The document discusses the Multiple Ovulation Embryo Transfer (MOET) technique used in dairy farming. MOET involves controlling the estrus cycles of donor and recipient cows, superovulating donor cows to produce multiple eggs, artificially inseminating donors, collecting embryos 7-8 days later, and transferring fresh or frozen embryos into recipients. This allows for increasing the herd size more quickly than traditional breeding since one donor cow can produce multiple embryos that can be implanted into various recipients.

1. MULTIPLE OVULATION EMBRYO
TRANSFER
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2. Dairy farming is a class of agriculture for
long-term production of milk, which is
processed for eventual sale of
a dairy product.
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3. In this world with increasing population,
demand for milk and milk products is
increasing day by day.
Cattles of desired breed is required in more
quantity.
Thus, MOET (Multiple Ovulation Embryo
Transfer) technique plays a key role in
increasing herd size in a given period of time.
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4.  Controlling oestrus in donor and recipient.
 Super ovulation in donor
 Inseminating the donor
 Collection of embryo from the donor
 Implantation of the collected donor
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5. Initial step
Controlling and synchronizing oestrus in
donor and recipient cows.
Cows can be given progesterone hormone
treatment like CIDRs or an implant into the
ear that will prevent oestrus.
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6. Super-ovulating the donor cows.
The donor cows are injected twice a day for
four days with a follicle stimulating hormone
(FSH) that super-ovulates them, increasing
the ovulation rate. Super-ovulating makes
them produce a lot more eggs (ova) than
normal.
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7. Care to be Taken
 Cows should be fed well prior to and after
calving
 Should have a sufficient interval after
calving – 6 weeks prior to start of program is
minimum
 Should have cycled
 Should Have been vet checked to ensure
they are clean
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8. Inseminating the donor cows.
 Donor cows are artificially inseminated in
the same way as most dairy cows are
inseminated.
 A pistolet is used to place the semen into
the reproductive tract of the cow.
(Artificial Intelligence)
 The semen is placed past the cervix into the
uterus.
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9. Embryo collection
 Few days after the fertilization has taken
place, the embryos travel down the fallopian
tubes.
 The donor cow is given an epidural injection
to relax her bowel.
 Her rear end is washed and sterilised.
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10.  The technician guides a catheter into the
vagina, through the cervix and up one side
(horn) of the uterus.
 An inflatable cuff on the catheter is filled
with air to hold the catheter in place and
fluid is run into the uterine horn.
 When the horn is filled with fluid it is run
back through the catheter and through a
very fine filter. This catches the embryos.
 After repeating this process several times
the catheter is transferred to the other
horn of the uterus and the process is
repeated.
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11. After collection, the cow is released to go
back to her paddock. She needs to be given a
Prostaglandin injection 3 days after to get
her cycling and to remove any embryos that
may have been left behind.
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12. The filter is rinsed into a petri dish and
searched under a microscope. Once found,
the embryos are counted, graded and
washed. The embryos can now be loaded into
transfer guns (similar to long AI pistolettes)
and transferred into recipients, or frozen.
Freezing takes about 2 hours with the
embryos held in straws of antifreeze
solution and slowly cooled to –30. They are
then plunged into liquid nitrogen and stored
indefinitely at –190 degrees C.
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13. Embryo transfer
Recipient cows are held in the race or head
bail, given an epidural and washed. The
ovaries are felt to check that the animals
have cycled successfully. As with flushing,
the transfer gun is inserted into the vagina,
passed through the cervix and up one of the
uterine horns where the embryo is
deposited.
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14. The Donor cow can again be used for super
ovulation in order to increase the herd
population.
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