This document describes various cell culture products from Promega for quantifying live and dead cells, assessing apoptosis, and measuring cellular metabolism. It lists assays for quantifying live cells using luminescent or fluorescent methods. Assays are also listed for quantifying dead cells by measuring protease or LDH activity leaked from cells with compromised membranes. Additional assays allow assessing apoptosis by measuring caspase activity or DNA fragmentation. Further assays measure changes in glutathione levels or proteasome and cytochrome P450 activity to analyze cellular metabolism. Contact information is provided for Whitehead Scientific suppliers in various South African regions.

1. WhiteSci
Whitehead Scientific
Buying Cell Culture Equipment or Media?
Consider these Promega Companion Products
Quantitate Changes in the number of Dead Cells
™
• CytoTox-Glo Cytotoxicity Assay
™
• CytoTox-Fluor Cytotoxicity Assay
™
• CytoTox-ONE Homogeneous Membrane
Integrity Assay
Assess whether these cells die from Apoptosis
• Caspase-Glo® Assays
®
• ApoONE Homogeneous Caspase 3/7 Assay
™
• DeadEnd TUNEL Assays Biological
™
• CaspACE FITC-VAD-FMK in Situ Marker Safety Hoods
Treat Cells &
Assess Biological Effect
Cell Culture
Quantitate Changes in the number of Live Cells
• CellTiter-Glo® Luminescent Cell Viability Assay
• CellTiter-Fluor™ Cell Viability Assay
®
• CellTiter-Blue Cell Viability Assay
• CellTiter 96® AQueous One Solution Assay
Assess changes in cellular Metabolism
• GSH-Glo™ Glutathione Assay
™
• Proteasome-Glo Assays
™
• P450-Glo Cell Based Assays Co2 Incubators
Western & Eastern Cape Gauteng, KZN, Free State & Limpopo
TEL (021) 944 6460, FAX (021) 949 5478 TEL (011) 894 2214, FAX (011) 894 4583
e-mail penny@whitesci.co.za e-mail luzaan@whitesci.co.za
www.whitesci.co.za
P.T.O

2. Promega Companion Products for your cell culture work
Quantitate Changes in the number of Live Cells Quantitate Changes in the number of Dead Cells
Description Size Cat. # Description Size Cat. #
10ml G7570 CytoTox-Glo™ Cytotoxicity Assay 10ml G9290
Luminescent
CellTiter-Glo® Luminescent Cell Viability Assay 10 x 10ml G7571 protease assay measures “dead cell” protease 5 x 10ml G9291
Quick 10 minute luminescent ATP assay with leaked into culture media from cells with
sensitivity down to the 10’s of cells. Scalable to compromised membranes. 2 x 50ml G9292
96-, 384- and 1536-well plate formats. 100ml G7572
CytoTox-Fluor™ Cytotoxicity Assay 10ml G9260
10 x 100ml G7573 Fluorescent
(Rhodamine 110: 485nmEx/520nmEm) protease 5 x 10ml G9261
CellTiter-Fluor™ Cell Viability Assay 10ml G6080 assay measures “dead cell” protease leaked into
Non-lytic culture media from cells with compromised
fluorescent assay membranes. 2 x 50ml G9262
(AFC; 400nmEx/505nmEm) with 5 x 10ml G6081
sensitivity down to the 100’s of cells. Excellent
choice for multiplexing with bioluminescent assays. CytoTox-ONE™ Homogeneous Membrane 200-800
G7890
Scalable to 96-, 384- and 1536-well plates. 2 x 50ml G6082 Integrity Assay assays
Fluorescent (Resorufin;
560nmEx/590nmEm) assay of LDH activity leaked 1000-4000
20ml G8080 G7891
from cells with compromised membranes. assays
CellTiter-Blue® Cell Viability Assay
Resazurin-based, non-lytic fluorescent assay
100ml G8081
(resorufin; 560nmEx/590nmEm) with sensitivity down Assess whether the cells die from Apoptosis
to the 100’s of cells.
10 x 100ml G8082 2.5ml G8090
CellTiter 96® AQueous One Solution 200 assay G3582 Caspase-Glo® 3/7 Assay 10ml G8091
Cell Luminescent protease
Proliferation Assay assay measuring the cleavage of DEVD-luciferin. 10 x 10ml G8093
1000 assay G3580
MTS-based, non-lytic
colorimetric assay (Absorbance 490nm) with 100ml G8092
sensitivity down to the 1000’s of cells. 5000 assay G3581
2.5ml G8200
Assess changes in Cellular Metabolism Caspase-Glo® 8 Assay
Luminescent protease 10ml G8201
GSH-Glo™ Glutathione Assay assay measuring the cleavage of LETD-luciferin
10ml V6911
Cell-based assay to 100ml G8202
understand oxidative stress through glutathione
levels. Simple protocol does not require removal of 50ml V6912 2.5ml G8210
proteins.
Caspase-Glo® 9 Assay
10ml G8660 Luminescent protease 10ml G8211
Proteasome-Glo™ Chymotrypsin-like Cell Based assay measuring the cleavage of LEHD-luciferin.
Assay
Homogeneous luminescent protease assay 100ml G8212
for assessing the chymotrypsin-like activity of the 5 x 10ml G8661
26S proteosome. 1ml G7792
Apo-ONE® Homogeneous
Caspase 3/7 Assay
Proteasome-Glo™ Trypsin-Like Cell Based 10ml G8760 Fluorescent ((Rhodamine 110: 10ml G7790
Assay 485nmEx/520nmEm) protease assay measuring the
Homogeneous luminescent protease assay cleavage of DEVD substrate.
for assessing the trypsin-like activity of the 26S 100ml G7791
5 x 10ml G8761
proteosome.
DeadEnd™ Fluorometric TUNEL System 60 rxn G3250
10ml G8860 Classic TUNEL kit with fluorescein dUTP.
Proteasome-Glo ™ Caspase-like Cell Based
Assay
Homogeneous luminescent protease assay DeadEnd™ Colorimetric TUNEL System
for assessing the caspase-like activity of the 26S Classic 40 rxn G7130
5 x 10ml G8861 TUNEL kit with biotinylated dUTP and
proteosome.
streptavidin horseradish peroxidase.
10 ml G1180 CaspACE™ FITC-VAD-FMK 50µl G7461
Proteasome-Glo ™ 3-Substrate Cell Based
Assay In Situ Marker
Homogeneous luminescent protease assay Simply add to live cells to label active caspases.
Applications to flow cytometry. 125µl G7462
for assessing the 3-substrate activity of the 26S 50ml G1200
proteosome.
CaspACE, CellTiter-Fluor, CytoTox-Fluor, CytoTox-Glo, CytoTox-ONE, DeadEnd, GSH-Glo and Proteasome-Glo are
trademarks; Apo-ONE, Caspase-Glo, CellTiter 96, CellTiter-Blue, and CellTiter-Glo are registered trademarks of Promega
P450-Glo™ CYP3A4 Cell Based Assay 10ml V8901 Corporation. Products may be covered by existing or pending patents, please visit www.promega.com for more information.
Simple
add-mix-measure assay to assess inhibition or
induction of cytochrome p450 3A4. 50ml V6912 Learn more about the latest products and applications for all
your research needs at www.promega.com
Western & Eastern Cape Gauteng, KZN, Free State & Limpopo
TEL (021) 944 6460, FAX (021) 949 5478 TEL (011) 894 2214, FAX (011) 894 4583
e-mail penny@whitesci.co.za e-mail luzaan@whitesci.co.za
www.whitesci.co.za