Calcitonin regulates blood calcium levels and is secreted during implantation under progesterone's influence. The study aimed to determine calcitonin's dose-dependent effects on β-catenin and phospho-β-catenin expressions in trophoblast cells under progesterone and estrogen. Higher calcitonin concentrations increased membrane β-catenin and decreased cytoplasmic phospho-β-catenin, indicating more adherens junctions and less trophoblast invasion. The results suggest calcitonin's usual effect on endometrial epithelial cells changes during implantation under progesterone and estrogen.