, which can be automatically used to
spectrometer and light source, which is connected to a fibre aid the dissolution of poorly soluble compounds.
optic dip probe. This provides the same high quality UV/Vis
spectroscopic functionality as our well established D-PAS
system for the GLpKa.
Titrator module, which features a
moving arm with the assay
probes attached – pH electrode,
UV dip probe, overhead stirrer,
temperature sensor and
capillaries for reagent addition.
All of these probes have been
miniaturised to fit into 1ml
solution volumes. There is a row of
buffer and wash positions used for
automatically calibrating and cleaning the probes, including
a flowing water wash which cleans the electrode with fresh
water after each experiment. At the front of the Titrator
module is the sample position, which is temperature
controlled with a Peltier device (fully controlled by
computer), and an additional turbidity sensing device. This
is the position where the titration for pKa, logP/D or
solubility is performed.

3. SiriusT3 Titrator Module
SiriusT3 Autoloader Module
SiriusT3 Dispenser Module
SiriusT3 measures multiple physchem
properties on a single hardware platform:
pKas, logPs, logDs, Kinetic Solubility and Equilibrium Low Throughput Option
(thermodynamic) Solubility. SiriusT3 Dispenser Module + SiriusT3 Titrator Module
The UV system allows us to also measure pKas by a UV High Throughput Option
method, and also determine Molar Extinction co-efficients. SiriusT3 Dispenser Module + SiriusT3 Titrator Module +
The flexibility of the platform allows scope for new and SiriusT3 Autoloader Module
customized assays to be easily implemented.
Both options are available with and without the UV
SiriusT3 modular design allows customers a clear upgrade spectroscopy system.
route from low to high throughput physchem
measurements: The highest throughput assays on the SiriusT3 can
measure pKas in under 4 minutes, using just 3µL of 10mM
DMSO stock solution. No other system can offer richly
detailed measured pKa data at this speed and small sample
requirement.
The SiriusT3 allows “gold
standard” physchem measurements to
be performed on sub milligram quantities of
material. This revolution allows the best quality
data to be obtained even on discovery compounds
where sample quantity is a limiting factor. Ordering Information:
By obtaining high quality information at an earlier Visit our website for detailed
stage in the discovery and development process, information on how to obtain
decisions about chemistry changes and SiriusT3 for your laboratory
formulation routes can be made early, saving
money, and potential problem areas in other
assays can be indentified and
resolved with ease.

4. Sirius Analytical Ltd.
Riverside, Unit 12, Forest Row Business Park,
Forest Row, East Sussex, RH18 5DW, UK
Telephone: +44 (0) 1342 820720
Fax: +44 (0) 1342 820725
Email: sirius@sirius-analytical.com
web: www.sirius-analytical.com