Gloves are commonly used barriers that protect healthcare providers and patients from transmitting infections. Gloves are made of latex rubber and cannot be reused after becoming contaminated. There are two main methods for applying sterile gloves - open and closed. The open method is used outside the operating room and involves 13 steps to maintain sterility while applying and removing gloves with the hands. Proper gloving and handwashing after removal helps reduce transmission of microorganisms between patients and providers.