This document provides a summary of Fengkun Du's career and qualifications. It outlines his experience as a microbiologist with over 7 years working in proteomic research and the wastewater industry. It also details his education including a PhD in Applied and Environmental Microbiology from Georgia State University and collaborations with various universities. His skills include protein purification techniques, cell culture, fermentation, microarray data analysis and more.

1. FengkunDu
70128 4th St, Covington, LA 70433
Cell: 678-451-8294, Email: supstrive@hotmail.com
CAREER SUMMARY
 Accomplished microbiologist specialized in protein purification, gas chromatography, cell
culture and fermentation with more than seven years of experience in microbiology and
proteomic research. Our laboratory collaborated with CDC, 3M, etc.
 Wastewater industry experience in developing innovative procedures and projects (research
and development), conducting respirometry/treatability research and microbiology analyses,
and preparation of client reports. Our clients include International Paper, Georgia-Pacific,
Bayer CropScience, Arcadis, Marathon, etc.
 Strong knowledge of microarray data analysis software including IPA, GeneSifter and MeV.
 Remain up to date with the development and advances in the industry through the collaboration
with Xavier University in Louisiana on cancer research.
HIGHLIGHTS
 Fast protein liquid chromatography (Äkta
Explorer 100, Unicorn 5.11)
 Protein quantification using BCA protein
assay, 2D Quant Kit protein assay and
Bradford protein assay
 Gradient SDS PAGE, gradient blue native
PAGE, and blue native-SDS two
dimensional gel (18×16cm plate)
 Gel imaging and analysis (Typhoon
Imager 9400, Image Quant TL)
 Microbial techniques including plating,
microbial identification & isolation
(biochemical tests), and anaerobic
microbial cultivation
 Gas Chromatography (Perkin Elmer
Autosystem XL, Totalchrom)
 Fermentation (10, 20 and 40 liters)
 Microarray data analyses using IPA, MeV
9.0, and GeneSifter softwares
 Western blot
 Cell culture
 Enzyme Assays/Endotoxin Assay
 Exocellular polysaccharide extraction
 Live/dead fluorescent staining
 E. coli, and total/fecal coliform
quantification
 Biotrickling filter reactor, Challenge
respirometer, RSA respirometer,
Eckenfelder reactor
 Zeiss CLSM 510 (Confocal Laser
Scanning Microscope)
 Millipore EasyCyte flow cytometer
PROFESSIONAL EXPERIENCE
May 2013 to Present
Environmental Business Specialists (EBS), Mandeville, LA 70448
Staff Scientist
 Fulfill microbiological analyses and respirometry studies on client wastewater samples.
 Compose and review client reports in a timely manner in deadline driven environments.
 Provide technical support and advice to chemistry and microbiology laboratory management
team and personnel regarding test procedures, data quality, results interpretation, and
troubleshooting.
 Lead the conceptualization and development of new biological products and processes that
are marketed to EBS clients and prospects.

2. FengkunDu
 Cooperate with field support and project personnel to ensure thorough understanding and
application of EBS products and services, project completion and client satisfaction.
 Perform on site analysis for client compliance and troubleshooting.
 Clients include International Paper, Georgia Pacific, Bayer CropScience, Arcadis, Marathon,
WestRock, Domtar, PCA, etc.
September 2013-present
Xavier University of Louisiana, New Orleans, LA 70125
Collaborating with Dr. Qian-Jin Zhang on cancer immunology (ovarian cancer) projects
 Compare the gene transcription differences between highly aggressive and low aggressive
ovarian cancer cell lines.
 Analyze microarray data and built potential molecular network.
October 2014-Jan 2015
Tulane University, New Orleans, LA 70118
Part-time Staff
 Cell culture, plasmid extraction and animal experiment.
August 2006 to May 2013
Georgia State University, Atlanta, GA 30303
Ph.D. student of the Applied and Environmental Microbiology (AEM) Program
Graduate Research Assistant:
 Conducted thesis work on comparison of the activity of an industrial enzyme (nitrile
hydratase) from two different bacterial strains with eight different growth conditions, and
purification and characterization of this industrial enzyme from the two strains.
 Collaborated with industrial/scientific clients to direct the purification of an influenza
vaccine from an E. coli recombinant strain.
 Performed bioremediation of acrylonitrile and toluene using a bench-top trickling filter.
 Identified proteins related to nylon 6,6 biodegradation from a bacterial strain.
EDUCATION
August 2006 – May 2013
Georgia State University, Atlanta, GA
Ph.D. of Science in Applied and Environmental Microbiology. GPA: 3.95
August 2005 – July 2006
Lanzhou University, Lanzhou, Gansu, China (Transferred to Georgia State University)
August 2001 – July 2005
Lanzhou University, Lanzhou, Gansu, China
Bachelors of Science in Biology.
PUBLICATIONS AND CONFERENCES
The Biomass Surface Properties in Full-Scale Activated Sludge Systems. Fengkun Du,
Elizabeth Dillman, Andrew J. Englande, Jr., Clifford R. Lange, Linxiong Li, Jiaqing Xu, William
Martin, Richard Dolly, Nathan Hathaway, Linda Rogers, Michael Foster. (Manuscript)

3. FengkunDu
Highly and moderately aggressive mouse ovarian cancer cell lines exhibit differential gene
expression. Fengkun Du; Yan Li; Shubha P. Kale; Harris McFerrin; Guangdi Wang; Elena
Skripnikova; Thomas Wiese; Xiao-Lin Li; Yong-Yu Liu; and Qian-Jin Zhang. (Tumor Biology,
2016)
Limited density of an antigen presented by RMA-S cells requires B7-1/CD28 signaling to
enhance T-cell immunity at the effector phase. Xiao-Lin Li, Marjolein Sluijter, Elien M.
Doorduijn, Shubha P. Kale, Harris McFerrin, Yong-Yu Liu, Yan Li, Madhusoodanan Mottamal,
Xin Yao, Fengkun Du, Baihan Gu, Kim Hoang, Yen H. Nguyen, Nichelle Taylor, Chelsea R.
Stephens, Thorbald van Hall and Qian-Jin Zhang. PLOS ONE. (November, 2014).
Highly and Moderately Aggressive Mouse Ovarian Cancer Cell Lines Exhibit Differential Gene
Expression. Fengkun Du; Yan Li; Shubha P. Kale; Harris McFerrin; Guangdi Wang; Elena
Skripnikova; Thomas Wiese; Xiao-Lin Li; Lan Davenport; Yong-Yu Liu; and Qian-Jin Zhang.
The Louisiana Cancer Research Center (LCRC). (March, 2014).
Comparison of Nitrile Hydratases in Rhodococcus rhodochrous DAP 96253 and DAP 96622
Growing on Inducing and Non-Inducing Media. Fengkun Du. Biology Dissertations. (2013).
Paper 130.
Comparison of Nitrile Hydratases in Rhodococcus rhodochrous DAP 96253 and DAP 96622
Growing on Inducing and Non-Inducing Media. Fengkun Du, Oscar Galagarza, Yaoyao Liu,
Trudy A. Tucker and George E. Pierce. SIMB Annual Meeting & Exhibition. (2012).
Comparison of nitrile hydratase in Rhodococcus rhodochrous DAP 96253 when induced with
different metals with/without urea. Fengkun Du, Guenevere Perry and George E. Pierce. SIM
Annual Meeting and Exhibition. (2011).
Volunteer Work
September 2013-May 2014, August 2015-December 2015
The Academy of Chinese Studies in New Orleans, New Orleans, LA 70118
Volunteer position
 Chinese teacher for students 5-13 years old.
 Teacher assistant for Chinese art class for students 5-13 years old.
 Additional work directed by the school president.
AWARDS AND FELLOWSHIPS
2013, Danold Ahearn Fellowship
2012, Outstanding Student Poster Presentation (Environmental Microbiology), Society for
Industrial Microbiology and Biotechnology (Annual Meeting)
2012, Graduate Award for Outstanding Instruction
2011-2013, Molecular Basis of Disease Graduate Fellowship

4. FengkunDu
REFERENCES:
References will be provided upon request.