This study examined the growth of Spirulina algae when exposed to copper and acetaldehyde in two culturing methods: airlift loop bioreactors (ALBs) and flasks. Spirulina was able to acclimate to increasing copper concentrations in the ALBs but not flasks, possibly due to higher toxicity in flasks. Growth rates were higher in ALBs due to better gas exchange. Results from testing different concentrations of copper and acetaldehyde on Spirulina growth were analyzed to determine acclimation ranges.