Pharmacology laboratory experiment, both invivo and invitro includes interpolation, matching , bracketing, three point, four point bioassays with a note on hypoglycemic activity, acute skin irritation, acute eye irritaiton, pyrogen test, gastrointestinal motility test, physiological salt solutions

1. Pharmacology
Laboratory Experiments
Koppala R V S Chaitanya
Dept. of Pharmacology

2. S.NO TITLE Page No
1 Introduction to invitro pharmacology and physiological salt solutions (PSS) 01
2 Various Techniques For Isolated Tissues Or Organ Related Experiments 24
3 Bioassays, its types including advantages and disadvantages 30
4 Concentration Response Curve Of Acetylcholine Using Isolated Rectus Abdominus Muscle
Preparation Of Frog
48
5 Effect of Physostigmine on DRC of Acetylcholine Using Frog Rectus Abdominus Muscle 72
6 Effect of Atropine on DRC of Acetylcholine Using Rat Ileum 91
7 Bioassay of Oxytocin Using Rat Uterine Horn by Interpolation Method 110
8 Bioassay of Histamine Using Guinea Pig Ileum by Matching Method 133
9 Bioassay of Serotonin Using Rat Fundus Strip by Three Point Bioassay 148
10 Bioassay of Acetylcholine Using Rat Ileum by Four Point Bioassay 168
11 Bioassay of Acetylcholine Using Rat Ileum by Four Point Bioassay 186
12 Anti Ulcer Activity Of A Drug Using Pylorus Ligand (SHAY) Rat Model 204
13 Anti-ulcer Activity of a Drug Using NSAIDS Induced Ulcer Model 214
14 Effect of Saline Purgative on Frog Intestine 226
15 Effect of Spasmogens and Spasmolytics Using Rabbit Jejunum 236
16 Effects of Drugs on Gastrointestinal Motility 251
17 Hypoglycaemic Effect of Insulin In Rabbit 270
18 Study of Mydriatic And Miotic Effects on Rabbit Eye 278
19 Test for Pyrogens (Rabbit method) 298
20 Determination of Acute Eye Irritation/Corrosion of A Test Substance 315
21 Determination of Acute Skin Irritation/Corrosion of A Test Substance 331
22 Calculation of pharmacokinetics parameters from given data 351

3. Introduction to invitro
pharmacology and physiological
salt solutions (PSS)
1

4. 2

5. 3

6. 4

7. 5

8. 6

9. 7

10. 8

11. 9

12. 10

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14. 12

15. 13

16. 14

17. 15

18. 16

19. 17

20. 18

21. 19

22. 20

23. 21

24. 22

25. 23

26. Various Techniques For Isolated
Tissues Or Organ Related
Experiments
24

27. 25

28. 26

29. 27

30. 28

31. 29

32. Bioassays, its types
including advantages and
disadvantages
30

33. 31

34. 32

35. 33

36. 34

37. 35

38. 36

39. 37

40. 38

41. 39

42. 40

43. 41

44. 42

45. 43

46. 44

47. 45

48. 46

49. 47

50. Concentration Response Curve Of
Acetylcholine Using Isolated
Rectus Abdominus Muscle
Preparation Of Frog
48

51. 49

52. 50

53. 51

54. 52

55. 53

56. 54

57. 55

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70. 68

71. 69

72. 70

73. 71

74. Effect of Physostigmine on DRC
of Acetylcholine Using Frog
Rectus Abdominus Muscle
72

75. 73

76. 74

77. 75

78. 76

79. 77

80. 78

81. 79

82. 80

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90. 88

91. 89

92. 90

93. Effect of Atropine on DRC of
Acetylcholine Using Rat Ileum
91

94. 92

95. 93

96. 94

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99. 97

100. 98

101. 99

102. 100

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104. 102

105. 103

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109. 107

110. 108

111. 109

112. Bioassay of Oxytocin Using Rat
Uterine Horn by Interpolation
Method
110

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131. 129

132. 130

133. 131

134. 132

135. Bioassay of Histamine Using
Guinea Pig Ileum by Matching
Method
133

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138. 136

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140. 138

141. 139

142. 140

143. 141

144. 142

145. 143

146. 144

147. 145

148. 146

149. 147

150. Bioassay of Serotonin Using Rat
Fundus Strip by Three Point
Bioassay
148

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168. 166

169. 167

170. Bioassay of Acetylcholine Using
Rat Ileum by Four Point
Bioassay
168

171. 169

172. 170

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177. 175

178. 176

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180. 178

181. 179

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186. 184

187. 185

188. Bioassay of Acetylcholine Using
Rat Ileum by Four Point
Bioassay
186

189. 187

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194. 192

195. 193

196. 194

197. 195

198. 196

199. 197

200. 198

201. 199

202. 200

203. 201

204. 202

205. 203

206. Anti Ulcer Activity Of A Drug
Using Pylorus Ligand (SHAY) Rat
Model
204

207. 205

208. 206

209. 207

210. 208

211. 209

212. 210

213. 211

214. 212

215. 213

216. Anti-ulcer Activity of a Drug
Using NSAIDS Induced Ulcer
Model
214

217. 215

218. 216

219. 217

220. 218

221. 219

222. 220

223. 221

224. 222

225. 223

226. 224

227. 225

228. Effect of Saline Purgative on
Frog Intestine
226

229. 227

230. 228

231. 229

232. 230

233. 231

234. 232

235. 233

236. 234

237. 235

238. Effect of Spasmogens and
Spasmolytics Using Rabbit
Jejunum
236

239. 237

240. 238

241. 239

242. 240

243. 241

244. 242

245. 243

246. 244

247. 245

248. 246

249. 247

250. 248

251. 249

252. 250

253. Effects of Drugs on
Gastrointestinal Motility
251

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262. 260

263. 261

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270. 268

271. 269

272. Hypoglycemic Effect of
Insulin In Rabbit
270

273. 271

274. 272

275. 273

276. 274

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278. 276

279. 277

280. Study of Mydriatic And Miotic
Effects on Rabbit Eye
278

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300. Test for Pyrogens
(Rabbit method)
298

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311. 309

312. 310

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314. 312

315. 313

316. 314

317. Determination of Acute Skin
Irritation/Corrosion of A Test
Substance
315

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322. 320

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324. 322

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327. 325

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330. 328

331. 329

332. 330

333. Determination of Acute Eye
Irritation/Corrosion of A Test
Substance
331

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345. 343

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351. 349

352. 350

353. Calculation of pharmacokinetics
parameters from given data
351

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