1. The study aims to demonstrate that ORF-116, a glycoprotein found in cytomegalovirus (CMV), is required for efficient viral infection of fibroblasts. 2. Various molecular biology techniques are used in the study such as northern blot, PCR-Q, western blot, and immunofluorescence to detect viral sequences, antigens, and analyze protein-antibody interactions. 3. The results found that trans-complementation of ORF-116 restored infectivity, showing that it is a virion-associated glycoprotein necessary for CMV infection of fibroblasts.

1. Isabela Piedrahita Montoya
Isabela Piedrahita Cartagena
Universidad Pontificia Bolivariana
Tercer Semestre

2. INTRODUCCIÓN
● Citomegalovirus
¿Que es?
¿Que produce?
● ORF-116
¿Que es?
¿Qué relación
tiene con el de
citomegalovirus?

3. OBJECTIVE
“We demonstrate that R116
is a virion-associated
glycoprotein that is
required for efficient viral
infection of fibroblasts, and
trans-complementation of
R116 restored infectivity.”

4. MÉTODOS
Northernblot
Detección de secuencias de
ARN
PCR-Q
Antígenos de
microorganismos a los que
se han fijado anticuerpos
específicos marcados con
fluoresceína.
01
03
Western blot
Red de acción entre un
antígeno y anticuerpo
02
04
Imunofluorescencia
Detectan el ADN o el ARN
de un patógeno

5. RESULTADOS

6. INMUNOFLUORESCENCIA

7. Blotting

8. Discusión
AUTHOR QUOTE AGREED OR NOT
Caló et al., 2016 Multiple variants of UL116 have
been described, with a 35 kDa, a
76 kDa, and a 125 kDa form
accumulating after 3 dpi.
✓
Nguyen and Kamil, 2018 So far, gH/gL/gO has been
described as the primary gH
based complex for entry into
fibroblasts, and is believed to
function with gB to create the
core fusion machinery for entry
✓
Feire et al., 2004, 2010;
Soroceanu et al., 2008;
Martinez-Martin et al., 2018;
Wang et al., 2003
Numerous receptors of CMV
envelope glycoproteins have
been identified, conferring a
broad cell tropism to CMV,
including fibroblasts, endothelial
cells, epithelial cells and myeloid
cells.
?

9. Conclusions.
1. The study uses molecular biology methods to learn the behavior of a cell after
being infected with CMV to later be able to determine ways to deactivate said
virus.
2. Molecular biology, as inferred in the article is a key tool in the creation of
vaccines and drugs, since they allow us to understand the compositions of the
different organism present in the environment.
3. To this day there are still many viruses of which we, as the medical community,
have little to no understanding and the pandemic was a perfect example of said
lack of knowledge, but through studies like this one, with the help of different
molecular biology tests and the tools they provide, the medical community has
been able to gain new insights into the structure of these microorganisms.

10. By: Isabela P. Cartagena

11. Whatisit?
Cytomegalovirus
Human cytomegalovirus (CMV), an infectious
agent that is ubiquitous in the world population,
is a member of human herpesvirus family
including viruses such as Epstein-Barr virus,
herpes simplex virus, and varicella zoster.
CMV is the largest virus of the herpes family
Transmision
Human cytomegalovirus, is transmitted through solid
organ/bone transplantation, placental pathway, sexual
intercourse, blood transfusion, and in close contact
with virus-spreading people. Similar to other
herpesviruses, it leads to primary infection, secondary
infection, latent infection, and reactivation or
reinfection.
In immunocompetent adults, primary CMV
infections are usually asymptomatic. Less
commonly, fever, lymphadenopathy, and
mononucleosis-like syndrome coexist with
peripheral lymphocytosis. When people with
impaired immune function are not treated, it can
lead to infections with high mortality
Clinical
manifestations