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Primer Design
1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)
Gene Runner 1.Sequence Insertion
 
 
 
 
 
 
Oligo 6
 
 
 
 
 
 
Manual 1. Reference  sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. B...
HCV Genome
Alignment
 
Current oligo length Position number
Non specific band
 
 
 
 
- Length <ul><li>Miss match </li></ul><ul><li>*5-7nt from 3’ end </li></ul>
 
 
 
 
 
 
 
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Primer Designing

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Primer Designing

  1. 1. Primer Design
  2. 2. 1. Gene Runner 2. Oligo 6 4. Manual 3. Primer 3(on line)
  3. 3. Gene Runner 1.Sequence Insertion
  4. 10. Oligo 6
  5. 17. Manual 1. Reference sequence & other sequences (NCBI) 2. Alignment (Mega 4) 3. Sequence selection (In selected gene) 4. Blast (NCBI) 5. TM ( oligo analyzer , oligo6, gene runner) 6. Secondary structures (loop, hairpin,…) 7. False priming (oligo 6)
  6. 18. HCV Genome
  7. 19. Alignment
  8. 21. Current oligo length Position number
  9. 22. Non specific band
  10. 27. - Length <ul><li>Miss match </li></ul><ul><li>*5-7nt from 3’ end </li></ul>

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