This document summarizes Angela Sanchez's research on how temperature-regulated genes affect aging in C. elegans. Key findings include:
- Many proteostasis and protein synthesis genes are differentially expressed between 15°C and 25°C.
- Inhibition of certain temperature-regulated proteostasis genes, like cpr-7 and egl-45, causes earlier onset of paralysis at 15°C but not 25°C.
- A neuromuscular exam showed locomotion defects, especially in backward movement, precede paralysis upon proteostasis gene inhibition. This suggests the dysfunction starts in motor neurons.
- Motor neurons innervating the posterior half have fewer synaptic connections, which
RNA splicing mutations and human disease: Pompe disease - Emanuele Buratti
Convegno del 25 novembre "Diagnosi e management della glicogenosi tipo 2" - Centro di coordinamento regionale malattie rare FVG
RNA splicing mutations and human disease: Pompe disease - Emanuele Buratti
Convegno del 25 novembre "Diagnosi e management della glicogenosi tipo 2" - Centro di coordinamento regionale malattie rare FVG
Activity-dependent transcriptional dynamics in mouse primary cortical and hum...Darya Vanichkina
Poster I presented at Lorne Genome 2012. Subsequently formed part of the paper
Barry G, Briggs JA, Vanichkina DP, Poth EM, Beveridge NJ, Ratnu VS, Nayler SP, Nones K, Hu J, Bredy TW, Nakagawa S, Rigo F, Taft RJ, Cairns MJ, Blackshaw S, Wolvetang EJ, Mattick JS (2013). The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Molecular psychiatry doi: 10.1038/mp.2013.45
Duplicate of http://figshare.com/articles/Activity_dependent_transcriptional_dynamics_in_mouse_primary_cortical_and_human_iPS_derived_neurons/978468
This presentation describes the role of electrophsyiology in treatment of deafferent neuropathic pain during microcoagulation of dorsal root entry zone.
Bioinformática y supercomputación. Razones para hacerse bioinformático en la UMAM. Gonzalo Claros
¿En qué consiste la bioinformática? ¿Cómo puedo especializarme? ¿Dónde? Capacidad de supercomputación en la UMA. Recientes logros bioinformáticos relacionados con la medicina y con la ciencia en general, muchos de ellos realizados por equipos de la UMA.
Noncoding RNAs in Cardiovascular Disease – Potential as Biomarkers and MoreQIAGEN
Cardiovascular diseases (CVD) are the leading cause of death worldwide, and are therefore the subject of intense, urgent research. Biomarkers could help physicians diagnose heart diseases early, for example, and better therapies could improve survival or healing following events like myocardial infarction. Small noncoding RNAs called microRNAs have recently stepped into the spotlight as circulating biomarkers for a number of diseases, and may also have utility in someday treating CVD more effectively. In this slide deck, we discuss why and how microRNAs are being investigated as biomarkers for CVD, as well as examining some recent findings in the field. Check it out to find out how scientists are investigating noncoding RNA involvement in CVD and how you can do the same in your laboratory!
Results of DNA Horses before Columbus research by geneticist Alessandro AchilliRuben LLumihucci
The entire horse mtDNA was amplified in 11 overlapping PCR fragments, using a set of oligonucleotides with matching annealing temperatures (Table S10). Oligonucleotides were checked (through GenBank BLAST) in order to avoid amplification of nuclear insertions of mitochondrial sequences (numts) (1). After PCR, the fragments were purified using the ExoSAP-IT® enzymatic system (Exonuclease I and Shrimp Alkaline Phosphatase, GE Healthcare) and standard dideoxysequencing was performed by using a set of 33 nested primers (Table S11) specifically designed for this protocol. An ABI 3730 sequencer with 96 capillaries was employed for separation of the sequencing ladders. Complete sequences were aligned, assembled, and compared using the program Sequencher 4.9 (Gene Codes). Traces were generally of excellent quality and there was extensive overlap between reads with most observed mutations determined by at least two independent sequencing reactions. At least two independent operators read each sequence and any potentially ambiguous base call was tested by additional and independent PCR and sequencing reactions.
A novel rlimrnf12 variant disrupts protein stability and function to cause se...DanielalvarezCogollo
A novel RLIM/RNF12 variant disrupts protein stability and function to cause severe Tonne–Kalscheuer syndrome
Francisco Bustos, Carmen Espejo-Serrano, Anna Segarra-Fas, Rachel Toth, Alison J. Eaton, Kristin D. Kernohan, Meredith J. Wilson, Lisa G. Riley.
Published online 2021 May 5
presented by: Joaquin Aguirre and Daniela Álvarez
Universidad Pontificia Bolivariana - Molecular Biology
Activity-dependent transcriptional dynamics in mouse primary cortical and hum...Darya Vanichkina
Poster I presented at Lorne Genome 2012. Subsequently formed part of the paper
Barry G, Briggs JA, Vanichkina DP, Poth EM, Beveridge NJ, Ratnu VS, Nayler SP, Nones K, Hu J, Bredy TW, Nakagawa S, Rigo F, Taft RJ, Cairns MJ, Blackshaw S, Wolvetang EJ, Mattick JS (2013). The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Molecular psychiatry doi: 10.1038/mp.2013.45
Duplicate of http://figshare.com/articles/Activity_dependent_transcriptional_dynamics_in_mouse_primary_cortical_and_human_iPS_derived_neurons/978468
This presentation describes the role of electrophsyiology in treatment of deafferent neuropathic pain during microcoagulation of dorsal root entry zone.
Bioinformática y supercomputación. Razones para hacerse bioinformático en la UMAM. Gonzalo Claros
¿En qué consiste la bioinformática? ¿Cómo puedo especializarme? ¿Dónde? Capacidad de supercomputación en la UMA. Recientes logros bioinformáticos relacionados con la medicina y con la ciencia en general, muchos de ellos realizados por equipos de la UMA.
Noncoding RNAs in Cardiovascular Disease – Potential as Biomarkers and MoreQIAGEN
Cardiovascular diseases (CVD) are the leading cause of death worldwide, and are therefore the subject of intense, urgent research. Biomarkers could help physicians diagnose heart diseases early, for example, and better therapies could improve survival or healing following events like myocardial infarction. Small noncoding RNAs called microRNAs have recently stepped into the spotlight as circulating biomarkers for a number of diseases, and may also have utility in someday treating CVD more effectively. In this slide deck, we discuss why and how microRNAs are being investigated as biomarkers for CVD, as well as examining some recent findings in the field. Check it out to find out how scientists are investigating noncoding RNA involvement in CVD and how you can do the same in your laboratory!
Results of DNA Horses before Columbus research by geneticist Alessandro AchilliRuben LLumihucci
The entire horse mtDNA was amplified in 11 overlapping PCR fragments, using a set of oligonucleotides with matching annealing temperatures (Table S10). Oligonucleotides were checked (through GenBank BLAST) in order to avoid amplification of nuclear insertions of mitochondrial sequences (numts) (1). After PCR, the fragments were purified using the ExoSAP-IT® enzymatic system (Exonuclease I and Shrimp Alkaline Phosphatase, GE Healthcare) and standard dideoxysequencing was performed by using a set of 33 nested primers (Table S11) specifically designed for this protocol. An ABI 3730 sequencer with 96 capillaries was employed for separation of the sequencing ladders. Complete sequences were aligned, assembled, and compared using the program Sequencher 4.9 (Gene Codes). Traces were generally of excellent quality and there was extensive overlap between reads with most observed mutations determined by at least two independent sequencing reactions. At least two independent operators read each sequence and any potentially ambiguous base call was tested by additional and independent PCR and sequencing reactions.
A novel rlimrnf12 variant disrupts protein stability and function to cause se...DanielalvarezCogollo
A novel RLIM/RNF12 variant disrupts protein stability and function to cause severe Tonne–Kalscheuer syndrome
Francisco Bustos, Carmen Espejo-Serrano, Anna Segarra-Fas, Rachel Toth, Alison J. Eaton, Kristin D. Kernohan, Meredith J. Wilson, Lisa G. Riley.
Published online 2021 May 5
presented by: Joaquin Aguirre and Daniela Álvarez
Universidad Pontificia Bolivariana - Molecular Biology
KDM5 epigenetic modifiers as a focus for drug discoveryChristopher Wynder
A summary presentation of my scientific work.
My laboratory focused on an enzyme KDM5b (aka PLU-1, JARID1b) that was widely expressed during development and played a key role in progression of breast cancer through HER-2.
My lab focused on understanding the key biochemical activity of the enzyme through dissecting the proteomic and genomic interactors.
Our results were confirmed through the use of ES cells, adult stem cells and mouse models.
Much of this work remains unpublished, please contact me for more information and/or access to any reagents that I still have as part of this work.
crwynder@gmail.com
The discovery of the nuclear factor TDP-43 involvement in neurodegenerative disease has increased significantly the general interest on the characteristics of this protein. The aberrant localization and aggregation of TDP-43 in affected tissues coupled with the tight auto regulation of TDP 43 cellular levels has suggested novel pathways for neurodegeneration. TDP 43 is predominantly a nuclear protein that shuttles between nucleus and cytoplasm. In disease neurons TDP 43 mislocalize to cytoplasmic inclusions with devastating consequences on neuronal survival. These cytoplasmic aggregation disrupts the TDP-43 control of its own cellular level. In fact autoregulation is mediated byan unusual splicing event in the 3’UTR of its pre mRNA for which is essentiial the presence of TDP 43 in the nucleus. In addition animal models and highthroughput assays have recently highlighted the role played by this protein in the regulation of hundreds of nuclear and cytoplasmic RNA transcripts, many of them belonging to key genes for neuronal metabolism. A model has been developed to study the determinants of the aggregation process and the impact of the latter on neuronal function. Animal models of the disease have been developed in different species mainly mice and flies.
Translational Genomics and Prostate Cancer: Meet the NGS Experts Series Part 2QIAGEN
Advanced prostate cancer is highly heterogeneous but this inter-patient heterogeneity has until recently not been understood. We have through an international research effort dissected the molecular landscape of advanced castration resistant prostate, elucidating key molecular targets in this group of diseases. We have also shown that PARP inhibitors have antitumor activity against a significant proportion of these cancers, mainly in men whose cancers harbor DNA repair defects.
Rapid and real-time diagnosis of Laem-Singh virus using a portable Real-Amp T...Narong Arunrut
The most prominent of growth-retarded in black tiger shrimp (Penaeus monodon) is associated with an infection of Laem-Singh virus (LSNV) that has been involved the reduction of annual production volume. To more accurately, rapidly and easily determine its effect on shrimp industries for further control disease, a portable Real-Amp Turbidimeter with real-time detection method was evaluated for LSNV detection.The device exploited the turbidity that produced by-product of magnesium pyrophosphate and displayed turbidity values in real-time on the provided software screen. The results of quantitative can be accomplished by reaction time measuring. The sensitivity of a turbidity detection was comparable to that of the nested RT-PCR, while it was 1000-time more sensitive than RT-PCR. With the use of a portable Real-Amp Turbidimeter. The application of RT-LAMP using a portable Real-Amp Turbidimeter offers a simple and rapid detection platform for LSNV detection in the field assessment. So, This 's awesome technic not only for detection of LSNV but also for many pathogen.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Multi-source connectivity as the driver of solar wind variability in the heli...Sérgio Sacani
The ambient solar wind that flls the heliosphere originates from multiple
sources in the solar corona and is highly structured. It is often described
as high-speed, relatively homogeneous, plasma streams from coronal
holes and slow-speed, highly variable, streams whose source regions are
under debate. A key goal of ESA/NASA’s Solar Orbiter mission is to identify
solar wind sources and understand what drives the complexity seen in the
heliosphere. By combining magnetic feld modelling and spectroscopic
techniques with high-resolution observations and measurements, we show
that the solar wind variability detected in situ by Solar Orbiter in March
2022 is driven by spatio-temporal changes in the magnetic connectivity to
multiple sources in the solar atmosphere. The magnetic feld footpoints
connected to the spacecraft moved from the boundaries of a coronal hole
to one active region (12961) and then across to another region (12957). This
is refected in the in situ measurements, which show the transition from fast
to highly Alfvénic then to slow solar wind that is disrupted by the arrival of
a coronal mass ejection. Our results describe solar wind variability at 0.5 au
but are applicable to near-Earth observatories.
Introduction:
RNA interference (RNAi) or Post-Transcriptional Gene Silencing (PTGS) is an important biological process for modulating eukaryotic gene expression.
It is highly conserved process of posttranscriptional gene silencing by which double stranded RNA (dsRNA) causes sequence-specific degradation of mRNA sequences.
dsRNA-induced gene silencing (RNAi) is reported in a wide range of eukaryotes ranging from worms, insects, mammals and plants.
This process mediates resistance to both endogenous parasitic and exogenous pathogenic nucleic acids, and regulates the expression of protein-coding genes.
What are small ncRNAs?
micro RNA (miRNA)
short interfering RNA (siRNA)
Properties of small non-coding RNA:
Involved in silencing mRNA transcripts.
Called “small” because they are usually only about 21-24 nucleotides long.
Synthesized by first cutting up longer precursor sequences (like the 61nt one that Lee discovered).
Silence an mRNA by base pairing with some sequence on the mRNA.
Discovery of siRNA?
The first small RNA:
In 1993 Rosalind Lee (Victor Ambros lab) was studying a non- coding gene in C. elegans, lin-4, that was involved in silencing of another gene, lin-14, at the appropriate time in the
development of the worm C. elegans.
Two small transcripts of lin-4 (22nt and 61nt) were found to be complementary to a sequence in the 3' UTR of lin-14.
Because lin-4 encoded no protein, she deduced that it must be these transcripts that are causing the silencing by RNA-RNA interactions.
Types of RNAi ( non coding RNA)
MiRNA
Length (23-25 nt)
Trans acting
Binds with target MRNA in mismatch
Translation inhibition
Si RNA
Length 21 nt.
Cis acting
Bind with target Mrna in perfect complementary sequence
Piwi-RNA
Length ; 25 to 36 nt.
Expressed in Germ Cells
Regulates trnasposomes activity
MECHANISM OF RNAI:
First the double-stranded RNA teams up with a protein complex named Dicer, which cuts the long RNA into short pieces.
Then another protein complex called RISC (RNA-induced silencing complex) discards one of the two RNA strands.
The RISC-docked, single-stranded RNA then pairs with the homologous mRNA and destroys it.
THE RISC COMPLEX:
RISC is large(>500kD) RNA multi- protein Binding complex which triggers MRNA degradation in response to MRNA
Unwinding of double stranded Si RNA by ATP independent Helicase
Active component of RISC is Ago proteins( ENDONUCLEASE) which cleave target MRNA.
DICER: endonuclease (RNase Family III)
Argonaute: Central Component of the RNA-Induced Silencing Complex (RISC)
One strand of the dsRNA produced by Dicer is retained in the RISC complex in association with Argonaute
ARGONAUTE PROTEIN :
1.PAZ(PIWI/Argonaute/ Zwille)- Recognition of target MRNA
2.PIWI (p-element induced wimpy Testis)- breaks Phosphodiester bond of mRNA.)RNAse H activity.
MiRNA:
The Double-stranded RNAs are naturally produced in eukaryotic cells during development, and they have a key role in regulating gene expression .
This presentation explores a brief idea about the structural and functional attributes of nucleotides, the structure and function of genetic materials along with the impact of UV rays and pH upon them.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
8. Experimentaldesign of samples for microarray
L1
L2
L3
L4
ImmatureAdult
Development
Total RNA Total RNA
MatureAdult
Visible Developmental Stage
15°C
15°C 25°C
Four synchronizedbiologicalsamples
10. Number of Temperature Regulated Genes
Gene ontology categories
(Curtis, Tavare’, Takano, Sanchez, Larsen, unpublished)
11. Proteostasisgenes differentially expressedby temperature
Higher at 25˚C Lower at 25˚C
Proteostasis: 11 genes Proteostasis : 48 genes
Protein synthesis (0) Protein synthesis (26)
translation
ribosomal biogenesis
rRNA processing
tRNA processing
mRNA processing
rRNA/tRNA processing
rRNA transcription
rRNA/tRNA transcription
ribosome assembly
regulation of translation
proteolysis (4) proteolysis (18)
protein modification process (3) protein modification process (1)
protein folding protein folding
protein glycosylation
(Curtis,Tavare’, Takano, Sanchez Larsen, unpublished)
12. SpecificAim 1: Abbreviated for short talk format
Determine whethergenesthat aretemperature
regulated causelongevity or aging .
a) ValidatebyqPCR
b) Determine theeffect ofRNAi ofselected genesat 15°Cand25°C onlife span
13. Differentiallyexpressed lysosomal protease genes
Gene name Clone ID Description
asp-1 Y39B6A.20 Aspartyl protease
asp-3 H22K11.1 Aspartyl protease
asp-5 F21F8.3 Aspartyl protease
asp-6 F21F8.7 Aspartyl protease
F21F8.4 Aspartyl protease
K10C2.3 Aspartyl protease
C15C8.3 Aspartyl protease
Y39B6A.24 Aspartyl protease
cpr-1 C52E4.1 Cysteine proteinase Cathepsin L
cpr-4 F44C4.3 Cysteine proteinase Cathepsin L
F57F5.1 Cysteine proteinase Cathepsin L
W07B8.1 Cysteine proteinase Cathepsin L
F21D5.2 Cysteine proteinase Cathepsin L
K10C2.1 Serine carboxypeptidases (lysosomal cathepsin A)
K10B2.2 Serine carboxypeptidases (lysosomal cathepsin A)
Only specificgenes are temperature regulated
8regulatedof
19ingenome
5regulatedof
24ingenome
2regulatedof
10ingenome
Classified by active site
14. Differentiallyexpressed protein synthesis genes
Gene name Clone ID Description
eIF-1A ZK856.11 translational initiation
iff-2 F54C9.1 translational initiation
eif-3.I Y74C10AR.1 translational initiation
eIF-2C F20D12.1 translational initiation
eIF-4G M110.4 translational initiation
ife-3 B0348.6 translational initiation
eif-3.G F22B5.2 protein biosynthesis
rpl-24.2 C03D6.8 protein biosynthesis
F53F4.11 protein biosynthesis
eIF-2C C16C10.3 protein biosynthesis
eif-3.E B0511.10 protein biosynthesis
gld-1 T23G11.3 regulation of translation
M28.5 ribosome biogenesis and assembly
K12H4.3 ribosome biogenesis and assembly
Y54E10A.10 ribosome biogenesis and assembly
K07C5.4 ribosome biogenesis and assembly
W01B11.3 ribosome biogenesis and assembly
C43E11.9 ribosome biogenesis and assembly
fib-1 T01C3.7 rRNA processing
ZK430.7 rRNA processing
T07A9.8 rRNA processing
Y53C12B.1 rRNA processing
Only 37of the 589protein synthesis-related
genes on
C.elegans Affymetrixmicroarrray were DE
by temperature
15. *
* *
*
*
*
*
*
* *
* *
*
Verify temperature regulation of lysosomal proteases
qRT-PCRverification
Normalized Ct levels
Temperatureregulation confirmed for 13/15
Range of expression levels: 1000 fold
21. Inhibition oftranslation not universally beneficial
Surprisethat inhibition oftranslation can causeparalysis in middle-aged adults
Translationincreaseslife span
Theseresultsdemonstrate that this is not a universal outcome
Similar to positive/negative/neutral responsestodietary restriction depending on
genotype
Really need to find out what went wrongvery negativeside effect
23. 0
20
40
60
80
100
0 5 10 15 20
PerecentNormal
Locomotion
Days of Adulthood
Empty Vector
cpr-7
0
20
40
60
80
100
0 5 10 15 20
PercentNormalVulva
Days of Adulthood
Empty Vector
egl-45
rpc-1
nol-5
F14B4.3
ifg-1
RNAi knockdown ofproteostasis genes resultsin defects at 15°C
(Takano S. , LarsenP.etal.) (Sanchez A.,Larsen P.etal.)
Maximum life span 35 days
Therefore2/3 ofadult life spent paralyzed
24. SpecificAim 3
a) Quantifyincidence ofdefects at 15°C
b) Distinguishbetween musclularor andneuronal origin ofthe paralysis
Determine the causeofmidlife paralysis
25. Possible approachesto determine neuronalor musculardysfunction
underlyingparalysis
•Tissuespecific RNAi
•Neuronsresistantto RNAi
•Leakage between tissues
•Neurotransmitterdrugs
•40% of animals died preventing longitudinal studies
•Neuromuscularexam without drugsor transgenics
27. •Feed adult worms RNAi for 8 days
•Transfer40 worms to individual plates
•Score on days 9, 10, 11, 12, 13, 15, and 16
•Blinded and unblinded examiner
•Exam consistsoftail tap, pause,head tap, and scorevulva morphology
•Establishes functionof
Touchreception
Musclefunction
Neuronalfunction
forward and backward motion circuits
switching ability
Experimentaldesign
28. But whatdoes it mean?
Exampleof early defect at 15°C
individual# 18 treated withrpc-1 RNAi
29. How I will present the data
9 10 11 12 13 15 16
Day ofadulthood
30. Individuallongitudinaldata from neuromuscularexam
Control
Control #17 This individual would mistakenly
be scored as effected in a cross-sectional
study rather than never normal
Control #25 This individual was moving
slowly on day 13 and paralyzed by day 16
Control #9 and 11 These individuals slow
down prior to having a backwards defect
31. Individual longitudinal data fromneuromuscularexam: Control
Control
Control #17/18 These individuals develop a
protruding vulva after the reproductive
period is over
32. Defects in backwards movement
precedes paralysis in 70% of cases
SHOWS UP EARLIER AND IN GREATER
FREQUENCY THAN CONTROLS
Individual longitudinal data fromneuromuscularexam: cpr-7
Progressive, similar to neurodegenerative
diseases in humans
33. Individual longitudinal data fromneuromuscularexam: rpc-1
Defects in backwards movement
precedes paralysis in 54% of cases
36. Vulva and locomotion circuits shareneurotransmittersand areadjacent
•Vulval musclesare essential foregg laying
•VC4 and VC5 cholinergic motor neuronsinhibit opening
37. Why is posterior half paralyzed first?
Number of motor neuronsare spread over
length of the animal in the ventral nervecord
Arethe number of synapses different overthe length of the animal?
40. Deducedmechanismof neuraldysfunction
•Factorsnecessaryfor synaptic maintenance or neurotransmissionunder
cool conditions arenot reaching theirdestination
•Motor neuronswith fewer synapsesare sensitive
•Dysfunction startsnearthe tail and progressestoward the head
•Protrusionfromthe vulva is similarly neuronalin origin and occurs
concurrentwith locomotion dysfunction
41. SIGNIFICANCE
Environmental changestrigger beneficial and detrimental responses.By
associating molecular signatureswith life expectancywe can predict
phenotypic outcome in different genotypes and environments.
42. Acknowledgements
Committee MembersPast Larsen Lab Members
CollaboratorsFunding
Dr.Shelly Buffenstein
Dr.Ellen Kraig
Dr.Merry Lindsey
Dr.Jim Lechleiter
Dr.Syuichi Takano
Wendy Lee
Dr.Hal Boylston
Dr.Pamela Larsen
NIA Training Grant
Glenn Medical Foundation
Ellison Medical Foundation
Dr.Simon Tavaré
Dr.Christina Curtis
Editor's Notes
FIX LABELS
FIX LABELS
Explain that we are starting at the beginning (day one chronic response) acute is what saves you in a pinch but chronic is what correlates with lifespan acute is not tolerable long-term
Say specific transcriptional reponse
This doesn’t support the idea that everything is lower and slower at 15
FIX FONT
Say this is the gene name and this is the putatitive mammmalian homologue. Conclusion that this is specific since only a few are regulated.
Say this is the gene name and this is the putatitive mammmalian homologue. Conclusion that this is specific since only a few are regulated.
Once validated justfies functional testing
MARK WHAT LINE IS WHAT CONTROL AND DRUG COLORS. Change to squares and circles for color blind folks. 1 in 4!!!. Make symbols bigger than you think they should be. Some might call this an adaptive change
MARK WHAT LINE IS WHAT CONTROL AND DRUG COLORS. Change to squares and circles for color blind folks. 1 in 4!!!. Make symbols bigger than you think they should be. Some might call this an adaptive change
MARK WHAT LINE IS WHAT CONTROL AND DRUG COLORS. Change to squares and circles for color blind folks. 1 in 4!!!. Make symbols bigger than you think they should be. Some might call this an adaptive change
d
d
These are decrepit (biologist) if you are an MD they are frail.
These are decrepit (biologist) if you are an MD they are frail.
25/ 36 AND IS PROGRESSIVE LIKE ALL OF THE FEARED HUMAN NEURODEGENERATIVE diseases
All are genetically identical with controlled environment
Refined careful examinations
15/28
These are decrepit (biologist) if you are an MD they are frail.
SAY ANALOGY IS THIS NERVE RADIAL NERVE INNERVATING THE THUMB FOR MOTOR CONTROL. How worms are like humans. Pause so that they understand on them if you’ve lost the ability to do something to how these nerves work. What their names are what the names information carries. Say these name carry ventral or dorsal and mean innervate ventral or dorsal. Go over type a/b/d motor neurons front and back etc.emphasize that this is just motorneurons. Not all neurons in the worm.
Cool core temperature – genotype response not universal phenotype outcome. Genetically complex trait of healthy long-term survival.