This document discusses several pigments found in muscle and blood tissues (myoglobin and hemoglobin), plant tissues (anthocyanins and carotenoids), and their structures, functions, and chemical properties. Myoglobin transports oxygen in muscle tissues, while hemoglobin carries oxygen in blood between the lungs and tissues. Anthocyanins are water-soluble pigments that give flowers, fruits, and vegetables their red and blue colors. Carotenoids are lipid-soluble pigments responsible for the yellow and red colors in plants and animals. The document outlines the molecular structures and chemical reactions of these important natural pigments.
Biochemical analysis of unknown bacteriaAerotolerance TeChantellPantoja184
Biochemical analysis of unknown bacteria
Aerotolerance Test
Fluid Thioglycollate broth (FTB) is a medium designed to test the aerotolerance of bacteria.
Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to water.
Methylene blue is an indicator that is colorless in an anaerobic environment and greenish-blue in the presence of oxygen.
The agar helps retard oxygen diffusion and helps maintain the stratification of organisms growing in different layers of the broth.
Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube.
Obligate aerobes will only grow in this oxygen-rich top layer. On another hand, obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones
Reactions typically take up to 1-2 days to develop at 37⁰C
Media is inoculated using an inoculating loop
(A) Escherichia coli and (C) Staphylococcus aureus: both are Facultative Anaerobe, grows both aerobically and anaerobically and growth is seen throughout the tube. Some are capable of growth respiring with oxygen and anaerobically by fermentation.
(B) Clostridium botulinum: Obligate Anaerobe: can not grow in the presence of oxygen, growth is seen approximately 1/4 to 1/2 of the way from the top of the tube.
(D) Neisseria sicca: Microaerophile, requires oxygen but at concentrations below atmosphere, grows just below the surface of the media but not at the top.
(E) Pseudomonas aeruginosa: Obligate Aerobe: oxygen is required for growth and grows at the top of the tube only. The Organism will “settle” and sink into the media if grown longer than 24 hrs.
Aerotolerance Test
Phenol red test
Phenol red broth is a differential test medium prepared as a base to which a carbohydrate such as sucrose, lactose, dextrose or glucose is added.
Included in the base medium are peptone and the pH indicator is phenol red. Phenol red is yellow below pH 6.8, pink to magenta above pH 7.4, and red in between. During preparation, the pH is adjusted to approximately 7.3 so it appears red.
Deamination of peptone amino acids produces ammonia which rises the pH and turns the broth pink.
An inverted Durham tube is added to each tube as an indicator of gas production.
Gas production, also from fermentation, is indicated by a bubble or pocket in the Durham tube where the broth has been displaced.
Acid production from fermentation of the carbohydrate lowers the pH below the neutral range of the indicator and turns the medium yellow. Deamination of peptone amino acids produces ammonia which rises the pH and ...
Plant pigments are coloured substances produced by the plants and are important in controlling photosynthesis. they are important for humans, arrtecting our attention and providing us with nutrients.
Introduction, classification, isolation, purification, biological activity of alkaloids, general methods of structural determination of alkaloids, structural elucidation of Morphine, Reserpine and Emetine
Rdp,udn and kinetics, Rumen undegradable protein, Rumen degradable protein and their kinetics, Sri Venkateswara veterinary university, Animal nutrition, Vishnu Vardhan Reddy
An amino acid is an organic molecule that is made up of a basic amino group (−NH2), an acidic carboxyl group (−COOH), and an organic R group (or side chain) that is unique to each amino acid. The term amino acid is short for α-amino [alpha-amino] carboxylic acid.
Steroids, Classification of Steroids, Cholesterol, Testosterone, Estrogen, Progesterone, Aldosterone, Significance, Biosynthesis, Skeleton structure, History, Bile acids, Cardiac Glycosides, Steroid hormones, Saponins, Mode of Action, Tests of steroids, Sterol, Adrenocorticosteroids, Glucocorticoids, Cortisol, Hydrocortisone, Prednisolone, Mineralocorticoids, Steroid Contraceptives, Androgens, Vitamin D2&D3, Cardenolides
All rights of images and videos are reserved to their rightful owners.
the presentation contain ways used to estimate proteins, this presentation prepared by TONNYBITE, a student from KILIMANJARO CHRISTIAN MEDICAL UNIVERSITY COLLEGE, TANZANIA
Biochemical analysis of unknown bacteriaAerotolerance TeChantellPantoja184
Biochemical analysis of unknown bacteria
Aerotolerance Test
Fluid Thioglycollate broth (FTB) is a medium designed to test the aerotolerance of bacteria.
Along with nutrients to support bacterial growth, it contains sodium thioglycollate, thioglycollic acid, L-cystine, methylene blue, and 0.05% agar.
The sodium thioglycollate, thioglycollic acid, and L-cystine reduce the oxygen to water.
Methylene blue is an indicator that is colorless in an anaerobic environment and greenish-blue in the presence of oxygen.
The agar helps retard oxygen diffusion and helps maintain the stratification of organisms growing in different layers of the broth.
Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube.
Obligate aerobes will only grow in this oxygen-rich top layer. On another hand, obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones
Reactions typically take up to 1-2 days to develop at 37⁰C
Media is inoculated using an inoculating loop
(A) Escherichia coli and (C) Staphylococcus aureus: both are Facultative Anaerobe, grows both aerobically and anaerobically and growth is seen throughout the tube. Some are capable of growth respiring with oxygen and anaerobically by fermentation.
(B) Clostridium botulinum: Obligate Anaerobe: can not grow in the presence of oxygen, growth is seen approximately 1/4 to 1/2 of the way from the top of the tube.
(D) Neisseria sicca: Microaerophile, requires oxygen but at concentrations below atmosphere, grows just below the surface of the media but not at the top.
(E) Pseudomonas aeruginosa: Obligate Aerobe: oxygen is required for growth and grows at the top of the tube only. The Organism will “settle” and sink into the media if grown longer than 24 hrs.
Aerotolerance Test
Phenol red test
Phenol red broth is a differential test medium prepared as a base to which a carbohydrate such as sucrose, lactose, dextrose or glucose is added.
Included in the base medium are peptone and the pH indicator is phenol red. Phenol red is yellow below pH 6.8, pink to magenta above pH 7.4, and red in between. During preparation, the pH is adjusted to approximately 7.3 so it appears red.
Deamination of peptone amino acids produces ammonia which rises the pH and turns the broth pink.
An inverted Durham tube is added to each tube as an indicator of gas production.
Gas production, also from fermentation, is indicated by a bubble or pocket in the Durham tube where the broth has been displaced.
Acid production from fermentation of the carbohydrate lowers the pH below the neutral range of the indicator and turns the medium yellow. Deamination of peptone amino acids produces ammonia which rises the pH and ...
Plant pigments are coloured substances produced by the plants and are important in controlling photosynthesis. they are important for humans, arrtecting our attention and providing us with nutrients.
Introduction, classification, isolation, purification, biological activity of alkaloids, general methods of structural determination of alkaloids, structural elucidation of Morphine, Reserpine and Emetine
Rdp,udn and kinetics, Rumen undegradable protein, Rumen degradable protein and their kinetics, Sri Venkateswara veterinary university, Animal nutrition, Vishnu Vardhan Reddy
An amino acid is an organic molecule that is made up of a basic amino group (−NH2), an acidic carboxyl group (−COOH), and an organic R group (or side chain) that is unique to each amino acid. The term amino acid is short for α-amino [alpha-amino] carboxylic acid.
Steroids, Classification of Steroids, Cholesterol, Testosterone, Estrogen, Progesterone, Aldosterone, Significance, Biosynthesis, Skeleton structure, History, Bile acids, Cardiac Glycosides, Steroid hormones, Saponins, Mode of Action, Tests of steroids, Sterol, Adrenocorticosteroids, Glucocorticoids, Cortisol, Hydrocortisone, Prednisolone, Mineralocorticoids, Steroid Contraceptives, Androgens, Vitamin D2&D3, Cardenolides
All rights of images and videos are reserved to their rightful owners.
the presentation contain ways used to estimate proteins, this presentation prepared by TONNYBITE, a student from KILIMANJARO CHRISTIAN MEDICAL UNIVERSITY COLLEGE, TANZANIA
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1. B. Myoglobin & Hemoglobin
Myoglobin is a complex muscle proteins
Hemoglobin is the blood pigment
• Hb contains 4 polypeptide chains & 4 heme groups, which
are planar collection of atoms with the iron atom at the center.
• Heme group function : to combine reversibly with a molecule
of O2 carried by the blood from the lungs to the tissues.
• Myoglobin a quarter its size compared to Hb; consists of a
single polypeptide chain (±150 AA units) attached to a single Hb
group; it is contained within the cell tissues & it acts as a
temporary storehouse for the O2 brought by the Hb in blood.
Hb considered the linking together of 4 myoglobins (the
discussion of these pigments can be limited to myoglobin)
2. Physical properties
• Oxygenation reaction
Myoglobin + molecular O2 oxymyoglobin (O2Mb) forms bright red
pigment
• Myoglobin is part of sarcoplasmic proteins of
muscle; soluble in water & dilute salt solution.
Chemical Properties
• Oxidation reaction
Myoglobin oxidation metmyoglobin (MMb) forms brown color
• Ferrous covalent complexes of myoglobin (purple) with :
- Molecular O2 oxymyoglobin
- Nitric oxide nitrosomyoglobin
- Carbonmonoxide carboxymyoglobin
3. Effect of Handling, Processing & Storage
• Cured Meat Pigment
In commercial practice, sodium nitrite (NaNO2) is the source of nitrous acid:
NaNO2 (salt cure) in water Na+ + NO2
- (nitrite ion) HNO2 (in the
curing brine)
Or using combustion gas (NO2) to smoke or gas-oven fresh meat:
2 NO2 (gas cure) + H2O (in meat) ---> HNO2 (nitrous acid) +
HNO3 (nitric acid)
Meat Curing:
HNO2 + Mb (myoglobin in meat) NOMb (pink cured meat pigment)
4. The formation of cured meat pigments viewed as 2 processes:
(1) Biochemical reaction, which reduce nitrite nitric oxide; iron in
heme the ferrous state
(2) Thermal denaturation of globin heating at 66 C or higher & may
involve the coprecipitation of the heme pigment with other protein
in meat
5. • Packaging
Because meat pigment easily reacts with oxygen to
produce either an acceptable oxygenated products
or unacceptable oxidized products
• Carbon monoxide (CO) flushing
It was done before sealing of fresh beef very effective for
preserving & stabilizing color for 15 days
Certain metallic ions (esp. Cu) extremely active in promoting
autooxidation of O2Mb to MMb, while Fe, Zn, Al are less active
6. C. Anthocyanins
A group of reddish water-soluble pigments in plants which exist
in the cell sap/juice, i.e. flowers, fruits, vegetables,
• An anthocyanin pigment is composed of an aglycone (an
anthocyanidin) esterified to 1 or more sugars. Only 5 type of sugars
found in it, which are, in order of relative abundance : glucose,
rhamnose, galactose, xylose, arabinose
• Anthocyanins may also be “acylated” which adds a third component
to the molecule, i.e. p-coumaric, ferulic, caffeic, malonic, vanillic, or
acetic acids may be esterified to the sugar molecule.
Fig. Anthocyanin aglicone
7. Stability in Food
• The addition of sulfite, sulfite oxide rapid bleaching of the
anthocyanins yellowish colors.
i.e. in the making of jams, preserves such as dried fruits &
vegetables
• Anthocyanins show a marked change in color with changed in pH
the higher the pH the faster the rate of destruction
Chemical Reactions
• The reaction with ascorbic acid the degradation of both
compounds the intermediate, peroxide produces by ascorbic acid
degradation
i.e. Cranberry juice cocktail stored at room temperature:
0 days – 9 mg/100 g anthocyanins & 18 mg/100 g ascorbic acid
6 months – ascorbic acid degradation & 80% degradation of anthocyanin
8. Group of anthocyanins:
– Chalcone
– Flavonones
– Flavones
– Flavonoids
– Flavonols
– Cathechins
– Anthocyanidins
9. Anthocyanidins which can be found in food
(red violet spectrum):
– Pelargonidin
– Cynaidin
– Delphinidin
– Peonidin
– Petunidin
– Malvidin
10. D. Carotenoids
A group of mainly lipid soluble compounds responsible of the
yellow & red colors of plants & animal products (430 – 480 nm).
• Most of produced carotenoids in nature is in form of fucoxanthin in
various algae, in green leaves : lutein, violaxanthin, neoxanthin; β-
carotene; lycopene in tomatoes; capxanthin in red peppers
LUTEIN
kiwi, egg yolk, corn, zucchini, red grapes, pumpkin
Good for
11. • Carotenoids include a class of HC, called carotenes, and their
oxygenated derivatives, called xanthophylls.
• They consist of 8 isoprenoids units joined in such a manner that the
arrangement of isoprenoid units is reversed in the center of the
molecule.
• Forms of carotenoids :
(1) free state in plant tissues (crystals or amorphous solids)
(2) solution in lipid media, i.e. capxanthin- lauric acid ester
in paprika
12. • The association of carotenoids with proteins stabilised the pigment
& also change the color, i.e. red carotenoid astaxanthin when
complexed with protein blue colorant in lobster shells; ovoverdin,
the green pigment in lobster eggs; carotenoid-protein complexes
found in fruits, vegetables.
• Carotenoids may occur in combination with reducing sugars via a
glycosidic bond, i.e. CROCIN - containing 2 molecules of the sugar
gentiobiose united with crocetin, found as the main pigment in
SAFFRON
Fig. Red pigment of astaxanthin Fig. Lobster
Fig. Mud crab
13. • Beta carotene is precursor of vitamin A, which yields 2 molecules
of vitamin A by cleavage at the center of the molecule.
• Stability of carotenoids depend on whether the pigment is in vivo
or in vitro in environmental condition, i.e. lycopene in tomatoes is
quite stable, but the extracted purified pigment is unstable.
Chemical Reactions
Provitamin A
• Alpha carotene is precursor of one molecule of vitamin A; which
is half identical to beta carotene.
Oxidation reaction
• Enzyme degraded carotenoids rapidly, i.e. lypoxygenase.
• In processed food heat, light, presence of pro- and antioxidant
influence carotenoids degradation.