"Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis ...Nicholas Young
My talk from the American College of Rheumatology Meeting in Boston, MA, November 14-19, 2014: • "Bioluminescent Imaging of Histidyl-Transfer RNA Synthetase-Induced Myositis Reveals Early-Phase Involvement of NF-kB-Mediated Inflammation".
biomarkers in SLE ( Systemic Lupus Erythematosus )Pankaj Ombase
Novel serum and urine biomarkers have been proposed and validated.Combination of novel and traditional disease-specific biomarkers may improve diagnosis and management of SLE. Urinary and serum biomarker along with complement system is explained.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Travis Hills' Endeavors in Minnesota: Fostering Environmental and Economic Pr...Travis Hills MN
Travis Hills of Minnesota developed a method to convert waste into high-value dry fertilizer, significantly enriching soil quality. By providing farmers with a valuable resource derived from waste, Travis Hills helps enhance farm profitability while promoting environmental stewardship. Travis Hills' sustainable practices lead to cost savings and increased revenue for farmers by improving resource efficiency and reducing waste.
DERIVATION OF MODIFIED BERNOULLI EQUATION WITH VISCOUS EFFECTS AND TERMINAL V...Wasswaderrick3
In this book, we use conservation of energy techniques on a fluid element to derive the Modified Bernoulli equation of flow with viscous or friction effects. We derive the general equation of flow/ velocity and then from this we derive the Pouiselle flow equation, the transition flow equation and the turbulent flow equation. In the situations where there are no viscous effects , the equation reduces to the Bernoulli equation. From experimental results, we are able to include other terms in the Bernoulli equation. We also look at cases where pressure gradients exist. We use the Modified Bernoulli equation to derive equations of flow rate for pipes of different cross sectional areas connected together. We also extend our techniques of energy conservation to a sphere falling in a viscous medium under the effect of gravity. We demonstrate Stokes equation of terminal velocity and turbulent flow equation. We look at a way of calculating the time taken for a body to fall in a viscous medium. We also look at the general equation of terminal velocity.
Toxic effects of heavy metals : Lead and Arsenicsanjana502982
Heavy metals are naturally occuring metallic chemical elements that have relatively high density, and are toxic at even low concentrations. All toxic metals are termed as heavy metals irrespective of their atomic mass and density, eg. arsenic, lead, mercury, cadmium, thallium, chromium, etc.
The ability to recreate computational results with minimal effort and actionable metrics provides a solid foundation for scientific research and software development. When people can replicate an analysis at the touch of a button using open-source software, open data, and methods to assess and compare proposals, it significantly eases verification of results, engagement with a diverse range of contributors, and progress. However, we have yet to fully achieve this; there are still many sociotechnical frictions.
Inspired by David Donoho's vision, this talk aims to revisit the three crucial pillars of frictionless reproducibility (data sharing, code sharing, and competitive challenges) with the perspective of deep software variability.
Our observation is that multiple layers — hardware, operating systems, third-party libraries, software versions, input data, compile-time options, and parameters — are subject to variability that exacerbates frictions but is also essential for achieving robust, generalizable results and fostering innovation. I will first review the literature, providing evidence of how the complex variability interactions across these layers affect qualitative and quantitative software properties, thereby complicating the reproduction and replication of scientific studies in various fields.
I will then present some software engineering and AI techniques that can support the strategic exploration of variability spaces. These include the use of abstractions and models (e.g., feature models), sampling strategies (e.g., uniform, random), cost-effective measurements (e.g., incremental build of software configurations), and dimensionality reduction methods (e.g., transfer learning, feature selection, software debloating).
I will finally argue that deep variability is both the problem and solution of frictionless reproducibility, calling the software science community to develop new methods and tools to manage variability and foster reproducibility in software systems.
Exposé invité Journées Nationales du GDR GPL 2024
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
The use of Nauplii and metanauplii artemia in aquaculture (brine shrimp).pptxMAGOTI ERNEST
Although Artemia has been known to man for centuries, its use as a food for the culture of larval organisms apparently began only in the 1930s, when several investigators found that it made an excellent food for newly hatched fish larvae (Litvinenko et al., 2023). As aquaculture developed in the 1960s and ‘70s, the use of Artemia also became more widespread, due both to its convenience and to its nutritional value for larval organisms (Arenas-Pardo et al., 2024). The fact that Artemia dormant cysts can be stored for long periods in cans, and then used as an off-the-shelf food requiring only 24 h of incubation makes them the most convenient, least labor-intensive, live food available for aquaculture (Sorgeloos & Roubach, 2021). The nutritional value of Artemia, especially for marine organisms, is not constant, but varies both geographically and temporally. During the last decade, however, both the causes of Artemia nutritional variability and methods to improve poorquality Artemia have been identified (Loufi et al., 2024).
Brine shrimp (Artemia spp.) are used in marine aquaculture worldwide. Annually, more than 2,000 metric tons of dry cysts are used for cultivation of fish, crustacean, and shellfish larva. Brine shrimp are important to aquaculture because newly hatched brine shrimp nauplii (larvae) provide a food source for many fish fry (Mozanzadeh et al., 2021). Culture and harvesting of brine shrimp eggs represents another aspect of the aquaculture industry. Nauplii and metanauplii of Artemia, commonly known as brine shrimp, play a crucial role in aquaculture due to their nutritional value and suitability as live feed for many aquatic species, particularly in larval stages (Sorgeloos & Roubach, 2021).
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
2. ■ LUPUS : lupus erythematosus (LE) Its an autoimmune
inflammatory disease that includes a large spectrum of
manifestations, cutaneous manifestations include:
• Malar eminences in a “butterfly” distribution
INTRODUCTION
Interactions between susceptibility
genes and environmental factors result
in abnormal immune responses.
1. Activation of innate
immunity
2. reduced of immune
complexes and
apoptotic cells.
5. MATERIALS AND METHODS
Tissue sample of patients
Fresh skin tissues
• Patients with CLE, who received neither.
pharmaceutical nor physical therapies
n=11.
• Healthy controls n=4.
• Sera : tissue donors and other patients
n=34.
Murine Anti-DNA IgG
• Trypanosoma equiperdum derived
kinetoplast DNA = natural dsDNA
antigen that induces the generation of
Anti DNA IgG
• Anti DNA IgG clones = BALB c/mice
were immunized with a mixture of
kinetoplast DNA and incomplete
Freund’s adjuvant
Anti DNA IgG were labelled with
• Fluorescein isothiocynate (FITC)
• rhodamine
6. MATERIALS AND METHODS
Cell culturing: Murine PAM212 keratinocytes were grown in
Dulbecco's modified Eagle’s medium.
stimulation assays: cells starved in 2% fetal calf serum before the
addition :
• murine anti DNA , isotope control or recombinant mouseTWEAK (
inductor of apoptosis)
Apoptosis :The cells apoptosis was quantitated
using a caspasa tag caspase 3,7 Kit
7. MATERIALSAND METHODS
FLOW CYTOMETRY AND INMUNOFLUORESENCE
1.Technique that allows detecting a cell population in a sample in which other cell
samples predominate Information of each one of the cells analyzed and the
relationship that exits between the parameters of one cell and another.
2. Antibody-antigen reaction + fluorescent dye to the antibody, exposed to an
ultraviolet light microscope to be evidenced.
• PAM212 Cells + murine anti DNA IgG or control and FITC conjugated goat anti-
mouse IgG
• Cells + FITC or rhodamine anti DNA-IgG
Pretreated with DNase I
8. MATERIALSAND METHODS
Enzyme-linked immunosorbent assay
( ELISA )
It’s a serological laboratory test , in witch we can
identify an antibody or an antigen using (antibody or
antigen) absorber in a solid surface .
■ Identify the reaction between antigen- antibody
= conjugated antigamma globulin+ enzyme =
color
■ Color = amount of antibodies or antigent
• Affinities of anti-DNA IgG to ssDNA and ds DNA
• Blinding of collagen III and Ro52
WESTERN BLOT
It’s a laboratory method that uses antibodies to identify
proteins after the proteins have been separated by
electrophoresis in a gel according to the size.
• western blot to the proteins lysates ( cell culture)
• Cell lysates + control IgG (-)
• Cell lysates + anti SOCS1 IgG (+)
• Cell lysates + anti DNA IgG
• B-actin bands as a control
13. DISCUSSION
AUTHOR WHAT DIDTHEY SAID? YES/NO
• Y. Liang et al TWEAK/Fn14 signals mediate the
pathogenicity of anti-DNA IgG in CLE.
As a key regulator of cytokine
production, SOCS1 is dysregulated in
the damaged tissues of lupus er-
ythematosus.
YES
• Y. Xia et al They also found a specific
interaction between anti-DNA IgG
and other collagens, including
collagen IV and matrigel.
NO
• A. Reich et al
• T.D.Golan et al
UVB irradiation triggers
photosensitization and
exaggerates skin lesions in lupus
erythematosus through inducing
Ro52 expression.
YES
• O.J. Park et al Murine anti-DNA IgG increases
both Fn14 ex- pression and the
effect ofTWEAK on keratinocytes
by increasing apoptosis and
cytokine production.
YES
14. CONCLUSSIONS
1 Try to develop a way to decrease the effects of anti DNA IgG in patients with lupus, to
attenuate the symptoms.
2 Anti DNA IgG, could be a determinant factor at the moment of evaluate the clinic of the patient
with lupus, because a lot of manifestations are caused by it .
Anti-DNA IgG exists in skin lesions of lupus erythematosus and recognizes
novel antigens of collagen III and SOCS1, blinding to keratinocytes and induces
macrophage chemoattraction which may be related to the amplification of
TWEAK/Fn14 signals and the modulation of the SOCS1.