6th International Scientific Conference on Applied Sciences and Engineering 26-27 January, 2016 Bayview Hotel Singapore

1. Abstract of Applied Sciences and Engineering, 2016, Vol.6
DOI: 10.18488/journal.1001/2016.6/1001.6
6th
International Scientific Conference on Applied
Sciences and Engineering
26-27 January, 2016
Bayview Hotel Singapore
Conference Website: www.scihost.org
4
Paper ID: 14/16/ 6
th
ISCASE
One-Step Synthesis of Gold Nanoparticles Stabilized by
Oligonucleotides
Taotao Li1
--- Zunliang Wang2
--- Xianbo Mou3
--- Nongyue He4
11,2,3,4
State Key Laboratory of Bioelectronics, School of Biological Science and Medical
Engineering, Southeast University, Nanjing 210096, China
Abstract
DNA templated metal nanoparticles have attracted a lot of interest and have been
widely applied in biomedical fields. Silver, platinum, palladium and copper
nanostructure templated by DNA have been widely reported. While the synthesis of
DNA templated gold nanoparticles (Au NPs) was limited. In this article, we presented a
novel one-step method for synthesizing Au NPs stabilized by oligonucleotides. UV
absorption spectroscopy showed that the formation of Au NPs was associated with pH
value, DNA sequence, and DNA concentration. Under neutral pH condition, the UV
absorption spectra of Au NPs showed that the peak width was narrow. The blue-shift
(from about 540 to 524 nm) of surface plasmon resonance (SPR) peak occurred when
Au NPs formed under higher pH (9.6) condition comparing with neutral pH condition.
Slight red-shift of the SPR peak occurred with increase in concentration of
oligonucleotides. For C10 oligonucleotide stabilized Au NPs, the peak width of UV
absorption spectra was wider than A10 and T10 oligonucleotide stabilized Au NPs,
especially under lower pH value and lower oligonucleotide concentration. The wider
peak width and red-shift were due to aggregation of Au NPs which can be proved by
SEM image.
Keywords: Au NPs, Oligonucleotides, One-step synthesis