Library Construction and Screening Fig.1 Workflow of phage display construction and screening (Díez et al. 2015) Phage display is one of the most powerful and widely used laboratory technique for the study of protein-protein, protein-peptide and protein-DNA interactions. This technology is mainly based on displaying the interest protein (peptides, antibodies, scaffolds or others) on the surface of employing phage and then be used to interrogate the constructed libraries containing millions or even billions of displayed phages. Theoretically, phage display is an exogenous gene expression method which the gene encoding the interest protein is inserted into bacteriophage coat protein gene then displaying the interest protein on the phage surfaces, resulting in a connection between genotype and phenotype.

1. phage panning
• Creative Biolabs is one of the well-recognized experts who is
professional in applying advanced phage display technologies for a
broad range of project objectives. With years of experience, our
scientists can offer high-quality phage display library construction and
custom phage display library screening services to meet our clients’
demands precisely. Particularly, our services also involve specific
antibody discovery (e.g. PTM-specific antibody, anti-idiotype antibody,
and agonistic antibody) and peptidome discovery.

2. • Phage display is one of the most powerful and widely used laboratory
technique for the study of protein-protein, protein-peptide and
protein-DNA interactions. This technology is mainly based on
displaying the interest protein (peptides, antibodies, scaffolds or
others) on the surface of employing phage and then be used to
interrogate the constructed libraries containing millions or even
billions of displayed phages. Theoretically, phage display is an
exogenous gene expression method which the gene encoding the
interest protein is inserted into bacteriophage coat protein gene then
displaying the interest protein on the phage surfaces, resulting in a
connection between genotype and phenotype.

3. • Due to the different properties and advantages, Creative Biolabs is
pleased to tailor the most appropriate phage display system (M13, T4
or T7) to meet our customers’ demands. Through DNA
manipulation, numerous gene variants can be created and
constructed as phage display library. Our scientists are confident in
generating high-quality libraries with the diversity of 108.
Furthermore, to detect the interaction between displayed protein and
those other molecules or isolate specific target binders, Creative
Biolabs also utilizes the novel biopanning strategies to select high-
affinity binders or ligands which are able to recognize the naïve
targets.

4. • Antibody libraries are constructed by the genomic information coding
for antibody variable domains, which can be derived from B cells of
immune or naïve donors. Antibodies are the first proteins which were
successfully displayed on the surface of phage by fusing the coding
sequence of scFv or Fab to the coat protein. Due to the smaller size,
scFv libraries are genetically more stable than Fab libraries, while Fab
libraries lack the tendency to form higher molecular weight species,
such as dimers and trimers, which can simplify the selection and
characterization. Through binding the specific target, the interest
antibody can then be eluted and retrieved.

5. • Compared with the traditional hybridoma method, antibody phage
display library has distinct advantages on discovering novel
monoclonal antibodies and even the fully human antibody. Creative
Biolabs is able to construct immune antibody libraries and isolate
monoclonal antibodies with high specificity and affinity from a
comprehensive list of species, which including but not limited to
monkey, llama, camel, shark, alligator, mouse, rat, hamster, guinea pig,
rabbit, chicken, dog, bovine, goat, sheep, and ferret. In addition, our
scientists also possess some high-quality pre-made libraries (including
human antibody libraries) for our library screening services.