This study compared the efficacy of PQ Multi-Enzyme Cleaner and ANIOSYME cleaner. Tests found that PQ Multi-Enzyme Cleaner removed all protein soil after 30 minutes at concentrations of 0.5-1.0%, while ANIOSYME left considerable residue. PQ Multi-Enzyme Cleaner also caused no corrosion to stainless steel, aluminum, copper or brass, whereas ANIOSYME caused changes in appearance and weight loss in aluminum, copper and brass. In conclusion, PQ Multi-Enzyme Cleaner demonstrated greater detergency and anti-corrosion effects compared to ANIOSYME.

1. September 22th, 2014
STUDY DIRECTOR
Biochemical Laboratory
Emiko Kawamukai
RESEARCH STAFF
Biochemical Laboratory
Yuka Oda
STUDY REPORT
Efficacy Comparison between PQ Multi-Enzyme Cleaner
for Manual Soaking (Weak Alkaline, Low-foaming)
and ANIOSYME DD1
Table of Contents
1. Introduction···························································1
2. Materials and methods ············································1
3. Results ································································2
4. Summary······························································5
BCL No. 26742

2. 1
1．Introduction
This report includes the results of efficacy comparison tests conducted on Power
Quick Multi-Enzyme Cleaner for Manual Soaking (Weak alkaline, Low-foaming; hereafter
“PQ Multi-Enzyme Cleaner”) and ANIOSYME DD1 (hereafter “ANIOSYME”).
2．Materials and methods
2-1. Cleaners used in the tests
The cleaners used in the tests are shown in Table 1. ANIOSYME (provided by
Global Operations Division of SARAYA in May 2013) contains quaternary ammonium salts
and PHMB and is used for cleaning and pre-disinfection, according to the description on its
label.
Table 1. Cleaners used in the tests
PQ Multi-Enzyme Cleaner ANIOSYME
Manufacturer SARAYA ANIOS
Appearance of
undiluted solution
Clear blue Clear blue
Odor Raw material odor Raw material odor
Application*1 Immersion cleaning, manual
cleaning, ultrasonic cleaning
Cleaning and pre-disinfection
(immersion and ultrasonic)
Use concentration*1
Recommended use
concentration: 1.0%
(0.5 to 2.0% according to the
severity of soil)
0.5%
Components*1
Surfactant (sophorolipid),
alkaline agent, protease,
lipase, amylase, cellulase,
enzyme stabilizer, corrosion
inhibitor
Quaternary ammonium salts,
PHMB, protease, lipase,
amylase, surfactant, stabilizer,
chelating agent, fragrance,
coloring agent
pH
Undiluted 8.2 6.0
Use solution 9.2（1.0%） 6.7（0.5%）
*1
Quoted from the label and the product information of each product
2-2. Detergency
Cleaning efficacy indicator TOSI (PEREG, Waldkraiburg, Germany) was used in
the tests. The concentrations of the cleaners used in the tests were 0.5% and 1.0%, which
were their recommended use concentrations. TOSIs were immersed in the use solutions of
the test cleaners for 10 or 30 minutes at 40℃. After immersion, TOSIs were taken out and

3. 2
rinsed lightly in running water, and then were dried and stained using Power Quick
Residual Protein Detector (SARAYA).
2-3. Anti-corrosion effect
Test metal pieces (stainless steel (SUS304, SUS430, SUS420J2), aluminum,
copper and brass in a size of 50mm × 30mm × 0.8mm) were immersed in use solutions of
PQ Multi-Enzyme Cleaner and ANIOSYME, and were set aside for seven days at 50℃.
After immersion, the test pieces were rinsed in water and dried, and the changes in their
appearance and weight were examined.
3．Results
3-1. Detergency
Test results are shown in Table 2. PQ Multi-Enzyme Cleaner removed the soil
totally after 30-minute immersion at the concentrations of both 0.5% and 1.0%. In contrast,
ANIOSYME resulted in considerable soil residue after 30-minute immersion regardless of
the concentration. Quaternary ammonium salts and PHMB, which are the cleaning and
disinfecting ingredients of ANIOSYME, are known to cause adhesion of protein soil1
. This
information was confirmed by the test results.
Table 2. Results of detergency tests
Cleaner 10 min 30 min Cleaner 10 min 30 min
PQ
Multi-Enzyme
Cleaner
0.5%
ANIOSYME
0.5%
1.0% 1.0%
1
Fushimi R, Hanamura R, Nakata S, et al.: Problems of Cleaning caused by
Contaminated Objects after Disinfection. The Japanese Journal of Medical Instrumentation,
73(6): 281-289, 2003.

4. 3
3-2. Anti-corrosion effect
Results are shown in Tables 3 and 4. PQ Multi-Enzyme Cleaner caused no
changes on all test metal pieces regardless of the concentration. In contrast, ANIOSYME
caused changes in appearance and weight on aluminum, copper and brass test pieces.
Table 3. Results of anti-corrosion test
Test piece
Change in appearance Change in weight (mg)
PQ Multi-Enzyme
Cleaner(0.5%)
Stainless steel
(3 grades)
No Change 0
Aluminum No Change 0
Copper No Change 0
Brass No Change 0
PQ Multi-Enzyme
Cleaner (1.0%)
Stainless steel
(3 grades)
No Change 0
Aluminum No Change 0
Copper No Change 0
Brass No Change 0
ANIOSYME
(0.5%)
Stainless steel
(3 grades)
No Change 0
Aluminum No Change -1.3
Copper Turned brown -0.2
Brass Turned brown -1.6
ANIOSYME
(1.0%)
Stainless steel
(3 grades)
No Change 0
Aluminum Turned slightly white in color -1.3
Copper Turned brown -0.4
Brass Turned brown -1.9
Tap water
Stainless steel
(3 grades)
No Change 0
Aluminum Turned black 0
Copper Turned dull 0
Brass Brown spots observed 0

5. 4
Table 4. Metal test pieces after anti-corrosion test
SUS304 SUS420J2 SUS430 Aluminum Copper Brass
PQ Multi-
Enzyme
Cleaner
(0.5%)
PQ Multi-
Enzyme
Cleaner
(1.0%)
ANIOSYME
(0.5%)
ANIOSYME
(1.0%)
Tap water
Before the
test

6. 5
4．Summary
Comparison between PQ Multi-Enzyme Cleaner and ANIOSYME is summarized
in Table 5. PQ Multi-Enzyme Cleaner was confirmed to have greater efficacy than
ANIOSYME in both detergency and anti-corrosion effect.
Table 5. Comparison between PQ Multi-Enzyme Cleaner and ANIOSYME (Summary)
Cleaner
PQ Multi-Enzyme
Cleaner
ANIOSYME
Manufacturer SARAYA ANIOS
Appearance of undiluted
solution
Clear blue Clear blue
Odor Raw material odor Raw material odor
pH
Undiluted 8.2 6.0
Use solution 9.2（1.0%） 6.7（0.5%）
Concentration of use solution
used in the tests
0.5% 1.0% 0.5% 1.0%
Detergency*1
○ ○ × ×
Anti-corrosion*2
SUS (3 grades)
Aluminum
Copper
Brass
○
○
○
○
○
○
○
○
○
×
×
×
○
×
×
×
*1
30-minute immersion: ○ totally removed the soil; △ left small amount of soil to be removed;
× left large amount of soil to be removed
*2
○ Change in neither appearance nor weight; × Change in appearance and / or weight
(End of the document)