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The Protein Efficiency Ratio (PER) is defined as the weight gain of the test group divided by the protein consumed by the test group. The advantages of PER are that only simple equipment like rat cages and balances are needed. However, PER only measures growth and maintenance requirements together, not separately, and cannot distinguish between weight gained as fat or lean body mass. When using PER, rats that are 21 days weaned are the typical animal tested using a 10% protein concentration diet compared to ovalbumin over a 3 week feeding trial while recording weight gained and food consumed.


