In 2003 IFPRI released the results of Next Harvest, a study that compiled and analyzed the first comprehensive database of publically-developed genetically modified crops under development in non-industrialized countries. Since then, several regional and national efforts have been made to update this database and expand Next Harvest findings. Nevertheless, to this day there are no comprehensive data about the state of biotechnology in developing countries, that takes into account both traditional and modern biotechnologies under development by the public and private sector. In particular, in Africa the lack of standardized and uniformly collected data is limiting the ability to assess the overall state of Africa’s agricultural biotechnology capacity and draw policy recommendations regarding countries’ strengths and needs. To begin to fill this gap, IFPRI has started gathering information using standard data collection protocols in four countries in Africa. This study presents the results for Kenya, Nigeria, South Africa, and Uganda and shows the differences and similarities among their state of biotech development. South Africa, clearly the leader in biotech adoption in the continent, is a country where agricultural biotechnology has been mainstreamed in a significant number of agricultural research institutes. Nigeria, on the other hand, has had more difficulties developing and implementing biotechnologies. Kenya and Uganda maintain a solid portfolio of agricultural biotechnology research but still face institutional and human and financial resource limitations. Drawing from the rich data collected, the study identifies the opportunities and challenges and makes policy recommendations to address current limitations.