my work on cell culture

1.  Nutritional requirement
 Growth
 Metabolism
 Serum
 Topoinhibition
 Source substitute
 pH requlation
presented by :
ANMOL FATIMA

2. Nutrients
components in foods that a cell uses to survive and
grow are known as nutrients

3. NUTRITIONAL REQUIREMENT
 For mammalian cell and plant cell culturing
 Amino acids
 (arginine, leucine etc)
 Vitamins
 ( riboflavin, biotin, vitamin B12)
 Inorganic ion
 ( sodium ,potassium, calcium)
 Trace elements
 (molybdenum, copper)
 Monosaccharide
 ( sugar ,glucose, galactose )

4. NUTRITIONAL REQUIREMENT
Hormone
in case of plant cell culturing auxin ,cytokin
, gibberellin, absic acid are required
in case of mammalian cell
culturing somatotrophin, hydrocortison ,prolectin etc
are required

5. NUTRITIONAL REQUIREMENT
 In case of plant cell culturing
 Undefined organic supplement required
For example
protein hydrosylate
Coconut milk
Yeast charcoal

6. GROWTH
 Growth refers to a positive change in size,
and/or maturation, often over a period of time.

7. GROWTH

8. GROWTH
 Characteristic Growth Pattern of Cultured Cells.
Lag phase
The first phase of growth after the culture is
seeded is the lag phase, which is a period of slow
growth when the cells are adapting to the culture
environment and preparing for fast growth.


9.  Exponential phase
 The lag phase is followed by the log phase (i.e.,
“logarithmic” phase) a period where the cells
proliferate exponentially and consume the nutrients
in the growth medium.

10. GROWTH
 Stationary phase
 When all the growth medium is spent (i.e., one or
more of the nutrients is depleted) or when the cells
occupy all of the available substrate, the cells enter
the stationary phase where the proliferation is
greatly reduced or ceases entirely

11. CELL METABOLISM
 Metabolism is sum total of all chemical process
Take place in living cell
Catabolism -breaking down
Anabolism –building up

19. SERUM
 the component of blood that is neither a blood
cell (serum does not contain RBC or WBCs) nor
a clotting factor it is the blood plasma not including
the fibrinogens.

20. SERUM
 SERUM =PLASMA-CLOTTING
FACTOR

21. SERUM
 Serum includes all proteins except blood
clotting(coagulation)andelectrolyte, antibodies, anti
gens, hormones, and any exogenous substances
(e.g., drugs and microorganisms).

22. SERUM
 Serum can derive from different animal like Horese
serum and human serum
 Specific serum use for growth of specific cell type

23. TYPE OF SERUM
 Foetal bovine serum(also known as foetal calf
serum )
 Newborn calf serum
 Donor calf serum
 Horse serum
 Chicken serum
 Human serum

24. TOPOINHIBITION
 Inhibition of cells surface proliferation as the cell
become closely packed on a culture dish they
generally suppressed by the term density
dependent inhibition is called topoinhibition
 The cell would able to receive and transmit signal
to the nucleus both positive and negative signal

25. TOPOINHIBITION

26.  In tissue culture positive signal are provided by
substrate (glass or plastic)
 Negative signal provided by intercellular contacts
topo inhibition

27. TOPOINHIBITION
 Serum may reduce topoinhibition by shielding its
receptors
 Migration of wound would reduce topoinhibition By
reducing intercellular contacts

28. PH REQULATION
 Most normal mammalian cell lines grow well at pH 7.4,
and there is very little variability among different cell
strains.
 However, some transformed cell lines have been shown
to grow better at slightly more acidic environments (pH
7.0–7.4), and
 some normal fibroblast cell lines prefer slightly more
basic environments (pH 7.4–7.7)
 Insect cell lines such as Sf9 and Sf21 grow optimally at
pH 6.2.

29. SOURCE SUBSTITUTE
 thing that takes the place of someone or something
else are called substitute
 Iron salt as a replacement for transferrin
 Transferin is used in culture as an iron transporter
for animal cells however for protein free medium
that do not contain component of animal origin
,transferin can be replace by ferric salt