Performance Review Form 1 (Dec 2022) Adina Georgiana Dorobantu.pdf

1. APPENDIX 3: Performance Review Documents
STUDENT: Adina Georgiana
Dorobantu
PERIOD
SHEFFIELD HALLAM
UNIVERSITY
ACTIVITY REVIEW 1. 22/12/2022
SUPERVISOR: Ian Coldicott 2.
TO BE COMPLETED BY THE STUDENT 3.
ACTIVITIES MAIN SKILLS ACQUIRED/USED SKILL AREAS
(Technical / Personal/Operational) New
?
A - H

2. General Technical Team duties
Organised and completed an audit of all
products/ items found in stock with the purpose
of re-organising the stock layout for better
accessibility, efficiency and safety and starting a
weekly stock count activity that would allow a
higher level of control and understanding of
space and stock usage and demand from a
weekly, monthly and yearly perspective.
Using the autoclave and preparing Eppendorf
tube bags, water and PBS to be autoclaved; re-
stocking and re-filling of consumables; delivery
reception.
Waste disposal.
Liquid nitrogen top up for dewars used for
sample storage – appropriate health and safety
measures.
Stock ordering and organisation system.
Attended and participated in the technical team
meetings.
Organisational, planning and time management
skills.
Strongly developed leadership, decision
making and critical and objective analysis skills,
as well as showing good initiative.
Good team working ability shown from the start
of the project until the end, including being part
of team meetings, having initiative, being able
to spot, raise and discuss potential difficulties
or bottlenecks that might affect the project,
active suggestions for solutions and
improvement, regular re-evaluations of the
progress made and deciding on a plan to go
forward.
Created an Excel spreadsheet containing
detailed information about stock items to be
used in weekly stock counts and to be passed
on as a helping tool for next year students.
A, B, C, D, E, H (i), (iii)
A, B, C, D, E, G, H (i), (ii), (iii)

3. Tissue Culture
Mycoplasma testing
Planning to keep weekly and monthly records
and reports of stock count in order to generate
a yearly report of stock usage.
Developed adaptability skills.
Maintaining fibroblast cell growth with regular
DMEM feeds; splitting the cells when the
fibroblast confluence is over 90%; making up
DMEM media, aliquoting vitamins, pen/strep
and FBS; making up uridine; correct use of
PPE; good time management, planning and
record keeping skills.
General tissue culture lab duties: correct use,
cleaning and maintenance of the centrifuge,
biological safety cabinets and CO2 incubator;
making up Virkon and 70% IMS; re-stock of
consumables.
Performing a luminescence assay on fibroblast
cell cultures using the MycoAlert Kit.
Use of Pherastar equipment and software.
Generating the results using Excel.
Interpreting, communicating and discussing the
results and potentially re-testing, with the
appropriate people.
Making up the substrate, reagent, negative and
positive controls from the MycoAlert Kit.
B, C, D, E, H (i), (ii), (iii)
B, D, E, F, G, H (i), (ii), (iii)

4. Histology activities
Changing the absolute alcohol, xylene, Harris
haematoxylin and eosin staining pots
Microtome sectioning
Use and disposal of absolute ethanol, xylene.
Filtering eosin and Harris haematoxylin
Appropriate waste disposal.
Appropriate use of fume hood while handling
xylene.
Staining pots – dishwasher cleaning.
Use of the dishwasher.
Preparation of wax blocks to be cut by leaving
them on ice for at least 1 h before starting for
best results.
Correct use of the microtome: adjusting the
position of the block, changing the blade,
appropriate cleaning of all parts and removal of
excess wax using xylene, correct use of
appropriate safety measures.
Using the water bath.
Using the correct technique for picking up
tissue sections from the water bath using
different types of glass slides.
Organisation, time management and record
keeping skills.
Waste disposal (sharps, anatomical waste).
H (i), (ii)
D, E, H (i), (ii), (iii)

5. Staining
Mice genotyping
Performed the following stains:
● H&E
● Toluidine Blue
● Perl’s Prussian Blue
● Masson`s Trichrome
● Periodic Acid Schiff (PAS)
● Cresyl Fast Violet
Planning, time management, record keeping,
organisational skills.
Cover slipping – using DPX.
Making up different solutions required for
different stains.
Interpretation of the stained slides under the
microscope.
DNA extraction from mouse ear clips using
DNA QuickExtract.
Preparation of the PCR mastermix.
Correct and clear tube labelling.
PCR program setup.
Storage of PCR samples in the freezer until
further processing.
Performing an agarose gel electrophoresis
using the PCR products as part of the
genotyping process.
Preparation of agarose gel.
D, E, G, H (i), (ii)
C, D, E, F, G, H (i), (ii), (iii)

6. Retrieving and returning anatomical samples
from the Sheffield Brain Tissue Bank
Correct handling of ethidium bromide - health
and safety measures and PPE.
Use of the Gbox for gel imaging.
Following the health and safety regulations
when using the Gbox.
Waste disposal streams.
Results interpretation and troubleshooting.
Good decision making and time management
skills.
Planning skills.
A general understanding of the brain donation,
processing, storing conditions and associated
procedures and regulations.
A, B, C, D, E, H (i), (ii), (iii)

7. Sheffield Hallam University: Performance
Review
Student: Adina Georgiana Dorobantu
Supervisor: Ian Coldicott
First: 22/12/2022
Mid:
Final:
SKILL AREA Student
Examples / Comments
Supervisor
Examples / Comments
A. Professional & Organisational
Awareness
● General presentation
● Equality & diversity
● Professional ethics
● Confidentiality
● Company structure & function
● Departmental structure & function
● Commercial awareness
General Technical Team duties
Attending and participating in the technical team
meetings
Stock room reorganisation
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
8/10
Adina is aware of her role within the
structure of the department and the
function of the university as a whole. Her work in
reorganising the department’s lab consumables
stock demonstrates a good commercial
awareness.
Score
8/10
B. Communication
● Clear verbal communication &
active listening
● Presentations
● Explanatory ability
● Writing skills
● Communicating with people from
different levels / backgrounds
General Technical Team duties
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mycoplasma Testing
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
8/10
Adina communicates clearly with colleagues at all
levels, providing results from laboratory tests she
performs and participating in team meetings.
Score
8/10
C. Working with Others General Technical Team duties

8. ● Cooperating
● Contributing to teamwork
● Working under supervision
● Supervising / showing leadership
● Working with different people
● Interdisciplinary working
● Emotional intelligence
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
7/10
Adina has quickly become a valued member of
the technical team, working under supervision as
needed, as well as demonstrating lab techniques
to others.
Score
8/10
D. Self-Improvement
● Enthusiasm, commitment
● Willingness to learn
● Initiative, resourcefulness,
autonomy
● Taking responsibility
● Maturity
● Effective time management
● Personal organisation / work
planning
General Technical Team duties
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mycoplasma testing
Microtome sectioning
Staining
Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
8/10
Adina has consistently demonstrated a keenness
to improve her knowledge and learn new skills,
frequently performing independent research to
enhance her understanding of laboratory
procedures. She has taken responsibility for
performing weekly mycoplasma testing for our
department’s tissue culture users and always
plans her time well.
Score
9/10
E. Problem Solving & Resilience General Technical Team duties

9. ● Prioritising tasks
● Defining problems
● Taking action
● Locating information
● Taking a range of approaches
● Persevering
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mycoplasma testing
Microtome sectioning
Staining
Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
8/10
Adina is well-organised and prioritises her
workload appropriately. Where problems have
arisen with her lab work she has sought advice
and researched potential solutions, and
persevered with techniques which are difficult to
master
Score
8/10
F. Information technology
● Using Word/Excel/PP etc.
● Using specialist software
● Using databases
● Literature searching
● Open to developing and utilising
new technology skills
Mycoplasma testing
Mice genotyping
Score
6/10
Adina demonstrates a good working knowledge of
IT, using Microsoft/Google products proficiently
and performing literature searches as needed to
support her learning.
Score
7/10

10. G. Numeracy
● Calculating / evaluating
● Checking results
● Applying appropriate statistics
● Recording / presenting data
Stock room reorganisation
Mycoplasma testing
Staining
Mice genotyping
Score
6/10
Adina demonstrates good numeracy skills in
calculating and presenting results of experiments.
Score
7/10
H. Working Practice
(i) Knowledge
● Experimental/project design
● Function / principles of equipment
● Health & safety procedures
● Knowing reasons for particular
working practices
● Background / theoretical
knowledge
General Technical Team duties
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mycoplasma testing
Changing the absolute alcohol, xylene, Harris
haematoxylin and eosin staining pots
Microtome sectioning
Staining
Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
7/10
Adina has learned the function and principles of
operation of various items of lab equipment, and
performs independent reading to further her
understanding. She understands and follows our
health & safety procedures.
Score
7/10

11. (ii) Work Skills
● Careful & safe working practice
● Manual dexterity
● Correct use of
equipment/methodology
● Follows methods / protocols
● Competent in a range of activities
General Technical Team duties
Stock room reorganisation
Tissue Culture
Mycoplasma testing
Changing the absolute alcohol, xylene, Harris
haematoxylin and eosin staining pots
Microtome sectioning
Staining
Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
8/10
Adina carefully follows protocols for various lab
procedures and uses work equipment correctly.
Score
8/10
(iii) Quality of Work
● Accuracy
● Reliability of service
● Attention to details
● Efficiency
● Meets deadlines
● Resilience under pressure
General Technical Team duties
Attending and participating in the technical team
meetings
Stock room reorganisation
Tissue Culture
Mycoplasma testing
Microtome sectioning
Adina works efficiently and accurately, with good
attention to detail in a range of duties.

12. Mice genotyping
Retrieving and returning anatomical samples from
the Sheffield Brain Tissue Bank
Score
7/10 Score
7/10
TO BE COMPLETED BY THE SUPERVISOR
Comments / Proposals
Potential action to assist with training, extend
experience or address problems as follow-up
to Performance Review
Adina has become a valuable member of our team in the short time that she has been with us,
demonstrating an excellent work ethic and eagerness to learn. We will aim to expand her repertoire of
laboratory skills over the coming months, particularly in histology and molecular biological techniques.
In terms of her employability and professional behaviours as recorded on this form, Adina already
scores very highly so has very little room for improvement! If future scores are no higher than in this
first review, this should in no way be interpreted as a lack of progress.
Date discussed: 03.01.2023 Supervisor: Student:

13. Score Level of Achievement
10 Excellent (exceptional first)
9 Excellent (exceptional first)
8 Excellent (exceptional first)
7 above average (first)
6 good, average (upper second)
5 satisfactory, clear pass (lower second)
4 bare pass (third)
3 poor, borderline fail
2
1
↓
0 completely unsatisfactory