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Qualitative Determination of Pesticides in a Roma Tomato, Iceberg Lettuce, and a Macintosh Apple Karen Sanders Chemistry 481 12/4/08 1
Varian Saturn 3800-2200 GC/MS ,[object Object],2
Restek Pesticide Standard ,[object Object],[object Object],[object Object]
 
Varian Saturn 3800-2000 GC-MS Parameters for Restek Standard and Roma Tomato Separation Split-less Mode: 30 m x 0.25 mm ID, 0.25 um Column Size: 5 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 5% Phenyl-95% polydimethylsiloxane Stationary Phase: 1.8 ml/min Flow Rate:  1 mL Volume Injected: Helium Carrier Gas:
Restek Standard of Organochlorine Pesticide Mix AB#2: Chromatogram 18/20 Pesticides Found
Roma Tomato Kroger Brand  Originated from  California, U.S.A. Unwashed prior to  analysis
Reagents ,[object Object],[object Object],[object Object]
Pesticide Extraction Procedure for the Roma Tomato ,[object Object],[object Object],[object Object],[object Object],[object Object]
Pentadecanoic acid, 13-methyl ester  Dibutyl Pthalate Tomato Extract: Total Ion Current Chromatogram
Ion Search of Mass Spectrum ,[object Object],[object Object],[object Object],[object Object]
Iceberg Lettuce Ocean Mist Brand Originated from the  Ocean Mist Farms in  California, U.S.A Unwashed prior to  analysis
Pesticide Extraction Procedure for the Iceberg Lettuce ,[object Object],[object Object]
Varian Saturn 2000 GC-MS Parameters for Iceberg Lettuce Separation Split Mode: 50 minutes Total Time of Run 10 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 2 mL Injection Volume 1.8 ml/min Flow Rate:  Helium Carrier Gas:
Iceberg Lettuce Extract: Total Ion Current Chromatogram
Ion Search of Mass Spectrum ,[object Object],[object Object],[object Object],[object Object],[object Object]
Macintosh Apple Apple Ridge Brand  Macintosh Apple Originated from Jack  Brown Produce in  Sparta, MI  U.S.A. Unwashed before  analysis
Pesticide Extraction Procedure for the Macintosh Apple ,[object Object],[object Object],[object Object],[object Object]
Varian Saturn 2000 GC-MS Parameters for Macintosh Apple Separation Split Mode: 30 minutes Total Time of Run 10 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 3 mL Injected Volume: 1.8 ml/min Flow Rate:  Helium Carrier Gas:
Macintosh Apple Extract: Total Ion Current Chromatogram
Ion Search of Mass Spectrum ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Sources of Error  ,[object Object],[object Object]
References ,[object Object],[object Object],[object Object],[object Object]

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Pesticide Presentation

  • 1. Qualitative Determination of Pesticides in a Roma Tomato, Iceberg Lettuce, and a Macintosh Apple Karen Sanders Chemistry 481 12/4/08 1
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  • 5. Varian Saturn 3800-2000 GC-MS Parameters for Restek Standard and Roma Tomato Separation Split-less Mode: 30 m x 0.25 mm ID, 0.25 um Column Size: 5 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 5% Phenyl-95% polydimethylsiloxane Stationary Phase: 1.8 ml/min Flow Rate: 1 mL Volume Injected: Helium Carrier Gas:
  • 6. Restek Standard of Organochlorine Pesticide Mix AB#2: Chromatogram 18/20 Pesticides Found
  • 7. Roma Tomato Kroger Brand Originated from California, U.S.A. Unwashed prior to analysis
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  • 10. Pentadecanoic acid, 13-methyl ester Dibutyl Pthalate Tomato Extract: Total Ion Current Chromatogram
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  • 12. Iceberg Lettuce Ocean Mist Brand Originated from the Ocean Mist Farms in California, U.S.A Unwashed prior to analysis
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  • 14. Varian Saturn 2000 GC-MS Parameters for Iceberg Lettuce Separation Split Mode: 50 minutes Total Time of Run 10 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 2 mL Injection Volume 1.8 ml/min Flow Rate: Helium Carrier Gas:
  • 15. Iceberg Lettuce Extract: Total Ion Current Chromatogram
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  • 17. Macintosh Apple Apple Ridge Brand Macintosh Apple Originated from Jack Brown Produce in Sparta, MI U.S.A. Unwashed before analysis
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  • 19. Varian Saturn 2000 GC-MS Parameters for Macintosh Apple Separation Split Mode: 30 minutes Total Time of Run 10 minutes Solvent Delay: 270 °C Injector Port Temp: 275°C Oven Temp: increasing to 265 °C at 10 °C/min, and holding for 10 min. 80 °C for 1 minute, increasing to 240 °C at 10 °C/min, Column Temp: 3 mL Injected Volume: 1.8 ml/min Flow Rate: Helium Carrier Gas:
  • 20. Macintosh Apple Extract: Total Ion Current Chromatogram
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