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Page 1
Nematode biology
Page 2
Content
• Introduction
• Appearance and structure
• Life cycle
• Nematode types
Migratory endoparasites
Sedentary endoparasites
Ectoparasites
• Nematode shapes
Page 3
Introduction
• Nematodes are a diverse group of worm-like animals.
• They live virtually in every environment, both as parasites
and as free-living organisms.
• They are minute generally thus visible only under
microscope.
• Nematodes cause significant damage on crops leading to
huge yield losses.
Page 4
Appearance and structure
• Plant parasitic nematodes are thread-like worms ranging
from 0.25mm to >1.0mm long through some grow up to
4.0mm.
• Nematodes vary in shape and sizes through most are
known to taper towards the head and tail.
• Females of some species are known to lose the worm-like
shape as they mature and become enlarged taking a
pear, lemon or kidney shape or spherical as adults.
• Nematodes have circulatory, respiratory and digestive
system like all animals.
Page 5
Appearance and structure
• Plant parasitic nematodes differ
from nematodes which feed on
bacteria and fungi in that they
have a specialized feeding
structure, a spear or stylet.
• They use this stylet to inject
enzymes into plant cells and tissue
and then extract content in a
similar way that aphids feed on
plant.
Page 6
Appearance and structure
• The stylet is connected to the pharynx that, in turn, is
connected to the intestine.
• The intestine ends at the rectum in the female nematode
and the cloaca in the male.
• Attached to the pharynx are three - five salivary glands
which produce secretions that may be emitted from the
stylet and that assist the nematode in plant invasion and
parasitism.
Page 7
Appearance and structure
• The pharynx is a muscular specialize area that can
contact and expand the esophageal lining.
• The expansions and contraction of the pharynx muscles
allow the nematode to pump food into the intestine
through its stylet or eject secretion fro its salivary glands
into and around plant cells.
Page 8
Appearance and structure
• They have an outer skin or cuticle that is secreted from an
inner hypodermis.
• The muscles are attached longitudinally to the
nematode’s hypodermis, allowing them to move only in
the dorsal ventral direction (snake-like movement).
• Inside the nematode there is an inner tube, the alimentary
canal, which runs inside the nematode from head to tail.
Page 9
Appearance and structure
• Between the alimentary canal and the body wall is fluid
that provides pressure against the wall to maintain body
shape and allow movement.
• In the middle to posterior of the nematode are the
reproductive organs (vulva and spicule).
• Nematode species often have both males and females,
but it is not uncommon for plant nematodes to reproduce
asexually by parthenogenesis.
Page 10
Appearance and structure
• The number of ovaries and position of vulva in female
vary.
• Male nematodes have one or two testes and they are
easily identified by the presence of spicules.
Page 11
Appearance and structure
• Nematodes are triploblastic,
containing three body layers
(ectoderm, mesoderm and
endoderm) in the embryo.
• Nematodes have a body
cavity that is not totally
surrounded by mesoderm, so
they are pseudocoelomic.
Page 12
Structure and types of stylets
• Nematodes have different stylets for which they use to
obtain nutrients from their food sources.
• There are two types of stylets namely:
Stomato stylet- This is divided into 3 the knobs, shaft and
cone plus a guiding ring in between the shaft and cone.
Odonto stylet – This has also the 3 parts knob shaft and
cone.
Page 13
Stomato stylet
The shaft and cone are
separated by a guiding ring
and restricts fluid movement
from the nematode to the host
the nematode feeds on. The
knobs are larger compared to
those of odonto stylet.
E.g. Tylenchulus
Page 14
Odonto stylet
• These stylets lack the guiding
ring between the shaft and
cone and thus the nematode
fluids are able to flow into the
target host and thus able to
transmit diseases e.g. plant
viruses
• E.g. Xiphinema
Page 15
Life cycle
• A nematode life cycle
is divided into 6
stages namely: the
egg, 4 juvenile stages
and adult.
• Duration of the stages
differ in different
species and also
depending on factors
such as temperature,
moisture and plant
host.
Page 16
Cont..
• Nematodes are evolutionarily related to insects, and one
feature they have in common is the requirement to molt
between juvenile stages.
• All nematodes undergo four molts from the juvenile to the
adult phase of their life cycle.
• They have four juvenile stages and an adult stage.
• In many nematodes the first molt usually occurs in the
egg and it is the second-stage juvenile that hatches.
Page 17
Life cycle
• Under favourable conditions in the tropics many species
have relatively short life cycles.
• This leads to several generations per each season thus
rapid population build up.
• Nematodes are known to survive unfavorable conditions
such as dry season or cold winter in cyst form.
• Different species are known to survive best at different
stages e.g. Heterodera species survive best as egg
encapsulated with cyst, Anguina species as second stage
juvenile etc.
Page 18
Nematode types
• Nematodes can be separated into 2:
 Aerial parasites i.e those which feed on above ground
parts of plant.
Root and tuber parasites i.e those which feed on below
ground plant parts.
• Nematodes can also be grouped by their feeding behavior
and motility as follows:
Migratory endoparasitic nematodes
Sedentary endoparasites nematodes
Ectoparasitic nematodes
Page 19
Ectoparasites
• Ectoparasites feed from root tissue by inserting their stylet
from outside the root .
• The group consists of several morphologically divergent
families that have evolved different feeding strategies.
• As a rule, species that have a short stylet feed on
epidermal cells e.g. Tylenchorhychus dubius and those
with long stylets feed on deeper tissues e.g.
Belonolaimus, and Dolichodorus spp. Ectoparasites are
either migratory or sedentary.
Page 20
Ectoparasitic nematodes
• These nematodes feed on the
plant from the outside of the
plant.
• They feed externally on surface
of the plant usually on root hairs
or cortical tissue.
• Often found in high densities
though do not pose a problem.
• They however cause serious
damage if the plant is suffering
abiotic or biotic stresses such as
fungal attach or low water
availability.
Page 21
Ectoparasitic nematodes
• Examples of ectoparasitic nematodes
include:
• Criconemoides spp. (ring nematodes)
• Helicotylenchus spp.( spiral
nematodes)
• Aphelenchoides besseyi ( aerial rice
white-tip nematodes)
• Xiphinema spp.(dagger nematodes)
• Longidorus spp. (needle nematodes)
• Trichodorus & Paratrichodorus spp.
(stunt nematodes).
Trichodorus spp
Criconemoides spp
Page 22
Ectoparasitic nematodes
• It has been found that some ectoparasitic nematodes
transmit plant virus e.g. Longidorus spp, Xiphinema spp,
Trichodorus spp and Paratrichodorus spp.
Page 23
Migratory ectoparasites
• These nematodes have the most primitive mode of
parasitism; they remain outside of the root and use their
protrusible stylet to feed either on epidermal cells or cells
deeper within the root.
• With the exception of species of a few genera that feed on
root tips, e.g. Belonolaimus, nematodes with this type of
feeding strategy generally cause little obvious tissue
damage .
Page 24
Cont..
• The migratory ectoparasites remove cytoplasm from the
parasitized cell, frequently causing their death and then
move to another cell to repeat the feeding process.
• Dorylaimid migratory ectoparasites include Trichodorus
spp, Xiphinema index and Longidorus elongatus while
Tylenchid migratory ectoparasite includes
Tylenchorhychus dubius. Psilenchus, Tylenchus and
Atylenchus feed only on root hairs.
Page 25
Sedentary ectoparasites
• These nematodes feed from a single site or plant cell for a
prolonged period of time while remaining outside the root.
• Sedentary ectoparasites such as Criconemella xenoplax use a
single feeding cell as a nutrient source for several days before the
nematode moves on to establish another feeding cell.
• Feeding by C. xenoplax causes little tissue damage compared to
Hemicycliophora arenaria and some Helicotylenchus species
(migratory ecto-endoparasites) which induce terminal galls when
feeding on the root tips.
• Hoplolaimus and Telotylenchus are semi-endoparasites feeding
internally and externally on plant root tissues.
Page 26
Endoparasites
• Endoparasitic nematodes invade the root tissue with part
or all of their body.
• Some feed soon after entering the root while others feed
only after migrating to a preferred feeding site (e.g. the
cortex and the xylem parenchyma cells).
Page 27
Migratory endoparasites
• Migratory endoparasites such as Pratylenchus,
Hirschmanniella and Radopholus spp, which have a small
but robust stylet enter the root and periodically feed as
they migrate intracellularly through the root tissue.
• They primarily inhabit the cortical tissue of the root and
retain their mobility and feed on the tissues as they move.
This causes extensive destruction of root tissue along the
path of the migrating nematode.
Page 28
Sedentary endoparasitic nematodes
• These are nematodes which
once they have reached the
feeding site inside plant cease
to be mobile and feed from a
fixed location.
• They include nematodes
species such as:
• Meloidogyne spp.
• Tylenchulus semipenetrans
• Rotylenchus spp.
Page 29
Sedentary endoparasitic nematodes
• These nematodes invade plant
tissue as newly hatched second-
stage juveniles- the infective
wormlike stage.
• They move through soil to locate
host then through plant tissue to
locate feeding site.
• Once at the feeding site the
female develops, remaining
permanently at the site for the
duration of her life.
Page 30
Sedentary endoparasitic nematodes
• As she develops her body
swell as a spherical, lemon,
kidney or ovoid form.
• Nematode feeds on relatively
small number of cells which
are regulated by the nematode
with growth substances.
• Cyst and root-knot nematodes
causes “giant” feeding cells to
form in the host plant.
Page 31
Sedentary endoparasitic nematodes
• Males remain in worm like shape
feeding on the surface of root for
a few days during which they
may or may not fertilize the
female before moving into the
soil where they die.
• Females produce large number
of eggs which remain in their
bodies (e.g. cyst nematodes –
Heterodera spp) accumulate in
egg masses (e.g. root-knot
nematodes –Meloidogyne spp.)
Attached to their bodies.
Page 32
Nematode shapes
• Nematode assume different shapes when alive and once
dead.
• These shapes vary in different nematode species too.
Swollen/ fusiform
Eg Nacobbus and Achysiella
Page 33
Nematodes shapes
Pear shape
E.g. Tylenchulus
 Lemon shape
• Heterodera
Page 34
Nematode shape
Kidney shaped Ribbed/ringed
• Criconematid
• Rotylenchulus
Page 35
Nematode shapes
Worm like
• Pratylenchus & Helicotylenchus
C-shaped
• Scutellonema
Page 36
Thank you

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Nematode biology

  • 2. Page 2 Content • Introduction • Appearance and structure • Life cycle • Nematode types Migratory endoparasites Sedentary endoparasites Ectoparasites • Nematode shapes
  • 3. Page 3 Introduction • Nematodes are a diverse group of worm-like animals. • They live virtually in every environment, both as parasites and as free-living organisms. • They are minute generally thus visible only under microscope. • Nematodes cause significant damage on crops leading to huge yield losses.
  • 4. Page 4 Appearance and structure • Plant parasitic nematodes are thread-like worms ranging from 0.25mm to >1.0mm long through some grow up to 4.0mm. • Nematodes vary in shape and sizes through most are known to taper towards the head and tail. • Females of some species are known to lose the worm-like shape as they mature and become enlarged taking a pear, lemon or kidney shape or spherical as adults. • Nematodes have circulatory, respiratory and digestive system like all animals.
  • 5. Page 5 Appearance and structure • Plant parasitic nematodes differ from nematodes which feed on bacteria and fungi in that they have a specialized feeding structure, a spear or stylet. • They use this stylet to inject enzymes into plant cells and tissue and then extract content in a similar way that aphids feed on plant.
  • 6. Page 6 Appearance and structure • The stylet is connected to the pharynx that, in turn, is connected to the intestine. • The intestine ends at the rectum in the female nematode and the cloaca in the male. • Attached to the pharynx are three - five salivary glands which produce secretions that may be emitted from the stylet and that assist the nematode in plant invasion and parasitism.
  • 7. Page 7 Appearance and structure • The pharynx is a muscular specialize area that can contact and expand the esophageal lining. • The expansions and contraction of the pharynx muscles allow the nematode to pump food into the intestine through its stylet or eject secretion fro its salivary glands into and around plant cells.
  • 8. Page 8 Appearance and structure • They have an outer skin or cuticle that is secreted from an inner hypodermis. • The muscles are attached longitudinally to the nematode’s hypodermis, allowing them to move only in the dorsal ventral direction (snake-like movement). • Inside the nematode there is an inner tube, the alimentary canal, which runs inside the nematode from head to tail.
  • 9. Page 9 Appearance and structure • Between the alimentary canal and the body wall is fluid that provides pressure against the wall to maintain body shape and allow movement. • In the middle to posterior of the nematode are the reproductive organs (vulva and spicule). • Nematode species often have both males and females, but it is not uncommon for plant nematodes to reproduce asexually by parthenogenesis.
  • 10. Page 10 Appearance and structure • The number of ovaries and position of vulva in female vary. • Male nematodes have one or two testes and they are easily identified by the presence of spicules.
  • 11. Page 11 Appearance and structure • Nematodes are triploblastic, containing three body layers (ectoderm, mesoderm and endoderm) in the embryo. • Nematodes have a body cavity that is not totally surrounded by mesoderm, so they are pseudocoelomic.
  • 12. Page 12 Structure and types of stylets • Nematodes have different stylets for which they use to obtain nutrients from their food sources. • There are two types of stylets namely: Stomato stylet- This is divided into 3 the knobs, shaft and cone plus a guiding ring in between the shaft and cone. Odonto stylet – This has also the 3 parts knob shaft and cone.
  • 13. Page 13 Stomato stylet The shaft and cone are separated by a guiding ring and restricts fluid movement from the nematode to the host the nematode feeds on. The knobs are larger compared to those of odonto stylet. E.g. Tylenchulus
  • 14. Page 14 Odonto stylet • These stylets lack the guiding ring between the shaft and cone and thus the nematode fluids are able to flow into the target host and thus able to transmit diseases e.g. plant viruses • E.g. Xiphinema
  • 15. Page 15 Life cycle • A nematode life cycle is divided into 6 stages namely: the egg, 4 juvenile stages and adult. • Duration of the stages differ in different species and also depending on factors such as temperature, moisture and plant host.
  • 16. Page 16 Cont.. • Nematodes are evolutionarily related to insects, and one feature they have in common is the requirement to molt between juvenile stages. • All nematodes undergo four molts from the juvenile to the adult phase of their life cycle. • They have four juvenile stages and an adult stage. • In many nematodes the first molt usually occurs in the egg and it is the second-stage juvenile that hatches.
  • 17. Page 17 Life cycle • Under favourable conditions in the tropics many species have relatively short life cycles. • This leads to several generations per each season thus rapid population build up. • Nematodes are known to survive unfavorable conditions such as dry season or cold winter in cyst form. • Different species are known to survive best at different stages e.g. Heterodera species survive best as egg encapsulated with cyst, Anguina species as second stage juvenile etc.
  • 18. Page 18 Nematode types • Nematodes can be separated into 2:  Aerial parasites i.e those which feed on above ground parts of plant. Root and tuber parasites i.e those which feed on below ground plant parts. • Nematodes can also be grouped by their feeding behavior and motility as follows: Migratory endoparasitic nematodes Sedentary endoparasites nematodes Ectoparasitic nematodes
  • 19. Page 19 Ectoparasites • Ectoparasites feed from root tissue by inserting their stylet from outside the root . • The group consists of several morphologically divergent families that have evolved different feeding strategies. • As a rule, species that have a short stylet feed on epidermal cells e.g. Tylenchorhychus dubius and those with long stylets feed on deeper tissues e.g. Belonolaimus, and Dolichodorus spp. Ectoparasites are either migratory or sedentary.
  • 20. Page 20 Ectoparasitic nematodes • These nematodes feed on the plant from the outside of the plant. • They feed externally on surface of the plant usually on root hairs or cortical tissue. • Often found in high densities though do not pose a problem. • They however cause serious damage if the plant is suffering abiotic or biotic stresses such as fungal attach or low water availability.
  • 21. Page 21 Ectoparasitic nematodes • Examples of ectoparasitic nematodes include: • Criconemoides spp. (ring nematodes) • Helicotylenchus spp.( spiral nematodes) • Aphelenchoides besseyi ( aerial rice white-tip nematodes) • Xiphinema spp.(dagger nematodes) • Longidorus spp. (needle nematodes) • Trichodorus & Paratrichodorus spp. (stunt nematodes). Trichodorus spp Criconemoides spp
  • 22. Page 22 Ectoparasitic nematodes • It has been found that some ectoparasitic nematodes transmit plant virus e.g. Longidorus spp, Xiphinema spp, Trichodorus spp and Paratrichodorus spp.
  • 23. Page 23 Migratory ectoparasites • These nematodes have the most primitive mode of parasitism; they remain outside of the root and use their protrusible stylet to feed either on epidermal cells or cells deeper within the root. • With the exception of species of a few genera that feed on root tips, e.g. Belonolaimus, nematodes with this type of feeding strategy generally cause little obvious tissue damage .
  • 24. Page 24 Cont.. • The migratory ectoparasites remove cytoplasm from the parasitized cell, frequently causing their death and then move to another cell to repeat the feeding process. • Dorylaimid migratory ectoparasites include Trichodorus spp, Xiphinema index and Longidorus elongatus while Tylenchid migratory ectoparasite includes Tylenchorhychus dubius. Psilenchus, Tylenchus and Atylenchus feed only on root hairs.
  • 25. Page 25 Sedentary ectoparasites • These nematodes feed from a single site or plant cell for a prolonged period of time while remaining outside the root. • Sedentary ectoparasites such as Criconemella xenoplax use a single feeding cell as a nutrient source for several days before the nematode moves on to establish another feeding cell. • Feeding by C. xenoplax causes little tissue damage compared to Hemicycliophora arenaria and some Helicotylenchus species (migratory ecto-endoparasites) which induce terminal galls when feeding on the root tips. • Hoplolaimus and Telotylenchus are semi-endoparasites feeding internally and externally on plant root tissues.
  • 26. Page 26 Endoparasites • Endoparasitic nematodes invade the root tissue with part or all of their body. • Some feed soon after entering the root while others feed only after migrating to a preferred feeding site (e.g. the cortex and the xylem parenchyma cells).
  • 27. Page 27 Migratory endoparasites • Migratory endoparasites such as Pratylenchus, Hirschmanniella and Radopholus spp, which have a small but robust stylet enter the root and periodically feed as they migrate intracellularly through the root tissue. • They primarily inhabit the cortical tissue of the root and retain their mobility and feed on the tissues as they move. This causes extensive destruction of root tissue along the path of the migrating nematode.
  • 28. Page 28 Sedentary endoparasitic nematodes • These are nematodes which once they have reached the feeding site inside plant cease to be mobile and feed from a fixed location. • They include nematodes species such as: • Meloidogyne spp. • Tylenchulus semipenetrans • Rotylenchus spp.
  • 29. Page 29 Sedentary endoparasitic nematodes • These nematodes invade plant tissue as newly hatched second- stage juveniles- the infective wormlike stage. • They move through soil to locate host then through plant tissue to locate feeding site. • Once at the feeding site the female develops, remaining permanently at the site for the duration of her life.
  • 30. Page 30 Sedentary endoparasitic nematodes • As she develops her body swell as a spherical, lemon, kidney or ovoid form. • Nematode feeds on relatively small number of cells which are regulated by the nematode with growth substances. • Cyst and root-knot nematodes causes “giant” feeding cells to form in the host plant.
  • 31. Page 31 Sedentary endoparasitic nematodes • Males remain in worm like shape feeding on the surface of root for a few days during which they may or may not fertilize the female before moving into the soil where they die. • Females produce large number of eggs which remain in their bodies (e.g. cyst nematodes – Heterodera spp) accumulate in egg masses (e.g. root-knot nematodes –Meloidogyne spp.) Attached to their bodies.
  • 32. Page 32 Nematode shapes • Nematode assume different shapes when alive and once dead. • These shapes vary in different nematode species too. Swollen/ fusiform Eg Nacobbus and Achysiella
  • 33. Page 33 Nematodes shapes Pear shape E.g. Tylenchulus  Lemon shape • Heterodera
  • 34. Page 34 Nematode shape Kidney shaped Ribbed/ringed • Criconematid • Rotylenchulus
  • 35. Page 35 Nematode shapes Worm like • Pratylenchus & Helicotylenchus C-shaped • Scutellonema