The document evaluates the wound healing activity of Morinda tinctoria, a plant known for its therapeutic benefits in traditional medicine. The study utilizes both excision and incision wound models in rats to measure wound contraction and healing effectiveness of the plant extract compared to a standard drug. Significant pharmacological and phytochemical analyses were conducted to explore the potential of Morinda tinctoria in enhancing wound healing processes.

1. PHARMACOLOGICAL EVALUATION OF MORINDA
TINCTORA FOR WOUND HEALING ACTIVITY
MASTER OF PHARMACY
in
PHARMACOLOGY
Km Shweta
(2303000986008)
FACULTY OF PHARMACY
DR. APJ ABDUL KALAM TECHNICAL UNIVERSITY, LUCKNOW
(Formerly Uttar Pradesh Technical University, Lucknow)
SARASWATI HIGHER EDUCATION & TECHNICAL COLLEGE OF
PHARMACY, VARANASI
Under the Supervision of
Mr Prasant Kumar Singh
(Associate Professor)

2. INTRODUCTION
Wound healing: It is a dynamic and highly regulated process consisting of
cellular, humoral and molecular mechanisms. The normal cutaneous wound
healing process is a temporal process involving a complex series of overlapping
events, which can be divided into key stages including: hemorrhage and fibrin
clot formation, inflammatory response, reepithelialization, granulation tissue
formation, angiogenic response, connective tissue contraction, and remodelling.
Our skin is the key to our survival, sensing the environment, maintaining
physicochemical and thermal homeostasis, acting as a reservoir of essential
nutrients, providing passive and active defence, and responding to trauma and
injury. Maintaining these critical functions requires robust and effective
mechanisms to protect it from trauma and insult and to repair and replace
critical skin functions when damaged or lost.

3. PLANT PROFILE: Morinda tinctoria
Morinda tinctoria Roxb. (Family: Rubiaceae) is well known for its therapeutic benefit
in Indian systems of medicine including Ayurveda and Siddha and in other forms of
traditional Medicine worldwide for the treatment of several ailments. Almost all parts of
this plant have been explored for its medicinal uses. The expressed juice of leaves is
externally applied to gout to relieve pain. The leaves are administered internally as
a tonic and febrifuge. The n-Hexane, dichloromethane and methanol extracts of the
leaves were shown to possess antibacterial and antifungal activities.
Morinda tinctoria

4. Bombax Ceiba
Bombax ceiba linn comes from genus bombax also known as cotton tree or red
silk cotton tree. Bombax ceiba is a famous plant used extensively in traditional
medicine for various diseases. They are used in the treatment of cholera,
tubercular fistula, coughs, urinary complaints, nocturnal pollution, abdominal
pain due to dysentery, and impotency. The gum is astringent, demulcent and
tonic. It is used in the treatment of dysentery, haemoptysis in pulmonary
tuberculosis, influenza and menorrhagia. The leaves are hypotensive and
hypoglycaemic.
Bombax Ceiba

5. RESEARCH ENVISAGED
Morinda tinctoria Roxb. (Family: Rubiaceae) is is very well known for its
therapeutic benefit in Indian systems of medicine including Ayurveda and Siddha and
in other forms of traditional Medicine worldwide for the treatment of several
ailments. Almost all parts of this plant have been explored for its medicinal uses.
Bombax ceiba is used extensively in traditional medicine for various diseases, in
the treatment of cholera, tubercular fistula, coughs, urinary complaints, nocturnal
pollution, abdominal pain due to dysentery, and impotency. On the basis of previous
reported research work and traditional uses, the present study was aimed to evaluate
the Morinda tinctora for wound healing activity.

6. PLAN OF WORK
The study included the following.
 Literature Survey
 Collection and identification of plant material Morinda tinctora and Bombax Ceiba
 Plant extraction
 Pharmacognostic study of Morinda tinctora and Bombax Ceiba
 Physicochemical studies
 Phytochemical screening of Morinda tinctora and Bombax Ceiba extract
 Pharmacological Screening
 Acute toxicity studied
 Wound healing activity of Morinda tinctora and Bombax Ceiba extract
Excision wound model
Incision wound model
 Statistical Analysis

7. Excision wound model: The animals were divided into three groups with six in each
were anaesthetized by open mask method with anesthetic ether before wound
creation. The particular skin area was shaved 1 day prior to the experiment. An excision
wound was inflicted by cutting away a 300 mm2 full thickness of skin from a
predetermined shaved area. The wounds were left undressed to the open environment.
The ointment base, standard drug ointment (0.1% silver sulfadiazine) and methanolic
extract of Morinda tinctora extract ointment (5%, w/w) was applied topically to the
control group, standard group and treated group respectively, till the wound was
completely healed. In this model, wound contraction and epithelialization period was
monitored. Wound contraction was measured as percent contraction in each 2 days
after wound formation. From the healed wound, a specimen sample of tissue was
collected from each rat for histopathological examination

8. Incision wound model: In incision wound model, all the animals of each group were
anaesthetized under light ether anesthesia. Two full thickness paravertebral long incisions
were made through the skin at the distance of about 1 cm from midline on each side of the
depilated back of rat. After the incision was made the both edges of skin kept together and
stitched with black silk surgical thread (no. 000) and a curved needle (no. 11) was used for
stitching. The continuous threads on both wound edges were tightened for good closure of
the wound. After stitching, wound was left undressed then ointment base, standard
ointment and extracts ointment were applied daily up to 10 days; when wounds were cured
thoroughly the sutures were removed on the day 10 and tensile strength of cured wound
skin was measured using tensiometer

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11. THANK YOU