The document summarizes the Kaushalya Training Program for Skill Development in Biotechnology 2023-24 initiated by GBRC to provide learners with extensive practical knowledge and skills in biotechnology. A total of 12 trainings are planned covering various advanced tools and technologies in biotechnology. The document also provides brief details about upcoming trainings, GBRC news, honours received by the director, invited talks, MOUs signed, student programs, publications and new instruments acquired.
Study on multi-target mechanism of Radix et Rhizoma Rhei (Dahuang) and Semen ...LucyPi1
Abstract Objective: To explore the mechanism of action of Radix et Rhizoma Rhei (Dahuang) (RERR) and Semen Persicae (Taoren) (SP) on adhesive intestinal obstruction (AIO). Methods: The main targets of the active ingredients of RERR and SP were filtered based on the traditional Chinese medicine system pharmacology analysis platform. Cytoscape 3.2.1 was applied to build the ingredient-target network of RERR and SP for AIO. Results: Fifteen active components were predicted from the RERR and SP herb pair, such as aloe-emodin, catechin, rhein, gibberellin (GA) 119, GA120 and GA121. These components were applied to 59 targets mainly involved in many biological processes such as signal transduction, anti-apoptosis, and inflammatory response involved in activating the immune effect. Conclusion: This study proposes the system pharmacology method and identifies the potent combination therapeutic mechanism of RERR and SP for AIO. This strategy will provide a new insight to the study of herb combinations.
Ligand and structure based drug design against antimicrobial resistance induc...Mohit Kumar
Helicobacter pylori is a gram-negative, microaerophilic bacterium found usually in the stomach of a person with chronic gastritis and gastric ulcers. More than 50% of the world’s population harbor H. pylori in their upper gastrointestinal tract. About 85% of people infected with H. pylori never experience symptoms or complications. Individuals with chronic gastritis and infected with H. pylori have a 10 to 20% lifetime risk of developing peptic ulcers, MALT lymphoma, the pathogenesis of gastric cancer and several extra-gastric diseases. No vaccines are developed yet and the bacterial antibiotic resistance has been a growing concern. Helicobacter pylori produce virulence and antibiotic resistance through quorum sensing mechanisms by generating AI-2. Inhibition of quorum sensing would be a novel approach for the effective treatment of antibiotic-resistant strains of H. pylori. Chemical nature of AI-2 is furanosyl borate diester which is generated from 4,5-dihydroxy 2,3-pentanedione (DPD). But there are no synthetic congeners of AI-2 and DPD compounds tested against H. pylori till date. Therefore, it is the aim of the present study to design some potent AI-2 and DPD compounds under the framework of pharmacophore modeling.
The herbicide residue from intensive agricultural
activity provokes environmental disturbances and human health injuries. Among the enzymatic disruptor herbicides, mesotrione is able to inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), which plays a key role in the carotenoid synthesis. Therefore, enzyme-based sensors are innovative options for monitoring herbicides used in agriculture.
Abstract book for the following conferences:
2013 2nd International Conference on Bioinformatics and Biomedical Science (ICBBS 2013)
2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013)
2013 2nd International Conference on Chemical and Process Engineering (ICCPE 2013)
2013 2nd Journal Conference on Environmental Science and Development (JCESD 20132nd)
The conferences was held at Concorde Inn Kuala Lumpur International Airport, Malaysia on 09 June 2013
Study on multi-target mechanism of Radix et Rhizoma Rhei (Dahuang) and Semen ...LucyPi1
Abstract Objective: To explore the mechanism of action of Radix et Rhizoma Rhei (Dahuang) (RERR) and Semen Persicae (Taoren) (SP) on adhesive intestinal obstruction (AIO). Methods: The main targets of the active ingredients of RERR and SP were filtered based on the traditional Chinese medicine system pharmacology analysis platform. Cytoscape 3.2.1 was applied to build the ingredient-target network of RERR and SP for AIO. Results: Fifteen active components were predicted from the RERR and SP herb pair, such as aloe-emodin, catechin, rhein, gibberellin (GA) 119, GA120 and GA121. These components were applied to 59 targets mainly involved in many biological processes such as signal transduction, anti-apoptosis, and inflammatory response involved in activating the immune effect. Conclusion: This study proposes the system pharmacology method and identifies the potent combination therapeutic mechanism of RERR and SP for AIO. This strategy will provide a new insight to the study of herb combinations.
Ligand and structure based drug design against antimicrobial resistance induc...Mohit Kumar
Helicobacter pylori is a gram-negative, microaerophilic bacterium found usually in the stomach of a person with chronic gastritis and gastric ulcers. More than 50% of the world’s population harbor H. pylori in their upper gastrointestinal tract. About 85% of people infected with H. pylori never experience symptoms or complications. Individuals with chronic gastritis and infected with H. pylori have a 10 to 20% lifetime risk of developing peptic ulcers, MALT lymphoma, the pathogenesis of gastric cancer and several extra-gastric diseases. No vaccines are developed yet and the bacterial antibiotic resistance has been a growing concern. Helicobacter pylori produce virulence and antibiotic resistance through quorum sensing mechanisms by generating AI-2. Inhibition of quorum sensing would be a novel approach for the effective treatment of antibiotic-resistant strains of H. pylori. Chemical nature of AI-2 is furanosyl borate diester which is generated from 4,5-dihydroxy 2,3-pentanedione (DPD). But there are no synthetic congeners of AI-2 and DPD compounds tested against H. pylori till date. Therefore, it is the aim of the present study to design some potent AI-2 and DPD compounds under the framework of pharmacophore modeling.
The herbicide residue from intensive agricultural
activity provokes environmental disturbances and human health injuries. Among the enzymatic disruptor herbicides, mesotrione is able to inhibit 4-hydroxyphenylpyruvate dioxygenase (HPPD), which plays a key role in the carotenoid synthesis. Therefore, enzyme-based sensors are innovative options for monitoring herbicides used in agriculture.
Abstract book for the following conferences:
2013 2nd International Conference on Bioinformatics and Biomedical Science (ICBBS 2013)
2013 2nd International Conference on Environment, Energy and Biotechnology (ICEEB 2013)
2013 2nd International Conference on Chemical and Process Engineering (ICCPE 2013)
2013 2nd Journal Conference on Environmental Science and Development (JCESD 20132nd)
The conferences was held at Concorde Inn Kuala Lumpur International Airport, Malaysia on 09 June 2013
Microbial community analysis in anaerobic palm oil mill effluent (pome) waste...eSAT Journals
Microorganisms play a key role in wastewater bio-treatment processes and understanding the microbial community structure is of great importance to improve treatment performance. Denaturing gradient gel electrophoresis (DGGE) was used to monitor succession of the microbial community and thus predominant bands were sequenced to reveal the microbial community composition inside palm oil mill effluent (POME) wastewater.DNA bands from DGGE gels were excised with a sterile blade and placed in 1.5 ml eppendorf tube containing 50 μl deionized water (ddH2O). Tubes were incubated overnight at 4C to elute the DNA. Eluted DNA was purified using QIAquick gel extraction kits (QIAGEN, Inc., Valencia, CA) and was frozen and thawed three times.Microbial DNA successfully excised and purified from DGGE was amplified using polymerase chain reaction (PCR).Five micro liters of the supernatant were used as a template to re-amplify the DNA using 16s rDNA primers,341f (with no GC-clamp) (5'- cct-acg-gga-ggc-agc-ag-3') and reverse(r) primers 907r (5'-ccc-cgt-caa-ttc-att-tga-gtt-t-3'). Amplification was repeated referring to the steps in ‘PCR amplification of 16s rDNA’. PCR products from agarose gels were cut and purified using QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, CA), which were similar to the purification steps after recovery of DNA from DGGE, and sequenced in both directions with the same primers (with no GC-clamp) as used in PCR. Moreover, start-up is an important step in establishing proper community structure in all kinds of biological treatment processes. In anaerobic POME wastewater, 6 sequences of Firmicutes, 5 sequence of Proteobacterium and 2 sequences of Bacteroidetes were found through denaturing gradient gel electrophoresis (DGGE) results. Sequence closely related to Rummeliibacillus suwonensis strain G20 was detected grows at band BE10, BE11, BE12, BE15, BE16, BE17 and BE18. Meanwhile BE25 and BE26 were found at band after treatment process done.Rummeliibacillus suwonensis is an aerobic, Gram-positive, rod shaped, round-spore-forming bacteria which were isolated from aerobic condition. Keywords: Palm oil mill effluent (POME), anaerobic POME, Denaturing gradient gel electrophoresis (DGGE), and microbial community.
A primary screening of antitumor substances was carried out among strains of actinomycetes isolated from the samples of natural substrates of arid zones in the Ile-Balkhash region. Antitumor properties of actinomycetes against Staphylococcus aureus209Р(S. aureus 209P) and its mutants UF-2 and UF-3 were studied using the agar block technique. The diameter of growth inhibition zone was measured after incubation of the test microorganisms at a temperature of 37 °C for 24 hours. 16 strains of actinomycetes (10,2%) from sandy soils and from the plant rhizosphere of the Kapshagai area virtually having no effect or only slightly affecting the growth of wild-type culture.22 strains of actinomycetes (14,0%) from takyrs and takyr-like soils of the Balkhash area had an activity against staphylococcal mutants two or more times higher than against the stock staphylococcal strain.This strains may be the potential producers of antitumor antibiotics.It was established that 24.2% of the strains of actinomycetes may be potential producers of antitumor antibiotics.
Molecular Epidemiology entails the inclusion in epidemiological research of biologic measurement made at the molecular level-and thus is an extension of the increasing use of biologically based measures in epidemiological research (McMichael ,1994).The term ‘molecular epidemiology’ may suggest the existence of a sub-discipline with substantive new research content . Molecular techniques, however, are directed principally at enhancing the measurement of exposure, effect, or susceptibility , and not formulating new etiologic hypotheses. As techniques of refinement and elaboration , the integration of molecular measures into mainstream epidemiologic research can offer higher resolution answers in relation to disease causation.
26 ASBMB TODAY FEBRUARY 2021Discovering an old DoGs’ neMargaritoWhitt221
26 ASBMB TODAY FEBRUARY 2021
Discovering an old DoGs’
new trick
Heterotrimeric G proteins regulate
a variety of signaling pathways that
control cell development and influ-
ence cell morphology via actin/cyto-
skeleton remodeling. There are four
main families of G proteins: Gi/Go,
Gq, Gs and G12/13. Researchers long
have thought that Gs, unlike its family
members, is coupled specifically and
exclusively to adenylyl cyclases.
In a new study published in the
Journal of Biological Chemistry,
Alejandro Castillo–Kauil of the
Center for Research and Advanced
Studies of the National Polytechnic
Institute and collaborators challenge
this dogmatic view by identifying a
new Gs target. Using biochemical,
molecular biological and chemo-
genetic approaches, the researchers
demonstrated that the Gαs subfamily
of G proteins can regulate the activity
of Rho GTPases such as Rho guanine
nucleotide exchange factor, or Rho-
GEF. The interaction identified by the
group activates the small G protein
Cdc42 by Gs-coupled GPCRs, stimu-
lating a rearrangement of the cyto-
skeleton and inducing formation of
fingerlike protrusions called filopodia.
These results provide new insight
into G protein activity and define a
new role for RhoGEF coupling in G
protein function.
DOI: 10.1074/jbc.AC120.015204
A pathogen’s proteins target
mitochondria
The tick-borne pathogen Coxiella
burnetii causes Q fever, or query fever,
a rare flulike disease that can spread
to humans who inhale dust particles
contaminated by infected farm or
CONTINUED FROM PAGE 25
Noninvasive tool provides oral cancer prognosis
Oral squamous cell carcinoma, which affects about 34,000 people
in the U.S. each year, is found in the cells lining the lips and mouth.
Metastasis to the lymph nodes is a sign of disease progression and may
be accompanied by changes in proteolytic activity. During proteolysis,
enzymes cut up proteins into short fragments called peptides. Recent
work suggests that characterizing the sequence and abundance of these
molecules — a method dubbed peptidomics — might provide new in-
sight on cancer biology and in the clinic. In a recent paper in the journal
Molecular & Cellular Proteomics, Leandro Xavier Neves of the Brazil-
ian Biosciences National Laboratory and a team of Brazilian clinicians
and scientists describe their analysis of oral squamous cell carcinoma
patient saliva using peptidomics.
After extracting peptides from saliva samples, the research team ana-
lyzed and compared the peptide content in samples from patients with
and without metastasis to the lymph nodes. They found more than 1,000
uniquely expressed peptides in each group and an additional 1,628 pep-
tides expressed by both groups. A series of statistical analyses identified
77 peptides of particular interest; all of these peptides are overexpressed
in samples from patients with lymph node metastasis, which supports the
hypothesis that proteolytic activity increases ...
Applications of bioinformatics, main by kk sahuKAUSHAL SAHU
Introduction
Goals of Bioinformatics
Bioinformatics & Human Genome
Project
What can we do using bioinformatics ?
Applications of bioinformatics in various fields
1) Medicine
2) Evolutionary studies
3) Agriculture
4) Microbiology
5) Biotechnology
Conclusion
References
Pharmacokinetic Properties of Biomass-extracted Substances Isolated by Green ...Michal Jablonsky
According to the literature, approximately 41 nutraceutical compounds have been isolated from different types of biomass using green solvents. It is important to collect information on the pharmacokinetic properties of the nutraceutical substances from biomass isolated according to the published papers. The pharmacokinetic properties of the bioactive substances extracted by green solvents, such as the molecular weight, logP, AlogP, H-bond acceptor, H-bond donor, total polar surface area, atom molar refractivity, number of rotatable bonds, number of atoms, rotatable bond count, number of rigid bonds, number of atom rings, and number of H-bonds, were calculated with a drug-likeness tool. In practical terms, the original and most well-known Lipinski's Rule of Five (Ro5) was applied to 28 substances, namely 3-hydroxytyrosol; apigenin; artemisinin; bergapten; bilobalide; biochanin A; caffeic Acid; caffeoylmalic acid; catechins; cinnamic acid; curcumin; daidzei; daidzin; epicatechin; gallic acid; genistein; ginkgolide A; ginkgolide B; levofloxacin; luteolin; naringenin; p-coumaric acid; protocatechuic acid; psoralen; quercetin; trans-ferulic acid; tyrosol, and vanillin.
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...ijtsrd
"Introduction Betel quid BQ chewing, a habit practiced in Eastern and North Eastern part of India, has known to be associated with cancer of the oral or buccal cavity. BQ is also one of the common mood elevating substances among Indian population. The BQ is a mixture of areca nut Areca catechu , catechu Acacia catechu and slaked lime calcium oxide and calcium hydroxide wrapped in a betel leaf Piper betel .BQ products have been classified by the International Agency for Research on Cancer IARC as group I human carcinogens . Glutathione peroxidise GPx , one of the major enzymatic antioxidant defence system, responsible for scavenging free radicals. Antioxidant enzymes catalyze decomposition of ROS. Overall balance between production and removal of ROS may be more important in various cancers including OSCC Oral squamous cell carcinoma or oral cancer. Methods In this study subjects were screened from Department of Oral and Maxillofacial surgery andE.N.T. of Ramakrishna Mission Seva Pratishthan Hospital RKMSP , Kolkata and different areas of West Bengal and North Eastern states of India. Quantitative in vitro determination of glutathione peroxidase activities in whole blood were estimated manually with 0.05 ml whole blood. The samples were assayed by UV Visible Spectrophotometer SPECORD 50 PLUS at a wavelength of 340nm. Results Most of the subjects had betel quid chewing habit. Glutathione peroxidase values are higher in healthy control than Cancer cases and Pre cancer with betel quid chewing habit, which is statistically significant. Conclusion Reactive oxygen species are generated due to slaked lime, one of the important constituents of betel quid which can modulate the oral pathology and promote carcinogenesis. Aniket Adhikari | Madhusnata De ""Study of Glutathione Peroxidase (GPX) Activity Among Betel Quid Chewers of Indian Population"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21619.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21619/study-of-glutathione-peroxidase-gpx-activity-among-betel-quid-chewers-of-indian-population/aniket-adhikari"
PAMAM/5-fluorouracil drug conjugate for targeting E6 and E7 oncoproteins in c...Arun kumar
In the present study, poly(amidoamine)/5-fluorouracil (PAMAM/5-FU) was prepared and used as
a conjugate system for delivering drugs to target E6 and E7 oncoproteins, which are predominant in
cervical cancers. Specifically, molecular docking analysis was used to investigate the interaction
between the PAMAM/5-FU and E6/E7 oncoproteins, which showed that the PAMAM/5-FU conjugate
had a higher affinity for the oncoprotein than for 5-FU. Different generations of PAMAM dendrimers
(0.5G, 1.0G, 1.5G, 2.0G, and 2.5G) were synthesized, characterized and tested as drug carriers for 5-
FU. The PAMAM and PAMAM/5-FU drug conjugate showed less toxicity over COS-7 and HeLa cell
lines. Laser confocal imaging and western blotting for tumor suppressor proteins pRb and p53 were
used to confirm the interaction of PAMAM/5-FU with E6/E7 oncoproteins. Hematological analysis of
PAMAM/5-FU using BALB/c female mice with cervical cancer confirmed the less toxic nature of this
material. Based on these results, the developed PAMAM/5-FU conjugate is a potential candidate for
the treatment of cervical cancer.
Microbial community analysis in anaerobic palm oil mill effluent (pome) waste...eSAT Journals
Microorganisms play a key role in wastewater bio-treatment processes and understanding the microbial community structure is of great importance to improve treatment performance. Denaturing gradient gel electrophoresis (DGGE) was used to monitor succession of the microbial community and thus predominant bands were sequenced to reveal the microbial community composition inside palm oil mill effluent (POME) wastewater.DNA bands from DGGE gels were excised with a sterile blade and placed in 1.5 ml eppendorf tube containing 50 μl deionized water (ddH2O). Tubes were incubated overnight at 4C to elute the DNA. Eluted DNA was purified using QIAquick gel extraction kits (QIAGEN, Inc., Valencia, CA) and was frozen and thawed three times.Microbial DNA successfully excised and purified from DGGE was amplified using polymerase chain reaction (PCR).Five micro liters of the supernatant were used as a template to re-amplify the DNA using 16s rDNA primers,341f (with no GC-clamp) (5'- cct-acg-gga-ggc-agc-ag-3') and reverse(r) primers 907r (5'-ccc-cgt-caa-ttc-att-tga-gtt-t-3'). Amplification was repeated referring to the steps in ‘PCR amplification of 16s rDNA’. PCR products from agarose gels were cut and purified using QIAquick Gel Extraction Kit (QIAGEN, Inc., Valencia, CA), which were similar to the purification steps after recovery of DNA from DGGE, and sequenced in both directions with the same primers (with no GC-clamp) as used in PCR. Moreover, start-up is an important step in establishing proper community structure in all kinds of biological treatment processes. In anaerobic POME wastewater, 6 sequences of Firmicutes, 5 sequence of Proteobacterium and 2 sequences of Bacteroidetes were found through denaturing gradient gel electrophoresis (DGGE) results. Sequence closely related to Rummeliibacillus suwonensis strain G20 was detected grows at band BE10, BE11, BE12, BE15, BE16, BE17 and BE18. Meanwhile BE25 and BE26 were found at band after treatment process done.Rummeliibacillus suwonensis is an aerobic, Gram-positive, rod shaped, round-spore-forming bacteria which were isolated from aerobic condition. Keywords: Palm oil mill effluent (POME), anaerobic POME, Denaturing gradient gel electrophoresis (DGGE), and microbial community.
A primary screening of antitumor substances was carried out among strains of actinomycetes isolated from the samples of natural substrates of arid zones in the Ile-Balkhash region. Antitumor properties of actinomycetes against Staphylococcus aureus209Р(S. aureus 209P) and its mutants UF-2 and UF-3 were studied using the agar block technique. The diameter of growth inhibition zone was measured after incubation of the test microorganisms at a temperature of 37 °C for 24 hours. 16 strains of actinomycetes (10,2%) from sandy soils and from the plant rhizosphere of the Kapshagai area virtually having no effect or only slightly affecting the growth of wild-type culture.22 strains of actinomycetes (14,0%) from takyrs and takyr-like soils of the Balkhash area had an activity against staphylococcal mutants two or more times higher than against the stock staphylococcal strain.This strains may be the potential producers of antitumor antibiotics.It was established that 24.2% of the strains of actinomycetes may be potential producers of antitumor antibiotics.
Molecular Epidemiology entails the inclusion in epidemiological research of biologic measurement made at the molecular level-and thus is an extension of the increasing use of biologically based measures in epidemiological research (McMichael ,1994).The term ‘molecular epidemiology’ may suggest the existence of a sub-discipline with substantive new research content . Molecular techniques, however, are directed principally at enhancing the measurement of exposure, effect, or susceptibility , and not formulating new etiologic hypotheses. As techniques of refinement and elaboration , the integration of molecular measures into mainstream epidemiologic research can offer higher resolution answers in relation to disease causation.
26 ASBMB TODAY FEBRUARY 2021Discovering an old DoGs’ neMargaritoWhitt221
26 ASBMB TODAY FEBRUARY 2021
Discovering an old DoGs’
new trick
Heterotrimeric G proteins regulate
a variety of signaling pathways that
control cell development and influ-
ence cell morphology via actin/cyto-
skeleton remodeling. There are four
main families of G proteins: Gi/Go,
Gq, Gs and G12/13. Researchers long
have thought that Gs, unlike its family
members, is coupled specifically and
exclusively to adenylyl cyclases.
In a new study published in the
Journal of Biological Chemistry,
Alejandro Castillo–Kauil of the
Center for Research and Advanced
Studies of the National Polytechnic
Institute and collaborators challenge
this dogmatic view by identifying a
new Gs target. Using biochemical,
molecular biological and chemo-
genetic approaches, the researchers
demonstrated that the Gαs subfamily
of G proteins can regulate the activity
of Rho GTPases such as Rho guanine
nucleotide exchange factor, or Rho-
GEF. The interaction identified by the
group activates the small G protein
Cdc42 by Gs-coupled GPCRs, stimu-
lating a rearrangement of the cyto-
skeleton and inducing formation of
fingerlike protrusions called filopodia.
These results provide new insight
into G protein activity and define a
new role for RhoGEF coupling in G
protein function.
DOI: 10.1074/jbc.AC120.015204
A pathogen’s proteins target
mitochondria
The tick-borne pathogen Coxiella
burnetii causes Q fever, or query fever,
a rare flulike disease that can spread
to humans who inhale dust particles
contaminated by infected farm or
CONTINUED FROM PAGE 25
Noninvasive tool provides oral cancer prognosis
Oral squamous cell carcinoma, which affects about 34,000 people
in the U.S. each year, is found in the cells lining the lips and mouth.
Metastasis to the lymph nodes is a sign of disease progression and may
be accompanied by changes in proteolytic activity. During proteolysis,
enzymes cut up proteins into short fragments called peptides. Recent
work suggests that characterizing the sequence and abundance of these
molecules — a method dubbed peptidomics — might provide new in-
sight on cancer biology and in the clinic. In a recent paper in the journal
Molecular & Cellular Proteomics, Leandro Xavier Neves of the Brazil-
ian Biosciences National Laboratory and a team of Brazilian clinicians
and scientists describe their analysis of oral squamous cell carcinoma
patient saliva using peptidomics.
After extracting peptides from saliva samples, the research team ana-
lyzed and compared the peptide content in samples from patients with
and without metastasis to the lymph nodes. They found more than 1,000
uniquely expressed peptides in each group and an additional 1,628 pep-
tides expressed by both groups. A series of statistical analyses identified
77 peptides of particular interest; all of these peptides are overexpressed
in samples from patients with lymph node metastasis, which supports the
hypothesis that proteolytic activity increases ...
Applications of bioinformatics, main by kk sahuKAUSHAL SAHU
Introduction
Goals of Bioinformatics
Bioinformatics & Human Genome
Project
What can we do using bioinformatics ?
Applications of bioinformatics in various fields
1) Medicine
2) Evolutionary studies
3) Agriculture
4) Microbiology
5) Biotechnology
Conclusion
References
Pharmacokinetic Properties of Biomass-extracted Substances Isolated by Green ...Michal Jablonsky
According to the literature, approximately 41 nutraceutical compounds have been isolated from different types of biomass using green solvents. It is important to collect information on the pharmacokinetic properties of the nutraceutical substances from biomass isolated according to the published papers. The pharmacokinetic properties of the bioactive substances extracted by green solvents, such as the molecular weight, logP, AlogP, H-bond acceptor, H-bond donor, total polar surface area, atom molar refractivity, number of rotatable bonds, number of atoms, rotatable bond count, number of rigid bonds, number of atom rings, and number of H-bonds, were calculated with a drug-likeness tool. In practical terms, the original and most well-known Lipinski's Rule of Five (Ro5) was applied to 28 substances, namely 3-hydroxytyrosol; apigenin; artemisinin; bergapten; bilobalide; biochanin A; caffeic Acid; caffeoylmalic acid; catechins; cinnamic acid; curcumin; daidzei; daidzin; epicatechin; gallic acid; genistein; ginkgolide A; ginkgolide B; levofloxacin; luteolin; naringenin; p-coumaric acid; protocatechuic acid; psoralen; quercetin; trans-ferulic acid; tyrosol, and vanillin.
Study of Glutathione Peroxidase GPX Activity Among Betel Quid Chewers of Indi...ijtsrd
"Introduction Betel quid BQ chewing, a habit practiced in Eastern and North Eastern part of India, has known to be associated with cancer of the oral or buccal cavity. BQ is also one of the common mood elevating substances among Indian population. The BQ is a mixture of areca nut Areca catechu , catechu Acacia catechu and slaked lime calcium oxide and calcium hydroxide wrapped in a betel leaf Piper betel .BQ products have been classified by the International Agency for Research on Cancer IARC as group I human carcinogens . Glutathione peroxidise GPx , one of the major enzymatic antioxidant defence system, responsible for scavenging free radicals. Antioxidant enzymes catalyze decomposition of ROS. Overall balance between production and removal of ROS may be more important in various cancers including OSCC Oral squamous cell carcinoma or oral cancer. Methods In this study subjects were screened from Department of Oral and Maxillofacial surgery andE.N.T. of Ramakrishna Mission Seva Pratishthan Hospital RKMSP , Kolkata and different areas of West Bengal and North Eastern states of India. Quantitative in vitro determination of glutathione peroxidase activities in whole blood were estimated manually with 0.05 ml whole blood. The samples were assayed by UV Visible Spectrophotometer SPECORD 50 PLUS at a wavelength of 340nm. Results Most of the subjects had betel quid chewing habit. Glutathione peroxidase values are higher in healthy control than Cancer cases and Pre cancer with betel quid chewing habit, which is statistically significant. Conclusion Reactive oxygen species are generated due to slaked lime, one of the important constituents of betel quid which can modulate the oral pathology and promote carcinogenesis. Aniket Adhikari | Madhusnata De ""Study of Glutathione Peroxidase (GPX) Activity Among Betel Quid Chewers of Indian Population"" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3 , April 2019, URL: https://www.ijtsrd.com/papers/ijtsrd21619.pdf
Paper URL: https://www.ijtsrd.com/biological-science/biochemistry/21619/study-of-glutathione-peroxidase-gpx-activity-among-betel-quid-chewers-of-indian-population/aniket-adhikari"
PAMAM/5-fluorouracil drug conjugate for targeting E6 and E7 oncoproteins in c...Arun kumar
In the present study, poly(amidoamine)/5-fluorouracil (PAMAM/5-FU) was prepared and used as
a conjugate system for delivering drugs to target E6 and E7 oncoproteins, which are predominant in
cervical cancers. Specifically, molecular docking analysis was used to investigate the interaction
between the PAMAM/5-FU and E6/E7 oncoproteins, which showed that the PAMAM/5-FU conjugate
had a higher affinity for the oncoprotein than for 5-FU. Different generations of PAMAM dendrimers
(0.5G, 1.0G, 1.5G, 2.0G, and 2.5G) were synthesized, characterized and tested as drug carriers for 5-
FU. The PAMAM and PAMAM/5-FU drug conjugate showed less toxicity over COS-7 and HeLa cell
lines. Laser confocal imaging and western blotting for tumor suppressor proteins pRb and p53 were
used to confirm the interaction of PAMAM/5-FU with E6/E7 oncoproteins. Hematological analysis of
PAMAM/5-FU using BALB/c female mice with cervical cancer confirmed the less toxic nature of this
material. Based on these results, the developed PAMAM/5-FU conjugate is a potential candidate for
the treatment of cervical cancer.
Opendatabay - Open Data Marketplace.pptxOpendatabay
Opendatabay.com unlocks the power of data for everyone. Open Data Marketplace fosters a collaborative hub for data enthusiasts to explore, share, and contribute to a vast collection of datasets.
First ever open hub for data enthusiasts to collaborate and innovate. A platform to explore, share, and contribute to a vast collection of datasets. Through robust quality control and innovative technologies like blockchain verification, opendatabay ensures the authenticity and reliability of datasets, empowering users to make data-driven decisions with confidence. Leverage cutting-edge AI technologies to enhance the data exploration, analysis, and discovery experience.
From intelligent search and recommendations to automated data productisation and quotation, Opendatabay AI-driven features streamline the data workflow. Finding the data you need shouldn't be a complex. Opendatabay simplifies the data acquisition process with an intuitive interface and robust search tools. Effortlessly explore, discover, and access the data you need, allowing you to focus on extracting valuable insights. Opendatabay breaks new ground with a dedicated, AI-generated, synthetic datasets.
Leverage these privacy-preserving datasets for training and testing AI models without compromising sensitive information. Opendatabay prioritizes transparency by providing detailed metadata, provenance information, and usage guidelines for each dataset, ensuring users have a comprehensive understanding of the data they're working with. By leveraging a powerful combination of distributed ledger technology and rigorous third-party audits Opendatabay ensures the authenticity and reliability of every dataset. Security is at the core of Opendatabay. Marketplace implements stringent security measures, including encryption, access controls, and regular vulnerability assessments, to safeguard your data and protect your privacy.
As Europe's leading economic powerhouse and the fourth-largest hashtag#economy globally, Germany stands at the forefront of innovation and industrial might. Renowned for its precision engineering and high-tech sectors, Germany's economic structure is heavily supported by a robust service industry, accounting for approximately 68% of its GDP. This economic clout and strategic geopolitical stance position Germany as a focal point in the global cyber threat landscape.
In the face of escalating global tensions, particularly those emanating from geopolitical disputes with nations like hashtag#Russia and hashtag#China, hashtag#Germany has witnessed a significant uptick in targeted cyber operations. Our analysis indicates a marked increase in hashtag#cyberattack sophistication aimed at critical infrastructure and key industrial sectors. These attacks range from ransomware campaigns to hashtag#AdvancedPersistentThreats (hashtag#APTs), threatening national security and business integrity.
🔑 Key findings include:
🔍 Increased frequency and complexity of cyber threats.
🔍 Escalation of state-sponsored and criminally motivated cyber operations.
🔍 Active dark web exchanges of malicious tools and tactics.
Our comprehensive report delves into these challenges, using a blend of open-source and proprietary data collection techniques. By monitoring activity on critical networks and analyzing attack patterns, our team provides a detailed overview of the threats facing German entities.
This report aims to equip stakeholders across public and private sectors with the knowledge to enhance their defensive strategies, reduce exposure to cyber risks, and reinforce Germany's resilience against cyber threats.
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Empowering the Data Analytics Ecosystem: A Laser Focus on Value
The data analytics ecosystem thrives when every component functions at its peak, unlocking the true potential of data. Here's a laser focus on key areas for an empowered ecosystem:
1. Democratize Access, Not Data:
Granular Access Controls: Provide users with self-service tools tailored to their specific needs, preventing data overload and misuse.
Data Catalogs: Implement robust data catalogs for easy discovery and understanding of available data sources.
2. Foster Collaboration with Clear Roles:
Data Mesh Architecture: Break down data silos by creating a distributed data ownership model with clear ownership and responsibilities.
Collaborative Workspaces: Utilize interactive platforms where data scientists, analysts, and domain experts can work seamlessly together.
3. Leverage Advanced Analytics Strategically:
AI-powered Automation: Automate repetitive tasks like data cleaning and feature engineering, freeing up data talent for higher-level analysis.
Right-Tool Selection: Strategically choose the most effective advanced analytics techniques (e.g., AI, ML) based on specific business problems.
4. Prioritize Data Quality with Automation:
Automated Data Validation: Implement automated data quality checks to identify and rectify errors at the source, minimizing downstream issues.
Data Lineage Tracking: Track the flow of data throughout the ecosystem, ensuring transparency and facilitating root cause analysis for errors.
5. Cultivate a Data-Driven Mindset:
Metrics-Driven Performance Management: Align KPIs and performance metrics with data-driven insights to ensure actionable decision making.
Data Storytelling Workshops: Equip stakeholders with the skills to translate complex data findings into compelling narratives that drive action.
Benefits of a Precise Ecosystem:
Sharpened Focus: Precise access and clear roles ensure everyone works with the most relevant data, maximizing efficiency.
Actionable Insights: Strategic analytics and automated quality checks lead to more reliable and actionable data insights.
Continuous Improvement: Data-driven performance management fosters a culture of learning and continuous improvement.
Sustainable Growth: Empowered by data, organizations can make informed decisions to drive sustainable growth and innovation.
By focusing on these precise actions, organizations can create an empowered data analytics ecosystem that delivers real value by driving data-driven decisions and maximizing the return on their data investment.
1. GBRC NEWS
Volume III, Issue III
September 2023
KAUSHALYA TRAINING PROGRAM FOR SKILL DEVELOPMENT IN BIOTECHNOLOGY 2023-24
KAUSHALYA (Knowledge Advancement Using Skills on
High-end Applied LifetechnologY for Aspirants) is an
initiative by GBRC to help in developing
biotechnological skills of the researchers,
academicians and other stakeholders. The program's
aim is to provide learners extensive and specialized
practical knowledge for the development of their
functional skill set in biotechnology and related fields.
Total 12 trainings has been planned under the
program on the different advance tools and
technologies in the subject area of biotechnology.
UPCOMING TRAININGS
1
NETWORK PROGRAM ON AMR, SUPERBUGS AND ONE
HEALTH
2. CONTENTS
Page/2
Page/3
Page/7
Page/10
Page/11
GBRC in NEWS
GBRC IN NEWS
HONOURS
INVITED TALKS
MOUS
PRABODH
VISITS
RECENT PUBLICATIONS
Page/9
STUDENT STARTUP
INNOVATION GRANTS
CONFERENCES
STAFF WELFARE CLUB
ACTIVITIES
KAUSHALYA TRAINING
PROGRAMS
ARRIVAL & DEPARTURE
Page/12
Page/14
Page/20
Prof. Chaitanya Joshi, Director of GBRC has been conferred
with the prestigious SCROLL OF HONOR by SRBCE for his
outstanding contributions in the field at Navrachana
University, Vadodara
HONOURS
“The scientist is not a person who gives the right
answers, he’s one who asks the right questions.”
Claude Levi-Strauss
2
Page/21 NEW INSTRUMENTS
Page/8 STUDENT INNOVATION
FEST
3. Authors: Stuti N Patel, Ravi R Sonani, Mukesh G Chaubey, Gagan D Gupta, Niraj Kumar Singh, Vinay Kumar
& Datta Madamwar
Journal: 3 Biotech
Impact factor: 2.893
Phycobiliproteins is a family of chromophore-containing proteins having light-harvesting and antioxidant
capacity. The phycocyanin (PC) is a brilliant blue coloured phycobiliprotein, found in rod structure of
phycobilisome and has been widely studied for their therapeutic and fluorescent properties. In the present
study, the hexameric assembly structure of phycocyanin (Syn-PC) from Synechococcus Sp. R42DM is
characterized by X-ray crystallography to understand its light-harvesting and antioxidant properties. The
crystal structure of Syn-PC is solved with 2.15 Å resolution and crystallographic R-factors, Rwork/Rfree,
0.16/0.21. The hexamer of Syn-PC is formed by heterodimer of two polypeptide chains, namely, α- and β-
subunits. The structure is analysed at atomic level to reveal the chromophore microenvironment and
possible light energy transfer mechanism in Syn-PC. The chromophore arrangement in hexamer, deviation
angle and distance between the chromophore contribute to the energy transfer efficiency of protein. The
structural attributes responsible for the antioxidant potential of Syn-PC are recognized and annotated on its
3-dimensional structure.
CRYSTAL STRUCTURE OF SYNECHOCOCCUS PHYCOCYANIN: IMPLICATIONS OF LIGHT-HARVESTING
AND ANTIOXIDANT PROPERTIES
RECENT PUBLICATIONS
DEVELOPMENT OF EFFICIENT EMBRYO-DERIVED REGENERATION SYSTEM AND OPTIMIZATION OF
GENETIC TRANSFORMATION IN CUMIN (CUMINUM CYMINUM L.)
Authors: Komal K Sapara, Mansi Jani, Darshan Dharajiya, Fenil Patel, Amrut K Patel and Chaitanya G Joshi
Journal: Plant Cell, Tissue and Organ Culture
Impact factor: 2.7
Cumin is an important spice crop with high agronomic and economic importance. A direct regeneration
system using embryogenic explants in cumin (Cuminum cyminum L.) was established to develop a highly
efficient transformation system. Cumin embryos were utilized as an explant which shows higher
regeneration efficiency on Gamborg’s B5 media supplemented with 2.0 μM BA+ 0.5 μM NAA.
Transformation of pSIM24-eGFP plasmid in cumin was carried out through Agrobacterium tumefaciens EHA
105 and gene gun method. The transgenic explants were confirmed for GFP (green fluorescent protein)
gene integration through PCR analysis. The Agrobacterium-mediated transformed explants showed higher
regeneration and transformation efficiency with 0.5 OD600 of cell density and 24 hr of co-cultivation
compared to 0.4 OD600 with 24 hr, 48 hr, and 72 hr co-cultivation time and 0.5 OD600 with 48 hr and 72 hr
cocultivation time. It was further confirmed by GFP expression analysis through real-time PCR. Gene gun-
mediated transformed explants were cultured on different osmolytes (mannitol, sorbitol, and sucrose)
containing media to reduce bombardment stress on explants. Compared to mannitol and sucrose containing
media, transformed explants cultured on sorbitol-containing media showed higher rates of regeneration
and transformation.
These results were further confirmed by real-time
PCR analysis as prominent GFP expression was
found in explants cultured on sorbitol-containing
media compared to other osmolytes containing
media. In the current study, we have developed an
efficient transformation system with higher gene
expression and regeneration efficiency.
3
4. Authors: Urvi Budhbhatti, Ajay Chauhan, Deeptiben Bhatt, Chirag Parmar, Vishal Damani, Amrutlal Patel,
Chaitanya Joshi
Journal: Neuroscience Letters
Impact factor: 2.5
Authors: Manish Kumar, Madhvi Joshi, Guangming Jiang, Rintaro Yamada, Ryo Honda, Vaibhav Srivastava,
Jürgen Mahlknecht, Damia Barcelo, Sabarathinam Chidambram, Anwar Khursheed, David Graham,
Chaitanya Joshi
Journal: Environmental Pollution
Impact factor: 9.988
Wastewater-based epidemiology (WBE) monitoring can play a key role in managing future pandemics
because it covers both pre-symptomatic and asymptomatic cases, especially in densely populated areas with
limited community health care. In the present work, wastewater monitoring was employed in Ahmedabad,
India, after the successful containment of the first wave of COVID-19 to predict resurgence of the disease in
the expected second wave of the pandemic. Here we show wastewater levels of COVID-19 virus particles
(i.e., SARS-CoV-2) positively correlated with the number of confirmed clinical cases during the first wave
and provided early detection of COVID-19 presence before the second wave in Ahmedabad and an WBE-
based city zonation plan was developed for health protection. Monitoring upto eight month showed a
substantial decline COVID related gene markers between October and September 2020, followed by an
abrupt increase in November 2020. Similar changes were seen in March 2021, which preceded the second
COVID-19 wave. Measured wastewater ORF-1ab gene copies ranged from 6.1 × 102 (October, 2020) to 1.4 ×
104 (November, 2020) copies/mL, and wastewater gene levels typically lead confirmed cases by one to two
weeks. The study highlights the value of WBE as a monitoring tool to predict waves within a pandemic,
identifying local disease hotspots within a city and guiding rapid management interventions.
RESPONSE OF WASTEWATER-BASED EPIDEMIOLOGY PREDICTOR FOR THE SECOND WAVE OF
COVID-19 IN AHMEDABAD, INDIA: A LONG-TERM DATA PERSPECTIVE
RECENT PUBLICATIONS
ASSOCIATION OF NOTCH4 AND ACHE GENE POLYMORPHISM IN ALZHEIMER'S DISEASE OF GUJARAT
COHORT
Alzheimer’s Disease (AD) is the most common form of dementia, affecting cognitive and behavioral
functions. AD is a complex disease resulting from the modest effect of gene interaction and environmental
factors, as a result of which the exact pathogenesis is still unknown. The aim of the present study was to
investigate the association between variants of 98 targeted genes with Alzheimer’s disease phenotype. A
total of 98 genes from 32 AD cases and 11 controls were genotyped using the Haloplex target enrichment
method and the PCR-RFLP approach. Association analysis was performed using the PLINK tool to identify
the variant significantly associated with AD. Functional enrichment analysis and network analysis was
performed using ClueGo and String database respectively. The Expression Quantitative Trait Loci (eQTL)
analysis using the Genotype Tissue Expression (GTEx) dataset to explore the possible implication of the
variant on the expression of one or more genes in different brain regions and whole blood. Association
analysis showed significant association of 19 variant assigned to 16 genes with Alzheimer’s with p-value &
lt; 0.05 with rs367398/NOTCH4 only variant that passed multiple test corrections. Functional enrichment
analysis showed the association of these genes with AD. ClueGo and network analysis utilizing the String
database suggested that genes are directly and indirectly linked to the AD pathogenesis. eQTL analysis
revealed that the rs367398/NOTCH4 and rs1799806/ACHE variant showed significant eQTL for the
neighboring genes. The present study showed the possible role of 16 genes in AD pathogenesis, especially
highlighting the role of rs367398/NOTCH4 and rs1799806/ACHE. However further investigation with large
cohort is required to study and validate the implication of these variants in the AD pathogenesis.
4
5. Authors: Darshan T. Dharajiya, Nitin Shukla, Maharshi Pandya, Madhvi Joshi, Amrutlal K. Patel, Chaitanya G. Joshi
Journal: Frontiers in Plant Science
Impact factor: 5.6
RECENT PUBLICATIONS
RESISTANT CUMIN CULTIVAR, GC-4 COUNTERS FUSARIUM OXYSPORUM F. SP. CUMINI INFECTION
THROUGH UP-REGULATION OF STEROID BIOSYNTHESIS, LIMONENE AND PINENE DEGRADATION
AND BUTANOATE METABOLISM PATHWAYS
Cumin (Cuminum cyminum L.), an important spice crop belonging to the Apiaceae family is infected by Fusarium
oxysporum f. sp. cumini (Foc) to cause wilt disease, one of the most devastating diseases of cumin adversely
affects its production. As immune responses of cumin plants against the infection of Foc are not well studied,
this research aimed to identify the genes and pathways involved in responses of cumin (cv. GC-2, GC-3, GC-4,
and GC-5) to the wilt pathogen. Differential gene expression analysis revealed a total of 2048, 1576, 1987, and
1174 differentially expressed genes (DEGs) in GC-2, GC-3, GC-4, and GC-5, respectively. In the resistant cultivar
GC-4 (resistant against Foc), several important transcripts were identified. These included receptors,
transcription factors, reactive oxygen species (ROS) generating and scavenging enzymes, non-enzymatic
compounds, calcium ion (Ca2+) transporters and receptors, R-proteins, and PR-proteins. The expression of
these genes is believed to play crucial roles in conferring resistance against Foc. Gene ontology (GO) analysis of
the up-regulated DEGs showed significant enrichment of 19, 91, 227, and 55 biological processes in GC-2, GC-3,
GC-4, and GC-5, respectively. Notably, the resistant cultivar GC-4 exhibited enrichment in key GO terms such as
'secondary metabolic process', 'response to reactive oxygen species', 'phenylpropanoid metabolic process', and
'hormone-mediated signaling pathway'. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG)
pathway analysis revealed the enrichment of 28, 57, 65, and 30 pathways in GC-2, GC-3, GC-4, and GC-5,
respectively, focusing on the up-regulated DEGs. The cultivar GC-4 showed enrichment in pathways related to
steroid biosynthesis, starch and sucrose metabolism, fatty acid biosynthesis, butanoate metabolism, limonene
and pinene degradation, and carotenoid biosynthesis. The activation or up-regulation of various genes and
pathways associated with stress resistance demonstrated that the resistant cultivar GC-4 displayed enhanced
defense mechanisms against Foc. These findings provide valuable insights into the defense responses of cumin
that could contribute to the development of cumin cultivars with improved resistance against Foc.
5
6. Authors: Arpit Arunkumar Bana, Nithin Sajeev, Sabyasachi Halder, Haidar Abbas Masi, Shikha Patel, Priti
Mehta
Journal: Heliyon
Impact factor: 4.0
Bevacizumab (Bvz) is the most preferred recombinant humanized monoclonal antibody in bio-similar
development due to its prominence as a standard treatment in the oncology space. Therapeutic monoclonal
antibodies are typically more complex and unlikely to produce a replica. As a result, regulatory agencies
allow approval of biosimilars that differ structurally and functionally from their reference product, but
these differences should not have any clinical significance. To identify these significant discrepancies, it is
essential to perform a thorough characterization of critical product attributes both in real-time and after
storage until the product’s expiration. In the present study, two Bvz biosimilar brands (Bio-1 and Bio-2)
marketed in India were evaluated and compared with the reference product Avastin® to assess their degree
of similarity. A comprehensive physicochemical characterization of biosimilars and reference product was
performed using orthogonal techniques including LC-ESI-QTOF, MALDI-TOF, FTIR-ATR, iCIEF, rCE, nrCE,
UV280, and RP-HPLC. Furthermore, Bvz formulations under study were subjected to various stress
conditions of thermal (elevated temperature 50 ± 2 ◦C), chemical (acidic pH 3.0 ± 0.2, neutral pH 7.0 ± 0.2,
and basic pH 10.0 ± 0.2), and mechanical (agitation 200 rpm) for comparative stability evaluation. Any
alteration in the secondary structure of the native protein was detected and quantified using far-UV circular
dichroism (CD), indicating an average of 15% and 11% loss in native antiparallel β-sheet conformation
respectively in Bio-1 and Bio-2 upon exposure to elevated temperature and high pH. Additionally, covalent
or non-covalent aggregates formed as a function of elevated temperature and agitation were quantified
using SEC-MALS.
COMPARATIVE STABILITY STUDY AND AGGREGATE ANALYSIS OF BEVACIZUMAB MARKETED
FORMULATIONS USING ADVANCED ANALYTICAL TECHNIQUES
RECENT PUBLICATIONS
A SECOND UPDATE ON MAPPING THE HUMAN GENETIC ARCHITECTURE OF COVID-19
Authors: Madhvi N. Joshi, Raghav D. Dixit, Pranay K. Shah, Kamlesh J. Upadhyay, Naresh T. Chuhan, Kairavi J.
Desai, Meenakshi R. Shah & Bhavesh Modi, Chaitanya G. Joshi, Ramesh J. Pandit, Indra Singh, Afzal I. Ansari,
Janvi N. Raval, Zarna Z. Patel
Journal: Nature
Impact factor: 69.504
Investigating the role of host genetic factors in COVID-19 severity and susceptibility can inform our
understanding of the underlying biological mechanisms that influence adverse outcomes and drug
development. Here we present a second updated genome-wide association study (GWAS) on COVID-19
severity and infection susceptibility to SARS-CoV-2 from the COVID-19 Host Genetic Initiative (data release
7). We performed a meta-analysis of up to 219,692 cases and over 3 million controls, identifying 51 distinct
genome-wide significant loci adding 28 loci from the previous data release. The increased number of
candidate genes at the identified loci helped to map three major biological pathways that are involved in
susceptibility and severity: viral entry, airway defence in mucus and type I interferon. We conducted a meta-
analysis for 3 phenotypes across 82 studies from 35 countries, including 36 studies of individuals with non-
European ancestry (Fig. 1, Supplementary Figs. 1 and 2 and Supplementary Table 1): critical illness
(respiratory support or death; 21,194 cases), hospitalization (49,033 cases) and SARS-CoV-2 infection
(219,692 cases). Most of the studies were collected before the widespread introduction of COVID-19
vaccination. We found 30, 40 and 21 loci that are associated with critical illness, hospitalization and
infection due to SARS-CoV-2, respectively, for a total of 51 distinct genome-wide significant loci across all
three phenotypes.
6
7. INVITED TALKS DELIVERED BY GBRC TEAM
• Dr. Niraj Kumar Singh, Joint Director, GBRC delivered lecture on “Preparation for Competitive CSIR-NET/GATE
exams” at NIRMA University, Ahmedabad on 20th July, 2023.
• Dr. Amrutlal K. Patel, Joint Director, GBRC delivered lecture on “Gene delivery system: approaches for the
treatment of hereditary disorders and cancer” at Arihant School of Pharmacy & Bio Research Institute,
Gandhinagar on 21st August, 2023.
• Dr. Neeraj Kumar Singh, Joint Director, GBRC delivered lecture on “Novel alpha Amylase from hotspring
metagenome” at Unveiling Innovation IKA new product launch, Chandigarh on 25th August, 2023.
• Dr. Madhvi Joshi, Joint Director, GBRC attended panel discussion on “Wastewater surveillance and other
applications of molecular technologies in surveillance” in a workshop on “Building Back Better Surveillance
Systems” co-hosted by the Tata Institute for Genetics & Society and the Global Learning Collaborative for Health
Systems Resilience in Bengaluru on 31st August-1st September 2023.
• Dr. Madhvi Joshi, Joint Director, GBRC was invited as the chief guest at the inauguration of the Gujarat State
Biotechnology Mission - sponsored Bioinformatics training program at Samarpan Science and Commerce College,
Gandhinagar on 25th September, 2023
MOU’S
• GBRC signed MoU with Nirma
University on 20th July, 2023. The aim
is to pursue joint research addressing
societal problems and issue prevalent
in Gujarat state in the area of
Biotechnology.
• GBRC signed MoU with Tata Institute for
Genetics and Society, Bengaluru on 31st
August, 2023. The aim is to pursue joint
research addressing societal problems
and issue prevalent in the field of
Biotechnology.
• GBRC signed MoU with Swaminarayan
University, Kalol on 1st September, 2023.
The aim is to pursue joint research
addressing societal problems and issues
prevalent in Gujarat state in
Biotechnology.
7
8. STUDENTS INNOVATION FEST
• As a part of Student Innovation Fest -2023 (Third Edition) organized by Vigyan Gurjari Gujarat Prant, a state unit
of Vijnana Bharati (VIBHA), GBRC staff delivered lectures in various school/institute/university. This activity was
conferred with World Records India Award as longest lecture series.
• Dr. Ishan Raval, Scientist-B, GBRC delivered a lecture on “Introduction to mass spectroscopy and proteomics” at
Gandhinagar University, Gandhinagar on 10th August, 2023.
• Dr. Satyamitra Shekh, Scientist-B, GBRC, delivered a lecture on "Probiotics and Prebiotics" at Ambajipura Primary
School, Santej, Ahmedabad on 10th August, 2023.
• Dr. Sanman Samova, Scientist-B, GBRC, delivered a lecture on “Unleashing the Power of Biotechnology: From Lab
to Life” at M. M. Vasa Primary and High School, Koba, Gandhinagar on 10th August, 2023.
• Dr. Haidar Abbas, Scientist-B, GBRC, delivered a lecture on "Separation Techniques and Applications" at L.J.
Institute of Engineering & Technology (L.J.I.E.T), Ahmedabad on 10th August, 2023.
• Mr. Narottam Jepal, AAO, GBRC, delivered a lecture on “Innovation and Entrepreneurship” at Silver Oak
University, Ahmedabad on 10th August, 2023.
8
9. STUDENT STARTUP INNOVATION GRANTS
Gujarat Technological University Innovation & Start-up Centre Conveys and allocate a grant to innovator for the
following project under the selection for the grant scheme of Student Startup Innovation Policy 2.0 on 2nd August,
2023. Following belong to GBRC are honoured with grant for their startups.
Prof. Chaitanya Joshi, Director, GBRC attended Vigyan Bharti Conference at Lok Vigyan Kendra, Dahod on
10th August, 2023.
Dr. Bhumika Prajapati
Nalfia device for Dengue detection
Dr. Pooja Doshi
NaHotech Bioscience
Purva Patel
Swasth Flora
CONFERENCES
Dr. Madhvi Joshi, Joint Director, GBRC at panel discussion on “Wastewater surveillance and other
applications of molecular technologies in surveillance” in a workshop on “Building Back Better Surveillance
Systems” co-hosted by the Tata Institute for Genetics & Society and the Global Learning Collaborative for
Health Systems Resilience at Bengaluru on 31st August-1st September, 2023.
9
10. PRABODH
GBRC is conducting "PRABODH" (Promoting Research Awareness in Biotechnology for Development of Human
Resource) to accelerate the research work and to develop research-oriented thought processes in staff.
INVITED GUESTS
Expert (15/07/2023)
Dr. Nitesh Kumar Mistry
Professor and Head, Department of
Microbiology, Arts, Science and R.A. Patel
Commerce College, Borsad
Topic: Entrepreneurship opportunities in biotechnology.
JULY-2023
Expert (15/07/2023)
Shri Parth Shastri
Senior Assistant Editor,
The Times of India (TOI), Ahmedabad
Topic: The role of media and how media highlights scientific
research for the larger audience.
Article: Baculovirus surface
display of Zika virus envelope
protein protects against virus
challenge in mouse model
Journal Name: Virologica
Sinica
Impact Factor: 6.947
Dr. Sanman Samova
Scientist-B
Ms. Mansi Jani
JRF
Article: Transcription
activator-like effectors protects
bacterial endosymbionts from
entrapment within fungal
hyphae
Journal Name: Current
Biology
Impact Factor: 10.9
PRESENTATIONS FROM GBRC MEMBERS
Dr. Vasan Sambandamurthy,
Senior Vice President - Global Operations,
Bugworks™ Research
INVITED GUEST
AUGUST-2023
Topic: Challenges in developing a novel broad-spectrum antibacterial agent. 10
11. PRESENTATIONS FROM GBRC MEMBERS
Article: Derepression of
specific miRNA-target genes
in rice using CRISPR/Cas9
Journal: Journal of
Experiment Botany
Impact factor: 7.378
Dr. Hemanshu Maisuria
TA, GBRC
Ms. Monica Chavan,
JRF, GBRC
Article: Nonnutritive
sweeteners can promote the
dissemination of antibiotic
resistance through
conjugative gene transfer
Journal: International
Society for Microbial Ecology
Impact factor: 11.217
INVITED GUEST
SEPTEMBER-2023
Prof. Ashok Pandey,
Distinguished Scientist, CSIR-Indian Institute
of Toxicology Research,
Lucknow
Topic: Microplastics/ nanoplastics pollution and abatement for
sustainable development
PRESENTATIONS FROM GBRC MEMBERS
Article: Commensal Candida albicans positively calibrates systemic Th17
immunological responses
Journal: Cell Host & Microbe
Impact factor: 31.316
GBRC wishes best for the future of the bright
minds who had left
ARRIVAL & DEPARTURE
GBRC would like to extend a hearty
Welcome of the new members to family
Dr. Hemangkumar Brahmabhatt
Dr. Sonali Thakur
Dr. Vijay Nimkande
Ms. Jayvi Patel
Mr. Aveeral Chaudhary
Mr. Sandip Kanjibhai Chaudhary
Mr. Anubhav Tamrakar
Dr. Dishant Patel
Dr. Kartikkumar Gajjar
Dr. Anupam Kumari
Mr. Hardik Patni
Ms. Bhavika Parekh
Mr. Fenil Parmar
Mr. Abhijeeth Nair
Mr. Raj Gajjar
Mr. Manish Gupta
Dr. Nisarg Gohil
Ms. Suchi Patel
Mr. Nikhil Mehra
Ms. Bhavya Nanavati
Dr. Amisha Kushwaha
Ms. Trusha Baraiya
Ms. Deepika Panda
Dr. Maitri Trivedi
Mr. Rupesh Thorat
Dr. Himanshu Joshi
Dr. Damyanti Prajapati
Dr. Sonali Thakur
Dr. Dishant Patel
Mr. Animesh Singh
Ms. Jayvi Patel
Mr. Kartik Deopujari
Mr. Abhijeeth M Nair
11
12. STAFF WELFARE CLUB ACTIVITIES
The main objective of the Staff Welfare Club is to establish, promote, subsidize, encourage, provide, maintain,
organize, undertake, manage, equip, develop, recondition, operate, conduct and to run for in the music, dance, sports,
social welfare, carry out scientific and technical, other than political activities.
BEST MONTHLY
PRESENTATION AWARD
AWARD FOR BEST QUESTION
IN PRABODH
EMPLOYEE OF THE MONTH
AWARD
Ms. Shreya Johnson
(JRF)
Mr. Gufran Siddiqui
(JRF)
Mr. Vikas Patidar
(TA)
BEST CUBICLE AWARD
Anaerobic Laboratory
BEST MONTHLY
PRESENTATION AWARD
EMPLOYEE OF THE MONTH
AWARD
Mr. Priyank Chavda
(TA)
Dr. Himanshu Joshi
(RA)
BEST CUBICLE AWARD
Proteomics Laboratory
JULY-2023
AUGUST-2023
12
13. is
STAFF WELFARE CLUB ACTIVITIES
BEST MONTHLY
PRESENTATION AWARD
BEST MONTHLY
PRESENTATION AWARD
EMPLOYEE OF THE MONTH
AWARD
Mr. Aman Tripathi (JRF) Ms. Mansi Jani (JRF) Ms. Shreya Johnson (JRF)
BEST CUBICLE AWARD
Animal Tissue Culture Laboratory
SEPTEMBER-2023
MONTHLY EVALUATION ACTIVITY
GBRC has internal evaluation system of the project progress where all the fellows present their work for the month
and their performances are also evaluated in front of external expert.
Dr. Swati Joshi,
Scientist B,
ICMR –NIOH
Ahmedabad
Dr. Gunjan Sharma
Assistant Professor,
Gujarat Biotechnology University,
Gandhinagar
Dr. Sanjay Kumar
Assistant Professor,
National Forensic Science University,
Gandhinagar
September-2023
August-2023
July-2023
LIGHTER NOTES
13
▪ DNA can be used to store data just like your typical hard drives.
Scientists have been able to store 700 terabytes in a single gram of
DNA.
14. VISIT BY DIGNITARIES
Faculty members from Institute of Science, Silver Oak University, Ahmedabad
Prof. (Dr.) Col. A. K. Gahlot, Advisor to Governor, Rajasthan & Ex VC, Rajasthan University of Veterinary and Animal
Sciences, Bikaner
Mr. Czek Haan Tan, General Manager, Asia Pacific Region and Mr. Arun Natrajan, Principal Engineer from GE Additive
14
15. VISIT BY DIGNITARIES
Shri Surendra Patel, President of CHARUSAT University
Shri P. M. Jadeja, MLA, Gujarat
Shri S. B. Dangayach, Founder Trustee, Innovative Thought Forum
15
16. VISIT BY COLLEGE/ ACADEMIC INSTITUTES
Students and faculty members from Parul Institute of Applied Sciences, Parul University, Vadodara
Students and faculty members from Arihant School of Pharmacy & Bio-Research Institute, Gandhinagar
Students and faculty members from School of Science and Technology, Vanita Vishram Women's University, Surat
16
17. VISIT BY COLLEGE/ ACADEMIC INSTITUTES
Students and faculty members from B N Patel Institute of Paramedical and Science, Anand
Students and faculty members from School of Science, Indrashil University , Ahmedabad
Students and faculty members from School of Medico-Legal Studies, National Forensic Sciences University, Gandhinagar
17
18. VISIT BY COLLEGE/ ACADEMIC INSTITUTES
Students and faculty members from Navjivan Science College, Dahod
Students and faculty members from Pragati School, Ahmedabad
Students and faculty members from School of Pharmacy, Dr. Subhash University, Junagadh
18
19. VISIT BY COLLEGE/ ACADEMIC INSTITUTES
Students and faculty members from Gujarat Cancer & Research Institute (GCRI), Ahmedabad
Students and faculty members from Parul Institute of Applied Sciences and Research, Parul University, Ahmedabad
Students and faculty members from Institute of Science, Gandhinagar University, Gandhinagar
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20. VISIT BY COLLEGE/ ACADEMIC INSTITUTES
Students and faculty members from Department of Biomedical Engineering, Institute of Technology, Ganpat University,
Mehsana
KAUSHALYA TRAINING PROGRAMS
COMPLETED TRAININGS
UPCOMING TRAININGS
No Training Date
1 Plant Tissue Culture and Transgenics 9th – 21st October 2023
2 Analytical Techniques: Isolation to Identification 20th – 1st November 2023
3 In vitro Fertilization 11th – 22nd December 2023
No Training Date
1 Capillary Sequencing and Fragment Analysis 10th – 21st July 2023
2 Next Generation Sequencing 14th – 25th August 2023
3 Metagenomic Data Analysis 11th – 22nd September 2023
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21. CONFERENCE
Contact Information
Gujarat Biotechnology Research Centre (GBRC)
Department of Science & Technology,
Government of Gujarat,
6th floor, M. S. Building, Sector 11,
Gandhinagar, Gujarat, 382011, India.
Email id: info-gbrc@gujarat.gov.in
Contact no.: +91-079- 23258500
Website: https://gbrc.gujarat.gov.in; http://gbrc.res.in
For Shared Lab Facility : https://gbrc.org.in/
Editor in Chief:
Prof. Chaitanya G. Joshi
Director - GBRC
Executive Editors:
Editorial Team:
Dr. Sonal Sharma (Scientist B- GBRC)
Dr. Sanman Samova (Scientist B- GBRC)
Nimesh Patel (Technical Assistant- GBRC)
Dr. Madhvi Joshi
Joint-Director – GBRC
Dr. Amrutlal Patel
Joint-Director – GBRC
LabChip GX Touch HT Nucleic Acid Analyzer
NEW INSTRUMENT RECRUITMENT
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