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Primer design

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1. PCR primer design • Local software Primer premier5, Oligo 6.1 • Web-based design Primer3 (Whitehead Institute/MIT Center for Genome Research)
Primer premier5
Principles for primer design • Base composition • Length • Repeated and self-complementary seq. • Cross dimer • Tm • 3’ Termini • Priming sites (From Molecular cloning 3, 8.14)

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Primer design

  • 1. 1. PCR primer design • Local software Primer premier5, Oligo 6.1 • Web-based design Primer3 (Whitehead Institute/MIT Center for Genome Research)
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11. Principles for primer design • Base composition • Length • Repeated and self-complementary seq. • Cross dimer • Tm • 3’ Termini • Priming sites (From Molecular cloning 3, 8.14)