The document describes using an automated high-throughput phenotyping platform called PlantScreenTM Conveyer System to visualize and quantify the early stress response of Arabidopsis thaliana plants to the herbicide glyphosate. RGB imaging, chlorophyll fluorescence kinetics imaging, and hyperspectral imaging were used to analyze plant structure, growth, and photosynthetic performance over 72 hours after glyphosate application. Results showed glyphosate impaired growth dynamics after 20 hours and decreased chlorophyll content and photosynthetic performance within 10-13 hours, as indicated by changes in fluorescence parameters, NDVI, and other indices. The study demonstrated how high-throughput phenotyping can help characterize plant stress responses and accelerate crop breeding.