Position Summary:
Responsible for developing, implementing and managing systems to mitigate the risk of experiencing unacceptable levels of bioburden in products. This includes establishing best practices, process controls and environmental monitoring programs that reduce the potential for microbial product contamination. Also responsible for training manufacturing associates on aseptic technique and monitoring and coaching production associates at their work stations.
Key Responsibilities:
•Microbial sampling, identification & bioburden mapping of production facilities.
•Bioburden challenge studies for existing and new product formulations.
•Bioburden risk assessment and validation for new product development process.
•Aseptic process development and process control.
•Environmental and water monitoring of Rockford and Wisconsin sites.
•Interpret test results and initiate corrective actions as needed.
•Assist with equipment and process cleaning validations.
•Clean room management inclusive of mentoring and supervising 23 clean room associates.
•Microbial test-method development as per USP test method suitability.
•Contamination investigations & implementation of corrective actions.
•Prepare reports and present data with conclusions.
•Maintain microbiology laboratory equipment and supplies.
Also efficiently performed Preservative Efficacy Challenge Tests, Environmental monitoring, LAL Endotoxin testing (Kinetic & Gel Clot methods), Microbial Ingress Integrity Testing (both Dye Penetration & Bacterial Challenge testing), Microbial Identification using Biolog, Mycoplasma testing of products using Luminometer, Bioburden & Water testing.
Welcome to the M Lab™ Collaboration Centers – where customers can use non-GMP lab spaces to operate equipment, evaluate processes and receive real-time technical support without disrupting production.
Plan your visit: www.merckmillipore.com/mlab
Our goal is to provide an environment where patients are treated with the compassion and understanding they deserve. We want to make the process informative yet simple.
Position Summary:
Responsible for developing, implementing and managing systems to mitigate the risk of experiencing unacceptable levels of bioburden in products. This includes establishing best practices, process controls and environmental monitoring programs that reduce the potential for microbial product contamination. Also responsible for training manufacturing associates on aseptic technique and monitoring and coaching production associates at their work stations.
Key Responsibilities:
•Microbial sampling, identification & bioburden mapping of production facilities.
•Bioburden challenge studies for existing and new product formulations.
•Bioburden risk assessment and validation for new product development process.
•Aseptic process development and process control.
•Environmental and water monitoring of Rockford and Wisconsin sites.
•Interpret test results and initiate corrective actions as needed.
•Assist with equipment and process cleaning validations.
•Clean room management inclusive of mentoring and supervising 23 clean room associates.
•Microbial test-method development as per USP test method suitability.
•Contamination investigations & implementation of corrective actions.
•Prepare reports and present data with conclusions.
•Maintain microbiology laboratory equipment and supplies.
Also efficiently performed Preservative Efficacy Challenge Tests, Environmental monitoring, LAL Endotoxin testing (Kinetic & Gel Clot methods), Microbial Ingress Integrity Testing (both Dye Penetration & Bacterial Challenge testing), Microbial Identification using Biolog, Mycoplasma testing of products using Luminometer, Bioburden & Water testing.
Welcome to the M Lab™ Collaboration Centers – where customers can use non-GMP lab spaces to operate equipment, evaluate processes and receive real-time technical support without disrupting production.
Plan your visit: www.merckmillipore.com/mlab
Our goal is to provide an environment where patients are treated with the compassion and understanding they deserve. We want to make the process informative yet simple.
DNA damage repair Neil3 gene Knockout in MOLT-4iosrjce
RNAi is superannuated cellular mechanism that protect organism against viruses that replicate
through double- stranded RNA. RNAi can be used to diminish gene expression from plasmid expressing and
inserted sequence repeat. A stable harpin would be expressed after the vector was integrated into the genome.
In this paper a shiRNA expressing vector for Neil3 was designed and developed which is capable of replication
in MOLT-4. This shiRNA vector had the ability to arose the RNAi pathway, and reduce the gene expression of
Neil3. This was assessed by using pSilence 4.1CMV as a vector, and Gapdh as positive control.
UV Mutagenesis Enhanced Biotransformation Efficiency of Rutin to Isoquercitri...iosrjce
In order to obtain high biotransformation efficiency of rutin to isoquercitrin (quercetin-3-Oglucoside),
Bacillus litoralis C44 was treated by UV mutagenesis to screen the thermo- and alkali-tolerant
mutants, for these conditions allow for a very high substrate concentration. The optimal mutagen dose for strain
C44 was irradiation for 50s with a 15W UV lamp from 30 cm away. The mutants were preliminary screened by
quantitative TLC, and 16 mutant strains were faced to second-screening by HPLC. As a result, a genetic stable
mutant strain UV-2-45 was obtained, which got a biotransformation rate of 3.9 times more than the original
strain Bacillus litoralis C44, and its mole yield reached as high as 91% from 3 g/L of rutin in glycine-sodium
hydroxide buffer (pH 9.0) at 45°C for 2 days.
Microbiological Investigations of Selected Flies of Public Health Importance ...iosrjce
Bacteria associated with flies of public health importance in Nigeria are not well known and their
ecology is also not well understood. We aim to determine the bacteria associated with flies of waste dump site.
Three flies of public health significance were collected from a waste dump site of the Rivers State University of
Science and Technology, Port Harcourt. The three dipterous flies were Luciliasericata, Chrysomyasp and
Musca domestica..The three flies were all of medical importance.The microbial load on three species of flies
was investigated using standard plate count methods. The fly samples were collected from the Post Graduate
Entomology Laboratory was cultured to isolate and identify the microbes associated with them. The samples
were analyzed for total heterotrophic bacteria and fungi counts. The study revealed high heterotrophic bacteria
and fungi counts in all three species of the flies used, with Musca domestica having the highest count of 2.9 X
109Cfu/gram and Chrysomyasp with the least count of 3.4 x 10 5Cfu/g and fungi counts ranged from 3.1 X
103Cfu/g to 2.9 X 105Cfu/g. The bacteria isolated from these samples includes: Escherichia coli,
Pseudomonassp,, Bacillussp, Enterobactersp, Staphylococcussp,Salmonellasp, Proteussp, and Klebsiellasp,
while the fungi species isolated includes: Penicilliumsp,
Aspergillussp,Rhizopussp,Cladosporiumsp,Aspergillusflavus, Aspergillusniger, Fusariumsp and Trichoderma
sp.
I am in quest of senior level assignments as Functional Lead - Quality Control/ Quality Assurance with an organization of repute in Denmark or nearby Schengen countries.
1. D. REVATHI
E-Mail: reva1990@gmail.com / revathidhanashekar@yahoo.com
Mobile: +91 – 9573590331 / Tel.: 040 – 27208101
Seeking potential challenges that can help strengthen my fervour towards Biotechnology
AAANNN OOOVVVEEERRRVVVIIIEEEWWW
M.Tech in the field of Biochemical Engineering & Biotechnology and undertaking a project at IICT in the field of
Parameter Optimization for Bioprocess.
Qualified B.Tech.
In Plant trainee with nearly 3 months experience in Production and Quality control of milk and milk by products at
GMP complying production plant. And trained at a renowned Contract Research Organisation (CRO) affiliated with GLP
certification under Biotech Industrial Training Program 2012-2013 for 6 months.
Well versed in conducting experiments such as Bacterial Reverse Mutation (Ames test), Chromosomal Aberration in
Human Lymphocytes and Mice Lymphocytes, Micronuclei Assay in Mice/ Rat which are conducted for toxicological
screening of potential genotoxic compounds.
I have been able to attend workshops on Intellectual Property Rights, Patent Assessment and Regulatory Affairs
conducted by Patent Information Centre (PIC), A.P State Council Of Science & Technology (APCOST).
A sound knowledge regarding handling of laboratory equipment such as spectrophotometer, centrifuge and other basic
instrumentation.
Good laboratory practices when it comes to Microbiology based research.
A very good knowledge about the basic aspects of Biochemistry, Microbiology, Genetics, Genetic engineering,
Biochemical process principles and Industrial aspects of Biotechnology.
I have been able to gain enough knowledge about techniques such as High Pressure Liquid Chromatography and
Polymerase Chain Reactors through the many workshops I have been able to attend.
CAREER CONTOUR
Since January ‟12 till March ‟12 as In Plant trainee
PROFESSIONAL EXPERIENCE:
Client Name: Creamline Dairy Products limited
Project : Production and Quality control of milk and milk by products
Responsibilities:
Studying the production aspects of milk and it‟s by products.
Monitoring the mechanisms of the Pasteurizers, Standardization of milk, Continuous Butter Making Machine, Packing
machines, Cottage cheese production, Ice Cream production, Curd production, Dhoodh Peda production and Flavoured
milk production.
Studying the quality determining methods for milk, Butter, Ghee, Cottage cheese, Ice Cream, Curd, Dhoodh peda and
Flavoured milk.
The quality determining methods includes adulterants detection, fat percentage, Solids-not-fat percentage, acidity,
microbial testing in the raw cum final product and quality aspects of the product packaging.
Monitoring the Good Manufacturing Practises (GMP) practised in the large scale industry.
Need for following the GMPs.
Study of the functions and mechanisms of the only “Effluent Treatment Plant” owned by a large scale plant.
Study of the machineries used in the milk industry.
Since November ‟12 till May ‟13 Trainee Study Personnel
Client Name: RCC Laboratories Pvt. Ltd.
Project : Pre Clinical evaluation-Genetic Toxicology studies (invitro studies)
Responsibilities:
Knowledgeable in the principles of GLP which is the core and primary basis of the studies conducted.
Assist the Study Director in preparing study plans, perform studies and record the raw data as per the study plan and
Standard Operating Procedures (SOP).
Microbiological monitoring of animal facilities and working areas as required.
Handling animals for in vivo studies.
2. Update, maintain knowledge about the recent advances in the areas related to the study and share the same with the team
on a continuous basis.
Participate in study and related meetings and ensure the up gradation of procedures and performances of the study.
Plan, discuss study plans with study team members and work in collaboration to achieve desired results.
Check that the required amount of test items are available to carry out the studies,
Prepare and review SOPs in respective areas of responsibility.
Interface with the Quality Assurance Unit (QAU) as required and liaison with the QA staff and scientific and technical
personnel to facilitate responses to inspection/ audit findings.
Indenting and initiating the process of procurement by technical evaluation and preliminary discussion of lab chemicals,
consumables and other equipments/ instruments necessary to carry out the studies.
To assist the Study Director in processing and screening slides and perform post mortem in case of in vivo studies.
Calibration of the equipments and handling of instruments as per the user manual and SOP.
Manage the studies in both accuracy and punctuality as a team.
Since September ‟14 till September „15
Client Name: Indian Institute of Chemical Technology, Hyderabad
Project : Process optimization for Candida antarctica lipase B production in submerged batch fermentation
through classical design of experiments
Skills, roles and Responsibilities:
Maintaining culture viability through regular sub-culturing.
Media preparation, component optimization and proper disposal after use.
Handling and maintenance of 3L automated fermenter, UV/visible spectrophotometer, microscope, weighing balance,
magnetic stirrer with/without heating, sonicator, incubator, cooling centrifuge, autoclave, laminar air flow and
homogeniser.
Substrate assays, media and appropriate instrument autoclaving.
Preparing weekly reports, supervising purchase, servicing, installation and maintenance of equipments.
Assisting the scientist in preparing experimental schedule, perform studies and record and analyze the results.
EXTRA CURRICULARS
Authored oral paper on “ Microbial growth kinetics for Candida antarctica lipase B production in submerged
fermentation” as a part of CHEMCON 2015, a 68th Annual Session of the Indian Institute of Chemical Engineers at IIT
Guwahati.
I have presented an oral paper on “Kinetic evaluation of microbial growth implicated in lipase production” and co-
authored “Kinetic study on enzymatic hydrolysis of tributyrin” at NIT, Warangal as a part of International
Conference on New Frontiers in Chemical, Energy and Environmental Engineering 2015 (INCEEE 2015) which was
organized on the occasion of 50th
anniversary of Chemical Engineering‟s existence at NIT, Warangal.
Presented poster at Indo-US Conference on Advanced Lignocellulosic Biofuels, 2014 at IICT (Hyderabad) on “Recent
advances on Biomass Pre-treatment methods”.
Attended workshop on “Intellectual Property Rights” conducted by Patent Information Centre (PIC), A.P State
Council of Science and Technology (APCOST).
Attended workshop on “Opportunities for Indo-European Research & Innovation cooperation” conducted in light of
HORIZON 2020, conducted by EU-India, European Union Delegation to India.
The article I wrote on “Regeneration of Human Organ by Nanoengineering” was published in a renowned Biotech online
magazine called BIOSAINT
(link: http://www.biosaints.com/archives/vol1/vol1_2/regenerative_medicine.html)
My thesis on diseases spread through contamination of milk and how they affect the Food industry which is partially
based on my major project has been published in the September 2012 issue of an international online magazine “GERMS”
(link: www.germs.ro)
I have been also considered for the position of a reviewer for some of the thesis submitted online.
I have scored a rank of 663 in GATE 2011 and 938 in GATE 2013 in All India level (AIR).
I was the student coordinator for the national level technical fest conducted in our institution called “AAYUKTHA”.
I have presented many papers presentations in various prestigious institutions and secured second position in two of them.
I have participated in college level Throw ball competition and have managed to secure the first position, as with our
team.
I have participated and have won many competitions such as in Essay writing, Drawing and Elocutions.
My active participations in many sports such as in Volley ball, Shot put, Kabbadi, Caroms and Throw ball has won me
many accolades.
Participated in “RUN FOR WOMEN 2K12” emphasizing the importance of physical exercise to women.
Volunteered as teacher in TEACH FOR INDIA.
3. ACADEMIC CREDENTIALS
M.Tech in Biochemical Engineering and Biotechnology
Osmania University College of Technology (2013-2015) with an aggregate of 93.45%
B.Tech. in Biotechnology
JNTU (Hyderabad) (2008-2012) with 82.36%.
Class XII from Narayana Junior College, Habsiguda, Hyderabad in 2008 with 86.6%.
(State Board of Intermediate Education)
Class X from Johnson Grammar, Habsiguda, Hyderabad in 2006 with 83.83%.
ACADEMIC PROJECT
Organisation : Indian Institute of Chemical Technology
Title : Process optimization for Candida antarctica lipase B production in submerged batch fermentation
through classical design of experiments
Period : Sept „14- Sept „15
Environment : Laboratory Exposure
Description : Study of the effects and optimization of the various operating parameters on lipase production form
Candida antarctica
Organisation : Creamline Dairy Products limited
Title : Study of Production and Quality Control of milk and milk based products
Period : Jan‟12 – Mar‟12
Environment : On Site training
Description : Study of every aspect related to the production and processing of milk unto its final product.
IT FORTE
Software : Bioinformatics tools such as BLAST, FASTA, Multiple Sequence Alignment, Data Mining,
Mega5, BioEdit, RASMOL/RASWIN etc.
Others : Microsoft office, Adobe photoshop.
PERSONAL DOSSIER
Date of Birth : 11th
August, 1990
Linguistic Abilities : English, Hindi, Telugu and Tamil.
Residential Address : H No: 5-3/2/26, Plot No: 14, Padmavathi Colony, Boduppal, Hyderabad – 500092
DECLARATION:
I hereby declare that all the above-mentioned information is correct as per my knowledge and I bear the responsibility for the
correctness of the above-mentioned particulars.
Place
Hyderabad. (D.Revathi)