The document describes a biological wastewater treatment system called the Koningshoeven BioMakery located in La Trappe, Netherlands. The system uses a Metabolic Network Reactor (MNR) technology with 2-3,000 species of organisms to treat wastewater from a nearby beverage factory. The objectives are to combine the MNR with membranes to recover nutrients and water for reuse, and to produce fertilizer from recovered nutrients. Testing shows the MNR is able to reduce key water quality parameters but is sensitive to fluctuations in influent. Lessons learned include the need for closer interaction with the factory and redundant treatment to handle variability.

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Circular solutions for
Water Materials
Relevant data
Lead partners
Relevant sectors
#6.
La Trappe (NL)
La Trappe factory
Capacity: ~360 m3
/day (10,000 PE)
Footprint: 847m2
Value: Water circularity showcase
Beverage industry Municipal sector Space industry
The Koningshoeven BioMakery is a biological
wastewater treatment system based on modular
and functional reactor- based ecological
engineering.
Based upon the principle of water-based urban circularity,
where energy, food, and waste systems are built around
a regenerative and sustainablewater cycle.
Powered by Metabolic Network Reactor (MNR)
technology, which uses 2-3,000 different species of
organisms ranging from bacteria to higher level
organismssuchas plants.

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1. Objectives of the NextGen solutions
 Combine Metabolic Network Reactor (MNR) and membranes
for nutrient and water recovery for fit-for-use industrial use
such as irrigation, bottlewashing or make up water for beer
production
 Use “Bio-makery” for water reuse in decentralized areas
 Carbon, nitrogen and phosphorus recovery, with nutrients
removed from the water converted into fertilizer used to
produce plant or microbial protein

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Technology Evidence Base (TEB).
Initial draft – to be finalised in D1.6
La Trappe
Positioning of demo case within the CE
1. Objectives of the NextGen solutions

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2. New NextGen solutions
Water discharged to the
nearby canal, maintaining
the local water cycle,
preventing drought in the
area. Water is also used
for irrigation.
© 2020, SEMiLLA IPStar
Task 1.2.6 Production of fit-for-purpose water in La Trappe

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French/Italian research station on Antarctica
 Aim to not contaminate pristine environment
• 90%+ recycling of grey water for 10-15 overwintering
scientists
• Semi-autonomous operation for 8 years
• Very robust: limited maintenance required © 2020, SEMiLLA IPStar
2. New NextGen solutions
MELiSSA: Inspiration by Concordia Research Station
Task 1.2.6 Production of fit-for-purpose water in La Trappe

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MELiSSA inspiration: WTUB
De Paepe et al. (2018);
Lindeboom et al. (2020)
Task 1.2.6 Production of fit-for-purpose water in La Trappe
2. New NextGen solutions

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2. New NextGen solutions
Final Scheme of the new Nextgen solution
© 2020, SEMiLLA IPStar
Task 1.4.4. Protein production in Bio-Makeries

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3. Results – MNR Water quality analysis
Case
study
Topic Objectives
Key
Parameters
Influent
Range (mg/l)
Discharge
Limits
(mg/l)
Effluent
Range
(mg/l)
#6
La
Trappe
(NL)
Water
Successful
operation of
brewery
MNR
Effluent is fit
for irrigation
use / aquifer
recharge.
COD 453 – 3829 125 62 – 427
Ammonium 0.08 – 4.6 1 0.01 – 8.3
TN 4.9 – 47 10 4.8 – 24.1
TP 4.5 – 26.1 0.3 1.8 – 17.8
TSS No Data 2 No Data
Chloride 43 – 110 60 60 – 80
Sulphate 20 – 103 60 62 – 159
Note: The MNR on the brewery line demonstrated the ability to reduce most important water quality parameters
below discharge limits under stable influent conditions. However, due to a highly variable influent and operational
difficulties stemming from Covid19, the system is not yet stable and does not meet the discharge limits consistently.
MNR has ability to reduce most important water quality
parameters, but is sensitive to fluctuations in brewery

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MELiSSA Separation systems offline tests
Preliminary tests succesfully performed by
MELiSSA/SEMiLLA IPStar partner Firmus
http://www.fgwrs.mc/en/homepage/
5L Raw brewery
water send to
France
Potable water
quality reached
Ultrafiltration Reverse Osmosis
Larger pilot
experiment is
being planned
3. Results – ‘fit-for-purpose’ water
Task 1.2.6 Production of fit-for-purpose water in La Trappe
118 μS/cm
2400 μS/cm

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 Phase frequency detector (PFD) by Uantwerp
and Matlab model in collaboration with TUDelft
and U Antwerpen
3. Results
PFD and Matlab model of the pilot plant
Task 1.4.4. Protein production in Bio-Makeries

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Photobioreactor technology on La Trappe site
2. Results
Task 1.4.4. Protein production in Bio-Makeries

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5. Lessons learned
 Closer interaction with brewery needed.
 Due to decentralized character highly reliant on too few operators
 Combination with remotely controlled physicochemical treatment
provides redundancy
 Interesting solution that offers opportunity for high-value added product
recovery, but requires large surface area
 Fluctuations may affect quality of biomass
 Operation by highly skilled operators crucial (PD and MSc students)

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2. Lessons learned – new solutions
Local solution to COVID challenges
Collaboration started with Jotem waterbehandeling B.V.
 Construction of WTUB inspired systems on-going
• Scenario 1: Brewery effluent
• Scenario 2: Municipal effluent
• Both scenarios: reuse quality of permeate (QMRA and
QCRA) and concentrate quality for purple bacteria
Task 1.2.6 Production of fit-for-purpose water in La Trappe
(Pilot)
Installation of remotely controlled pilot (capillary NF 400/800 Da) for fit-for-purpose
water 100-150 l/h after MNR from December 1st, 2020

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Containerized Photobioreactor technology on
La Trappe site
2. Lessons learned – new solutions
Task 1.4.4. Protein production in Bio-Makeries
Improved containerized system installed that enables more control and is scalable

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Thank you!
xtGen - Community of Practice
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