"Listerien-Referenzlabor: Akademischer Luxus oder Public Health Erfordernis?", 19. Oktober 2012 (AGES, Graz) Das Nationale Listerien-Referenzlabor ist innerhalb der AGES an das "Zentrum für lebensmittelbedingte Infektionskrankheiten" / Institut für medizinische Mikrobiologie und Hygiene in Graz übersiedelt. Zum Auftakt am neuen Standort veranstaltete die AGES dieses Fachgespräch, bei dem verschiedene Aspekte in Zusammenhang mit dem potentiellen Auftreten von Listerien aktuell beleuchtet wurden. Erstmals wurden zudem die neuen Richtlinien des Europäischen Referenzlabors (EU-RL) für Listeria monocytogenes zur Harmonisierung der Probennahme vorgestellt. Dokumentation der Fachtagung mit Präsentationen: http://www.ages.at/ages/ages-akademie/stakeholderveranstaltungen/graz-listerien-referenzlabor/

1. EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci
Listeria monocytogenes
Milk and Milk Products
Maisons-Alfort Laboratory for
Food Safety
Overview of the European Union Reference
Laboratory (EURL) for Listeria monocytogenes
activities in Anses Laboratory
Adrien ASSERE
Deputy manager of EU reference Laboratories for
Listeria monocytogenes, Staphylococci, and Milk & Milk Products
Tel.: +33 149 772 749
Adrien.ASSERE@anses.fr
EURL CPS, Lm, MMP
French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr

2. French Agency for Food,
Environmental and Occupational
Health Safety
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products

3. Relations with the government and
Parliament
Ministry for ecology Ministry for Ministry for Ministry for Ministry for
and sustainable labour health food, agriculture consumer
development and fisheries affairs
5 supervisory ministries
State Goals & Resources
Contract
Vote of the annual grant,
annual report
Parliament
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products

4. Missions
Assess nutritional and health Recommend public health
risks and benefits measures
Monitor health Provide laboratory
safety reference missions
Conduct,
coordinate and Provide training
initiate research and information
projects and contribute to
public debate
Coordinate the R 31 Authorise marketing of
network of scientific veterinary medicinal
organisations products
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
4

5. ANSES in brief
 1350 staff
 800 external experts solicited (20 Expert Committees,
working groups)
 Annual budget: 140 million Euros
 More than 8000 Opinions issued since 1999
 51 National Reference Laboratory mandates, 8 EURL
mandates
 250 scientific publications per year
 More than 100 doctorate and post-doctorate students
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products

6. Pôle HQSA
Biological Hazards
Pagot
Chemical Hazards
Bâtiment C
Direction/administration
Bâtiment B
LCSV
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products

7. Laboratory for Food Safety
• Specialised in biological (raw or process) and
chemical (environment or process) hazards which
have an effect on food safety for humans;
• Activities:
• Reference
• Research
• Surveillance/epidemiosurveillance
• Scientific and technical expertise
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
7

8. EURL CPS, Lm, MMP
HAZARDS European Union Reference Laboratory for
Coagulase Positive Staphylococci
Listeria monocytogenes
Milk and Milk Products
Maisons-Alfort Laboratory for Bacterial toxins
Food Safety
Food allergy
Mycotoxins
Intolerances
Toxins Phycotoxins
Food poisoning Animal poisons
Foodborne Poisons Vegetal poisons
diseases
Chemical pollutants
Bacteria
Invasive Parasites
Viruses
Infections
Bacteria
Non invasive
Parasites
EURL CPS, Lm, MMP
French Agency for Food, Environmental and Occupational Health Safety – Maisons-Alfort Laboratory for Food Safety
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
23, avenue du Général de Gaulle - F94706 Maisons-Alfort Cedex - Telephone: + 33 149 771 300 - Fax: + 33 143 689 762 - www.anses.fr

9. Maisons-Alfort Food Safety lab in brief
 150 staff
 Between 55000 and 85000 analytical controls each year
 Annual budget: 12,6 million Euros
 10 NRL mandates, 3 EURL mandates
 50 scientific publications per year
 30 technical expertise reports per year
 Around 10 doctorate and post-doctorate students
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
9

10. Biological and chemical hazards
Origin
o in food
o generated by processing or preparation
Missions
• To improve knowledge on the identification of hazards
• To develop methods for detecting, characterizing and
measuring these hazards
• To investigate why these hazards appear and what causes
their development
• To contribute to the implementation of control tools
(FBO, OCB)
• To assess the risks associated to these hazards
• To perform molecular epidemiosurveillance
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
10

11. European Union Reference Laboratory
• Mandate for Listeria monocytogenes
• Nomination in 2006 (Regulation EC 882/2004
ammended)
• EC Regulation 2073/2005 on microbiological criteria
for foodstuffs
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
11

12. EURL requirements
• suitably qualified staff with adequate training;
• equipment and products needed;
• appropriate administrative infrastructure;
• ensure confidentiality;
• sufficient knowledge of international standards and
practices;
• take account of research activities at national and EU
level;
• trained personnel available for emergency situations
occurring within the Community.
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
12

13. Role of EURL (1)
• deal with analytical aspects related to the
corresponding legislation
– Reg. EC 2073/2005 on Microbiological criteria
• Criteria for Lm checked by Official Reference
EN ISO methods
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
13

14. Food safety criteria from Annex I
(Reg EC 2073/2005)
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
14

15. Role of EURL (2)
To manage a network of National Reference
Laboratories (NRLs)
• 35 NRLs: 1/member state
• Annual workshops
• Training sessions
• PT inter-laboratory studies
• Queries
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
15

16. Role of EURL (3)
To conduct analytical development
• Internal lab work
• To develop/to improve reference methods
for official control
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
16

17. Role of EURL (4)
To provide scientific & technical support to EC/DG SANCO
• Upon the needs
• Confirmatory analyses
• Development/implementation of European legislation
• Ex 1: preparation of Reg. 2073/2005
• Ex 2: Implementation of Annex II of
Reg. 2073/2005
• Guidelines on challenge tests, durability studies
• Food classification
• Participation for DG SANCO to European/international
technical meetings/activities
• Ex: CEN/ISO standardization
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
17

18. EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products

19. Focus on activities
• Guidance documents (Lm) to provide detailed
for the Reg EC 2073/2005
• Methods:
– Enhancement of Lm enumeration method
• filtration
• reduction of the enrichment step
– PFGE protocole for Lm
– EURL Lm DB – PFGE profile database
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
19

20. Article 3
General requirements
• 2. As necessary, the food business operators responsible for
the manufacture of the product shall conduct studies in
accordance with Annex II in order to investigate compliance
with the criteria throughout the shelf-life. In particular, this
applies to ready-to-eat foods that are able to support the
growth of Listeria monocytogenes and that may pose a
Listeria monocytogenes risk for public health.
• Food businesses may collaborate in conducting those studies.
• Guidelines for conducting those studies may be included in
the guides to good practice referred to in Article 7 of
Regulation (EC) No 852/2004.
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
20

21. Guideline document on shelf-life studies
for Listeria monocytogenes in RTE foods
• On demand of DG SANCO
• Written in collaboration with representatives
of 7 National Reference Laboratories for
Listeria monocytogenes (BE, NL, IE, NO, RO,
SE, UK) – version 2 (Nov 2008)
• Revising after 4 years of practices – new
members (SK, PT)
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
21

22. 2010-11 EU baseline study in RTE:
opportunity for Typing database
• Taken part to EFSA plans
• Opportunity for subtyping the strains
• NRLs in charge of collecting the strains
• Collaboration of the NRLs to type the strains
• 1st use of the EURL database
• Work to be transfered to EFSA
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
22

23. Article 5
Specific rules for testing and sampling
• Food business operators manufacturing
ready-to-eat foods, which may pose a Listeria
monocytogenes risk for public health, shall
sample the processing areas and equipment
for Listeria monocytogenes as part of their
sampling scheme.
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
23

24. Guidelines on sampling the food
processing area and equipment for the
detection of Listeria monocytogenes
• Enquiry
• Document drafted by a WG
• 137 respondents from 15 EU MSs
• Presented after by my colleague Léna BARRE
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
24

25. Role of NRL
• Very similar to EURL, but at National level
1. To manage the network of laboratories
approved by the Compentent Authority in to
perform official control analyses
2. To perform analytical development
3. Ensure dissemination of information
4. Provide scientific and technical assistance to
the competent authority
EURL CPS, Lm, MMP
European Union Reference Laboratory for
Coagulase Positive Staphylococci, Listeria
monocytogenes and Milk and Milk Products
25 B. Lombard 07-03-2012