This study investigated the relationship between vocabulary size and reading comprehension ability in 30 Thai first-year university students studying English. The students completed vocabulary level tests assessing knowledge of words at the 2000, 3000 and 5000 frequency levels, as well as a reading comprehension test. Results showed the students' vocabulary size decreased as the word frequency levels increased. Vocabulary size at all three levels had a significant positive correlation with reading comprehension ability, with stronger correlations at higher frequency levels. The findings suggest vocabulary instruction should be a key part of English courses to help students build the vocabulary knowledge needed for effective reading.