This document provides information about Norgen Biotek Corp., including their commitment to providing sample preparation kits and support services for nucleic acid purification, concentration, and stabilization for research and diagnostic applications. It specifically discusses their Rabies viral antigen detection kit, listing the kit catalog number, website for more information, and Norgen Biotek's corporate headquarters information and certifications. The document encourages contacting Norgen Biotek's technical support team with any questions.
This document provides information on several kits from Norgen Biotek Corp. for detecting HIV and related pathogens. It summarizes 3 kits that detect HIV: 1) an HIV quantitative RT-PCR kit that detects HIV RNA and has a reported linear range of 102-106 copies/μL, 2) an HIV proviral DNA PCR kit that detects HIV DNA with a linear range of 102-106 copies/μL, and 3) a plasma/serum HIV RT-PCR kit that isolates RNA from plasma/serum and detects HIV with a linear range of 10-8x106 VP/μL. It also summarizes kits for detecting Cryptococcus neoformans DNA and Pneumocystis jirove
This document describes a kit for detecting the human pathogen Mycobacterium tuberculosis using PCR. It discusses how the kit allows for isolation of DNA from samples using a spin column method. It then can detect M. tuberculosis through amplification of a region of its genome using provided master mixes and controls. The kit is designed for testing 24 samples at a time and provides high sensitivity and specificity for detection of this important pathogen.
SARS-CoV-2 (COVID-19) is the virus responsible for respiratory disease caused by a novel (new) coronavirus that was first detected in Wuhan City, Hubei Province, China, later causing a global pandemic.
The virus is contagious and can be spread from humans to humans primarily through the exchange of mucus droplets that are expelled through sneezes or coughs.
The SARS-CoV-2 is a severe acute respiratory syndrome coronavirus. This outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.
Real time rt-pcr Test molecolare per covid-19 (Sars-CoV-2) Protocollo UfficialeIULALaboratorioAnali
Protocollo ufficiale per la ricerca e amplificazione di RNA virale mediante tecnica PCR-RealTime per l'individuazione del virus Covid-19
Per maggiori informazioni: https://www.laboratorioiula.com
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics
RT2 Profiler PCR Arrays allow researchers to analyze gene expression profiles for focused panels of genes involved in biological pathways or disease states. The arrays provide laboratory-verified gene assays, integrated controls, and free data analysis software to generate a gene expression profile from a sample in less than 3 hours. Popular arrays analyze pathways such as extracellular matrix and adhesion molecules, WNT signaling, and cancer-related genes. The complete workflow begins with sample preparation and ends with data interpretation and publication of results.
Reporter assay and q pcr application 2012Elsa von Licy
This document summarizes a presentation on high-performance cell-based assay and qPCR technologies for pathway-focused research. The presentation overview discusses QIAGEN's SABiosciences portfolio, PCR arrays, Cignal and Cignal Lenti pathway reporters, and provides a summary. PCR arrays allow analysis of mRNA expression of up to 84 genes related to biological pathways in a single experiment. Cignal reporter assays use dual-luciferase reporters to study 45 signal transduction pathways. Cignal Lenti reporters use lentiviral delivery of luciferase or GFP reporters to study pathways in difficult to transfect cells like stem cells or primary cells.
Available now from Accuscience Ireland. Many topical and geographically important tests have been developed to allow producers and companion animal owners/veterinarians to screen animals quickly and with the high sensitivity of qPCR. Within the veterinary and agricultural range of pathogens detection kits are diseases and infections which can have significanteconomic impact. If your test is not available contact us to enquire about getting a custom kit made for you.
The Delta Seek detection kits utilise the sensitivity and speed of qPCR to get the most accurate data as quickly as possible. Each kit is specifically designed by our bioinformatics team to ensure the broadest possible detection profile and detection of all clinically relevant strains and subtypes. All test kits are validated in house on multiple qPCR platforms to ensure cross platform functionality.
This document provides information on several kits from Norgen Biotek Corp. for detecting HIV and related pathogens. It summarizes 3 kits that detect HIV: 1) an HIV quantitative RT-PCR kit that detects HIV RNA and has a reported linear range of 102-106 copies/μL, 2) an HIV proviral DNA PCR kit that detects HIV DNA with a linear range of 102-106 copies/μL, and 3) a plasma/serum HIV RT-PCR kit that isolates RNA from plasma/serum and detects HIV with a linear range of 10-8x106 VP/μL. It also summarizes kits for detecting Cryptococcus neoformans DNA and Pneumocystis jirove
This document describes a kit for detecting the human pathogen Mycobacterium tuberculosis using PCR. It discusses how the kit allows for isolation of DNA from samples using a spin column method. It then can detect M. tuberculosis through amplification of a region of its genome using provided master mixes and controls. The kit is designed for testing 24 samples at a time and provides high sensitivity and specificity for detection of this important pathogen.
SARS-CoV-2 (COVID-19) is the virus responsible for respiratory disease caused by a novel (new) coronavirus that was first detected in Wuhan City, Hubei Province, China, later causing a global pandemic.
The virus is contagious and can be spread from humans to humans primarily through the exchange of mucus droplets that are expelled through sneezes or coughs.
The SARS-CoV-2 is a severe acute respiratory syndrome coronavirus. This outbreak is an important reminder that the global community must strengthen national and international programs for detection and response to future disease outbreaks.
Real time rt-pcr Test molecolare per covid-19 (Sars-CoV-2) Protocollo UfficialeIULALaboratorioAnali
Protocollo ufficiale per la ricerca e amplificazione di RNA virale mediante tecnica PCR-RealTime per l'individuazione del virus Covid-19
Per maggiori informazioni: https://www.laboratorioiula.com
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics
RT2 Profiler PCR Arrays allow researchers to analyze gene expression profiles for focused panels of genes involved in biological pathways or disease states. The arrays provide laboratory-verified gene assays, integrated controls, and free data analysis software to generate a gene expression profile from a sample in less than 3 hours. Popular arrays analyze pathways such as extracellular matrix and adhesion molecules, WNT signaling, and cancer-related genes. The complete workflow begins with sample preparation and ends with data interpretation and publication of results.
Reporter assay and q pcr application 2012Elsa von Licy
This document summarizes a presentation on high-performance cell-based assay and qPCR technologies for pathway-focused research. The presentation overview discusses QIAGEN's SABiosciences portfolio, PCR arrays, Cignal and Cignal Lenti pathway reporters, and provides a summary. PCR arrays allow analysis of mRNA expression of up to 84 genes related to biological pathways in a single experiment. Cignal reporter assays use dual-luciferase reporters to study 45 signal transduction pathways. Cignal Lenti reporters use lentiviral delivery of luciferase or GFP reporters to study pathways in difficult to transfect cells like stem cells or primary cells.
Available now from Accuscience Ireland. Many topical and geographically important tests have been developed to allow producers and companion animal owners/veterinarians to screen animals quickly and with the high sensitivity of qPCR. Within the veterinary and agricultural range of pathogens detection kits are diseases and infections which can have significanteconomic impact. If your test is not available contact us to enquire about getting a custom kit made for you.
The Delta Seek detection kits utilise the sensitivity and speed of qPCR to get the most accurate data as quickly as possible. Each kit is specifically designed by our bioinformatics team to ensure the broadest possible detection profile and detection of all clinically relevant strains and subtypes. All test kits are validated in house on multiple qPCR platforms to ensure cross platform functionality.
The document provides information about Bayerpaul Group, a knowledge-based company founded in Iran in 2011 that produces vaccines, pharmaceuticals, and diagnostic test kits. It operates facilities for production, research and development, and quality control. The company has over 50 staff working in vaccine production, diagnostic kit production, antiviral and anticancer drug production, and quality control of pharmaceutical products. It provides details on its hepatitis B virus and cytomegalovirus real-time PCR kits for quantitative detection and their specifications.
Bayerpaul Group is a biotech company founded in 2011 in Tehran, Iran that produces vaccines, pharmaceuticals, and diagnostic test kits. It has four production lines for manufacturing viral vaccines, diagnostic kits, antiviral and anticancer drugs. It also has a quality control laboratory for testing products. The company began producing human influenza vaccines and diagnostic test kits in 2014.
This document provides an overview of RT2 Profiler PCR Arrays from SABiosciences, which allow for gene expression analysis from small samples and FFPE samples. The document discusses how PCR Arrays work using SABiosciences' PreAMP technology to increase sensitivity for samples containing as little as 1-100ng of RNA. It also reviews the performance data demonstrating the ability of PreAMP to detect more genes and shift genes with high Ct values into the detectable range. Finally, it highlights the complete PCR Array system from SABiosciences which provides optimized kits, controls, and software for reliable gene expression analysis from sample to results in about 3 hours.
neoplex pcr test kit [genematrix advantages of neo_plex covid-19 Rapid Test K...HK HuZef
NeoPlexTM COVID-19 has advantages over PowerChek 2019-nCoV for PCR-based detection of COVID-19 including higher sensitivity detecting down to 10-5 compared to 10-3 for PowerChek, targeting two genes (RdRp and N) specific to COVID-19 versus one broadly reactive gene, and including an internal process control for nucleic acid extraction and PCR. NeoPlex also offers advantages of being more user-friendly, requiring fewer PCR machines, allowing testing of more samples per kit, and having longer shelf life and reagent stability.
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...Simon Chung - genereach
A field-deployable RT-PCR system was found to perform equivalently to real-time RT-PCR in detecting type 2 porcine reproductive and respiratory syndrome virus (PRRSV). The field-deployable system provided results within 2 hours compared to 2.5 days for laboratory RT-PCR. Testing 50 vaccinated and 50 unvaccinated piglets over 11 weeks showed 96.25% agreement between the two methods. The field-deployable PCR system has potential for timely PRRSV detection and biosecurity management at points of need.
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics.
The document is a reference guide for pathway maps and related QIAGEN products. It includes a table of contents listing over 40 signaling pathways. For each pathway, it lists related RT2 Profiler PCR Arrays and Cignal Reporter Assay Kits from QIAGEN that can be used to study gene expression and transcription factor activity for that pathway. It also provides brief descriptions of the PCR array and reporter assay products and directs readers to QIAGEN websites for more information.
This document discusses using gene and miRNA expression profiling to develop biomarkers for monitoring genotoxicity. It describes:
1. Developing gene-based in vitro biomarkers for genotoxicity using RT2 Profiler PCR Arrays to analyze expression of DNA damage and p53 pathway genes in HepG2 cells exposed to genotoxic and non-genotoxic compounds. 11 genes were identified as classifiers.
2. Identifying miRNA-based in vivo biomarkers by profiling miRNA expression in mouse liver after exposure to the carcinogen ENU using miScript PCR Arrays. The mir-34 family showed temporal changes and clustering analysis identified differentially expressed miRNAs.
3. miRNA profiles have potential to serve as biomarkers for genotoxicity
10 Tips to maximize your Real Time PCR Success - Download the Technical NoteQIAGEN
This document provides 10 tips for maximizing success with real-time PCR. The tips include using high-quality nucleic acid templates, determining template concentration and purity, checking storage conditions, using the optimal template amount, determining reaction efficiency for each primer pair, properly storing primers and probes, preventing contamination, thoroughly mixing reaction components, performing necessary control reactions, and double checking cycler settings.
This document provides information about Norgen Biotek's cell-free circulating DNA and RNA purification kits. It summarizes their product lines for isolating cell-free circulating DNA and RNA from various bodily fluids like plasma, serum, and urine. The kits are designed to isolate all sizes of circulating DNA and RNA and provide purified samples compatible with downstream applications like PCR and NGS. Specifications for different kit sizes are provided based on input sample volume and elution volume.
Quality Control of RNA Samples - For Gene-Expression Results you Can Rely onQIAGEN
By their very nature RNA molecules, especially mRNA and regulator RNA, are labile and can be highly unstable and sensitive to heat, UV and RNase contamination. The quality, relevance and scientific impact of gene expression results directly depends on the ability to extract RNA without losing any fraction of interest, while preserving the integrity of the biological information it carries. RNA quality control is thus critical to ensure high-quality results and for turning these results into actionable insights with confidence.
In this webinar, we will introduce you to the main parameters influencing RNA-based assays and their respective impact on downstream applications, discuss how to monitor them and cover the advantages of automation for quality control along complex workflows.
RT2 Profiler PCR Arrays are a real-time PCR technology that allows researchers to study gene expression patterns across biological pathways and processes. The arrays contain pre-designed primer assays for 84 relevant genes as well as controls on a single plate in a 96-well format. The gene content of the arrays is selected based on biological relevance and published associations with relevant pathways. The primer assays on the arrays undergo extensive validation for sensitivity, specificity, reproducibility, and amplification efficiency. The PCR Array system also includes optimized components for RNA isolation, cDNA synthesis, and real-time PCR to provide a complete validated workflow for gene expression analysis from sample to results.
This document describes pathway-powered PCR arrays for gene expression analysis. It discusses:
- PCR arrays that analyze the expression of genes involved in biological pathways, covering sample prep through data analysis.
- Over 140 pathway-focused PCR arrays are available covering cancer, inflammation, neuroscience, and other areas.
- The PCR arrays allow analysis of mRNA expression from various sample types in a high-sensitivity and reproducible manner using real-time PCR instrumentation.
PCR Array Data Analysis Tutorial: qPCR Technology Webinar Series Part 3QIAGEN
This webinar presentation provides an overview and tutorial on analyzing data from RT2 Profiler PCR Array experiments. It discusses organizing raw Ct value data, performing ΔCt and ΔΔCt calculations to analyze gene expression changes between sample groups, and using the GeneGlobe Data Analysis Center web portal to analyze the data. The webinar highlights new features of the Data Analysis Center including improved data visualization and an upgraded sample manager. It emphasizes following the standard protocol for setting baselines and thresholds when analyzing PCR array data.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Neil leblanc f7 rapid methods call amsterdam may 2010sva-slu_oie-cc
The document summarizes a strategic meeting discussing the development and use of multiplex assays using Luminex technology. Methods allow the detection of up to 100 unique assays in a single sample, and have been used to detect and subtype pestiviruses, avian influenza viruses, African swine fever virus, and cytokines. The technology provides a sensitive and specific platform for pathogen identification and characterization applicable to diagnostic laboratories.
The document discusses BioChain's products for PCR and sample preparation, including PCR enzymes, reverse transcriptases, master mixes, dNTPs, and supporting reagents. It provides details on BioChain's Taq DNA polymerase and Hot Start Taq DNA polymerase, which are produced under strict quality control. It also describes BioChain's UltraScript reverse transcriptase, which is ideal for cDNA synthesis of templates with secondary structure or high GC content. Furthermore, it mentions BioChain's pre-mixed master mixes for standard and quantitative PCR, which offer convenience and reproducibility.
Microbial S.L. is a biotechnological company devoted to the design and production of products for the rapid detection of pathogen microorganisms in environmental and food samples.
This document discusses avian influenza (H5N1) detection and analysis using real-time PCR. It describes the influenza virus, including its structure and types. Avian influenza is highly contagious in birds and can be fatal. Diagnosis involves virus isolation, serology tests, and molecular tests like RT-PCR and real-time RT-PCR. Real-time PCR allows for amplification and detection of targeted DNA sequences in one step and provides amplification curves and results in about 3 hours. The workflow described screens samples for influenza A using a matrix PCR, then checks positive samples for H5 and N1 subtypes using multiplex real-time PCR on the LightCycler system.
The document provides an overview of PCR array technology for gene expression analysis from QIAGEN. It describes how RT2 Profiler PCR arrays work to simultaneously analyze the expression of multiple pathway-focused genes. Popular array types are listed, including inflammatory cytokines and receptors, oxidative stress, and stem cell arrays. The document highlights the simplicity, performance, and relevance of PCR arrays for expression profiling. It also provides examples of array applications in angiogenesis, cancer biomarker discovery, immune response, and determining drug toxicity.
This document defines key terms related to disease transmission and the immune system. It explains that pathogens can transmit diseases through direct or indirect contact. The body has mechanical, chemical, and cellular defenses against pathogens, including white blood cells that distinguish self from non-self and produce antibodies. Vaccination exposes the body to harmless antigens to trigger antibody production and develop immunological memory for long-term protection. Both active and passive immunity are described, with active immunity resulting from infection or vaccination and producing memory cells, while passive immunity involves acquiring antibodies without memory cell development.
This document defines pathogens as microorganisms that cause disease, including bacteria, viruses, fungi and protozoa. It explains that to be a pathogen, an organism must gain entry to the host, colonize tissues, resist defenses, and damage tissues. It discusses how pathogens enter through the respiratory, digestive, or skin systems and describes some of the body's defenses against pathogens like mucus barriers and stomach acid. Finally, it notes pathogens can cause disease by damaging tissues or producing toxins.
The document provides information about Bayerpaul Group, a knowledge-based company founded in Iran in 2011 that produces vaccines, pharmaceuticals, and diagnostic test kits. It operates facilities for production, research and development, and quality control. The company has over 50 staff working in vaccine production, diagnostic kit production, antiviral and anticancer drug production, and quality control of pharmaceutical products. It provides details on its hepatitis B virus and cytomegalovirus real-time PCR kits for quantitative detection and their specifications.
Bayerpaul Group is a biotech company founded in 2011 in Tehran, Iran that produces vaccines, pharmaceuticals, and diagnostic test kits. It has four production lines for manufacturing viral vaccines, diagnostic kits, antiviral and anticancer drugs. It also has a quality control laboratory for testing products. The company began producing human influenza vaccines and diagnostic test kits in 2014.
This document provides an overview of RT2 Profiler PCR Arrays from SABiosciences, which allow for gene expression analysis from small samples and FFPE samples. The document discusses how PCR Arrays work using SABiosciences' PreAMP technology to increase sensitivity for samples containing as little as 1-100ng of RNA. It also reviews the performance data demonstrating the ability of PreAMP to detect more genes and shift genes with high Ct values into the detectable range. Finally, it highlights the complete PCR Array system from SABiosciences which provides optimized kits, controls, and software for reliable gene expression analysis from sample to results in about 3 hours.
neoplex pcr test kit [genematrix advantages of neo_plex covid-19 Rapid Test K...HK HuZef
NeoPlexTM COVID-19 has advantages over PowerChek 2019-nCoV for PCR-based detection of COVID-19 including higher sensitivity detecting down to 10-5 compared to 10-3 for PowerChek, targeting two genes (RdRp and N) specific to COVID-19 versus one broadly reactive gene, and including an internal process control for nucleic acid extraction and PCR. NeoPlex also offers advantages of being more user-friendly, requiring fewer PCR machines, allowing testing of more samples per kit, and having longer shelf life and reagent stability.
A field-deployable RT-PCR system performs equivalently to real-time RT-PCR in...Simon Chung - genereach
A field-deployable RT-PCR system was found to perform equivalently to real-time RT-PCR in detecting type 2 porcine reproductive and respiratory syndrome virus (PRRSV). The field-deployable system provided results within 2 hours compared to 2.5 days for laboratory RT-PCR. Testing 50 vaccinated and 50 unvaccinated piglets over 11 weeks showed 96.25% agreement between the two methods. The field-deployable PCR system has potential for timely PRRSV detection and biosecurity management at points of need.
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail. PCR was invented in 1984 by the American biochemist Kary Mullis at Cetus Corporation. It is fundamental to much of genetic testing including analysis of ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics.
The document is a reference guide for pathway maps and related QIAGEN products. It includes a table of contents listing over 40 signaling pathways. For each pathway, it lists related RT2 Profiler PCR Arrays and Cignal Reporter Assay Kits from QIAGEN that can be used to study gene expression and transcription factor activity for that pathway. It also provides brief descriptions of the PCR array and reporter assay products and directs readers to QIAGEN websites for more information.
This document discusses using gene and miRNA expression profiling to develop biomarkers for monitoring genotoxicity. It describes:
1. Developing gene-based in vitro biomarkers for genotoxicity using RT2 Profiler PCR Arrays to analyze expression of DNA damage and p53 pathway genes in HepG2 cells exposed to genotoxic and non-genotoxic compounds. 11 genes were identified as classifiers.
2. Identifying miRNA-based in vivo biomarkers by profiling miRNA expression in mouse liver after exposure to the carcinogen ENU using miScript PCR Arrays. The mir-34 family showed temporal changes and clustering analysis identified differentially expressed miRNAs.
3. miRNA profiles have potential to serve as biomarkers for genotoxicity
10 Tips to maximize your Real Time PCR Success - Download the Technical NoteQIAGEN
This document provides 10 tips for maximizing success with real-time PCR. The tips include using high-quality nucleic acid templates, determining template concentration and purity, checking storage conditions, using the optimal template amount, determining reaction efficiency for each primer pair, properly storing primers and probes, preventing contamination, thoroughly mixing reaction components, performing necessary control reactions, and double checking cycler settings.
This document provides information about Norgen Biotek's cell-free circulating DNA and RNA purification kits. It summarizes their product lines for isolating cell-free circulating DNA and RNA from various bodily fluids like plasma, serum, and urine. The kits are designed to isolate all sizes of circulating DNA and RNA and provide purified samples compatible with downstream applications like PCR and NGS. Specifications for different kit sizes are provided based on input sample volume and elution volume.
Quality Control of RNA Samples - For Gene-Expression Results you Can Rely onQIAGEN
By their very nature RNA molecules, especially mRNA and regulator RNA, are labile and can be highly unstable and sensitive to heat, UV and RNase contamination. The quality, relevance and scientific impact of gene expression results directly depends on the ability to extract RNA without losing any fraction of interest, while preserving the integrity of the biological information it carries. RNA quality control is thus critical to ensure high-quality results and for turning these results into actionable insights with confidence.
In this webinar, we will introduce you to the main parameters influencing RNA-based assays and their respective impact on downstream applications, discuss how to monitor them and cover the advantages of automation for quality control along complex workflows.
RT2 Profiler PCR Arrays are a real-time PCR technology that allows researchers to study gene expression patterns across biological pathways and processes. The arrays contain pre-designed primer assays for 84 relevant genes as well as controls on a single plate in a 96-well format. The gene content of the arrays is selected based on biological relevance and published associations with relevant pathways. The primer assays on the arrays undergo extensive validation for sensitivity, specificity, reproducibility, and amplification efficiency. The PCR Array system also includes optimized components for RNA isolation, cDNA synthesis, and real-time PCR to provide a complete validated workflow for gene expression analysis from sample to results.
This document describes pathway-powered PCR arrays for gene expression analysis. It discusses:
- PCR arrays that analyze the expression of genes involved in biological pathways, covering sample prep through data analysis.
- Over 140 pathway-focused PCR arrays are available covering cancer, inflammation, neuroscience, and other areas.
- The PCR arrays allow analysis of mRNA expression from various sample types in a high-sensitivity and reproducible manner using real-time PCR instrumentation.
PCR Array Data Analysis Tutorial: qPCR Technology Webinar Series Part 3QIAGEN
This webinar presentation provides an overview and tutorial on analyzing data from RT2 Profiler PCR Array experiments. It discusses organizing raw Ct value data, performing ΔCt and ΔΔCt calculations to analyze gene expression changes between sample groups, and using the GeneGlobe Data Analysis Center web portal to analyze the data. The webinar highlights new features of the Data Analysis Center including improved data visualization and an upgraded sample manager. It emphasizes following the standard protocol for setting baselines and thresholds when analyzing PCR array data.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Neil leblanc f7 rapid methods call amsterdam may 2010sva-slu_oie-cc
The document summarizes a strategic meeting discussing the development and use of multiplex assays using Luminex technology. Methods allow the detection of up to 100 unique assays in a single sample, and have been used to detect and subtype pestiviruses, avian influenza viruses, African swine fever virus, and cytokines. The technology provides a sensitive and specific platform for pathogen identification and characterization applicable to diagnostic laboratories.
The document discusses BioChain's products for PCR and sample preparation, including PCR enzymes, reverse transcriptases, master mixes, dNTPs, and supporting reagents. It provides details on BioChain's Taq DNA polymerase and Hot Start Taq DNA polymerase, which are produced under strict quality control. It also describes BioChain's UltraScript reverse transcriptase, which is ideal for cDNA synthesis of templates with secondary structure or high GC content. Furthermore, it mentions BioChain's pre-mixed master mixes for standard and quantitative PCR, which offer convenience and reproducibility.
Microbial S.L. is a biotechnological company devoted to the design and production of products for the rapid detection of pathogen microorganisms in environmental and food samples.
This document discusses avian influenza (H5N1) detection and analysis using real-time PCR. It describes the influenza virus, including its structure and types. Avian influenza is highly contagious in birds and can be fatal. Diagnosis involves virus isolation, serology tests, and molecular tests like RT-PCR and real-time RT-PCR. Real-time PCR allows for amplification and detection of targeted DNA sequences in one step and provides amplification curves and results in about 3 hours. The workflow described screens samples for influenza A using a matrix PCR, then checks positive samples for H5 and N1 subtypes using multiplex real-time PCR on the LightCycler system.
The document provides an overview of PCR array technology for gene expression analysis from QIAGEN. It describes how RT2 Profiler PCR arrays work to simultaneously analyze the expression of multiple pathway-focused genes. Popular array types are listed, including inflammatory cytokines and receptors, oxidative stress, and stem cell arrays. The document highlights the simplicity, performance, and relevance of PCR arrays for expression profiling. It also provides examples of array applications in angiogenesis, cancer biomarker discovery, immune response, and determining drug toxicity.
This document defines key terms related to disease transmission and the immune system. It explains that pathogens can transmit diseases through direct or indirect contact. The body has mechanical, chemical, and cellular defenses against pathogens, including white blood cells that distinguish self from non-self and produce antibodies. Vaccination exposes the body to harmless antigens to trigger antibody production and develop immunological memory for long-term protection. Both active and passive immunity are described, with active immunity resulting from infection or vaccination and producing memory cells, while passive immunity involves acquiring antibodies without memory cell development.
This document defines pathogens as microorganisms that cause disease, including bacteria, viruses, fungi and protozoa. It explains that to be a pathogen, an organism must gain entry to the host, colonize tissues, resist defenses, and damage tissues. It discusses how pathogens enter through the respiratory, digestive, or skin systems and describes some of the body's defenses against pathogens like mucus barriers and stomach acid. Finally, it notes pathogens can cause disease by damaging tissues or producing toxins.
This document discusses pathogens and the history of infection control. It defines pathogens as microorganisms like bacteria and viruses that can cause disease. It describes how bacteria and viruses infect the body and make us feel ill. The document then discusses important figures in the history of infection control like Ignaz Semmelweis, who reduced childbed fever deaths by insisting doctors wash their hands with chlorine water before examining patients, and Australian scientists Marshall and Warren, who discovered the bacteria Heliobacter pylori causes stomach ulcers.
This document defines viruses and summarizes their key characteristics and classification. It describes how viruses were first discovered through experiments filtering bacteria and plant extracts. Viruses are non-cellular particles that contain genetic material and invade living cells. They are smaller than bacteria, contain either DNA or RNA, and lack organelles. Viruses replicate only inside host cells and do not undergo binary fission. They have various structures depending on their nucleic acid arrangement and symmetry. Viruses are classified into groups based on their nucleic acids and ability to produce mRNA.
Viruses are non-living particles that can only reproduce inside host cells. They are smaller than bacteria and contain genetic material surrounded by a protein coat. Viruses come in various shapes and sizes and cause diseases like influenza, measles, HIV/AIDS, and some cancers. Edward Jenner developed the first vaccine for smallpox using a related cowpox virus. Viruses are identified based on their morphology, genetic material, presence of an envelope, capsid shape, host cell, and more. They exist in nature as parasites and depend on host cells for reproduction through lysogenic or lytic cycles.
This document summarizes different types of pathogens including bacteria, viruses, fungi, protozoa, parasitic worms, and prions. It defines pathogens and reservoirs, and describes how pathogens are transmitted directly from person to person or indirectly through vectors like mosquitoes or ticks. Examples are provided of diseases caused by different pathogens and their symptoms, including pneumonia from bacteria, the common cold from viruses, athlete's foot from fungi, malaria from protozoa, intestinal infections from parasitic worms, and mad cow disease from prions.
This document provides information about a plant pathogen detection kit from Norgen Biotek Corp. that allows for the isolation and detection of fungal pathogens from plant samples using PCR. The kit contains components for isolating DNA from plant tissues using spin column chromatography. It also contains master mixes for amplifying fungal DNA, as well as controls. The kit is a ready-to-use system for detecting pathogens like Aspergillus niger, Botrytis cinerea, Cladosporium cladosporioides, Penicillium sp., and others from plant samples in under 3 hours.
This document describes Norgen Biotek Corporation, a company that provides sample preparation kits for RNA, microRNA, DNA and protein purification from various sample types. It offers over 40 kits for total RNA isolation without the use of phenol, including kits for cultured cells, tissues, blood, and formalin-fixed paraffin-embedded samples. The document highlights that Norgen's kits recover a full size range of RNA, including small RNA and microRNA, and provide high quality RNA suitable for various downstream applications. It also provides contact information, ordering details, and an overview of Norgen's RNA purification kits and their applications.
This document describes products for preserving and purifying RNA from blood samples, including:
1. A complete system for collecting whole blood in RNA preservative tubes, stabilizing RNA for up to 12 days at room temperature, and purifying RNA using spin column chromatography.
2. RNA preservative tubes that stabilize blood RNA for up to 12 days at room temperature or 14 days at 2-8°C.
3. RNA purification kits compatible with Norgen, Tempus, and PAXgene blood RNA preservative tubes to isolate RNA preserved in these tubes.
The system and products aim to efficiently preserve blood RNA quality and allow downstream analysis of gene expression and sequencing applications.
Foregene presentation-better life scienceMaggie Ma
This is Maggie from Foregene(www.foreivd.com), a life science company focused on molecular biology.
We're founded by experts from Yale university, and Gene company with 20+ years experience in life science industry, and with 10+ years foundation in life science reagent, including DNA/RNA isolation kit, PCR/qPCR reagent, genotyping kit, IVD kit, PCR machines, and other consumables, etc.
In 2019, we launched out the covid-19 RT-PCR kit in 3 days at the pandemic break, and ranked the 1st producer for the test kits in Southwestern China.Besides, the life science reagent is also covered in 80%+ universities in China, and the large hospital labs.
Any questions, feel free to contact me.
Regards,
Maggie | Sales
Foregene Co., Ltd
www.foreivd.com
Mob:008615281067355
This document describes a magnetic bead-based kit for isolating DNA from preserved saliva samples. The kit provides a rapid method for purifying high-quality, inhibitor-free genomic DNA from saliva samples in as little as 30 minutes. Comparisons between the magnetic bead kit and Norgen's column-based saliva DNA isolation kit show the two methods produce DNA of similar integrity, yield, and concentration, demonstrating the consistent performance of the magnetic bead system. The purified DNA is suitable for downstream applications such as PCR, sequencing, and microarrays.
Technical Guide to Qiagen PCR Arrays - Download the GuideQIAGEN
Total RNA discovery with RT2 and miScript PCR Arrays : Explore the RNA universe - Whatever your destination within the RNA universe, QIAGEN will help you get there. The miRNeasy kits deliver pure, high-quality total RNA from a broad range of samples. The RT2 and miScript PCR arrays are a complete solution both for focused analysis of gene and microRNA expression and for validation of microarray and RNA sequencing experiments. Together with the powerful analytics tools of GeneGlobe® and QIAGEN Ingenuity® Pathway Analysis, these products give you a smooth path from your sample to high-quality results.
A Field-Deployable Insulated Isothermal PCR-Based System for Rapid and Sensit...Simon Chung - genereach
POCKIT Central PCR System for Detecting African Swine Fever Virus in Vietnam
The document describes a study evaluating the POCKIT Central PCR system for detecting African Swine Fever Virus (ASFV) in Vietnam. The system provides fully automated sample-to-answer detection of ASFV in 85 minutes using cartridges that integrate nucleic acid extraction and PCR. The study found the POCKIT Central system performed equivalently to the OIE reference real-time PCR method, with high analytical sensitivity and specificity. The automated system reduces hands-on time and human error compared to traditional PCR methods. It provides a simple workflow for rapid on-site detection of ASFV to help control the ongoing spread of the disease
Norgen Biotek is dedicated to providing sample preparation kits for exosome purification, RNA isolation, and nucleic acid stabilization from bodily fluids for research and diagnostic applications. They offer a comprehensive selection of kits for intact exosome purification and RNA isolation from plasma, serum, urine, cell culture media, and other samples. Their kits allow purification of exosomes ranging in size from 40-150 nm without the need for ultracentrifugation or other specialized equipment.
RT2 Profiler PCR Arrays allow researchers to analyze gene expression in biological pathways or disease states using real-time PCR. The document discusses:
1. PCR Arrays focus on profiling genes relevant to specific pathways or disease states. They provide a simple, reproducible, and sensitive way to simultaneously profile expression of many genes related to a pathway.
2. Examples are provided demonstrating how PCR Arrays have been used in cancer research to discover breast cancer biomarkers, in immunology research to monitor cytokine induction, and in toxicity research to determine drug toxicity profiles.
3. Key advantages of PCR Arrays are highlighted, including their simplicity, performance, relevance to specific pathways, and ability to analyze gene expression from small amounts
Some sample sources present special challenges in RNA isolation or contain substances that cause problems in RNA analysis. These guides to RNA isolation have tips for a whole range of sample types, including guidance on the best kits for each.
Polymerase chain reaction (PCR) is a nucleic acid amplification technique used in diagnostic microbiology to rapidly detect pathogens. PCR works by thermally cycling DNA to exponentially amplify target sequences using DNA polymerase. It is highly sensitive and specific, allowing detection of pathogens that cannot be cultured. Real-time PCR further allows quantification by measuring amplification over cycles. PCR has revolutionized infectious disease diagnosis by enabling rapid, sensitive detection of various microbes from clinical samples.
This document describes Norgen Biotek's Plant/Fungi Total RNA Purification Kit, which provides a rapid method for isolating total RNA, including microRNA, from plant and fungal samples. The kit uses a spin column format to purify RNA from fresh or frozen tissues without phenol/chloroform extractions. It isolates all sizes of RNA, from mRNA to microRNA. The purified RNA is of high quality and quantity and can be used in downstream applications such as PCR and expression arrays. Norgen also offers the kit in a 96-well format for high-throughput purification of plant/fungi RNA.
Simultaneous Isolation of RNA & DNA from one FFPE SampleQIAGEN
The document summarizes the AllPrep DNA/RNA FFPE Kit, which simultaneously isolates genomic DNA and total RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples. FFPE samples are lysed and centrifuged to separate the RNA-containing supernatant from the DNA-containing pellet. The supernatant and pellet are then processed separately to purify high-quality RNA and DNA suitable for downstream applications like real-time PCR and pyrosequencing. Results show the kit yields RNA and DNA of sufficient quality and quantity from old FFPE samples for gene expression analysis and detection of genetic mutations.
PCR is a technique used to amplify DNA sequences. It was invented in 1983 by Kary Mullis. Some key benefits of PCR include its high accuracy, speed, and applications in disease diagnosis, drug development, genetic studies, and forensics. The PCR process involves denaturing DNA, annealing primers, and extending the DNA strands in thermal cycling. Results are analyzed using gel electrophoresis or real-time PCR to detect amplified DNA sequences. PCR has many applications and can be used with various sample types in fields like medicine, environmental science, agriculture, and forensics.
This document provides instructions for using the Novel Coronavirus (2019-nCoV) Nucleic Acid Diagnostic Kit (PCR-Fluorescence Probing) for the qualitative detection of SARS-CoV-2 in respiratory specimens. The kit uses real-time RT-PCR to detect SARS-CoV-2 RNA and includes positive and negative controls. Test results along with clinical observations are needed for accurate patient diagnosis and management. Strict laboratory procedures and biosafety precautions must be followed when handling specimens and performing the test.
Sensitivity assay of polymerase chain reaction for detection of canine adeno ...Alexander Decker
This study aimed to determine the minimum detection limit of canine adeno virus (CAV) in clinical samples using polymerase chain reaction (PCR). PCR was performed on 40 blood samples from different regions of India using reported primers for CAV detection. Positive PCR samples were serially diluted and used as templates to determine the lowest concentration detectable by PCR. The minimum detection limit was found to be a 1:1000 dilution, equivalent to 0.20 ng of DNA per microliter. The study demonstrated PCR is a sensitive method for early detection of CAV infection in clinical samples.
qBiomarker Somatic Mutation PCR Arrays are panels of real-time PCR assays that allow for sensitive detection of mutations in 85-370 genes from fresh or FFPE samples. They provide detection of cancer-associated mutations with superior sensitivity compared to other methods using a simple real-time PCR protocol. The document describes the workflow which involves extracting DNA from samples, mixing with mastermix, distributing across the PCR array plate, running on a real-time PCR instrument, and analyzing data to make mutation calls. Examples of available arrays are provided that focus on different cancer types and pathways.
A next generation sequencing based sample-to-result pharmacogenomics research...Thermo Fisher Scientific
Pharmacogenomics (PGx) is the study of genetic variations in terms of their response to drugs. Variations in gene sequence or copy numbers may result in complete loss of function, partial decrease or increase in enzyme activity, or an altered affinity for substrates, which may in turn significantly impact drug efficacy. PGx studies are becoming increasingly important for precision medicine. We have developed a next generation sequencing (NGS) PGx research solution with increased flexibility on the assay targets and combined detection of SNP/INDEL genotyping and CNV using Ion AmpliSeq™ technology for low to medium throughput laboratories. With this highly multiplexed PGx research panel we can profile a set of 136 genetic markers in 40 known PGx related genes (Table 1) and determine CYP2D6 copy number variation (CNV, Figure 1) in a single reaction using Ion Torrent™ semiconductor sequencing.
Molecular methods of diagnosing infectious diseaseaka_sam15
Molecular methods such as PCR and LAMP have revolutionized infectious disease diagnosis by allowing rapid and sensitive detection of pathogens. PCR amplifies specific DNA sequences, and real-time PCR with fluorescent probes like TaqMan or molecular beacons allows quantification during amplification. LAMP is an inexpensive isothermal method that amplifies DNA with high sensitivity and specificity using multiple primers and strand displacement. Both PCR and LAMP have advanced diagnosis by detecting pathogens earlier and multiplexing the detection of multiple targets in a single sample.
The BenchSmart 96 is a semi-automated pipettor with interchangeable heads that provides a volume range of 0.5 μL to 1000 μL. It has a touchscreen interface that controls pipetting functions and modes. The pipettor offers versatility through three interchangeable heads, high reliability and repeatability for experiments, and intuitive software that makes programming and workflows more efficient.
1. Norgen Biotek's cf-DNA preservative tubes preserve cell-free DNA (cf-DNA) in whole blood samples for up to 30 days at ambient temperatures without using formaldehyde.
2. The tubes maximize plasma volume recovery of 6-7mL even after shipping and prevent hemolysis and apoptosis of blood cells that could degrade cf-DNA quality.
3. cf-DNA and genomic DNA remain stable in the preservative tubes for 30 days at room temperature and 8 days at 37°C, allowing preserved samples to be shipped or stored before downstream analysis of cf-DNA.
Norgen Biotek offers customizable saliva DNA collection kits and services. They provide branded packaging, documentation, shipping solutions, and expert support to create customized workflows for clients. With options for customizing packaging, labels, and instructions, Norgen Biotek aims to optimize DNA collection kits while helping clients build their own brand recognition. Norgen Biotek pledges speedy delivery and same-day technical support to integrate their products into clients' operations.
This document summarizes a saliva DNA isolation kit from Norgen Biotek Corp. that uses magnetic bead technology. It can isolate high-quality DNA from both fresh and preserved saliva samples. The isolation process involves a proteinase K incubation followed by binding of DNA to magnetic beads while removing proteins. This results in high yields of intact genomic DNA suitable for downstream applications like PCR, microarrays, sequencing, and genotyping.
This document describes a cell-free circulating DNA and RNA isolation kit that can isolate nucleic acids from small sample volumes of plasma, serum, urine, and follicular fluid. The kit offers scalable input volumes from 0.1 mL to 30 mL, provides rapid, robust and reliable isolation with excellent linearity, and produces inhibitor-free nucleic acids that are suitable for all downstream applications including fetal DNA screening, microarrays, genotyping, NGS, biomarker discovery, and methylation/epigenetics studies.
This document describes RNA purification kits from Norgen Biotek for stem cell research. It provides information on several kits for purifying total RNA, RNA/DNA, RNA/proteins, and other biomolecules from small sample inputs including single cells. The kits allow sensitive purification of all RNA sizes including microRNA. They provide high quality RNA suitable for downstream applications such as qRT-PCR and NGS. Several figures show examples of high yields and quality of RNA purified from small tissue samples and cell numbers down to single cells.
The document discusses blood sample preparation kits from Norgen Biotek for isolating genomic DNA and RNA from blood. It provides details on their single-column DNA and RNA isolation kits for various sample sizes ranging from 1 μL to 10 mL of whole blood. The kits allow for rapid isolation of high-quality nucleic acids within 30-50 minutes that are suitable for downstream applications like PCR, sequencing, and microarrays. Product specifications, features, benefits, and applications are described for different kit catalog numbers.
3. Contact our Technical Support Team between the hours of 9:00 and 5:30 EST
(Eastern Standard Time) at (905) 227-8848 or Toll Free in North America at
1-866-667-4362.
Technical support can also be obtained from our website (www.norgenbiotek.com)
or through email at techsupport@norgenbiotek.com.
Technical Support
Ordering Information
Telephone Orders
Customer service representatives are available to receive orders Monday through
Friday from 9:00 A.M. to 5:30 P.M. EST (Eastern Standard Time).
When placing an order, please be prepared to provide us with the following infor-
mation:
1. Purchase order number
2. Customer number (if known)
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4. Shipping Address
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7. Product catalogue number, description, size and quantity
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We also accept orders by email, fax and mail. When placing an order by email, fax
or mail, please ensure that the information listed above is included in order to
expedite ordering.
To Order by Phone:
Telephone: (905) 227-8848
Toll Free in North America: 1-866-667-4362
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Norgen Biotek Corp.
3430 Schmon Parkway
Thorold, ON
L2V 4Y6
CANADA
3
4. ANIMAL PATHOGEN DETECTION
Principle of the Test
Norgen’s PCR Detection Kits constituent a ready-to-use system for the isolation and detection
of animal pathogens using end-point or one-step RT-PCR. The kits first allows for the isolation of
RNA or DNA from samples using spin-column chromatography based on Norgen’s proprietary
resin. The RNA or DNA is isolated free from inhibitors, and can then be used as the template in
a one step RT-PCR reaction for pathogen detection using the provided Detection Mastermix.
The Detection Mastermix contains reagents and enzymes for the specific amplification of a
region of the viral genome.
In addition, Norgen’s PCR Detection Kits contain a second Mastermix, the RT-PCR or PCR
Control Master Mix, which can be used to identify possible PCR inhibition and/or inadequate
isolation via a separate RT-PCR reaction with the use of the provided PCR control (PCRC) or
Isolation Control (IsoC), respectively. The kits are designed to allow for the testing of 24
samples.
Key Features
Rapid isolation of high quality RNA or DNA from blood, plasma/serum, swabs, or viral
culture, urine, nasal exudates or swabs
High sensitivity and specificity
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
End-Point PCR Real-Time PCR
102
103
104
101
NC
Target
M
FCV
M NC
Target
M
FCV
M
4
AnimalPathogenDetection
All kits are available in two formats: End-Point PCR or Real-Time PCR
5. 5
AnimalPathogenDetection
Product Description Cat. # Page
Feline Calicivirus
RT-PCR Detection Kit
Isolation and detection of Feline Calicivirus from
blood and swab samples using end-point one-step
RT-PCR.
43900 6
Feline Leukemia Virus
RT-PCR Detection Kit
Isolation and detection of Feline Leukemia Virus
from blood and swab samples using end-point
one-step RT-PCR.
44000 7
Feline Immunodeficiency
Virus RT-PCR Kit
Isolation and detection of Feline Immunodeficiency
Virus from blood samples using end-point one-step
RT-PCR.
44100 8
West Nile Virus
RT-PCR Detection Kit
Isolation and detection of West Nile Virus from
blood or plasma samples using end-point one-step
RT-PCR.
44200 9
Feline Herpes Virus
PCR Detection Kit
Isolation and detection of Feline Herpes Virus from
plasma/serum samples, swabs or viral culture using
end-point PCR.
44300 10
Feline Infectious Peritonitis Virus
RT-PCR Detection Kit
Isolation and detection of Feline Infectious
Peritonitis Virus (FIPV) from blood samples using
end-point one-step RT-PCR.
44400 11
Dirofilaria immitis
PCR Detection Kit
Isolation and detection of Dirofilaria immitis from
blood samples using end-point PCR.
44500 12
Leptospira interrogans
PCR Detection Kit
Isolation and detection of Leptospira interrogans
from urine samples using end-point PCR.
44600 13
Toxoplasma gondii
PCR Detection Kit
Isolation and detection of Toxoplasma gondii from
blood samples using end-point PCR.
44700 14
Bordetella bronchiseptica
PCR Detection Kit
Isolation and detection of Bordetella
bronchiseptica from nasal exudates or pharyngeal
swabs using end-point PCR.
44900 15
Canine Parvovirus
PCR Detection Kit
Isolation and detection of Canine Parvovirus from
blood samples using end-point PCR.
45000 16
Feline Panleukopenia Virus
PCR Detection Kit
Isolation and detection of Feline Panleukopenia
Virus from blood samples using end-point PCR.
45100 17
Borrelia burgdorferi
PCR Detection Kit
Isolation and detection of Borrelia burgdorferi from
urine samples using end-point PCR
45200 18
Rabies RT-PCR Detection Kit
Isolation and detection of Rabies from blood
samples using end-point one-step RT-PCR.
45300 19
TABLE OF CONTENTS
6. Figure 1. Sensitivity of Detection using the Feline Calicivirus RT-
PCR Detection Kit. A representative 1X TAE, 1.7% agarose gel
showing the amplification of Feline Calicivirus (FCV) at different
concentrations (Target). The size of the FCV target amplicon
corresponds to the 303 bp band represented by the provided
DNA Marker (M). NC = Negative Control
A ready-to-use system for the isolation and
detection of Feline Calicivirus Virus using end-point
RT-PCR
Norgen’s Feline Calicivirus Virus RT-PCR Detection Kit is a
ready-to-use system for the isolation and detection of FCV
from blood samples and nasal/throat swabs. First, the kit
contains components for the rapid isolation of total RNA,
including viral DNA, from the samples using spin-column
chromatography based on Norgen’s proprietary resin.
Second, the kit contains FCV Master Mix to allow forPCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time RT-PCR using melt
curves.
The FCV RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 303 bp
region of the viral genome. In addition, Norgen’s FCV RT-
PCR Detection Kit contains a second Mastermix, the RT-
PCR Control Master Mix, which can be used to identify
possible PCR inhibition and/or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The FCV RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood or
swabs
Contains two ready-to-use 2X RT-PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a FCV quantification standards
ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s FCV RT-PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s FCV RT-PCR Detection Kit
has been determined to cover concentrations from
100 ag to 1 ng
Under the conditions of the Norgen’s FCV RNA
Isolation procedure, Norgen’s FCV RT-PCR Detection
Kit covers a linear range from 100 copies to 1 x 106
copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
6
AnimalPathogenDetection
Feline Calicivirus RT-PCR Detection Kit Cat. # 43900
Description Cat # Size
End-Point PCR 43900 24 rxns
Real-Time PCR TM43900 48 rxns
Real-Time PCR SG43900 48 rxns
Primer Sets and Controls 43910 100 rxns
Feline Calicivirus Ordering information
NC
Target
M
FCV
M NC
Target
M
FCV
M
7. Figure 1. Sensitivity of Detection using the Feline Leukemia Virus
Detection Kit. A representative RT-qPCR Baseline Graph showing
the amplification of Feline Leukemia Virus RNA at different
concentrations (10-fold dilution series from 108 copies down to 104
copies).
Figure 2. Isolation and Detection of RNA from Cell-Containing
Media with No PCR Inhibition. Total RNA was isolated from HeLa
cell-containing media samples using Norgen's Total RNA
Purification Kit, which uses the same technology to isolate viral
RNA as Norgen's Feline Leukemia Virus RT-PCR Detection Kit. The
isolated HeLa RNA was then subjected to RT-qPCR using human
5S gene primers. To test the absence of PCR inhibitors usually
accompanying RNA isolated, an increasing amount from each
elution (1, 3 and 5 µL) was used as a template in the PCR
reaction. The red lines in the PCR baseline graph above
correspond to 1 µL of RNA used in a 20 µL reaction. The green
lines in the PCR baseline graph above correspond to 3 µL of RNA
used. The blue lines in the PCR baseline graph above correspond
to 5 µL of RNA used. The RNA could be detected in all cases,
indicating the high quality of the purified RNA.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
7
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of Feline Leukemia Virus using end-point
RT-PCR
Norgen’s Feline Leukemia Virus RT-PCR Detection Kit is a
ready-to-use system for the isolation and detection of
FeLV from blood samples and nasal/throat swabs. First,
the kit contains components for the rapid isolation of total
RNA, including viral RNA, from the samples using spin-
column chromatography based on Norgen’s proprietary
resin. Second, the kit contains FeLV Master Mix to allow for
PCR amplification, as well as a Control Master Mix to allow
for amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time RT-PCR using melt
curves.
The FeLV RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 310 bp
region of the viral genome. In addition, Norgen’s FeLV RT-
PCR Detection Kit contains a second Mastermix, the RT-
PCR Control Master Mix, which can be used to identify
possible PCR inhibition and/or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The FeLV RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood or
swabs
Contains two ready-to-use 2X RT-PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a FeLV quantification standards
ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s FeLV RT-PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s FeLV RT-PCR Detection
Kit has been determined to cover concentrations
from 100 ag to 1 ng
Under the conditions of the Norgen’s FeLV RNA
Isolation procedure, Norgen’s FeLV RT-PCR Detection
Kit covers a linear range from 100 copies to 1 x 106
copies.
Feline Leukemia Virus RT-PCR Detection Kit Cat. # 44000
Description Cat # Size
End-Point PCR 44000 24 rxns
Real-Time PCR TM44000 48 rxns
Real-Time PCR SG44000 48 rxns
Primer Sets and Controls 44010 100 rxns
Feline Leukemia Virus Ordering information
Cycle
0 5 10 15 20 25 30
Norm.Fluoro.
0.60
0.55
0.50
0.45
0.40
0.35
0.30
0.25
0.20
0.15
0.10
0.05
0.00
-0.05
Threshold
108
104
8. Figure 1. Sensitivity of Detection using the Feline
Immunodeficiency Virus RT-PCR Detection Kit. A representative
1X TAE, 1.7% agarose gel showing the amplification of Feline
Immunodeficiency Virus (FIV) at different concentrations (Target).
The size of the FIV target amplicon corresponds to the 318 bp
band represented by the provided DNA Marker (M). NC =
Negative Control
A ready-to-use system for the isolation and
detection of FVI using end-point RT-PCR
Norgen’s Feline Immunodeficiency Virus RT-PCR Detection
Kit is a ready-to-use system for the isolation and detection
of FIV from blood samples. First, the kit contains
components for the rapid isolation of total RNA, including
viral RNA, from the samples using spin-column
chromatography based on Norgen’s proprietary resin.
Second, the kit contains FIV Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time RT-PCR using melt
curves.
The FIV RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 318 bp
region of the viral genome. In addition, Norgen’s FIV RT-
PCR Detection Kit contains a second Mastermix, the RT-
PCR Control Master Mix, which can be used to identify
possible PCR inhibition and or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The FIV RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood
Contains two ready-to-use 2X RT-PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s FIV RT-PCR Detection Kit
was determined by analyzing a dilution series of a FIV
quantification standards ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s FIV RT-PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s FIV RT-PCR Detection Kit
has been determined to cover concentrations from
100 ag to 1 ng
Under the conditions of the Norgen’s FIV RNA Isolation
procedure, Norgen’s FIV RT-PCR Detection Kit covers
a linear range from 100 copies to 1 x 106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
8
AnimalPathogenDetection
Feline Immunodeficiency Virus RT-PCR Detection Kit Cat. # 44100
Description Cat # Size
End-Point PCR 44100 24 rxns
Real-Time PCR TM44100 48 rxns
Real-Time PCR SG44100 48 rxns
Primer Sets and Controls 44110 100 rxns
Feline Immunodeficiency Virus Ordering information
NC
Target
M
FIV
NC
Target
M
FIV
9. Figure 1. Sensitivity of Detection using the West Nile Virus RT-PCR
Detection Kit. A representative 1X TAE, 1.7% agarose gel showing
the amplification of West Nile Virus (WNV) at different
concentrations (Target). The size of the WNV target amplicon
corresponds to the 356 bp band represented by the provided
DNA Marker (M). NC = Negative Control
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
9
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of West Nile Virus using end-point RT-PCR
Norgen’s West Nile Virus RT-PCR Detection Kit is a ready-to
-use system for the isolation and detection of WNV from
blood or plasma samples. First, the kit contains
components for the rapid isolation of total RNA, including
viral RNA, from the samples using spin-column
chromatography based on Norgen’s proprietary resin.
Second, the kit contains WNV Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time RT-PCR using melt
curves.
The WNV RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 356 bp
region of the viral genome. In addition, Norgen’s WNV RT-
PCR Detection Kit contains a second Mastermix, the RT-
PCR Control Master Mix, which can be used to identify
possible PCR inhibition and/or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The WNV RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood or
plasma
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s WNV RT-PCR Detection
Kit was determined by analyzing a dilution series of a
WNV quantification standards ranging from 100 ag to
1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s WNV RT-PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s WNV RT-PCR Detection
Kit has been determined to cover concentrations
from 100 ag to 1 ng
Under the conditions of the Norgen’s WNV RNA
Isolation procedure, Norgen’s WNV RT-PCR Detection
Kit covers a linear range from 100 copies to 1 x 106
copies.
West Nile Virus RT-PCR Detection Kit Cat. # 44200
Description Cat # Size
End-Point PCR 44200 24 rxns
End-Point PCR Dx Dx44220 48 rxns
Real-Time PCR TM44200 48 rxns
Real-Time PCR SG44200 48 rxns
Primer Sets and Controls 44210 100 rxns
West Nile Virus Ordering information
NC
Target
M
WNV
NC
Target
M
WNV
10. Figure 1. Sensitivity of Detection using the Feline Herpes Virus PCR
Detection Kit. A representative 1X TAE, 1.7% agarose gel showing
the amplification of Feline Herpes Virus (FHV) at different
concentrations (Target). The size of the FHV target amplicon
corresponds to the 318 bp band represented by the provided
DNA Marker (M). NC = Negative Control
A ready-to-use system for the isolation and
detection of Feline Herpes Virus using end-point PCR
Norgen’s Feline Herpes Virus PCR Detection Kit is a ready-
to-use system for the isolation and detection of FHV from
plasma/serum samples, swabs and viral culture. First, the
kit contains components for the rapid isolation of total
DNA, including viral DNA, from the samples using spin-
column chromatography based on Norgen’s proprietary
resin. Second, the kit contains FHV Master Mix to allow for
PCR amplification, as well as a Control Master Mix to allow
for amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The FHV PCR Detection Mastermix contains reagents and
enzymes for the specific amplification of a 338 bp region
of the viral genome. In addition, Norgen’s FHV PCR
Detection Kit contains a second Mastermix, the PCR
Control Master Mix, which can be used to identify possible
PCR inhibition and/or inadequate isolation via a separate
PCR reaction with the use of the provided PCR control
(PCRC) or Isolation Control (IsoC), respectively. This kit is
designed to allow for the testing of 24 samples. The FHV
PCR Primer Set and Controls are also available separately
for end-point PCR detection.
Features and Benefits
Rapid isolation of high quality DNA from plasma/
serum, swabs, or viral culture
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s FHV PCR Detection Kit
was determined by analyzing a dilution series of a
FHV quantification standards ranging from 100 ag to 1
pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s FHV PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s FHV PCR Detection Kit
has been determined to cover concentrations from
100 ag to 1 ng
Under the conditions of the Norgen’s FHV DNA
Isolation procedure, Norgen’s FHV PCR Detection Kit
covers a linear range from 100 copies to 1 x
106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
10
AnimalPathogenDetection
Feline Herpes Virus PCR Detection Kit Cat. # 44300
Description Cat # Size
End-Point PCR 44300 24 rxns
Real-Time PCR TM44300 48 rxns
Real-Time PCR SG44300 48 rxns
Primer Sets and Controls 44310 100 rxns
Feline Herpes Virus Ordering information
NC
Target
M
FHV
NC
Target
M
FHV
11. Figure 1. Sensitivity of Detection using the FIPV RT-PCR Detection
Kit. A representative 1X TAE 1.5% agarose gel showing the
amplification of FIPV at different concentrations. The size of the
FIPV target amplicon corresponds to 304 bp as represented by
the provided DNA Marker (M). NTC = Negative Control.
Figure 2. Specificity of the primers used to detect FIPV. A 1.7 %
agarose gel is shown which indicates the specificity of the Feline
Infectious Peritonitis Virus RT-PCR Detection Kit. The specificity of
Norgen's Feline Infectious Peritonitis Virus RT-PCR Detection Kit is
first and foremost ensured by the selection of the Feline Infectious
Peritonitis Virus (FIPV)-specific primers, as well as the selection of
stringent reaction conditions. The primers were checked for
possible homologies in GenBank published sequences by
sequence comparison analyses. Lane 1 represents RNA transcript
fragment of the Feline calici virus. Lane 2 represents RNA
transcript fragment of the Feline Leukemia Virus. Lane 3
corresponds to RNA transcript fragment of Feline
Immunodeficiency Virus. Lane 4 corresponds to RNA transcript
fragment of Feline infectious peritonitis. Lane NTC corresponds to
negative (no template) control. No cross-reactivity of the target
primers was found for any of the tested RNA. The size of the FIP
target amplicon corresponds to the 304 bp band represented by
the provided DNA Marker (M).
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
11
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of FIPV using end-point RT-PCR
Norgen’s Feline Infectious Peritonitis Virus RT-PCR
Detection Kit is a ready-to-use system for the isolation and
detection of FIPV from blood samples. First, the kit
contains components for the rapid isolation of total RNA,
including viral RNA, from the samples using spin-column
chromatography based on Norgen’s proprietary resin.
Second, the kit contains FIPV Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time RT-PCR using melt
curves.
The FIPV RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 304 bp
region of the viral genome. In addition, Norgen’s FIPV RT-
PCR Detection Kit contains a second Mastermix, the RT-
PCR Control Master Mix, which can be used to identify
possible PCR inhibition and/or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The FIPV RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood
Contains two ready-to-use 2X RT-PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s FIPV RT-PCR Detection Kit
was determined by analyzing a dilution series of a FIV
quantification standards ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s FIV RT-PCR Detection Kit on a 1X TAE
1.7% agarose gel.
The linear range of Norgen’s FIVPV RT-PCR Detection
Kit has been determined to cover concentrations
from 100 ag to 1 ng
Under the conditions of the Norgen’s FIPV RNA
Isolation procedure, Norgen’s FIPV RT-PCR Detection
Kit covers a linear range from 100 copies to 1 x 106
copies.
Feline Infectious Peritonitis Virus RT-PCR Detection Kit Cat. # 44400
Description Cat # Size
End-Point PCR 44400 24 rxns
Real-Time PCR TM44400 48 rxns
Real-Time PCR SG44400 48 rxns
Primer Sets and Controls 44410 100 rxns
Feline Infectious Peritonitis Virus Ordering information
FIPV
(304 bp)
M 1 2 3 4 5 6 7 8 NTC M
FIPV
(304 bp)
M 1 2 3 4 5 6 7 8 NTC M
FIPV
(304 bp)
12. Figure 1. Sensitivity of Detection using the Dirofilaria immitis PCR
Detection Kit. A representative 1X TAE 1.5% agarose gel showing
the amplification of Dirofilaria immitis at different concentrations.
The size of the Dirofilaria immitis target amplicon corresponds to
276 bp as represented by the provided DNA Marker (M). NTC =
Negative Control.
Figure 2. Specificity of the Primers used to Detect Dirofilaria
immitis. A 1.7 % agarose gel is shown which indicates the
specificity of the Dirofilaria immitis PCR Detection Kit. The
specificity of Norgen's Dirofilaria immitis PCR Detection Kit is first
and foremost ensured by the selection of the Dirofilaria immitis
(DIR)-specific primers, as well as the selection of stringent
reaction conditions. The primers were checked for possible
homologies in GenBank published sequences by sequence
comparison analyses. Lane 1 corresponds to cloned PCR
fragment of the Dirofilaria immitis. Lane 2 corresponds to cloned
PCR fragment of Toxoplasma gondii. Lane 3 corresponds to
genomic DNA of Leptospria interrogans. Lane 4 corresponds to
cloned PCR fragment of the Bordetella. Lane NTC corresponds to
negative (no template) control. No cross-reactivity of the target
primers was found for any of the tested DNA. The size of
the Dirofilaria target amplicon corresponds to the 276 bp band
represented by the provided DNA Marker (M).
A ready-to-use system for the isolation and
detection of Dirofilaria immitis using end-point PCR
Norgen’s Dirofilaria immitis PCR Detection Kit is a ready-to-
use system for the isolation and detection of Dirofilaria
immitis from blood samples. First, the kit contains
components for the rapid isolation of total DNA from the
samples using spin-column chromatography. Second, the
kit contains Dirofilaria immitis Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The Dirofilaria immitis PCR Detection Mastermix contains
reagents and enzymes for the specific amplification of a
276 bp region of the parasite genome. In addition,
Norgen’s Dirofilaria immitis PCR Detection Kit contains a
second Mastermix, the PCR Control Master Mix, which
can be used to identify possible PCR inhibition and/or
inadequate isolation via a separate PCR reaction with the
use of the provided PCR control (PCRC) or Isolation
Control (IsoC), respectively. This kit is designed to allow for
the testing of 24 samples. The Dirofilaria immitisPCR Primer
Set and Controls are also available separately for end-
point PCR detection.
Features and Benefits
Rapid isolation of high quality RNA from blood
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s Dirofilaria immitis PCR
Detection Kit was determined by analyzing a dilution
series of a Dirofilaria immitis quantification standards
ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Dirofilaria immitis PCR Detection Kit on
a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Dirofilaria immitis PCR
Detection Kit has been determined to cover
concentrations from 100 ag to 1 ng
Under the conditions of the Norgen’s Dirofilaria
immitis DNA Isolation procedure, Norgen’s Dirofilaria
immitis PCR Detection Kit covers a linear range from
100 copies to 1 x 106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
12
AnimalPathogenDetection
Dirofilaria immitis PCR Detection Kit Cat. # 44500
Description Cat # Size
End-Point PCR 44500 24 rxns
Real-Time PCR TM44500 48 rxns
Real-Time PCR SG44500 48 rxns
Primer Sets and Controls 44510 100 rxns
Dirofilaria immitis Ordering information
Dirofilaria
(276 bp)
M 1 2 3 4 5 6 7 8 NTC M
Dirofilaria
(276 bp)
M 1 2 3 4 5 6 7 8 NTC M
Dirofilaria
(276 bp)
13. Figure 1. Sensitivity of Detection using the Leptospira
interrogans PCR Detection Kit. A representative 1X TAE 1.5%
agarose gel showing the amplification of Leptospira
interrogans at different concentrations. The size of
the Leptospira target amplicon corresponds to 350 bp as
represented by the provided DNA Marker (M). NTC = Negative
Control.
Figure 2. Specificity of the Primers used to Detect Leptospira
interrogans. A 1.7 % agarose gel is shown which indicates the
specificity of the Leptospira interrogans PCR Detection Kit. The
specificity of Norgen's Leptospira interrogans PCR Detection Kit is
first and foremost ensured by the selection of the
Leptospira interrogans (Tox)-specific primers, as well as the
selection of stringent reaction conditions. The primers were
checked for possible homologies in GenBank published
sequences by sequence comparison analyses. Lane 1
corresponds to cloned PCR fragment of the Dirofilaria immitis.
Lane 2 corresponds to cloned PCR fragment of the Toxoplasma
gondii. Lane 3 corresponds to genomic DNA of Leptospria
interrogans. Lane 4 corresponds to cloned PCR fragment of the
Bordetella. Lane 5 corresponds to cloned PCR fragment of
the Borrelia garinil. Lane NTC corresponds to negative (no
template) control. No cross-reactivity of the target primers was
found for any of the tested DNA. The size of the Leptospira target
amplicon corresponds to the 350 bp band represented by the
provided DNA Marker (M).
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
13
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of Leptospira interrogans using end-point
PCR
Norgen’s Leptospira interrogans PCR Detection Kit is a
ready-to-use system for the isolation and detection
of Leptospira interrogans from urine samples. First, the kit
contains components for the rapid isolation of total DNA
from the samples using spin-column chromatography
based on Norgen’s proprietary resin. Second, the kit
contains Leptospira interrogans Master Mix to allow for
PCR amplification,as well as a Control Master Mix to allow
for amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The Leptospira interrogans PCR Detection Mastermix
contains reagents and enzymes for the specific
amplification of a 350 bp region of the bacterial genome.
In addition, Norgen’s Leptospira interrogans PCR
Detection Kit contains a second Mastermix, the PCR
Control Master Mix, which can be used to identify possible
PCR inhibition and/or inadequate isolation via a separate
PCR reaction with the use of the provided PCR control
(PCRC) or Isolation Control (IsoC), respectively. This kit is
designed to allow for the testing of 24 samples.
The Leptospira interrogans PCR Primer Set and Controls
are also available separately for end-point PCR
detection.
Features and Benefits
Rapid isolation of high quality DNA from urine
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a Leptospira quantification standards
ranging from 1pg to 10 ng.
Each dilution has been tested in replicates (n = 4) on
a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Leptospira
interrogans PCR Detection Kit has been determined
to cover concentrations from 1pg to 10 ng.
Under the conditions of the Leptospira DNA Isolation
procedure, Norgen’s Leptospira interrogans PCR
Detection Kit covers a linear range from 100 copies to
1 x 106 copies.
Leptospira interrogans PCR Detection Kit Cat. # 44600
Description Cat # Size
End-Point PCR 44600 24 rxns
Real-Time PCR TM44600 48 rxns
Real-Time PCR SG44600 48 rxns
Primer Sets and Controls 44610 100 rxns
Leptospira interrogans Ordering information
Leptospira
(350bp)
M 1 2 3 4 5 6 7 8 NTC M
Leptospira
(350bp)
M 1 2 3 4 5 6 7 8 NTC M
Leptospira
(350 bp)
14. Figure 1. Sensitivity of Detection using the Toxoplasma gondii PCR
Detection Kit. A representative 1X TAE 1.5% agarose gel showing
the amplification of Toxoplasma at different concentrations. The
size of the Toxoplasma target amplicon corresponds to 303 bp as
represented by the provided DNA Marker (M). NTC = Negative
Control.
Figure 2. Specificity of the Primers used to Detect Toxoplasma
gondii . A 1.7 % agarose gel is shown which indicates the
specificity of the Toxoplasma gondii PCR Detection Kit. The
specificity of Norgen's Toxoplasma gondii PCR Detection Kit is first
and foremost ensured by the selection of the Toxoplasma
gondii (Tox)-specific primers, as well as the selection of stringent
reaction conditions. The primers were checked for possible
homologies in GenBank published sequences by sequence
comparison analyses. Lane 1 corresponds to cloned PCR
fragment of the Dirofilaria immitis. Lane 2 corresponds to cloned
PCR fragment of the Toxoplasma gondii. Lane 3 corresponds to
genomiic DNA of Leptospria interrogans. Lane 4 corresponds to
cloned PCR fragment of the Bordetella. Lane NTC corresponds to
negative (no template) control. No cross-reactivity of the target
primers was found for any of the tested DNA. The size of
the Toxoplasma target amplicon corresponds to the 303 bp
band represented by the provided DNA Marker (M).
A ready-to-use system for the isolation and
detection of Toxoplasma gondii using end-point
PCR
Norgen’s Toxoplasma gondii PCR Detection Kit is a ready-
to-use system for the isolation and detection
of Toxoplasma gondii from blood samples. First, the kit
contains components for the rapid isolation of total DNA
from the samples using spin-column chromatography.
Second, the kit contains Toxoplasma gondii Master Mix to
allow for PCR amplification, as well as a Control Master
Mix to allow for amplification of both an isolation control
and a PCR control. The amplified PCR products are then
detected using agarose gel electrophoresis. Alternatively,
detection can be performed based on real-time PCR
using melt curves.
The Toxoplasma gondii PCR Detection Mastermix contains
reagents and enzymes for the specific amplification of a
303 bp region of the parasite genome. In addition,
Norgen’s Toxoplasma gondii PCR Detection Kit contains a
second Mastermix, the PCR Control Master Mix, which
can be used to identify possible PCR inhibition and/or
inadequate isolation via a separate PCR reaction with the
use of the provided PCR control (PCRC) or Isolation
Control (IsoC), respectively. This kit is designed to allow for
the testing of 24 samples. The Toxoplasma gondiiPCR
Primer Set and Controls are also available separately for
end-point PCR detection.
Features and Benefits
Rapid isolation of high quality DNA from blood
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s Toxoplasma gondii PCR
Detection Kit was determined by analyzing a dilution
series of a Toxoplasma gondii quantification
standards ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Toxoplasma gondii PCR Detection Kit
on a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Toxoplasma gondii PCR
Detection Kit has been determined to cover
concentrations from 100 ag to 1 ng.
Under the conditions of the Norgen’s Toxoplasma
gondii DNA Isolation procedure, Norgen’s Dirofilaria
immitis PCR Detection Kit covers a linear range from
100 copies to 1 x 106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
14
AnimalPathogenDetection
Toxoplasma gondii PCR Detection Kit Cat. # 44700
Description Cat # Size
End-Point PCR 44700 24 rxns
Real-Time PCR TM44700 48 rxns
Real-Time PCR SG44700 48 rxns
Primer Sets and Controls 44710 100 rxns
Toxoplasma gondii Ordering information
Toxoplasma
(303 bp)
M 1 2 3 4 5 6 7 8 NTC M
Toxoplasma
(303 bp)
M 1 2 3 4 5 6 7 8 NTC M
Taxoplasma
(303 bp)
15. Figure 1. Sensitivity of Detection using the Bordetella
bronchiseptica PCR Detection Kit. A representative 1X TAE, 1.5%
agarose gel showing the amplification ofBordetella
bronchiseptica at different concentrations. The size of
the Bordetella bronchiseptica target amplicon corresponds to
the 277 bp band represented by the provided DNA Marker (M).
NC = Negative Control.
Figure 2. Specificity of the Primers used to Detect Bordetella
bronchiseptica . A 1.5% agarose gel is shown which indicates the
specificity of the Bordetella bronchiseptica PCR Detection Kit.
The specificity of Norgen's Bordetella bronchiseptica PCR
Detection Kit is first and foremost ensured by the selection of
theBordetella bronchiseptica-specific primers, as well as the
selection of stringent reaction conditions. The primers were
checked for possible homologies in GenBank published
sequences by sequence comparison analyses. Lane A represents
RNA transcript fragment of Rabies. Lane B corresponds to cloned
PCR fragment of FPV Virus. Lanes C corresponds to cloned PCR
fragment of Chlamydophila felis. Lane D corresponds
to Bordetella bronchiseptica positive control. Lanes E
corresponds to cloned PCR fragment of Feline panleukopenia
virus. Lanes F corresponds to cloned PCR fragment of B.
burgdorferi. Lane G corresponds to negative (no template)
control. No cross-reactivity of the target primers was found for
any of the tested RNA/DNA. The size of the FeLV target amplicon
corresponds to the 310 bp band represented by the provided
DNA Marker (M).
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
15
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of Bordetella bronchiseptica using end-
point PCR
Norgen’s Bordetella bronchiseptica PCR Detection Kit is a
ready-to-use system for the isolation and detection
of Bordetella bronchiseptica from nasal exudates swabs
and viral culture. First, the kit contains components for the
rapid isolation of total DNA, including bacteril DNA, from
the samples using spin-column chromatography based
on Norgen’s proprietary resin. Second, the kit contains
Bordetella bronchiseptica Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The Bordetella bronchiseptica PCR Detection Mastermix
contains reagents and enzymes for the specific
amplification of a 277 bp region of the viral genome. In
addition, Norgen’s Bordetella bronchiseptica PCR
Detection Kit contains a second Mastermix, the PCR
Control Master Mix, which can be used to identify possible
PCR inhibition and/or inadequate isolation via a separate
PCR reaction with the use of the provided PCR control
(PCRC) or Isolation Control (IsoC), respectively. This kit is
designed to allow for the testing of 24 samples.
The Bordetella bronchiseptica PCR Primer Set and
Controls are also available separately for end-point PCR
detection.
Features and Benefits
Rapid isolation of high quality DNA from nasal
exudates or swabs
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a Bordetella bronchiseptica
quantification standards ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4) on
a 1X TAE 1.7% agarose gel.
The linear range has been determined to cover
concentrations from 100 ag to 1 ng.
Under the conditions of the Norgen’s DNA Isolation
procedure, this kit covers a linear range from 100
copies to 1 x 106 copies.
Bordetella bronchiseptica PCR Detection Kit Cat. # 44900
Description Cat # Size
End-Point PCR 44900 24 rxns
Real-Time PCR TM44900 48 rxns
Real-Time PCR SG44900 48 rxns
Primer Sets and Controls 44910 100 rxns
Bordetella bronchiseptica Ordering information
M Pos NC M
BORD
Target
M Pos NC M
BORD
Target
16. Figure 1. Sensitivity of Detection using the Canine Parvovirus PCR
Detection Kit. A representative 1X TAE 1.7% agarose gel showing
the amplification of Canine parvo at different concentrations.
The size of the Canine parvovirus target amplicon corresponds to
283 bp as represented by the provided DNA Marker (M). NTC =
Negative Control.
Figure 2. Specificity of the Primers used to Detect Canine
Parvovirus. A 1.5% agarose gel is shown which indicates the
specificity of the Canine Parvovirus PCR Detection Kit. The
specificity of Norgens Canine Parvovirus PCR Detection Kit is first
and foremost ensured by the selection of the parvovirus-specific
primers, as well as the selection of stringent reaction conditions.
The primers were checked for possible homologies in GenBank
published sequences by sequence comparison analyses. Lane A
represents RNA transcript fragment of the Rabies. Lane B
corresponds to cloned PCR fragment of FPV Virus. Lanes C
corresponds to cloned PCR fragment of Chlamydophila felis.
Lane D corresponds to a cloned fragment of Bordetella
bronchiseptica. Lanes E corresponds to cloned PCR fragment of
Canine Parvovirus. Lanes F corresponds to cloned PCR fragment
of B. burgdorferi. Lane G corresponds to negative (no template)
control. No cross-reactivity of the target primers was found for
any of the tested RNA/DNA. The size of the Canine Parvovirus
target amplicon corresponds to the 283 bp band represented by
the provided DNA Marker (M).
A ready-to-use system for the isolation and
detection of Canine Parvovirus using end-point PCR
Norgen’s Canine Parvovrus PCR Detection Kit is a ready-
to-use system for the isolation and detection of Canine
Parvovirus from blood samples. First, the kit contains
components for the rapid isolation of total DNA from the
samples using spin-column chromatography based on
Norgen’s proprietary resin. Second, the kit contains
Parvovirus Master Mix to allow for PCR amplification, as
well as a Control Master Mix to allow for amplification of
both an isolation control and a PCR control. The amplified
PCR products are then detected using agarose gel
electrophoresis. Alternatively, detection can be
performed based on real-time PCR using melt curves.
The Canine Parvovirus PCR Detection Mastermix contains
reagents and enzymes for the specific amplification of a
283 bp region of the viral genome. In addition, Norgen’s
Canine Parvovirus PCR Detection Kit contains a second
Mastermix, the PCR Control Master Mix, which can be
used to identify possible PCR inhibition and/or inadequate
isolation via a separate PCR reaction with the use of the
provided PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The Canine Parvovirus PCR Primer Set and
Controls are also available separately for end-point PCR
detection.
Features and Benefits
Rapid isolation of high quality DNA from blood
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s Canine Parvovirus PCR
Detection Kit was determined by analyzing a dilution
series of a Canine Parvovirus quantification standards
ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Canine Parvovirus PCR Detection Kit
on a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Canine Parvovirus PCR
Detection Kit has been determined to cover
concentrations from 100 ag to 1 ng
Under the conditions of the Norgen’s Canine
Parvovirus DNA Isolation procedure, Norgen’s Canine
Parvovirus PCR Detection Kit covers a linear range
from 100 copies to 1 x 106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
16
AnimalPathogenDetection
Canine Parvovirus PCR Detection Kit Cat. # 45000
Description Cat # Size
End-Point PCR 45000 24 rxns
Real-Time PCR TM44700 48 rxns
Real-Time PCR SG44700 48 rxns
Primer Sets and Controls 45010 100 rxns
Canine Parvovirus Ordering information
M Pos NC M
Canine Parvovirus
Target
M Pos NC M
Canine Parvovirus
Target
17. Figure 1. Sensitivity of Detection using the Feline Panleukopenia
Virus PCR Detection Kit. A representative 1.5X TAE 1.7% agarose
gel showing the amplification of FPV under different
concentrations using the 2X FPV Detection PCR Mastermix. The
size of the FPV target amplicon corresponds to 301 bp as
represented by the provided DNA Marker (M). NC = Negative
Control.
Figure 2. Specificity of the Primers used to Detect FPV. A 1.5%
agarose gel is shown which indicates the specificity of the FPV
PCR Detection Kit. The specificity of Norgen's FPV PCR Detection
Kit is first and foremost ensured by the selection of the FPV-
specific primers, as well as the selection of stringent reaction
conditions. The primers were checked for possible homologies in
GenBank published sequences by sequence comparison
analyses. Lane A represents RNA transcript fragment of the
Rabies. Lane B corresponds to cloned PCR fragment of FPV Virus.
Lanes C corresponds to cloned PCR fragment ofChlamydophila
felis. Lane D corresponds to Bordetella bronchiseptica positive
control. Lanes E corresponds to cloned PCR fragment of Canine
Parvovirus. Lanes F corresponds to cloned PCR fragment of B.
burgdorferi. Lane G corresponds to negative (no template)
control. No cross-reactivity of the target primers was found for
any of the tested RNA/DNA. The size of the FPV target amplicon
corresponds to the 301 bp band represented by the provided
DNA Marker (M).
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
17
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of Feline Panleukopenia Virus using end-
point PCR
Norgen’s Feline Panleukopenia Virus PCR Detection Kit is a
ready-to-use system for the isolation and detection of
FPV from blood samples. First, the kit contains
components for the rapid isolation of total DNA from the
samples using spin-column chromatography. Second, the
kit contains FPV Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The Feline Panleukopenia Virus PCR Detection Mastermix
contains reagents and enzymes for the specific
amplification of a 301 bp region of the viral genome. In
addition, Norgen’s Feline Panleukopenia Virus PCR
Detection Kit contains a second Mastermix, the PCR
Control Master Mix, which can be used to identify possible
PCR inhibition and/or inadequate isolation via a separate
PCR reaction with the use of the provided PCR control
(PCRC) or Isolation Control (IsoC), respectively. This kit is
designed to allow for the testing of 24 samples. The Feline
Panleukopenia Virus PCR Primer Set and Controls are also
available separately for end-point PCR detection.
Features and Benefits
Rapid isolation of high quality DNA from blood
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a Feline Panleukopenia Virus
quantification standards ranging from 100 ag to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Feline Panleukopenia Virus PCR
Detection Kit on a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Feline Panleukopenia
Virus PCR Detection Kit has been determined to
cover concentrations from 100 ag to 1 ng
Under the conditions of the Norgen’s Feline
Panleukopenia Virus DNA Isolation procedure,
Norgen’s Feline Panleukopenia Virus PCR Detection
Kit covers a linear range from 100 copies to 1 x
106 copies.
Feline Panleukopenia Virus PCR Detection Kit Cat. # 45100
Description Cat # Size
End-Point PCR 45100 24 rxns
Real-Time PCR TM45100 48 rxns
Real-Time PCR SG45100 48 rxns
Primer Sets and Controls 45110 100 rxns
Feline Panleukopenia Virus Ordering information
M Pos NC M
FPV
Target
M Pos NC M
FPV
Target
18. Figure 1. Sensitivity of Detection using the Borrelia burgdorferi PCR
Detection Kit. A representative 1.5X TAE 1.7% agarose gel
showing the amplification of B. burgdorferi under different
concentration using the 2XB. burgdorferi Detection PCR
Mastermix. The size of the B. burgdorferi target amplicon
corresponds to 277 bp as represented by the provided DNA
Marker (M). NC = Negative Control.
Figure 2. Specificity of the Primers used to Detect B. burgdorferi. A
1.5% agarose gel is shown which indicates the specificity of the B.
burgdorferi PCR Detection Kit. The specificity of Norgens B.
burgdorferiPCR Detection Kit is first and foremost ensured by the
selection of the B. burgdorferi-specific primers, as well as the
selection of stringent reaction conditions. The primers were
checked for possible homologies in GenBank published
sequences by sequence comparison analyses. Lane A represents
RNA transcript fragment of the Rabies. Lane B corresponds to
cloned PCR fragment of FPV Virus. Lanes C corresponds to
cloned PCR fragment ofChlamydophila felis. Lane D corresponds
to Bordetella bronchiseptica positive control. Lanes E
corresponds to cloned PCR fragment of Canine Parvovirus. Lanes
F corresponds to cloned PCR fragment of B. burgdorferi. Lane G
corresponds to negative (no template) control. No cross-
reactivity of the target primers was found for any of the tested
RNA/DNA. The size of the B. burgdorferi target amplicon
corresponds to the 277 bp band represented by the provided
DNA Marker (M).
A ready-to-use system for the isolation and
detection of Borrelia burgdorferi using end-point
PCR
Norgen’s Borrelia burgdorferi PCR Detection Kit is a ready-
to-use system for the isolation and detection of Borrelia
burgdorferi from urine samples. First, the kit contains
components for the rapid isolation of total DNA from the
samples using spin-column chromatography based on
Norgen’s proprietary resin. Second, the kit
contains Borrelia burgdorferi Master Mix to allow for PCR
amplification, as well as a Control Master Mix to allow for
amplification of both an isolation control and a PCR
control. The amplified PCR products are then detected
using agarose gel electrophoresis. Alternatively, detection
can be performed based on real-time PCR using melt
curves.
The Borrelia burgdorferi PCR Detection Mastermix contains
reagents and enzymes for the specific amplification of a
277 bp region of the bacterial genome. In addition,
Norgen’s Borrelia burgdorferi PCR Detection Kit contains a
second Mastermix, the PCR Control Master Mix, which
can be used to identify possible PCR inhibition and/or
inadequate isolation via a separate PCR reaction with the
use of the provided PCR control (PCRC) or Isolation
Control (IsoC), respectively. This kit is designed to allow for
the testing of 24 samples. The Borrelia burgdorferi PCR
Primer Set and Controls are also available separately for
end-point PCR detection.
Features and Benefits
Rapid isolation of high quality DNA from urine
Contains two ready-to-use 2X PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range was determined by analyzing a
dilution series of a Borrelia burgdorferi quantification
standards ranging from 1pg to 10 ng.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Borrelia burgdorferi PCR Detection Kit
on a 1X TAE 1.7% agarose gel.
The linear range of Norgen’s Borrelia burgdorferi PCR
Detection Kit has been determined to cover
concentrations from 1pg to 10 ng.
Under the conditions of the Norgen’s Borrelia
burgdorferi DNA Isolation procedure,
Norgen’s Borrelia burgdorferi PCR Detection Kit covers
a linear range from 100 copies to 1 x 106 copies.
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
18
AnimalPathogenDetection
Borrelia burgdorferi PCR Detection Kit Cat. # 45200
Description Cat # Size
End-Point PCR 45200 24 rxns
Real-Time PCR TM44700 48 rxns
Real-Time PCR SG44700 48 rxns
Primer Sets and Controls 45210 100 rxns
Borrelia burgdorferi Ordering information
M NC Pos
B. burgdorferi
M NC Pos
B. burgdorferi
19. Figure 1. Sensitivity of Detection using the Rabies RT-PCR
Detection KitA representative 1.5X TAE 1.7% agarose gel showing
the amplification of Rabies under different concentration using
the 2X Rabies Detection RT-PCR Mastermix. The size of the Rabies
target amplicon corresponds to 280 bp as represented by the
provided DNA Marker (M). NC = Negative Control.
Figure 2. Specificity of the Primers used to Detect Rabies. A 1.5%
agarose gel is shown which indicates the specificity of the Rabies
RT- PCR Detection Kit. The specificity of Norgen's Rabies RT-PCR
Detection Kit is first and foremost ensured by the selection of the
Rabies-specific primers, as well as the selection of stringent
reaction conditions. The primers were checked for possible
homologies in GenBank published sequences by sequence
comparison analyses. Lane A corresponds to RNA transcript
fragment of the Rabies. Lane B corresponds to cloned PCR
fragment of FPV Virus. Lanes C corresponds to cloned PCR
fragment of Chlamydophila felis. Lane D corresponds
to Bordetella bronchiseptica positive control. Lanes E represents
cloned PCR fragment ofCanine Parvovirus. Lanes F corresponds
to cloned PCR fragment of B. burgdorferi. Lane G corresponds to
negative (no template) control. No cross-reactivity of the target
primers was found for any of the tested RNA/DNA. The size of the
Rabies target amplicon corresponds to the 280 bp band
represented by the provided DNA Marker (M).
For research use only and NOT intended for in vitro diagnostics
Toll Free in North America: 1-866-667-4362 www.norgenbiotek.com
19
AnimalPathogenDetection
A ready-to-use system for the isolation and
detection of Rabies using end-point RT-PCR
Norgen’s Rabies RT-PCR Detection Kit is a ready-to-use
system for the isolation and detection of rabies from
blood samples. First, the kit contains components for the
rapid isolation of total RNA, including viral RNA, from the
samples using spin-column chromatography based on
Norgen’s proprietary resin. Second, the kit contains Rabies
Master Mix to allow for PCR amplification, as well as a
Control Master Mix to allow for amplification of both an
isolation control and a PCR control. The amplified PCR
products are then detected using agarose gel
electrophoresis. Alternatively, detection can be
performed based on real-time RT-PCR using melt curves.
The Rabies RT-PCR Detection Mastermix contains reagents
and enzymes for the specific amplification of a 280 bp
region of the viral genome. In addition, Norgen’s Rabies
RT-PCR Detection Kit contains a second Mastermix, the
Control RT-PCR Master Mix, which can be used to identify
possible PCR inhibition and/or inadequate isolation via a
separate RT-PCR reaction with the use of the provided
PCR control (PCRC) or Isolation Control (IsoC),
respectively. This kit is designed to allow for the testing of
24 samples. The Rabies RT-PCR Primer Set and Controls are
also available separately for end-point RT- PCR detection.
Features and Benefits
Rapid isolation of high quality DNA from blood
Contains two ready-to-use 2X RT-PCR Master Mixes
High sensitivity and specificity
Includes an isolation control and a PCR control
Primer set and controls also available separately
Ideal for use in:
1. Surveillance of Drug Resistant Pathogens
2. Epidemiologial Studies
3. Field Surveillance of Pathogens
4. Surveys
Linear Range
The linear range of Norgen’s Rabies RT-PCR Detection
Kit was determined by analyzing a dilution series of a
Rabies quantification standards ranging from 100 ag
to 1 pg.
Each dilution has been tested in replicates (n = 4)
using Norgen’s Rabies RT-PCR Detection Kit on a 1X
TAE 1.7% agarose gel.
The linear range of Norgen’s Rabies RT-PCR Detection
Kit has been determined to cover concentrations
from 100 ag to 1 ng
Under the conditions of the Norgen’s Rabies RNA
Isolation procedure, Norgen’s Rabies RT-PCR
Detection Kit covers a linear range from 100 copies to
1 x 106 copies.
Rabies RT-PCR Detection Kit Cat. # 45300
Description Cat # Size
End-Point PCR 45300 24 rxns
Real-Time PCR TM45300 48 rxns
Real-Time PCR SG45300 48 rxns
Primer Sets and Controls 45310 100 rxns
Rabies Ordering information
M Pos NC M
Rabies
Target
M Pos NC M
Rabies
Target
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Phone: (905) 227-8848
Toll Free: 1-866-NORGENB (667-4362)
Fax: (905) 227-1061
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