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EURL CPS, Lm, MMP
                                                                                                                                   European Union Reference Laboratory for
                                                                                                                                   Coagulase Positive Staphylococci
                                                                                                                                   Listeria monocytogenes
                                                                                                                                   Milk and Milk Products
Maisons-Alfort Laboratory for
                 Food Safety




            Overview of the European Union Reference
           Laboratory (EURL) for Listeria monocytogenes
                   activities in Anses Laboratory

                                                      Adrien ASSERE
                            Deputy manager of EU reference Laboratories for
                  Listeria monocytogenes, Staphylococci, and Milk & Milk Products
                                                   Tel.: +33 149 772 749
                                                  Adrien.ASSERE@anses.fr

  EURL CPS, Lm, MMP
                        French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety
  European Union Reference Laboratory for
  Coagulase Positive Staphylococci, Listeria
  monocytogenes and Milk and Milk Products
                        23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
French Agency for Food,
               Environmental and Occupational
                        Health Safety

EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
Relations with the government and
                               Parliament
Ministry for ecology                               Ministry for   Ministry for     Ministry for      Ministry for
 and sustainable                                     labour         health       food, agriculture   consumer
  development                                                                      and fisheries       affairs




  5 supervisory ministries
  State Goals & Resources
         Contract




     Vote of the annual grant,
          annual report
                                                                   Parliament
      EURL CPS, Lm, MMP
      European Union Reference Laboratory for
      Coagulase Positive Staphylococci, Listeria
      monocytogenes and Milk and Milk Products
Missions
              Assess nutritional and health            Recommend public health
                   risks and benefits                        measures


     Monitor health                                                 Provide laboratory
        safety                                                      reference missions


        Conduct,
     coordinate and                                                   Provide training
    initiate research                                                and information
         projects                                                    and contribute to
                                                                       public debate
                       Coordinate the R 31                    Authorise marketing of
                       network of scientific                   veterinary medicinal
                          organisations                              products

     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


4
ANSES in brief

 1350 staff
 800 external experts solicited (20 Expert Committees,
  working groups)
 Annual budget: 140 million Euros
 More than 8000 Opinions issued since 1999
 51 National Reference Laboratory mandates, 8 EURL
  mandates
 250 scientific publications per year
 More than 100 doctorate and post-doctorate students



  EURL CPS, Lm, MMP
  European Union Reference Laboratory for
  Coagulase Positive Staphylococci, Listeria
  monocytogenes and Milk and Milk Products
Pôle HQSA
                                  Biological Hazards




                                                                                           Pagot
                                                                                      Chemical Hazards




                                                              Bâtiment C
                                                       Direction/administration


                                                                                  Bâtiment B
                                                                                    LCSV




EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
Laboratory for Food Safety

    • Specialised in biological (raw or process) and
      chemical (environment or process) hazards which
      have an effect on food safety for humans;
    • Activities:
       • Reference
       • Research
       • Surveillance/epidemiosurveillance
       • Scientific and technical expertise


     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


7
EURL CPS, Lm, MMP
                                                                                                                      HAZARDS      European Union Reference Laboratory for
                                                                                                                                   Coagulase Positive Staphylococci
                                                                                                                                   Listeria monocytogenes
                                                                                                                                   Milk and Milk Products
Maisons-Alfort Laboratory for                                                                                            Bacterial toxins
                 Food Safety
                                            Food allergy
                                                                                                                            Mycotoxins
                                            Intolerances
                                                                                      Toxins                               Phycotoxins


                                         Food poisoning                                                                  Animal poisons

   Foodborne                                                                        Poisons                             Vegetal poisons
    diseases
                                                                                                                     Chemical pollutants


                                                                                                                               Bacteria

                                                                                    Invasive                                  Parasites

                                                                                                                                Viruses
                                              Infections
                                                                                                                               Bacteria
                                                                                Non invasive
                                                                                                                              Parasites

  EURL CPS, Lm, MMP
                        French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety
  European Union Reference Laboratory for
  Coagulase Positive Staphylococci, Listeria
  monocytogenes and Milk and Milk Products
                        23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
Maisons-Alfort Food Safety lab in brief

   150 staff
   Between 55000 and 85000 analytical controls each year
   Annual budget: 12,6 million Euros
   10 NRL mandates, 3 EURL mandates
   50 scientific publications per year
   30 technical expertise reports per year
   Around 10 doctorate and post-doctorate students




    EURL CPS, Lm, MMP
    European Union Reference Laboratory for
    Coagulase Positive Staphylococci, Listeria
    monocytogenes and Milk and Milk Products


9
Biological and chemical hazards
 Origin
             o in food
             o generated by processing or preparation
 Missions
             • To improve knowledge on the identification of hazards
             • To develop methods for detecting, characterizing and
               measuring these hazards
             • To investigate why these hazards appear and what causes
               their development
             • To contribute to the implementation of control tools
               (FBO, OCB)
             • To assess the risks associated to these hazards
             • To perform molecular epidemiosurveillance
     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


10
European Union Reference Laboratory
•        Mandate for Listeria monocytogenes
          • Nomination in 2006 (Regulation EC 882/2004
             ammended)
          • EC Regulation 2073/2005 on microbiological criteria
             for foodstuffs




         EURL CPS, Lm, MMP
         European Union Reference Laboratory for
         Coagulase Positive Staphylococci, Listeria
         monocytogenes and Milk and Milk Products


    11
EURL requirements
 • suitably qualified staff with adequate training;
 • equipment and products needed;
 • appropriate administrative infrastructure;
 • ensure confidentiality;
 • sufficient knowledge of international standards and
   practices;
 • take account of research activities at national and EU
   level;
 • trained personnel available for emergency situations
   occurring within the Community.

     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


12
Role of EURL (1)

     • deal with analytical aspects related to the
       corresponding legislation
              –                     Reg. EC 2073/2005 on Microbiological criteria


     • Criteria for Lm checked by Official Reference
       EN ISO methods



      EURL CPS, Lm, MMP
      European Union Reference Laboratory for
      Coagulase Positive Staphylococci, Listeria
      monocytogenes and Milk and Milk Products


13
Food safety criteria from Annex I
                                      (Reg EC 2073/2005)




     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


14
Role of EURL (2)


 To manage a network of National Reference
 Laboratories (NRLs)
   • 35 NRLs: 1/member state
   • Annual workshops
   • Training sessions
   • PT inter-laboratory studies
   • Queries

     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


15
Role of EURL (3)

     To conduct analytical development
       • Internal lab work
       • To develop/to improve reference methods
          for official control




     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


16
Role of EURL (4)

To provide scientific & technical support to EC/DG SANCO
          • Upon the needs
          • Confirmatory analyses
          • Development/implementation of European legislation
                              • Ex 1: preparation of Reg. 2073/2005
                              • Ex 2: Implementation of Annex II of
                                Reg. 2073/2005
                                                  • Guidelines on challenge tests, durability studies
                                                  • Food classification
          • Participation for DG SANCO to European/international
            technical meetings/activities
                              • Ex: CEN/ISO standardization



     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


17
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
Focus on activities

 • Guidance documents (Lm) to provide detailed
   for the Reg EC 2073/2005
 • Methods:
             – Enhancement of Lm enumeration method
                • filtration
                • reduction of the enrichment step
             – PFGE protocole for Lm
             – EURL Lm DB – PFGE profile database

     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


19
Article 3
                                                  General requirements
 • 2. As necessary, the food business operators responsible for
   the manufacture of the product shall conduct studies in
   accordance with Annex II in order to investigate compliance
   with the criteria throughout the shelf-life. In particular, this
   applies to ready-to-eat foods that are able to support the
   growth of Listeria monocytogenes and that may pose a
   Listeria monocytogenes risk for public health.
 • Food businesses may collaborate in conducting those studies.
 • Guidelines for conducting those studies may be included in
   the guides to good practice referred to in Article 7 of
   Regulation (EC) No 852/2004.

     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


20
Guideline document on shelf-life studies
     for Listeria monocytogenes in RTE foods
 • On demand of DG SANCO
 • Written in collaboration with representatives
   of 7 National Reference Laboratories for
   Listeria monocytogenes (BE, NL, IE, NO, RO,
   SE, UK) – version 2 (Nov 2008)
 • Revising after 4 years of practices – new
   members (SK, PT)


     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


21
2010-11 EU baseline study in RTE:
                              opportunity for Typing database
 •      Taken part to EFSA plans
 •      Opportunity for subtyping the strains
 •      NRLs in charge of collecting the strains
 •      Collaboration of the NRLs to type the strains
 •      1st use of the EURL database
 •      Work to be transfered to EFSA


     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


22
Article 5
            Specific rules for testing and sampling
 • Food business operators manufacturing
   ready-to-eat foods, which may pose a Listeria
   monocytogenes risk for public health, shall
   sample the processing areas and equipment
   for Listeria monocytogenes as part of their
   sampling scheme.




     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


23
Guidelines on sampling the food
        processing area and equipment for the
         detection of Listeria monocytogenes
 •      Enquiry
 •      Document drafted by a WG
 •      137 respondents from 15 EU MSs
 •      Presented after by my colleague Léna BARRE




     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


24
Role of NRL

 • Very similar to EURL, but at National level
 1. To manage the network of laboratories
    approved by the Compentent Authority in to
    perform official control analyses
 2. To perform analytical development
 3. Ensure dissemination of information
 4. Provide scientific and technical assistance to
    the competent authority
     EURL CPS, Lm, MMP
     European Union Reference Laboratory for
     Coagulase Positive Staphylococci, Listeria
     monocytogenes and Milk and Milk Products


25                                                   B. Lombard   07-03-2012

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"Overview of the European Union reference laboratory (eu rl) for Listeria monocytogenes (eurl) activities" - Adrien Assere (ANSES, Frankreich)

  • 1. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci Listeria monocytogenes Milk and Milk Products Maisons-Alfort Laboratory for Food Safety Overview of the European Union Reference Laboratory (EURL) for Listeria monocytogenes activities in Anses Laboratory Adrien ASSERE Deputy manager of EU reference Laboratories for Listeria monocytogenes, Staphylococci, and Milk & Milk Products Tel.: +33 149 772 749 Adrien.ASSERE@anses.fr EURL CPS, Lm, MMP French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
  • 2. French Agency for Food, Environmental and Occupational Health Safety EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  • 3. Relations with the government and Parliament Ministry for ecology Ministry for Ministry for Ministry for Ministry for and sustainable labour health food, agriculture consumer development and fisheries affairs 5 supervisory ministries State Goals & Resources Contract Vote of the annual grant, annual report Parliament EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  • 4. Missions Assess nutritional and health Recommend public health risks and benefits measures Monitor health Provide laboratory safety reference missions Conduct, coordinate and Provide training initiate research and information projects and contribute to public debate Coordinate the R 31 Authorise marketing of network of scientific veterinary medicinal organisations products EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 4
  • 5. ANSES in brief  1350 staff  800 external experts solicited (20 Expert Committees, working groups)  Annual budget: 140 million Euros  More than 8000 Opinions issued since 1999  51 National Reference Laboratory mandates, 8 EURL mandates  250 scientific publications per year  More than 100 doctorate and post-doctorate students EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  • 6. Pôle HQSA Biological Hazards Pagot Chemical Hazards Bâtiment C Direction/administration Bâtiment B LCSV EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  • 7. Laboratory for Food Safety • Specialised in biological (raw or process) and chemical (environment or process) hazards which have an effect on food safety for humans; • Activities: • Reference • Research • Surveillance/epidemiosurveillance • Scientific and technical expertise EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 7
  • 8. EURL CPS, Lm, MMP HAZARDS European Union Reference Laboratory for Coagulase Positive Staphylococci Listeria monocytogenes Milk and Milk Products Maisons-Alfort Laboratory for Bacterial toxins Food Safety Food allergy Mycotoxins Intolerances Toxins Phycotoxins Food poisoning Animal poisons Foodborne Poisons Vegetal poisons diseases Chemical pollutants Bacteria Invasive Parasites Viruses Infections Bacteria Non invasive Parasites EURL CPS, Lm, MMP French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr
  • 9. Maisons-Alfort Food Safety lab in brief  150 staff  Between 55000 and 85000 analytical controls each year  Annual budget: 12,6 million Euros  10 NRL mandates, 3 EURL mandates  50 scientific publications per year  30 technical expertise reports per year  Around 10 doctorate and post-doctorate students EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 9
  • 10. Biological and chemical hazards Origin o in food o generated by processing or preparation Missions • To improve knowledge on the identification of hazards • To develop methods for detecting, characterizing and measuring these hazards • To investigate why these hazards appear and what causes their development • To contribute to the implementation of control tools (FBO, OCB) • To assess the risks associated to these hazards • To perform molecular epidemiosurveillance EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 10
  • 11. European Union Reference Laboratory • Mandate for Listeria monocytogenes • Nomination in 2006 (Regulation EC 882/2004 ammended) • EC Regulation 2073/2005 on microbiological criteria for foodstuffs EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 11
  • 12. EURL requirements • suitably qualified staff with adequate training; • equipment and products needed; • appropriate administrative infrastructure; • ensure confidentiality; • sufficient knowledge of international standards and practices; • take account of research activities at national and EU level; • trained personnel available for emergency situations occurring within the Community. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 12
  • 13. Role of EURL (1) • deal with analytical aspects related to the corresponding legislation – Reg. EC 2073/2005 on Microbiological criteria • Criteria for Lm checked by Official Reference EN ISO methods EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 13
  • 14. Food safety criteria from Annex I (Reg EC 2073/2005) EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 14
  • 15. Role of EURL (2) To manage a network of National Reference Laboratories (NRLs) • 35 NRLs: 1/member state • Annual workshops • Training sessions • PT inter-laboratory studies • Queries EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 15
  • 16. Role of EURL (3) To conduct analytical development • Internal lab work • To develop/to improve reference methods for official control EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 16
  • 17. Role of EURL (4) To provide scientific & technical support to EC/DG SANCO • Upon the needs • Confirmatory analyses • Development/implementation of European legislation • Ex 1: preparation of Reg. 2073/2005 • Ex 2: Implementation of Annex II of Reg. 2073/2005 • Guidelines on challenge tests, durability studies • Food classification • Participation for DG SANCO to European/international technical meetings/activities • Ex: CEN/ISO standardization EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 17
  • 18. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products
  • 19. Focus on activities • Guidance documents (Lm) to provide detailed for the Reg EC 2073/2005 • Methods: – Enhancement of Lm enumeration method • filtration • reduction of the enrichment step – PFGE protocole for Lm – EURL Lm DB – PFGE profile database EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 19
  • 20. Article 3 General requirements • 2. As necessary, the food business operators responsible for the manufacture of the product shall conduct studies in accordance with Annex II in order to investigate compliance with the criteria throughout the shelf-life. In particular, this applies to ready-to-eat foods that are able to support the growth of Listeria monocytogenes and that may pose a Listeria monocytogenes risk for public health. • Food businesses may collaborate in conducting those studies. • Guidelines for conducting those studies may be included in the guides to good practice referred to in Article 7 of Regulation (EC) No 852/2004. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 20
  • 21. Guideline document on shelf-life studies for Listeria monocytogenes in RTE foods • On demand of DG SANCO • Written in collaboration with representatives of 7 National Reference Laboratories for Listeria monocytogenes (BE, NL, IE, NO, RO, SE, UK) – version 2 (Nov 2008) • Revising after 4 years of practices – new members (SK, PT) EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 21
  • 22. 2010-11 EU baseline study in RTE: opportunity for Typing database • Taken part to EFSA plans • Opportunity for subtyping the strains • NRLs in charge of collecting the strains • Collaboration of the NRLs to type the strains • 1st use of the EURL database • Work to be transfered to EFSA EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 22
  • 23. Article 5 Specific rules for testing and sampling • Food business operators manufacturing ready-to-eat foods, which may pose a Listeria monocytogenes risk for public health, shall sample the processing areas and equipment for Listeria monocytogenes as part of their sampling scheme. EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 23
  • 24. Guidelines on sampling the food processing area and equipment for the detection of Listeria monocytogenes • Enquiry • Document drafted by a WG • 137 respondents from 15 EU MSs • Presented after by my colleague Léna BARRE EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 24
  • 25. Role of NRL • Very similar to EURL, but at National level 1. To manage the network of laboratories approved by the Compentent Authority in to perform official control analyses 2. To perform analytical development 3. Ensure dissemination of information 4. Provide scientific and technical assistance to the competent authority EURL CPS, Lm, MMP European Union Reference Laboratory for Coagulase Positive Staphylococci, Listeria monocytogenes and Milk and Milk Products 25 B. Lombard 07-03-2012