RTGS allows for high-value, real-time fund transfers of a minimum Rs. 2 lakh amount individually, providing urgent same-day settlement. NEFT is a nationwide system for one-to-one fund transfers of amounts up to Rs. 10 lacs processed in batches throughout the day at slower speeds than RTGS. The key differences are that RTGS supports higher minimum amounts for urgent real-time settlement, while NEFT allows for lower value fund transfers processed in batches at slower speeds.