This study characterized fine particulate matter (PM2.5 and PM1) concentrations and chemical composition from samples collected at a roadside location in Athens, Greece. Samples were taken at two heights (1st and 5th floor) along the open road and within a street canyon. Average daily PM concentrations did not exceed recommended limits but were higher along the open road. PM1 accounted for over 90% of PM2.5 mass at the 5th floor location, indicating an increase in ultrafine particles with height. Vertical profiles showed significant differences in PM1/PM2.5 ratios between floors within the street canyon. The study aims to evaluate the carcinogenic and mutagenic potential of traffic-related PM at different sampling heights