This study evaluated the semen characteristics of three banteng (Bos javanicus) bulls using electroejaculation. Semen was collected from two bulls at Taman Safari Indonesia and one bull at Ragunan Zoo. The semen was evaluated for volume, color, consistency, pH, sperm motility, viability, concentration and morphology. The semen volume ranged from 4-7.4 ml. Color varied from dirty white to milky white to cream. Consistency was watery to moderately thick. pH ranged from 6.4-6.8. Sperm motility, viability and normal morphology also varied between samples. This study provided additional data on banteng semen characteristics collected via electro
Combining Metabolite-Based Pharmacophores with Bayesian Machine Learning Mode...Sean Ekins
Slides from SERMACS 2015 meeting in Memphis 2015 describing a collaborative project with SRI International and Rutgers. The work was published in PLOS ONE http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141076
Potential for genomic selection in indigenous cattle breeds and results of GWAS in Gir dairy cattle of Gujrat by Dr.Pravin Kandhani and Dr. Vijay Trivedi KAMDHENU UNIVERSITY GANDHINAGAR
Effect of Adlai (Coix lacryma-jobi L.) roots on small ruminants naturally inf...Innspub Net
Parasites pose a significant threat to the health of small ruminants. Natural products such as Adlai roots have anthelmintic potentials. The study aimed to determine the Anthelmintic potential to the small ruminants. A total of 12 goats and sheep regardless of sex and age were used in the study and randomly distributed into four (4) Treatments with Three (3) replications following Randomized Complete Block Design. There were four different levels of Treatments. Treatment 1 as the control, Treatment 2 (100ml of Adlai roots decoction), Treatment 3 (150ml of Adlai roots decoction), and Treatment 4 (200ml of Adlai roots decoction). Freshly excreted feces were collected and placed in plastic cups and immediately examined through Modified Mc Master Test to evaluate the Anthelmintic activity of the designated treatments. The evaluation criteria were based on the frequency counts of egg parasites and oocysts per 2 grams of feces under different treatments. Results were analyzed using the analysis of variance (ANOVA) of Randomized Complete Block Design (RCBD). Tukey’s test was used to compare treatment means showing significant difference. Statistical results were non-significant among Treatment means in all of the parameters except for the strongylid counts for the month of April which showed significant difference among treatment mean at (P< 0.05) where sheep treated with Treatment 4 (200ml of Adlai roots decoction). Adlai roots decoction has anti-parasitic activity.
Combining Metabolite-Based Pharmacophores with Bayesian Machine Learning Mode...Sean Ekins
Slides from SERMACS 2015 meeting in Memphis 2015 describing a collaborative project with SRI International and Rutgers. The work was published in PLOS ONE http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0141076
Potential for genomic selection in indigenous cattle breeds and results of GWAS in Gir dairy cattle of Gujrat by Dr.Pravin Kandhani and Dr. Vijay Trivedi KAMDHENU UNIVERSITY GANDHINAGAR
Effect of Adlai (Coix lacryma-jobi L.) roots on small ruminants naturally inf...Innspub Net
Parasites pose a significant threat to the health of small ruminants. Natural products such as Adlai roots have anthelmintic potentials. The study aimed to determine the Anthelmintic potential to the small ruminants. A total of 12 goats and sheep regardless of sex and age were used in the study and randomly distributed into four (4) Treatments with Three (3) replications following Randomized Complete Block Design. There were four different levels of Treatments. Treatment 1 as the control, Treatment 2 (100ml of Adlai roots decoction), Treatment 3 (150ml of Adlai roots decoction), and Treatment 4 (200ml of Adlai roots decoction). Freshly excreted feces were collected and placed in plastic cups and immediately examined through Modified Mc Master Test to evaluate the Anthelmintic activity of the designated treatments. The evaluation criteria were based on the frequency counts of egg parasites and oocysts per 2 grams of feces under different treatments. Results were analyzed using the analysis of variance (ANOVA) of Randomized Complete Block Design (RCBD). Tukey’s test was used to compare treatment means showing significant difference. Statistical results were non-significant among Treatment means in all of the parameters except for the strongylid counts for the month of April which showed significant difference among treatment mean at (P< 0.05) where sheep treated with Treatment 4 (200ml of Adlai roots decoction). Adlai roots decoction has anti-parasitic activity.
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
Fertility Response Following Induction of Lactation in Infertile Dairy Cowsijtsrd
The fertility response following induction protocol in infertile dry cows was assessed in terms of the induction of oestrus in anoestrus cows and conception in repeat breeders cows in the study. There were four anoestrous and two repeat breeder cows in G-1 out of them two anoestrus became cyclic, one repeater animal conceived after treatment protocol. Where as in G-2, three anoestrus and three repeat breeder animals given induction protocol where two anoestrous cows became cyclic and two repeaters conceived. The analysis of data revealed higher fertility response in G-2 as compared to G-1 (66.67 Vs. 50 %, respectively) with the higher conception rate (50 Vs. 33.33 %, respectively). It indicates better fertility response in G-2 as compared to G-1. K. Kumar | S. N. Shukla | S. Bhandekar | S. K. Singh | P. Inwati"Fertility Response Following Induction of Lactation in Infertile Dairy Cows" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd9615.pdf http://www.ijtsrd.com/other-scientific-research-area/other/9615/fertility-response-following-induction-of-lactation-in-infertile-dairy-cows/k-kumar
Reversible Antifertility Effect of Cassia tora Linn in Male Rats
Samiya Khan, Pratap Chand Mali*
*Address for Correspondence: Dr. Pratap Chand Mali, Associate Professor, Reproductive Biomedicine and Natural Products Lab, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India
ABSTRACT- Background: Plant Cassia tora has been used in traditional and modern medicines for different pharmacological activities.
Objectives: The present investigation has been taken to observe and evaluate effects of Cassia tora on the reproduction functions of male rats in search a safe, orally effective and reversible fertility regulating agent.
Materials and Methods: Fifty percent ethanolic extract of Cassia tora was prepared and administered orally in male Wistar rats at the doses of 50, 100 and 200 mg/kg.b.wt./rat/day dose levels respectively for a period of 60 days and some of the treated rats were kept 30 days for recovery of fertility to assessed reversibility effects. Hematological indices, serum clinical investigations were also performed to assess toxic effects if any caused in rats by treatment. Proteins, cholesterol, glycogen, ascorbic acid, sialic acid and fructose level were analyzed in rats. Serum FSH, LH and Testosterone levels were measure. Rats were castrated to evaluate effects on reproductive functions of hormones and mode of action of the Cassia tora treatment. For histopathological observations tissues were fixed in Bouin’s fluid, dehydrated, sectioned and stained with Hematoxylin and Eosin.
Results: Treatment of Cassia tora significantly reduced the weights of testes and accessory sex organs. Sperm density and motility were declined high significantly. Levels of Testosterone and FSH hormone were significantly decreased in rats. The protein, sialic acid, fructose, ascorbic acid and glycogen contents of reproductive accessory sex organs were decreased significantly. Germinal epithelium of testes degenerated and number of spermatocytes, spermatids and spermatozoa in lumen of seminiferous tubules reduced.
Conclusions: The decreased testes and accessory sex organs weights, sperm motility, density and testosterone level in rats might be due to androgen suppression effects of Cassia tora treatment cause inhibition of spermatogenesis resulted reduction of fertility in treated male rats.
Key-words- Cassia tora, Contraception, Fertility, Sperm motility, Sperm density, Male rat
The relationship between trace mineral concentrations of amniotic fluid with ...Ali Olfati
Ali Olfati1*, Gholamali Moghaddam1, Nasroallah Moradi Kor2, Behzad Baradaran3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran
3Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Evaluation of some reproduction performances of the exotic Hyplus rabbit (Ory...Open Access Research Paper
The objective of the study was to evaluate the reproductive performance and estimate the genetic parameters, in a real environment, of the rabbit of the exotic Hyplus breed. The study will allow appreciating the parameters that could be the object of genetic improvement in view of a selection to produce a more efficient rabbit. The work took place at the SAP Mé d’Adzopé farm from January 2013 to June 2015. One hundred and twenty breeding does were followed for five successive generations of five parities each, with an interval of 11 days between the parturition and the next mating with weaning at 30 days. These females produced 5283 total born rabbits with 5164 born alive and 4732 weaned. The genetic parameters including heritability, genetic, phenotypic and non-genetic correlations (environmental effects) did analyzed using the Derivative Free Restricted Maximum Likelihood program. The results obtained showed that the receptivity and fertility rates did significantly influenced by the order of parity and the season of kindling. The pre-weaning mortality rate was 8.37%. Females had the best prolificacies’ between the second and fourth parity, with an average of 9.9, 10.19 and 9.79 kits respectively. The heritability values of young rabbits total born alive, born alive, weaned and old rabbits were 0.22; 0.20; 0.16 and 0.12 respectively. These results showed that selection is possible for these studied traits.
Serological evidence of brucellosis in goatsSameer Sankhe
This ppt is related to the serological evidence of brucellosis in goats. Brucellosis is an important zoonotic disease of public health and veterinary sector
Genome wide association study and factors affecting semen volume and concentr...ILRI
poster prepared by Selam Meseret, Seid Mohammed, Tegegn Fantahun, Genet Zewdie, Megersa Abone, Genet Dejene, Raphael Mrode and Okeyo A. Mwai for the 29th Ethiopian Society of Animal Production (ESAP) Conference, Addis Ababa, 28-30 October 2021
The relationship between progesterone and biochemical constituents of amnioti...Ali Olfati
Ali Olfati1, Gholamali Moghaddam1, Nasroallah Moradi Kor2*, Mitra Bakhtiari3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Iran
2Department of Reproduction Physiologies, Iranian Society of Physiology and Pharmacology, Tehran, Iran
3Department of Anatomical Sciences, Faculty of Medicine, University of Medical Sciences, Kermanshah, Iran
Fertility Response Following Induction of Lactation in Infertile Dairy Cowsijtsrd
The fertility response following induction protocol in infertile dry cows was assessed in terms of the induction of oestrus in anoestrus cows and conception in repeat breeders cows in the study. There were four anoestrous and two repeat breeder cows in G-1 out of them two anoestrus became cyclic, one repeater animal conceived after treatment protocol. Where as in G-2, three anoestrus and three repeat breeder animals given induction protocol where two anoestrous cows became cyclic and two repeaters conceived. The analysis of data revealed higher fertility response in G-2 as compared to G-1 (66.67 Vs. 50 %, respectively) with the higher conception rate (50 Vs. 33.33 %, respectively). It indicates better fertility response in G-2 as compared to G-1. K. Kumar | S. N. Shukla | S. Bhandekar | S. K. Singh | P. Inwati"Fertility Response Following Induction of Lactation in Infertile Dairy Cows" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-2 , February 2018, URL: http://www.ijtsrd.com/papers/ijtsrd9615.pdf http://www.ijtsrd.com/other-scientific-research-area/other/9615/fertility-response-following-induction-of-lactation-in-infertile-dairy-cows/k-kumar
Reversible Antifertility Effect of Cassia tora Linn in Male Rats
Samiya Khan, Pratap Chand Mali*
*Address for Correspondence: Dr. Pratap Chand Mali, Associate Professor, Reproductive Biomedicine and Natural Products Lab, Centre for Advanced Studies, Department of Zoology, University of Rajasthan, Jaipur, India
ABSTRACT- Background: Plant Cassia tora has been used in traditional and modern medicines for different pharmacological activities.
Objectives: The present investigation has been taken to observe and evaluate effects of Cassia tora on the reproduction functions of male rats in search a safe, orally effective and reversible fertility regulating agent.
Materials and Methods: Fifty percent ethanolic extract of Cassia tora was prepared and administered orally in male Wistar rats at the doses of 50, 100 and 200 mg/kg.b.wt./rat/day dose levels respectively for a period of 60 days and some of the treated rats were kept 30 days for recovery of fertility to assessed reversibility effects. Hematological indices, serum clinical investigations were also performed to assess toxic effects if any caused in rats by treatment. Proteins, cholesterol, glycogen, ascorbic acid, sialic acid and fructose level were analyzed in rats. Serum FSH, LH and Testosterone levels were measure. Rats were castrated to evaluate effects on reproductive functions of hormones and mode of action of the Cassia tora treatment. For histopathological observations tissues were fixed in Bouin’s fluid, dehydrated, sectioned and stained with Hematoxylin and Eosin.
Results: Treatment of Cassia tora significantly reduced the weights of testes and accessory sex organs. Sperm density and motility were declined high significantly. Levels of Testosterone and FSH hormone were significantly decreased in rats. The protein, sialic acid, fructose, ascorbic acid and glycogen contents of reproductive accessory sex organs were decreased significantly. Germinal epithelium of testes degenerated and number of spermatocytes, spermatids and spermatozoa in lumen of seminiferous tubules reduced.
Conclusions: The decreased testes and accessory sex organs weights, sperm motility, density and testosterone level in rats might be due to androgen suppression effects of Cassia tora treatment cause inhibition of spermatogenesis resulted reduction of fertility in treated male rats.
Key-words- Cassia tora, Contraception, Fertility, Sperm motility, Sperm density, Male rat
The relationship between trace mineral concentrations of amniotic fluid with ...Ali Olfati
Ali Olfati1*, Gholamali Moghaddam1, Nasroallah Moradi Kor2, Behzad Baradaran3
1Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
2Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran
3Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Evaluation of some reproduction performances of the exotic Hyplus rabbit (Ory...Open Access Research Paper
The objective of the study was to evaluate the reproductive performance and estimate the genetic parameters, in a real environment, of the rabbit of the exotic Hyplus breed. The study will allow appreciating the parameters that could be the object of genetic improvement in view of a selection to produce a more efficient rabbit. The work took place at the SAP Mé d’Adzopé farm from January 2013 to June 2015. One hundred and twenty breeding does were followed for five successive generations of five parities each, with an interval of 11 days between the parturition and the next mating with weaning at 30 days. These females produced 5283 total born rabbits with 5164 born alive and 4732 weaned. The genetic parameters including heritability, genetic, phenotypic and non-genetic correlations (environmental effects) did analyzed using the Derivative Free Restricted Maximum Likelihood program. The results obtained showed that the receptivity and fertility rates did significantly influenced by the order of parity and the season of kindling. The pre-weaning mortality rate was 8.37%. Females had the best prolificacies’ between the second and fourth parity, with an average of 9.9, 10.19 and 9.79 kits respectively. The heritability values of young rabbits total born alive, born alive, weaned and old rabbits were 0.22; 0.20; 0.16 and 0.12 respectively. These results showed that selection is possible for these studied traits.
Serological evidence of brucellosis in goatsSameer Sankhe
This ppt is related to the serological evidence of brucellosis in goats. Brucellosis is an important zoonotic disease of public health and veterinary sector
Genome wide association study and factors affecting semen volume and concentr...ILRI
poster prepared by Selam Meseret, Seid Mohammed, Tegegn Fantahun, Genet Zewdie, Megersa Abone, Genet Dejene, Raphael Mrode and Okeyo A. Mwai for the 29th Ethiopian Society of Animal Production (ESAP) Conference, Addis Ababa, 28-30 October 2021
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Cancer cell metabolism: special Reference to Lactate PathwayAADYARAJPANDEY1
Normal Cell Metabolism:
Cellular respiration describes the series of steps that cells use to break down sugar and other chemicals to get the energy we need to function.
Energy is stored in the bonds of glucose and when glucose is broken down, much of that energy is released.
Cell utilize energy in the form of ATP.
The first step of respiration is called glycolysis. In a series of steps, glycolysis breaks glucose into two smaller molecules - a chemical called pyruvate. A small amount of ATP is formed during this process.
Most healthy cells continue the breakdown in a second process, called the Kreb's cycle. The Kreb's cycle allows cells to “burn” the pyruvates made in glycolysis to get more ATP.
The last step in the breakdown of glucose is called oxidative phosphorylation (Ox-Phos).
It takes place in specialized cell structures called mitochondria. This process produces a large amount of ATP. Importantly, cells need oxygen to complete oxidative phosphorylation.
If a cell completes only glycolysis, only 2 molecules of ATP are made per glucose. However, if the cell completes the entire respiration process (glycolysis - Kreb's - oxidative phosphorylation), about 36 molecules of ATP are created, giving it much more energy to use.
IN CANCER CELL:
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
Unlike healthy cells that "burn" the entire molecule of sugar to capture a large amount of energy as ATP, cancer cells are wasteful.
Cancer cells only partially break down sugar molecules. They overuse the first step of respiration, glycolysis. They frequently do not complete the second step, oxidative phosphorylation.
This results in only 2 molecules of ATP per each glucose molecule instead of the 36 or so ATPs healthy cells gain. As a result, cancer cells need to use a lot more sugar molecules to get enough energy to survive.
introduction to WARBERG PHENOMENA:
WARBURG EFFECT Usually, cancer cells are highly glycolytic (glucose addiction) and take up more glucose than do normal cells from outside.
Otto Heinrich Warburg (; 8 October 1883 – 1 August 1970) In 1931 was awarded the Nobel Prize in Physiology for his "discovery of the nature and mode of action of the respiratory enzyme.
WARNBURG EFFECT : cancer cells under aerobic (well-oxygenated) conditions to metabolize glucose to lactate (aerobic glycolysis) is known as the Warburg effect. Warburg made the observation that tumor slices consume glucose and secrete lactate at a higher rate than normal tissues.
Cancer cell metabolism: special Reference to Lactate Pathway
Proceeding of FAVA: Semen Characteristics of Banteng (Bos Javanicus) Collected by Electroejaculation
1. Proc. of the 20th FAVA CONGRESS & The 15thKIVNAS PDHI, Bali Nov 1-3, 2018 | 61
Oral Presentation (FA-5)
Semen Characteristics of Banteng (Bos javanicus) Collected by Electroejaculation
Method
Vincentia Trisna Yoelinda1, Iis Arifiantini2, Muhammad Agil2*,Dedi R. Setiadi2, Tuty L. Yusuf2, Yohana Tri
Hastuti3, Jansen Manansang3 Dondin Sajuthi2
1Study Program of Reproductive Biology, Graduate School of Bogor Agricultural University,
West Java 16680, Indonesia
2Department of Veterinary Clinic, Reproduction and Pathology, Faculty of Veterinary Medicine,
Bogor Agricultural University, West Java 16680, Indonesia
3Taman Safari Indonesia, Cisarua, Bogor
*Corresponding author’s email: rhinogil@googlemail.com
Keywords: banteng, semen, electroejaculation, artificial insemination.
INTRODUCTION
Banteng (Bos javanicus) is listed as
endangered species in IUCN Redlist. Only few
thousand wild banteng survive and their numbers
are decreasing as a result of hunting and the loss of
habitat due to deforestation and conversion to
agricultural land. One of the conservation
measures is to preserve this species in ex-situ
breeding center. The breeding centre with the
largest number of individuals is Ragunan Zoo in
Jakarta, with 21 animals. Despite those numbers of
individuals available, the sex ratio was far from
ideal at 16 males and five females. While in Taman
Safari Prigen, there were with 16 individuals and a
sex ratio of five males and 11 females [1] Since
number of males are usually less than females, and
also to avoid inbreeding in order to keep high
heterozygosity population, therefore, assisted
reproductive technology such as artificial
insemination is considered to be necessary.
For artificial insemination, semen
collection sufficient quantitiy and quality of semen
from males are needed. Thus, best semen collection
method should be considered. Though transrectal
massage procedure does not require the male to be
anesthetized prior to semen collection, semen
collected by this technique contains urine
contamination. Besides, volume of semen sample
obtained by transrectal massage is lesser
compared to electroejaculation. Electroejaculation
is also suitable for wild animal because in this
particular semen collection procedure, males are
not required to be trained.
The information about semen
characteristics of banteng bulls collected by
electroejaculation is less known. To date, study
done by Johnston et al. (2002) is the only available
data. That study only provided data from one
semen sample from one 4-year-old banteng bull
located at Western Plains Zoo [2]. Moreover such
volume parameter and consistency was not
reported from that study. Thus, this study is aimed
to provide more reliable data from larger number
of semen samples collected by electroejaculation
and to add information to previous data that are
availabe.
MATERIALS AND METHODS
Animas and Husbandry
The study was undertaken at Taman Safari
Indonesia Cisarua, Bogor, Indonesia and Ragunan
Zoo, Jakarta, Indonesia. The study used two
banteng bulls (B#1 and B#2) at Taman Safari
Indonesia, Cisarua, Bogor and one bull (B#3)
atRagunan Zoo. Four ejaculates samples (S-1B#1,
S-2B#2, S-3B#3, S-4B#3) were collected from 3
banteng (Bos javanicus) with age of 4-15 years old
and body weights of 400-600 kg.
Semen Collection
Prior to semen collection, the bulls were
anesthetized by the authorized zoo veterinarian
using combination of ketamine (1 mg/kg) and
xylazine (0,5 mg/kg). As bulls were anesthetized,
they were restrained in lateral recumbency.
Immediately, after the bulls were anesthetized
preputial hairs were clipped. The preputial area
was then washed with soap, rinsed with clean
water and rubbed with towel. Inner portion of the
preputium was flushed with sterile physiological
saline, then rubbed gently with paper towel. Semen
collection was performed by the authorized
veterinarian using electroejaculator immediately
after anesthesia. Electroejaculator rectal probe
was lubricated then inserted into the anus until 20
cm in depth and directed against the pelvic floor.
Each stimulus lasted for 5 s and was followed by a
pause of 5 s. The stimulus was repeated until penile
erection appeared followed by ejaculation.
Electroejaculation was attempted once per
banteng bull, except one banteng bull at Ragunan
Zoo, the electroejaculation was attempted twice
with one month of interval.
2. 62 | Proc. of the 20th FAVA CONGRESS & The 15thKIVNAS PDHI, Bali Nov 1-3, 2018
Semen Evaluation
Ejaculates were evaluated immediately
after collection. Four ejaculates collected from 3
banteng bulls were evaluated either
macroscopically and microscopically.
Macroscopically, semen samples were
evaluated for volume, consistency, color and pH.
The semen volume was measured by reading the
scale on collection tube, the colour assessed by eye.
Semen pH value was examined using pH indicator
paper (Merck, Germany). Microscopic parameter of
semen characteristics were assessed subjectively.
Semen samples were evaluated for progressive
motility, velocity, viability, sperm concentration
and precentage of morphologically normal
spermatozoa. Progresive motility was subjectively
assessed by placing semen sample with
physiological saline on glass slide. The percentage
of live sperm and the percentage of
morphologically normal spermatozoa were
determined using eosin-negrosin staining. Sperm
concentration was determined by calculating
spermatozoa per 1 mililiter semen, counted using
Neubauer chamber.
RESULT AND DISCUSSION
Electroejaculation was successfully done
in 2 banteng bulls at Taman Safari Prigen,
Indonesia and one banteng bull at Ragunan Zoo,
Indonesia (Figure 1). The result of macroscopic
and microscopic evaluation of semen obtained are
shown in Table 1. Semen samples collected by
electroejaculation showed various results which
were dirty white, milky white, and cream in colour.
This finding agreed with Johnston et al (2002) that
reported banteng bull semen had watery milk in
colour [2]. Semen consistency obtained from three
bulls in this study was vary from watery to
moderatly thick.
Volume of semen samples that were
succesfully collected in this study ranged from 4-
7,4 ml with mean+SE value of 6,3+0,78 ml. This
result was lower compared to the volume of semen
collected by electroejaculation from closely related
species, Bali bull, which had mean+SE value of
6,98+0,29 ml (4). The pH value of the semen in this
study were 6,4-6,8 in range, while the mean+SE
value for this parameter was 6,625+0,10.This
result was comparable with previous study that
reported pH value of 6,6 [2].
Velocity scores obtained in this study were
close in agreement with earlier report although
one sample showed lower score [2]. The mean+SE
value of percentage of viability of banteng bull
sperm were lower compared to that of Bali bulls
[4]. Some samples were lower in percentage of
viability compared to tprevious report [2].
Figure 1 Semen collection of banteng bull using
electroejaculator. (a) Rectal probe was inserted
through the anus of anasthetized male banteng, (b)
Semen sample was recovered inside collection
tube.
The lowest sperm concentration in this
study comparable with previous finding [2].
However, the mean+SE value of sperm
concentration in this study which was was still
above the sperm concentration of semen from Bali
bulls [4]: Iswadi et al (2007) had reported sperm
concentration of 400 million to 10200 million
sperm ml from gaur semen collected by
3. Proc. of the 20th FAVA CONGRESS & The 15thKIVNAS PDHI, Bali Nov 1-3, 2018 | 63
electroejaculation. That range was considered as
very good sample [3].
Percentage of progressive motility and
viability were vary among samples and relatively
lower than semen volume reported by previous
study which were 70% and 83%, respectively [2].
Percentage of progressive motility was also vary
among samples. This finding also reported by
Iswadi et al. (2007) that found the percentage of
progressive motility that vary from 16% to 70% in
range from gaur semen collected by
electroejaculation [3].
Morphologically normal sperm is
neccessary for fertilisation. In this study,
percentage of morphologically normal sperm
ranged from 67,22 to 86,75, with mean+SE value of
74,54+5,58. The highest value of sperm
concentration of this study was higher than
previous study which was only 77% [2], The mean
value+SE was also higher compared to those in Bali
bulls [4].
CONCLUSION
The present study has successfully
provided data of semen characteristics of banteng
collected by electroejaculation method with larger
number of samples. The result will be useful for
assisted reproductive technology in this species
and for further research on male reproducitve
biology.
ACKNOWLEDGMENTS
The authors would like to thank Taman
Safari Bogor, Indonesia and Ragunan Zoo, Jakarta,
Indonesia for the permission to publish the data.
This study is supported by USAID through
Sustainable Higher Education Research Alliances
(SHERA) Program-Center for Collaborative
Research Animal Biotechnology and Coral Reef
Fisheries (CCR ANBIOCORE) and Directorate
General of Higher Education (DGHE) Indonesia
through the PMDSU Study Grant Program.
REFERENCES
[1] Gardner PC, Pudyatmoko S, Bhumpakphan N,
Yindee M, Ambu DLN, Goossens B. 2016.
Banteng Bos javanicus d’Alton, 1823 Ecology,
Evolution and Behaviour of Wild Cattle:
Implications for Conservation, ed. M. Melletti
and J. Burton. Cambridge University Press,
Cambridge, p.217
[2] Johnston SD, McGowan MR, Blyde D. 2002.
Birth of a Banteng (Bos javanicus)
calf at Western Plains Zoo after fixed time
artificial insemination. Aust Vet J Vol 80, Nos 1
& 2, January, February 2002
[3] Iswadi MI, Hafiz MM, Hafiz MD, Fahrul FJ,
Hajarian H, Wahid H, Zawawi I, Khairiah MS,
Mazni OA. 2012. Collection, analysis and
cryopreservation of semen from Malayan gaur
(Bos gaurus hubbacki): A preliminary study.
Open Veterinary Journal, (2012), Vol. 2: 109-
114
[4] Sarsaifi K, Rosnina Y, Ariff MO, Wahid H, Hani
H, Yimer N, Vejayan N, Naing SW, Abas MO.
2013. Effect of Semen Collection Methods on
the Quality of Pre- and Post-thawed Bali Cattle
(Bos javanicus) Spermatozoa. Reprod Dom
Anim 48, 1006–1012