This document summarizes a study on monitoring and pest control of fruit flies in Thailand. The study had four objectives: 1) develop and test modified Steiner traps constructed from recycled water bottles, 2) investigate the diurnal activity of Bactrocera cucurbitae and Bactrocera dorsalis, 3) monitor for resistance to commonly used insecticides in B. cucurbitae, and 4) conduct a farmer survey on fruit fly management. The research was conducted in Thailand from July to August 2009. Traps were constructed for $0.30 each that could be used for monitoring and control. Results showed both fly species were most active in the morning, with B. dorsalis peaking from 7
Mass Production of Paecilomyces Lilacinus by using Different Cultivation Medi...Agriculture Journal IJOEAR
Paecilomyces lilacinus is a common saprophytic, filamentous fungus. Morphological characters of Paecilomyces lilacinus were separate mycelium, hyaline, conidia white to pink colored and formation of phialides. The growth of Paecilomyces lilacinus carried out on SDA media at room temperature was better than incubator. Various solid substrates like Rice, Wheat bran, and Sorghum were evaluated for the mass multiplication of fungus Paecilomyces lilacinus. Added dextrose and antibiotics in solid media for mass multiplication at room temperature. Among all the substrate Wheat bran recorded the maximum spore count of 7. 1 10-8 spore/ml followed by Sorghum 5. 4 10-8 spore/ml and Rice 5. 1 10-8 spore/ml after 20 days. Also dry mycelia weight or biomass of fungus Paecilomyces lilacinus without an incubator was more than using an incubator.
Testing the ability against Bacillus cereus of actinobacteria strains isolate...Agriculture Journal IJOEAR
This study aimed to test the antibacterial activity of Bacillus cereus of actinobacterial isolates isolated from marine sponges in the Kien Giang Sea, Vietnam. That can select the strains with high resistance to identify them. There were 198 actinobacterial isolates tested. Based on the ability of antimicrobial activity to B. cereus, 82/198 had the against B. cereus, in which there were six isolates with high (7.3%), 52 medium (25.6%), and 21 weak resistance (67.1%). Selection of six isolates with the best resistance to B. cereus (ND1.7a, ND2.7c, HD1-3e, HD1-6a, HD2.3b, and H6b) identified by PCR and 16S rRNA gene sequencing. The results identified five strains of Streptomyces (Streptomyces tateyamensis ND1.7a, Streptomyces althioticus HD1.3e, Streptomyces flaveolus HD1.6a, Streptomyces olivaceus HD2.3d, and Streptomyces albidoflavus H6b) and one strain of genus Microbacterium (Microbacterium tumbae ND2.7c).
Microbiological Investigations on Gryllotalpa Africana [Orthoptera: Gryllotal...iosrjce
This document summarizes a study that investigated the bacterial distribution on different external structures of the edible mole cricket Gryllotalpa africana in Nigeria. Ten bacterial genera were isolated including Bacillus, Staphylococcus, Proteus, Micrococcus, and Corynebacterium. Proteus was the most prevalent bacteria overall. Some Bacillus and Staphylococcus strains isolated from the cricket's skin including B. cereus and S. aureus present public health concerns as these bacteria can cause foodborne illness. While crickets are eaten in parts of Nigeria, the presence of potential pathogens indicates cooking is needed to eliminate health risks from consuming this insect.
Degradation of Nevirapine and Trimethoprim from Aqueous Solutions using Selec...Agriculture Journal IJOEAR
Together with pharmaceutical residues, personal care products encompassing prescription drugs, fragrances, and cosmetics have been detected in groundwater and other aquatic environments, hence compromising the quality of water. Their classification as micropollutants is due to their antibacterial resistance potential, persistence, and ecotoxicity. Biodegradation has been identified as a potential mechanism in their removal. The focus of this study focus was bioaugmentation; (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeroginosa) to enhance the degradation of Nevirapine and Trimethoprim in model aqueous solutions. A liquid chromatography-tandem mass spectrometer (LC-MS/MS) was used to determine the pharmaceuticals. The efficacy of the bacterial strains to degrade selected drugs was evaluated by making the two drugs the sole source of energy and carbon. From the experimental data, the highest percentage biodegradation was recorded; Pseudomonas aeroginosa (86 %) and Staphylococcus aureus (79 %) for TMP and NVP respectively.
Isolation and Screening of Soil Actinomycetes for Antimicrobial Activity OlakunleAjibola
Antimicrobial resistance in pathogens has greatly increased of late and now pose a serious public health problem globally. New antimicrobials are continuously needed to inhibit the growth of these resistant strains.
The aim of this study was to isolate and screen soil actinomycetes and evaluate their secondary metabolites for antimicrobial activities against selected pathogenic bacteria and fungi.
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
Effect of time and proportion of leaf harvest on pest, forage and root yields...Agriculture Journal IJOEAR
Dearth of knowledge exists regarding the leaf harvest intensity and frequency thresholds that support optimum forage and fresh storage root yields in Sierra Leone. A study was carried out to assess the effects of leaf harvesting time and proportion on Cylas puncticollis infestation, growth and yield of sweet potato in the inland valley swamp and upland ecologies of Njala. Treatment combinations comprised of two varieties ("Kabia" and "Gbanie"), four leaf harvest regimes: 0, 30 60 and 90 days after planting (DAP); and four-leaf harvest intensities (0, 25, 50 and 100%). The experiment was laid out in a randomized complete block design (RCBD) with three replications. Data collected included Cylas puncticollis severity on vines and storage roots, root dimensions and numbers, fresh foliage and storage root yields. The results revealed that leaf harvesting twice at 25 and 50% contributed more to optimum forage and storage root yields and related attributes of sweet potatoes compared to other treatments. The present study suggests that good agronomic management of sweet potato that supports optimum forage and storage root yields should be selected to meet the dual purpose for which it is grown. These findings serve as good guide for incorporation of leaf harvesting time, proportion of leaf harvest in germplasm assessment and new population development objectives.
Insecticidal and Anti-juvenile Hormone Activities of Precocene II against the...ijtsrd
The grasshopper Euprepocnemis plorans plorans caused a considerable damage to crops of the Nile Delta, Egypt. The present study was conducted aiming to assess the insecticidal and anti-hormonal effects of Precocene II on this grasshopper. The newly moulted 2nd or 4th (penultimate) instar nymphs were exposed to a series of doses: 60, 40, 20 and 10 -µg/cm2. Exposure of 2nd instar nymphs to the higher two doses resulted in complete mortality of nymphs within 24 h. At the lower two doses, PII exhibited a considerably extended low toxicity on the subsequently moulted instars and emerged adults. LD50 was calculated as 0.388 -µg/cm2. After exposure of the 4th instar nymphs to PII, no complete mortality was observed, but various mortality percentages among the treated nymphs, 5th instar nymphs and adults. LD50 was calculated as 17.022 -µg/cm2. PII exerted a slight inhibitory action on the nymphal growth of both 4th and 5th instars, after treatment of 2nd instar nymphs, regardless the dose level, but the growth rate was remarkably regressed after treatment of 4th instar nymphs with 40 and 20 -µg/cm2. Exposure of 2nd instar nymphs to PII led to 3.33% precociously moulted nymphs into 4th instar, skipping off the 3rd instar (only at the lowest dose). After exposure of 4th instar nymphs to PII, some treated nymphs precociously metamorphosed into adultoids, omitting the 5th instar, only at the higher tow doses. Another noticeable feature of the deranged development was permanent nymphs which induced in 2nd instar nymphs (3.85%) after exposure only to 20 -µg/cm2. Also, similar permanent nymphs were induced during the 4th instar. No permanent nymphs had been induced after exposure of 4th instar nymphs to PII. K. Ghoneim | A. Basiouny"Insecticidal and Anti-juvenile Hormone Activities of Precocene II against the Grasshopper Euprepocnemis plorans plorans (Charp.) (Orthoptera: Acrididae)." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-6 , October 2017, URL: http://www.ijtsrd.com/papers/ijtsrd4603.pdf http://www.ijtsrd.com/biological-science/other/4603/insecticidal-and-anti-juvenile-hormone-activities-of-precocene-ii-against-the-grasshopper-euprepocnemis-plorans-plorans-charp-orthoptera-acrididae/k-ghoneim
Mass Production of Paecilomyces Lilacinus by using Different Cultivation Medi...Agriculture Journal IJOEAR
Paecilomyces lilacinus is a common saprophytic, filamentous fungus. Morphological characters of Paecilomyces lilacinus were separate mycelium, hyaline, conidia white to pink colored and formation of phialides. The growth of Paecilomyces lilacinus carried out on SDA media at room temperature was better than incubator. Various solid substrates like Rice, Wheat bran, and Sorghum were evaluated for the mass multiplication of fungus Paecilomyces lilacinus. Added dextrose and antibiotics in solid media for mass multiplication at room temperature. Among all the substrate Wheat bran recorded the maximum spore count of 7. 1 10-8 spore/ml followed by Sorghum 5. 4 10-8 spore/ml and Rice 5. 1 10-8 spore/ml after 20 days. Also dry mycelia weight or biomass of fungus Paecilomyces lilacinus without an incubator was more than using an incubator.
Testing the ability against Bacillus cereus of actinobacteria strains isolate...Agriculture Journal IJOEAR
This study aimed to test the antibacterial activity of Bacillus cereus of actinobacterial isolates isolated from marine sponges in the Kien Giang Sea, Vietnam. That can select the strains with high resistance to identify them. There were 198 actinobacterial isolates tested. Based on the ability of antimicrobial activity to B. cereus, 82/198 had the against B. cereus, in which there were six isolates with high (7.3%), 52 medium (25.6%), and 21 weak resistance (67.1%). Selection of six isolates with the best resistance to B. cereus (ND1.7a, ND2.7c, HD1-3e, HD1-6a, HD2.3b, and H6b) identified by PCR and 16S rRNA gene sequencing. The results identified five strains of Streptomyces (Streptomyces tateyamensis ND1.7a, Streptomyces althioticus HD1.3e, Streptomyces flaveolus HD1.6a, Streptomyces olivaceus HD2.3d, and Streptomyces albidoflavus H6b) and one strain of genus Microbacterium (Microbacterium tumbae ND2.7c).
Microbiological Investigations on Gryllotalpa Africana [Orthoptera: Gryllotal...iosrjce
This document summarizes a study that investigated the bacterial distribution on different external structures of the edible mole cricket Gryllotalpa africana in Nigeria. Ten bacterial genera were isolated including Bacillus, Staphylococcus, Proteus, Micrococcus, and Corynebacterium. Proteus was the most prevalent bacteria overall. Some Bacillus and Staphylococcus strains isolated from the cricket's skin including B. cereus and S. aureus present public health concerns as these bacteria can cause foodborne illness. While crickets are eaten in parts of Nigeria, the presence of potential pathogens indicates cooking is needed to eliminate health risks from consuming this insect.
Degradation of Nevirapine and Trimethoprim from Aqueous Solutions using Selec...Agriculture Journal IJOEAR
Together with pharmaceutical residues, personal care products encompassing prescription drugs, fragrances, and cosmetics have been detected in groundwater and other aquatic environments, hence compromising the quality of water. Their classification as micropollutants is due to their antibacterial resistance potential, persistence, and ecotoxicity. Biodegradation has been identified as a potential mechanism in their removal. The focus of this study focus was bioaugmentation; (Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeroginosa) to enhance the degradation of Nevirapine and Trimethoprim in model aqueous solutions. A liquid chromatography-tandem mass spectrometer (LC-MS/MS) was used to determine the pharmaceuticals. The efficacy of the bacterial strains to degrade selected drugs was evaluated by making the two drugs the sole source of energy and carbon. From the experimental data, the highest percentage biodegradation was recorded; Pseudomonas aeroginosa (86 %) and Staphylococcus aureus (79 %) for TMP and NVP respectively.
Isolation and Screening of Soil Actinomycetes for Antimicrobial Activity OlakunleAjibola
Antimicrobial resistance in pathogens has greatly increased of late and now pose a serious public health problem globally. New antimicrobials are continuously needed to inhibit the growth of these resistant strains.
The aim of this study was to isolate and screen soil actinomycetes and evaluate their secondary metabolites for antimicrobial activities against selected pathogenic bacteria and fungi.
— Post-harvest diseases are a major problem for banana yield. Despite treatments with chemical fungicides, a persistence of diseases is noticed. This study aims at proposing a biological control method against banana post-harvest diseases by using banana rachis leachate. The effect of leachate has been tested in vitro on mycelial growth, conidial germination and in vivo on pathogenic fungi virulence. All leachate concentrations (5, 15 and 20%) tested showed antifungal activity on the tested fungi. However, the 20% concentration was more effective with complete inhibition of mycelial growth and conidial germination of all fungi. No symptoms of crown rot and anthracnose were observed after treatment of bananas with leachate. However, with azoxystrobin, the prevalence of crown rot and anthracnose was 60% and 30%, respectively. Banana rachis leachate recorded highly significant reduction of banana finger rot prevalence compared to azoxystrobin. Banana rachis leachate have strong antifungal properties that may be useful to control banana post-harvest disease as a safe alternative option to chemical fungicides Keywords— banana; post-harvest diseases; banana rachis leachate, antifungal activity.
Effect of time and proportion of leaf harvest on pest, forage and root yields...Agriculture Journal IJOEAR
Dearth of knowledge exists regarding the leaf harvest intensity and frequency thresholds that support optimum forage and fresh storage root yields in Sierra Leone. A study was carried out to assess the effects of leaf harvesting time and proportion on Cylas puncticollis infestation, growth and yield of sweet potato in the inland valley swamp and upland ecologies of Njala. Treatment combinations comprised of two varieties ("Kabia" and "Gbanie"), four leaf harvest regimes: 0, 30 60 and 90 days after planting (DAP); and four-leaf harvest intensities (0, 25, 50 and 100%). The experiment was laid out in a randomized complete block design (RCBD) with three replications. Data collected included Cylas puncticollis severity on vines and storage roots, root dimensions and numbers, fresh foliage and storage root yields. The results revealed that leaf harvesting twice at 25 and 50% contributed more to optimum forage and storage root yields and related attributes of sweet potatoes compared to other treatments. The present study suggests that good agronomic management of sweet potato that supports optimum forage and storage root yields should be selected to meet the dual purpose for which it is grown. These findings serve as good guide for incorporation of leaf harvesting time, proportion of leaf harvest in germplasm assessment and new population development objectives.
Insecticidal and Anti-juvenile Hormone Activities of Precocene II against the...ijtsrd
The grasshopper Euprepocnemis plorans plorans caused a considerable damage to crops of the Nile Delta, Egypt. The present study was conducted aiming to assess the insecticidal and anti-hormonal effects of Precocene II on this grasshopper. The newly moulted 2nd or 4th (penultimate) instar nymphs were exposed to a series of doses: 60, 40, 20 and 10 -µg/cm2. Exposure of 2nd instar nymphs to the higher two doses resulted in complete mortality of nymphs within 24 h. At the lower two doses, PII exhibited a considerably extended low toxicity on the subsequently moulted instars and emerged adults. LD50 was calculated as 0.388 -µg/cm2. After exposure of the 4th instar nymphs to PII, no complete mortality was observed, but various mortality percentages among the treated nymphs, 5th instar nymphs and adults. LD50 was calculated as 17.022 -µg/cm2. PII exerted a slight inhibitory action on the nymphal growth of both 4th and 5th instars, after treatment of 2nd instar nymphs, regardless the dose level, but the growth rate was remarkably regressed after treatment of 4th instar nymphs with 40 and 20 -µg/cm2. Exposure of 2nd instar nymphs to PII led to 3.33% precociously moulted nymphs into 4th instar, skipping off the 3rd instar (only at the lowest dose). After exposure of 4th instar nymphs to PII, some treated nymphs precociously metamorphosed into adultoids, omitting the 5th instar, only at the higher tow doses. Another noticeable feature of the deranged development was permanent nymphs which induced in 2nd instar nymphs (3.85%) after exposure only to 20 -µg/cm2. Also, similar permanent nymphs were induced during the 4th instar. No permanent nymphs had been induced after exposure of 4th instar nymphs to PII. K. Ghoneim | A. Basiouny"Insecticidal and Anti-juvenile Hormone Activities of Precocene II against the Grasshopper Euprepocnemis plorans plorans (Charp.) (Orthoptera: Acrididae)." Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-1 | Issue-6 , October 2017, URL: http://www.ijtsrd.com/papers/ijtsrd4603.pdf http://www.ijtsrd.com/biological-science/other/4603/insecticidal-and-anti-juvenile-hormone-activities-of-precocene-ii-against-the-grasshopper-euprepocnemis-plorans-plorans-charp-orthoptera-acrididae/k-ghoneim
This document summarizes a study that used 454 pyrosequencing to assess the effects of applying the entomopathogenic fungus Beauveria bassiana to soil on the diversity of the soil fungal community. B. bassiana was applied to an agricultural field plot in India and soil samples were collected from the treated plot and an untreated control plot over 7 weeks. Results showed that the overall fungal diversity was not influenced by the application of B. bassiana. Strain-specific markers indicated that the applied B. bassiana strain established in the treated plot and spread to the neighboring control plot. The study provides insights into the ecology of entomopathogenic fungi applied as biocontrol agents to soil environments.
Insecticide resistance in mosquitoes is a major challenge for controlling mosquito-borne diseases. Resistance has developed to all classes of insecticides used for indoor residual spraying and insecticide-treated bed nets. The primary mechanisms of resistance include target-site insensitivity and increased activity of detoxifying enzymes. Resistance monitoring is inadequate in most areas and resistance genes can spread rapidly in mosquito populations, undermining vector control efforts. Integrated approaches are needed to manage resistance, including moderating insecticide use, saturating populations, and using multiple insecticide classes.
This presentation discusses the resurgence of mites. It defines resurgence as an increase in pest populations following insecticide applications that kill natural enemies. There are two types of resurgence: primary pest resurgence, where the target pest population increases after treatment; and secondary pest resurgence, where a minor pest becomes a major pest after its natural enemies are killed. Case studies show specific examples like imidacloprid insecticide causing spider mite outbreaks by debilitating predators, and synthetic pyrethroids inducing spider mite resurgences by killing phytoseiid predators. Management of resurgence involves integrating cultural, biological and chemical control methods to preserve natural enemies.
Effect of Compost Extract Fortified with Tempe on Chili Mosaic Virus DiseaseIJEABJ
Mosaic disease caused by multiple infections of viruses in one of the most devastating virus diseases of chili pepper (Capsicum annuum) in Indonesia. Improving plant resistance by treatment with exogenous bioactive compounds is promisingly developed for plant protection in organic chili production. We demonstrated the suppressive effects of a fermented water extract of compost fortified with over-fermented tempe (TCE) on mosaic disease and its aphid vector. TCE was applied weekly by foliar spraying at 0.2 and 2.0 % on potted Capsicum growing in field. The result showed that treated plant sprayed with TNF preparation exhibited a significantly slower disease progression as represented by a lower area under disease progress curve compared to control plant. Disease suppression was obtained at concentration as low as 0.2%. TCE-treated plants were significantly less colonized by Aphis gossypii than control plant.
This presentation emphasizes development of resistance in insects against insecticides with different mechanisms and metabolic pathways along with some research findings. it also includes resistance management with different strategies.
This document discusses the use of nano-agrochemicals in pest management and compares their mode of action to organophosphate (OP) compounds. It notes that nano-agrochemicals can have multiple modes of action, including inducing oxidative stress, damaging insect guts and cuticles, and acting as slow-release formulations. Their high surface area allows them to be more effective at lower doses than OP compounds, which primarily target the nervous system as nerve poisons. While nano-agrochemicals offer benefits like increased efficacy, OP compounds present more risks to non-target organisms from their narrow and acute mode of action.
The document discusses the diamondback moth (DBM), Plutella xylostella, which is the most destructive pest of cabbage in the Philippines. It notes that DBM has developed high levels of resistance to most insecticides used for its control. It provides background on various diamide insecticides such as flubendiamide and chlorantraniliprole that have been used against DBM. However, field failures have been reported shortly after their introduction, indicating the development of resistance. The document emphasizes the importance of monitoring baseline susceptibility and implementing insecticide resistance management strategies to preserve the effectiveness of new insecticide classes.
Biological Control Mechanism- by KanishKanish Sarker
Fluorescent pseudomonads can be used as biological control agents against plant pathogens through several mechanisms. They produce siderophores like pyoverdin and pyochelin that compete with pathogens for iron. Secondary metabolites like 2,4-diacetylphloroglucinol (DAPG), pyrrolnitrin, and phenazines also inhibit pathogens. Some fluorescent pseudomonads induce systemic resistance in plants via the jasmonic acid/ethylene pathway. Understanding the regulation of traits involved in biological control can help improve the use of fluorescent pseudomonads as sustainable alternatives to chemicals for controlling plant diseases.
This document outlines the objectives and methodology of a seminar on seasonal occurrence of soybean insect pests and the relative bioefficacy of different insecticides. The objectives are to: 1) Record the seasonal occurrence of soybean insect pests, 2) Evaluate the efficacy of different insecticides against major soybean pests, and 3) Determine the benefit-cost ratio of insecticidal treatments. The methodology involves monitoring pest populations over time, testing insecticides in RBD plots, and calculating costs and yields to determine benefit-cost ratios of treatments.
Effect of Sakkara Brewing on the Severity of Powdery Mildew Disease of Luffa ...AI Publications
Powdery mildew is one of the major production constraint of cucurbits in almost all parts of Sri Lanka.The disease can be controlled with fungicides. However, bio control agents or organic compounds provide economically sound, practically feasible and environmentally safe approach. “Sakkaraa” brewing (SBr) is a fermented aqueous drink based on cane sugar and yeast (Saccharomyces cerevisiae). Most of the studies assessing the efficacy of yeast as a bio control agent, however, have focused on its effects against some fungi. Two experiments were conducted in parallel to identify the effect of SBr on severity of powdery mildew of Luffa and cucumber varieties under greenhouse condition. Six luffa varieties and twelve cucumber varieties were grown in pots and artificially inoculated with powdery mildew pathogen. Layout of the factorial experiments involving crop varieties and SBr treatments was completely randomized block design with four replications. About 15 days after inoculation of spore suspension of pathogen and when powdery mildew symptoms were well appeared, started the application of diluted SBr on six Luffa varieties and twelve cucumber varieties as an aqueous spray and untreated plants of each Luffa and cucumber variety were kept as control. Disease evaluation and measurements of percentage disease severity index (DSI (%)) of powdery mildew on plants were performed and recorded at flowering stage and fruiting stage. Microscopic observations confirmed that causal agent of powdery mildew of Luffa and cucumber in the country was Podosphaera xanthii. Results of DSI % of powdery mildew in both experiment showed that there was a significant difference between SBr applied treatment and control both at flowering and fruiting stage. Luffa varieties did not show significantly different of DSI (%) of powdery mildew. Popular Luffa variety Naga recorded highest DSI (%) when compared with other tested varieties. Cucumber varieties showed significant difference of DSI (%) of powdery mildew at flowering and fruiting stages. Cucumber var. KWxG17(S) Green and Var. Tunnel Green showed significantly lower DSI (%) of powdery mildew compared to other tested varieties. Results revealed that SBr has remarkable ability of control of powdery mildew and provides an opportunity to produce an effective control tool to protect Luffa and cucumber varieties from powdery mildew disease.
Insecticide resistance management strategies in Stored grain pestsramya sri nagamandla
References
Champ, B.R., Dyte, C.E., 1976. Report of the FAO global survey of pesticide susceptibility of stored grain pests. FAO Plant Production and Protection Series, No. 5, p.297.
Collins, P.J., 1996 – 2006. Unpublished annual reports to the National Working Party on Grain Protection, Australia.
Collins, P.J., Wilson, D., 1987. Efficacy of current and potential grain protectant insecticides against fenitrothion-resistant strain of the sawtoothed grain beetle, Oryzaephilus surinamensis, L. Pesticide Science 20, 93-104.
Collins, P.J., Daglish, G.J., Pavic, H., Kopittke, K.A., 2005. Response of mixed-age cultures of phosphine-resistant and susceptible strains of the lesser grain borer, Rhyzopertha dominica, to phosphine at a range of concentrations and exposure periods. Journal of Stored Products Research 41, 373-385.
Collins, P.J., Emery, R.N., Wallbank, B.E., 2003. Two decades of monitoring and managing phosphine resistance in Australia. In: Proceedings of the 8th International Working Conference on Stored Product Protection, July 2002, York, UK, pp 570-575.
Collins, P.J., Lambkin, T.M., Bridgeman, B.W., Pulvirenti, C., 1993. Resistance to grain-protectant insecticides in coleopterous pests of stored cereals in Queensland, Australia. Journal of Economic Entomology 86, 239-245.
Heather, N.W., Wilson, D., 1983. Resistance to fenitrothion in Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) in Queensland. Journal of Australian Entomological Society 22, 210.
Lorini, I., Collins, P.J., Daglish, G.J., Nayak, M.K., Pavic, H., in press. Detection and Characterisation of strong resistance to phosphine in Brazilian Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). Pest Management Science.
Nayak, M.K., Collins, P.J., Pavic, H., 2003. Developments in phosphine resistance in China and possible implications for Australia. In: Stored grain in Australia 2003, proceedings of the Australian Postharvest Technical Conference, Canberra 25-27 June 2003.
Nayak, M.K., Daglish, G.J., Byrne, V.S., 2005. Effectiveness of spinosad as a grain protectant against resistant beetle and psocid pests of stored grain in Australia. Journal of Stored Products Research 41, 455-467.
Schlipalius, D.I., Cheng, Q., Reilly, P.E.B., Collins, P.J., Ebert, P.R., 2002. Genetic linkage analysis of the lesser grain borer Rhyzopertha dominica identifies two loci that confer high-level resistance to the fumigant phosphine. Genetics 161, 773-782.
This document discusses the role of chemical ecology, volatile compounds, and secondary plant substances in plant resistance to insects. It provides background on chemical ecology and how plant chemicals can affect insect behavior. Secondary plant metabolites like alkaloids, terpenoids, and phenolic compounds adversely impact insect growth, development, and metabolism. Specific examples are given of compounds that act as feeding deterrents or have toxic effects on insects. The document also discusses how plant volatile compounds can attract or repel insects.
Selection and Efficacy Biocontrol Agents in Vitro against Fire Blight (Erwini...IRJET Journal
This document summarizes a study that evaluated the effectiveness of biological control agents against the fire blight pathogen Erwinia amylovora in vitro. 114 antagonist bacterial and yeast isolates were tested for their ability to inhibit the growth of E. amylovora. Nine bacterial strains showed satisfactory inhibition (greater than or equal to 20mm) and the best inhibition percentages of 31-41%. These nine strains were generally bacteriolytic, meaning they induced lysis of the pathogen. The study aimed to contribute to fire blight control strategies by selecting effective antagonist microbes that could be developed as biological control agents.
1) A study investigated Pseudomonas aeruginosa isolated from 65 burn victims admitted to a hospital in Iraq over 2 months.
2) PCR and phenotypic assays found that the majority of P. aeruginosa isolates were able to form alginate biofilm and had high antibiotic multi-drug resistance.
3) Specifically, 82% of isolates were found to be positive for alginate biofilm formation by PCR and 91% by a phenotypic assay, and the isolates showed resistance to many antibiotics with a multiple antibiotic resistance index of 0.4.
This document summarizes mechanisms of insecticide resistance in insects and its management. It discusses how resistance can develop due to behavioral adaptations, reduced penetration of insecticides, target site insensitivity, and enhanced detoxification. Target site insensitivity can occur via nerve insensitivity, acetylcholinesterase insensitivity, or reduced binding at midgut target sites. Enhanced detoxification involves increased activity of enzymes like cytochrome P450s, carboxylesterases, and glutathione S-transferases. Case studies provide examples of resistance developing in insect populations exposed to insecticides over multiple generations. Effective resistance management requires using a combination of control strategies.
Fungi-Nematode Interaction: Diversity, Ecology and Bio-control Prospects in A...UASR, UASD
Nematodes in the genera Aphelenchus, Aphelenchoides, Ditylenchus and Tylenchus are among the most common fungivorous nematodes
Fungivorous nematodes feed on a diversity of soil fungi, including saprophytic, plant-pathogenic and plant-beneficial (such as mycorrhizal) fungi and are known as polyphagous nematodes
This study evaluated endophytic bacteria from Pinus taeda L. as potential biocontrol agents of Fusarium circinatum, the causal agent of pitch canker disease affecting pine seedlings. Five bacterial strains - four Bacillus subtilis and one Burkholderia sp. isolated from healthy pine tissue - were tested against F. circinatum in dual culture experiments. All bacteria inhibited the fungal pathogen, arresting mycelial growth within 1 cm. Thermostable metabolites from the bacterial cultures also significantly reduced fungal growth over 50% when added to media. These endophytic bacteria adapted to pine tissues showed potential as biocontrol agents to control the pitch canker fungus in nurseries and prevent disease spread.
This document describes a study on the biological control of bacterial onion diseases using the bacterium Pantoea agglomerans strain 2066-7. The study involved screening 77 microorganisms isolated from different plant and environmental sources for antagonistic activity against four bacterial onion pathogens (Pseudomonas marginalis, Pseudomonas viridiflava, Xanthomonas retroflexus, and Pantoea ananatis). Pantoea agglomerans strain 2066-7 showed the strongest antagonistic activity against all four pathogens in vitro. Further experiments found that strain 2066-7 provided complete control of P. marginalis and P. viridiflava at a concentration of 107 CFU/ml,
Efficacy of bacterial bioagents in the management of plant diseasesShikha Sharma
This document summarizes the use of bacterial biocontrol agents for managing plant diseases. It discusses three important bacterial species - Bacillus subtilis, Pseudomonas fluorescens, and Pseudomonas aeruginosa. For each species, it provides the taxonomic classification and describes their morphological characteristics. It also outlines their modes of action, which includes production of antibiotics, siderophores, and inducing systemic resistance in plants. Several studies demonstrating the effectiveness of these bacteria in reducing plant diseases are summarized through tables and figures. The document concludes that bacterial biocontrol agents can offer an ecofriendly and cost-effective strategy for plant disease management.
This document discusses insecticide resistance in insects. It begins by defining insecticide resistance as the ability of some insects to survive exposure to an insecticide that would normally kill them. The document then discusses the history of reported insecticide resistance dating back to 1914. It notes that over 500 insect species have now developed resistance. The mechanisms of resistance include metabolic resistance, target site resistance, behavioral resistance, and penetration resistance. Factors that influence the development of resistance include the frequency of insecticide application and the intensity of selection pressure from the insecticide.
This study aims to evaluate the efficacy of extracts from Origanum Vulgare (oregano) as a mosquito repellent. Specifically, it seeks to determine how long the repellency lasts, compare its effectiveness to synthetic repellents, and rate its efficacy on a scale of 1-10. The study hypothesizes that the repellency will last 30 minutes to 1 hour depending on the amount of extract used, that the organic repellent will be more effective than synthetic options, and that it will rate an 8 on the effectiveness scale. If conducted, the study would use an experimental design to test these hypotheses and help identify a safe and effective natural mosquito repellent.
This document summarizes a study that used 454 pyrosequencing to assess the effects of applying the entomopathogenic fungus Beauveria bassiana to soil on the diversity of the soil fungal community. B. bassiana was applied to an agricultural field plot in India and soil samples were collected from the treated plot and an untreated control plot over 7 weeks. Results showed that the overall fungal diversity was not influenced by the application of B. bassiana. Strain-specific markers indicated that the applied B. bassiana strain established in the treated plot and spread to the neighboring control plot. The study provides insights into the ecology of entomopathogenic fungi applied as biocontrol agents to soil environments.
Insecticide resistance in mosquitoes is a major challenge for controlling mosquito-borne diseases. Resistance has developed to all classes of insecticides used for indoor residual spraying and insecticide-treated bed nets. The primary mechanisms of resistance include target-site insensitivity and increased activity of detoxifying enzymes. Resistance monitoring is inadequate in most areas and resistance genes can spread rapidly in mosquito populations, undermining vector control efforts. Integrated approaches are needed to manage resistance, including moderating insecticide use, saturating populations, and using multiple insecticide classes.
This presentation discusses the resurgence of mites. It defines resurgence as an increase in pest populations following insecticide applications that kill natural enemies. There are two types of resurgence: primary pest resurgence, where the target pest population increases after treatment; and secondary pest resurgence, where a minor pest becomes a major pest after its natural enemies are killed. Case studies show specific examples like imidacloprid insecticide causing spider mite outbreaks by debilitating predators, and synthetic pyrethroids inducing spider mite resurgences by killing phytoseiid predators. Management of resurgence involves integrating cultural, biological and chemical control methods to preserve natural enemies.
Effect of Compost Extract Fortified with Tempe on Chili Mosaic Virus DiseaseIJEABJ
Mosaic disease caused by multiple infections of viruses in one of the most devastating virus diseases of chili pepper (Capsicum annuum) in Indonesia. Improving plant resistance by treatment with exogenous bioactive compounds is promisingly developed for plant protection in organic chili production. We demonstrated the suppressive effects of a fermented water extract of compost fortified with over-fermented tempe (TCE) on mosaic disease and its aphid vector. TCE was applied weekly by foliar spraying at 0.2 and 2.0 % on potted Capsicum growing in field. The result showed that treated plant sprayed with TNF preparation exhibited a significantly slower disease progression as represented by a lower area under disease progress curve compared to control plant. Disease suppression was obtained at concentration as low as 0.2%. TCE-treated plants were significantly less colonized by Aphis gossypii than control plant.
This presentation emphasizes development of resistance in insects against insecticides with different mechanisms and metabolic pathways along with some research findings. it also includes resistance management with different strategies.
This document discusses the use of nano-agrochemicals in pest management and compares their mode of action to organophosphate (OP) compounds. It notes that nano-agrochemicals can have multiple modes of action, including inducing oxidative stress, damaging insect guts and cuticles, and acting as slow-release formulations. Their high surface area allows them to be more effective at lower doses than OP compounds, which primarily target the nervous system as nerve poisons. While nano-agrochemicals offer benefits like increased efficacy, OP compounds present more risks to non-target organisms from their narrow and acute mode of action.
The document discusses the diamondback moth (DBM), Plutella xylostella, which is the most destructive pest of cabbage in the Philippines. It notes that DBM has developed high levels of resistance to most insecticides used for its control. It provides background on various diamide insecticides such as flubendiamide and chlorantraniliprole that have been used against DBM. However, field failures have been reported shortly after their introduction, indicating the development of resistance. The document emphasizes the importance of monitoring baseline susceptibility and implementing insecticide resistance management strategies to preserve the effectiveness of new insecticide classes.
Biological Control Mechanism- by KanishKanish Sarker
Fluorescent pseudomonads can be used as biological control agents against plant pathogens through several mechanisms. They produce siderophores like pyoverdin and pyochelin that compete with pathogens for iron. Secondary metabolites like 2,4-diacetylphloroglucinol (DAPG), pyrrolnitrin, and phenazines also inhibit pathogens. Some fluorescent pseudomonads induce systemic resistance in plants via the jasmonic acid/ethylene pathway. Understanding the regulation of traits involved in biological control can help improve the use of fluorescent pseudomonads as sustainable alternatives to chemicals for controlling plant diseases.
This document outlines the objectives and methodology of a seminar on seasonal occurrence of soybean insect pests and the relative bioefficacy of different insecticides. The objectives are to: 1) Record the seasonal occurrence of soybean insect pests, 2) Evaluate the efficacy of different insecticides against major soybean pests, and 3) Determine the benefit-cost ratio of insecticidal treatments. The methodology involves monitoring pest populations over time, testing insecticides in RBD plots, and calculating costs and yields to determine benefit-cost ratios of treatments.
Effect of Sakkara Brewing on the Severity of Powdery Mildew Disease of Luffa ...AI Publications
Powdery mildew is one of the major production constraint of cucurbits in almost all parts of Sri Lanka.The disease can be controlled with fungicides. However, bio control agents or organic compounds provide economically sound, practically feasible and environmentally safe approach. “Sakkaraa” brewing (SBr) is a fermented aqueous drink based on cane sugar and yeast (Saccharomyces cerevisiae). Most of the studies assessing the efficacy of yeast as a bio control agent, however, have focused on its effects against some fungi. Two experiments were conducted in parallel to identify the effect of SBr on severity of powdery mildew of Luffa and cucumber varieties under greenhouse condition. Six luffa varieties and twelve cucumber varieties were grown in pots and artificially inoculated with powdery mildew pathogen. Layout of the factorial experiments involving crop varieties and SBr treatments was completely randomized block design with four replications. About 15 days after inoculation of spore suspension of pathogen and when powdery mildew symptoms were well appeared, started the application of diluted SBr on six Luffa varieties and twelve cucumber varieties as an aqueous spray and untreated plants of each Luffa and cucumber variety were kept as control. Disease evaluation and measurements of percentage disease severity index (DSI (%)) of powdery mildew on plants were performed and recorded at flowering stage and fruiting stage. Microscopic observations confirmed that causal agent of powdery mildew of Luffa and cucumber in the country was Podosphaera xanthii. Results of DSI % of powdery mildew in both experiment showed that there was a significant difference between SBr applied treatment and control both at flowering and fruiting stage. Luffa varieties did not show significantly different of DSI (%) of powdery mildew. Popular Luffa variety Naga recorded highest DSI (%) when compared with other tested varieties. Cucumber varieties showed significant difference of DSI (%) of powdery mildew at flowering and fruiting stages. Cucumber var. KWxG17(S) Green and Var. Tunnel Green showed significantly lower DSI (%) of powdery mildew compared to other tested varieties. Results revealed that SBr has remarkable ability of control of powdery mildew and provides an opportunity to produce an effective control tool to protect Luffa and cucumber varieties from powdery mildew disease.
Insecticide resistance management strategies in Stored grain pestsramya sri nagamandla
References
Champ, B.R., Dyte, C.E., 1976. Report of the FAO global survey of pesticide susceptibility of stored grain pests. FAO Plant Production and Protection Series, No. 5, p.297.
Collins, P.J., 1996 – 2006. Unpublished annual reports to the National Working Party on Grain Protection, Australia.
Collins, P.J., Wilson, D., 1987. Efficacy of current and potential grain protectant insecticides against fenitrothion-resistant strain of the sawtoothed grain beetle, Oryzaephilus surinamensis, L. Pesticide Science 20, 93-104.
Collins, P.J., Daglish, G.J., Pavic, H., Kopittke, K.A., 2005. Response of mixed-age cultures of phosphine-resistant and susceptible strains of the lesser grain borer, Rhyzopertha dominica, to phosphine at a range of concentrations and exposure periods. Journal of Stored Products Research 41, 373-385.
Collins, P.J., Emery, R.N., Wallbank, B.E., 2003. Two decades of monitoring and managing phosphine resistance in Australia. In: Proceedings of the 8th International Working Conference on Stored Product Protection, July 2002, York, UK, pp 570-575.
Collins, P.J., Lambkin, T.M., Bridgeman, B.W., Pulvirenti, C., 1993. Resistance to grain-protectant insecticides in coleopterous pests of stored cereals in Queensland, Australia. Journal of Economic Entomology 86, 239-245.
Heather, N.W., Wilson, D., 1983. Resistance to fenitrothion in Oryzaephilus surinamensis (L.) (Coleoptera: Silvanidae) in Queensland. Journal of Australian Entomological Society 22, 210.
Lorini, I., Collins, P.J., Daglish, G.J., Nayak, M.K., Pavic, H., in press. Detection and Characterisation of strong resistance to phosphine in Brazilian Rhyzopertha dominica (F.) (Coleoptera: Bostrychidae). Pest Management Science.
Nayak, M.K., Collins, P.J., Pavic, H., 2003. Developments in phosphine resistance in China and possible implications for Australia. In: Stored grain in Australia 2003, proceedings of the Australian Postharvest Technical Conference, Canberra 25-27 June 2003.
Nayak, M.K., Daglish, G.J., Byrne, V.S., 2005. Effectiveness of spinosad as a grain protectant against resistant beetle and psocid pests of stored grain in Australia. Journal of Stored Products Research 41, 455-467.
Schlipalius, D.I., Cheng, Q., Reilly, P.E.B., Collins, P.J., Ebert, P.R., 2002. Genetic linkage analysis of the lesser grain borer Rhyzopertha dominica identifies two loci that confer high-level resistance to the fumigant phosphine. Genetics 161, 773-782.
This document discusses the role of chemical ecology, volatile compounds, and secondary plant substances in plant resistance to insects. It provides background on chemical ecology and how plant chemicals can affect insect behavior. Secondary plant metabolites like alkaloids, terpenoids, and phenolic compounds adversely impact insect growth, development, and metabolism. Specific examples are given of compounds that act as feeding deterrents or have toxic effects on insects. The document also discusses how plant volatile compounds can attract or repel insects.
Selection and Efficacy Biocontrol Agents in Vitro against Fire Blight (Erwini...IRJET Journal
This document summarizes a study that evaluated the effectiveness of biological control agents against the fire blight pathogen Erwinia amylovora in vitro. 114 antagonist bacterial and yeast isolates were tested for their ability to inhibit the growth of E. amylovora. Nine bacterial strains showed satisfactory inhibition (greater than or equal to 20mm) and the best inhibition percentages of 31-41%. These nine strains were generally bacteriolytic, meaning they induced lysis of the pathogen. The study aimed to contribute to fire blight control strategies by selecting effective antagonist microbes that could be developed as biological control agents.
1) A study investigated Pseudomonas aeruginosa isolated from 65 burn victims admitted to a hospital in Iraq over 2 months.
2) PCR and phenotypic assays found that the majority of P. aeruginosa isolates were able to form alginate biofilm and had high antibiotic multi-drug resistance.
3) Specifically, 82% of isolates were found to be positive for alginate biofilm formation by PCR and 91% by a phenotypic assay, and the isolates showed resistance to many antibiotics with a multiple antibiotic resistance index of 0.4.
This document summarizes mechanisms of insecticide resistance in insects and its management. It discusses how resistance can develop due to behavioral adaptations, reduced penetration of insecticides, target site insensitivity, and enhanced detoxification. Target site insensitivity can occur via nerve insensitivity, acetylcholinesterase insensitivity, or reduced binding at midgut target sites. Enhanced detoxification involves increased activity of enzymes like cytochrome P450s, carboxylesterases, and glutathione S-transferases. Case studies provide examples of resistance developing in insect populations exposed to insecticides over multiple generations. Effective resistance management requires using a combination of control strategies.
Fungi-Nematode Interaction: Diversity, Ecology and Bio-control Prospects in A...UASR, UASD
Nematodes in the genera Aphelenchus, Aphelenchoides, Ditylenchus and Tylenchus are among the most common fungivorous nematodes
Fungivorous nematodes feed on a diversity of soil fungi, including saprophytic, plant-pathogenic and plant-beneficial (such as mycorrhizal) fungi and are known as polyphagous nematodes
This study evaluated endophytic bacteria from Pinus taeda L. as potential biocontrol agents of Fusarium circinatum, the causal agent of pitch canker disease affecting pine seedlings. Five bacterial strains - four Bacillus subtilis and one Burkholderia sp. isolated from healthy pine tissue - were tested against F. circinatum in dual culture experiments. All bacteria inhibited the fungal pathogen, arresting mycelial growth within 1 cm. Thermostable metabolites from the bacterial cultures also significantly reduced fungal growth over 50% when added to media. These endophytic bacteria adapted to pine tissues showed potential as biocontrol agents to control the pitch canker fungus in nurseries and prevent disease spread.
This document describes a study on the biological control of bacterial onion diseases using the bacterium Pantoea agglomerans strain 2066-7. The study involved screening 77 microorganisms isolated from different plant and environmental sources for antagonistic activity against four bacterial onion pathogens (Pseudomonas marginalis, Pseudomonas viridiflava, Xanthomonas retroflexus, and Pantoea ananatis). Pantoea agglomerans strain 2066-7 showed the strongest antagonistic activity against all four pathogens in vitro. Further experiments found that strain 2066-7 provided complete control of P. marginalis and P. viridiflava at a concentration of 107 CFU/ml,
Efficacy of bacterial bioagents in the management of plant diseasesShikha Sharma
This document summarizes the use of bacterial biocontrol agents for managing plant diseases. It discusses three important bacterial species - Bacillus subtilis, Pseudomonas fluorescens, and Pseudomonas aeruginosa. For each species, it provides the taxonomic classification and describes their morphological characteristics. It also outlines their modes of action, which includes production of antibiotics, siderophores, and inducing systemic resistance in plants. Several studies demonstrating the effectiveness of these bacteria in reducing plant diseases are summarized through tables and figures. The document concludes that bacterial biocontrol agents can offer an ecofriendly and cost-effective strategy for plant disease management.
This document discusses insecticide resistance in insects. It begins by defining insecticide resistance as the ability of some insects to survive exposure to an insecticide that would normally kill them. The document then discusses the history of reported insecticide resistance dating back to 1914. It notes that over 500 insect species have now developed resistance. The mechanisms of resistance include metabolic resistance, target site resistance, behavioral resistance, and penetration resistance. Factors that influence the development of resistance include the frequency of insecticide application and the intensity of selection pressure from the insecticide.
This study aims to evaluate the efficacy of extracts from Origanum Vulgare (oregano) as a mosquito repellent. Specifically, it seeks to determine how long the repellency lasts, compare its effectiveness to synthetic repellents, and rate its efficacy on a scale of 1-10. The study hypothesizes that the repellency will last 30 minutes to 1 hour depending on the amount of extract used, that the organic repellent will be more effective than synthetic options, and that it will rate an 8 on the effectiveness scale. If conducted, the study would use an experimental design to test these hypotheses and help identify a safe and effective natural mosquito repellent.
Two research articles on pesticides in cambodia 2011 02 28 vs 07 2011Scheewe
Brief discussion of the implication of two articles concerning pesticide use in vegetable production in Cambodia and effects on pesticide resiues in market vegetables.
The Effect of Dipel and Spruzit Biopesticides on Metcalfa pruinosa (Say, 1830...IJEAB
Metcalfa pruinosa (Say) is one of the important harmful insect species of the coastal areas of Eastern Black Sea Region. This insect poses a danger by feeding on the juices of hundreds of plants in the region. This study was designed to create a fight strategy against M.pruinosa, which has posed an intense danger in Artvin - Kemalpaşa in recent years, and the effect of Spruzit Neu and Dipel biopesticides on the nymphs and adults of the insect was investigated, and the applicability of these biopesticides was revealed. The study was conducted in the summer season of 2016 when the nymphs and adults of the insect are abundant in the region. In in vitro conditions, the Spruzit Neu (Pyrethrum) and Dipel DF (Bacillu thuringiensis) biopesticides were sprayed at different doses (DiPel® DF BT 100gr / 100lt, Dipel® DF BT 300gr / 100lt, Dipel® DF BT 500gr / 100lt ve Spruzit® Neu) on the nymphs and adults of the insect. The adults and nymphs were checked with 2-day intervals, and the results were assessed according to the One-Way Variance Analysis (ANOVA) and the Duncan Test. It was determined that the most effective applications for the nymphs were Dipel DF 300gr/100lt and 500gr/100lt. It was also determined that the most effective applications for the adult individuals were Spruzit Neu 600ml/100lt and Dipel DF 500gr/100lt doses. The highest death rates in the nymphs were determined in Spruzit Neu 600ml/100lt dose as 72,5%; and in Dipel DF dose as 80%. These rates were determined in Pyrethrum 600 ml/100lt dose as 78%, and in Dipel DF 500 gr/100lt dose as 75%. As a conclusion, it was determined that both biopesticides are influential on the nymphs and adults of M.pruinosa. However, it was also determined that the fight will be more influential in the nymph period of the insect.
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online.
ABSTRACT- Mosquito-borne diseases have intruded the globe since immemorial time. The present scenario for
commanding the mosquitoes is aimed at application of target and stage-specific, cost-effective and biodegradable
phytoproducts. Plant extracts are safer for non-target organisms including man. Plant based formulations would be more
feasible environmental products with proven potential as insecticide. Therefore, in the present study of larvicidal
activity of biologically active compound Apigenin extracted from leaf of Jatropha gossypifolia against the filarial
vector, Culex quinquefasciatus was studied. Standard WHO protocols with minor modifications was adopted for the
larvicidal bioassay. The active compound Apigenin extracted through ethyl alcohol solvent from the leaf of Jatropha
gossypifolia plant of family Euphorbiaceae was administered for 24h or 96h to the larvae of Culex quinquefasciatus.
Exposure of larvae over 24h to sub-lethal doses (40% and 80% of LC50) of apigenin, significantly (P<0.05) altered the
level of total protein, total free amino acid, glycogen and activity of enzymes acetyl cholinesterase, acid and alkaline
phosphatase activity in whole body tissue of Culex quinquefasciatus larvae. The alterations in all these biochemical
parameters were significantly (P<0.05) time and dose dependent.
Key-words- Jatropha gossypifolia, Euphorbiaceae, Culex quinquefasciatus, biochemical effects, Wuchereria bancrofti
This study investigated the biochemical resistance mechanisms of Jatropha curcas against Lasiodiplodia theobramae, a leaf blight and necrosis pathogen. Biochemical parameters were evaluated in 8 Jatropha accessions before and after inoculation with the pathogen. The study found that resistant accessions activated the synthesis of defensive compounds like phenolics, flavonoids, proteins, photosynthetic pigments, and salicylic acid. They also increased antioxidant enzyme activities. Susceptible accessions showed decreased photosynthetic pigments and increased lipid peroxidation. Principal component analysis separated resistant from susceptible accessions based on their biochemical parameter profiles, indicating resistance is associated with the induction of defensive biomolecules and oxidative enzymes.
A Review on the Antimicrobial Activity of Sesuvium Portulacastrumijtsrd
The document summarizes a study on the antimicrobial activity of Sesuvium portulacastrum, a mangrove plant. The study found that ethanol extracts of S. portulacastrum leaves contained phytochemicals like steroids and showed strong antibacterial activity against pathogens like Staphylococcus aureus and E. coli. Gas chromatography-mass spectrometry identified compounds in the ethanol extract including 22, 23-Dihydrostigmasterol, Benzoic acid, Epicatechin, and Capsaicin that were responsible for the antimicrobial properties. The presence of these phytochemicals supports the potential of S. portulacastrum as a source of antimicrobial agents.
Eco-Friendly Nets & Floating Row Covers Reduce Pest Infestation & Improve Tomato Yields for Smallholder Farmers in Kenya; Gardening Guidebook for Kenya www.scribd.com/doc/239851313 ~ Egerton University ~ For more information, Please see websites below:
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Organic Edible Schoolyards & Gardening with Children =
http://scribd.com/doc/239851214 ~
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Double Food Production from your School Garden with Organic Tech =
http://scribd.com/doc/239851079 ~
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Free School Gardening Art Posters =
http://scribd.com/doc/239851159 ~
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Increase Food Production with Companion Planting in your School Garden =
http://scribd.com/doc/239851159 ~
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Healthy Foods Dramatically Improves Student Academic Success =
http://scribd.com/doc/239851348 ~
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City Chickens for your Organic School Garden =
http://scribd.com/doc/239850440 ~
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Huerto Ecológico, Tecnologías Sostenibles, Agricultura Organica
http://scribd.com/doc/239850233
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Simple Square Foot Gardening for Schools - Teacher Guide =
http://scribd.com/doc/239851110
The Effect of Dried Leaves Extract of Hyptis suaveolens on Various Stages of ...iosrjce
IOSR Journal of Pharmacy and Biological Sciences(IOSR-JPBS) is a double blind peer reviewed International Journal that provides rapid publication (within a month) of articles in all areas of Pharmacy and Biological Science. The journal welcomes publications of high quality papers on theoretical developments and practical applications in Pharmacy and Biological Science. Original research papers, state-of-the-art reviews, and high quality technical notes are invited for publications.
Comparative analysis between traditional aquaponics and reconstructed aquapon...bijceesjournal
The aquaponic system of planting is a method that does not require soil usage. It is a method that only needs water, fish, lava rocks (a substitute for soil), and plants. Aquaponic systems are sustainable and environmentally friendly. Its use not only helps to plant in small spaces but also helps reduce artificial chemical use and minimizes excess water use, as aquaponics consumes 90% less water than soil-based gardening. The study applied a descriptive and experimental design to assess and compare conventional and reconstructed aquaponic methods for reproducing tomatoes. The researchers created an observation checklist to determine the significant factors of the study. The study aims to determine the significant difference between traditional aquaponics and reconstructed aquaponics systems propagating tomatoes in terms of height, weight, girth, and number of fruits. The reconstructed aquaponics system’s higher growth yield results in a much more nourished crop than the traditional aquaponics system. It is superior in its number of fruits, height, weight, and girth measurement. Moreover, the reconstructed aquaponics system is proven to eliminate all the hindrances present in the traditional aquaponics system, which are overcrowding of fish, algae growth, pest problems, contaminated water, and dead fish.
Physiological Selectivity of Agrochemicals to Predatory Mites of Tetranychus ...Agriculture Journal IJOEAR
— The growing of rose (Rosa spp.) in a greenhouse provides favorable conditions for both, the plant and the pest mite Tetranychus urticae Koch (Acari: Tetranychidae), for which chemical control is still used. Consumers' demand has encouraged researches to use less aggressive agricultural practices, making the biological control as a viable option. The objective of the present study was to investigate the physiological selectivity of plant protection products, used on rosebushes for the control of T. urticae and other pests or diseases, to Phytoseiulus macropilis (Banks) and Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) both predatory mites of T. urticae on rosebush growing in a greenhouse. According to IOBC/WPRS, the residual method of spraying on a glass and leaf surface area was used for the physiological selectivity test of plant protection products for the predatory mites. The obtained results shown that with the exception of the acaricides-insecticide chlorfenapyr all other tested products-fungicides, acaricides and acaricides-insecticide-methiram + pyraclostrobin, thiofanate-methyl, boscalid + kresoxim-methyl, chlorothalonil, propargite, mandipropamid, mefenoxam, difenoconazol, bifenthrin and pyriproxifen, were innocuous (class 1) or only slightly harmful (class 2) to both species. Chlorfenapyr was highly toxic only for N. californicus (class 4), however after five days of its application no toxic residue of the product was detected on the glass surface and so the product has been classified as innocuous (class 1) as well for this predatory mite species after this period.
A pot experiment was conducted to evaluate the nematicidal efficacy of a biocontrol agent, Pseudomonas flourescens for the management of root-knot nematode, Meloidogyneincognita on chickpea (Cicer arietinum L.) cv.‘Avarodhi’ under glasshouse conditions. All the treatments were found to significantly improve the growth and physiological parameters of chickpea and reduction in pathological parameters as compare to untreated inoculated control. The highest improvement was observed in those plants treated with P. flourescens alone. Concomitant and sequential inoculation of P. flourescens with M.incognita also showed significant improvement in growth parameters of chickpea. Least enhancement in growth parameters was observed in those plants inoculated with nematode alone. It may be due to the nematcidal behaviour of P. flourescens against root-knot nematode, M. incognita. Hence, it may be concluded that P. flourescens as biocontrol agent is better substitute against chemical nematicides for the sustainable management of M. incognita and reduce environmental hazards.
Using next generation sequencing to describe epiphytic microbiota associated ...Agriculture Journal IJOEAR
Abstract— Its seems likely that agricultural management as diverse as certified organic and conventional IPM practices would impact the microbiota associated with crop surfaces differently. We sampled organic and conventionally managed apples at multiple time-points in a growing season and characterized the bacterial taxa associated with replicates of each treatment type. Surprisingly, no evidence of significant differences persisting across multiple time-points was observed. Significant differential abundance of certain taxa was documented but when it was, it was primarily associated with a single time-point making it difficult to understand if these observations resulted from an environmental or a treatment effect. Principal component analyses demonstrated that sampling time-point explained more of the differences between bacterial communities than treatment. Description of dominant shared bacterial families for both organic and conventional samples included Oxalobacteraceae, Pseudomonadaceae, Sphingomonadaceae and Enterobacteriaceae.
This chapter discusses the potential use of infochemicals, such as pheromones and kairomones, in integrated pest management programs. Infochemicals play an important role in mediating ecological interactions between organisms and can be exploited for pest monitoring and control. The banana weevil, Cosmopolites sordidus, produces an aggregation pheromone that attracts both sexes and has been identified and synthesized. There is evidence that host plant volatiles and the synthetic pheromone have an additive effect in attracting C. sordidus. The chapter outlines how research on factors influencing the effectiveness of pheromone-baited traps and integrating traps with biological control agents could provide information
Antimicrobial properties and phytochemical analysis of methanolic extracts of...Alexander Decker
This document summarizes a study that investigated the antimicrobial properties and phytochemical analysis of methanolic extracts of Aframomum melegueta seeds and Zingiber officinale rhizomes on fungal diseases of tomato fruit. Key findings include:
1) Extracts of A. melegueta and Z. officinale significantly reduced the growth of fungi (Aspergillus niger, Penicillium digitatum, Helminthosporium solani, and Mucor piriformis) isolated from diseased tomatoes.
2) Z. officinale extract completely inhibited fungal growth at 25% concentration or higher, while A. melegueta
Early blight of potato caused by Alternaria alternata (Fries) Keissler is one of the factors that affect
potato production. Using chemical control to reduce disease severity represent another risk for
agriculture. Biological control, using microorganisms, as well as plant extract and compost are safe
measures that give reliable control. Applying either measure of control reduced disease incidence to less
than 2% during two successive seasons. Microbial spray of diseased potato reduced the disease to various
extents. Trichoderma sp. was the most effective among all tested microorganisms, followed by
Penicillium sp. On the other hand, alcoholic extract of three plants was more effective than their water
extract. However, water extract showed highly significant reduction of disease incidence. Polygonum
gave the highest reduction of disease incidence in both cases. Also water extract of compost greatly
suppressed early blight when applied as spray to potato leaves. Duration of extraction may affect the
ability of compost extract to suppress disease. All results were comparable to that of fungicides.
Utilization of Biological Control Agents against Phytophthora palmivora Causi...JesonNalanganGeroche
Cacao black pod rot, caused by Phytophthora palmivora, is an economically serious problem in all cacao-producing
regions, leading to global yield loss and tree deaths. With the advent of Republic Act 10068, also known as the Organic
Agriculture Act of 2010, the state is searching for and encouraging sustainable agriculture. Hence, this study was
conducted to determine the antagonistic effects of the different biological control agents against P. palmivora and identify
the most effective biological control agents against cacao pod rot. Conducted at a laboratory, the experiment was laid out
in Complete Randomized Design (CRD) and replicated three times with five samples of cacao pods per replication.
Treatments used were as follows; T1- Negative control, T2- P. palmivora, T3- Chemical control, T4- Bacillus subtilis,
T5- Bacillus amyloliquefaciens, T6- Trichoderma harzianum. The in vitro test was done using a dual culture technique,
while the in vivo test was done through a detached pod test. The dual culture test showed that T. harzianum reduced P.
palmivora radial growth to 6.77mm with a percent growth inhibition of 70.54%. Moreover, the detached pod test recorded
no disease incidence, severity, and incubation period in pods treated with T. harzianum. Whereas, the longer incubation
period was noted in pods treated with chemical control at 4.27 days. Moreover, the highest degree of control was recorded
in pods treated with T. harzianum (100%). These findings emphasized the potential of T. harzianum as a sustainable and
effective solution for combating cacao black pod rot and promoting sustainable agriculture.
Seed treatment with Pseudomonas fluorescens was found to be the most effective in minimizing disease incidence and maximizing yield in chickpeas. It recorded the lowest incidence of wilt disease (11.26%) and root rot (1.43%), as well as a high yield of 7.02 q/ha. Seed treatment with the fungicide Tebuconazole also performed well with low disease incidence and a yield of 6.62 q/ha. Maximum germination (95%) and seedling vigor were observed with Benomyl and Tebuconazole treatments. Overall, treatments with bioagents like P. fluorescens or fungicides effectively controlled seed-borne diseases, improved seed quality parameters, and
This document presents a study on the preliminary shelf life and antioxidant potential of gamma irradiated dried oyster mushrooms (Pleurotus ostreatus) stored over 12 months. Total phenolic content, flavonoid content, and DPPH radical scavenging activity were measured in aqueous, ethanol and methanol extracts at 0, 3, 6, and 12 months. Results showed the mushrooms had high total phenolic content, flavonoid content, and antioxidant activity over storage time. Good correlation was found between total phenolic content and antioxidant activity. The study suggests Pleurotus ostreatus is a promising source of natural antioxidants and that low dose gamma irradiation can help extend shelf life and preserve nutrients of mushrooms.
Similar to Monitoring and pest control of fruit flies in Thailand_ new knowledge for integrated pest management (20)
Monitoring and pest control of fruit flies in Thailand_ new knowledge for integrated pest management
1. Examensarbete
Institutionen för ekologi
Monitoring and pest control of Fruit flies
in Thailand: new knowledge for integrated
pest management.
by
Victor Manuel Guamán Sarango
SJÄLVSTÄNDIGT ARBETE,/INDEPENDENT PROJECT, BIOLOGY, D-NIVÅ/LEVEL, 30 HP
HANDLEDARE (SUPERVISOR):BARBARA EKBOM, INST. FÖR EKOLOGI/ DEPT OF ECOLOGY
EXTERN HANDLEDARE (SUPERVISOR): PETER OOI, DIRECTOR FOR ASIAN REGIONAL CENTER (ARC)
EXAMINATOR (EXAMINER): RICCARDO BOMMARCO, INST. FÖR EKOLOGI/DEPT OF ECOLOGY
Examensarbete
2009:15
Uppsala 2009
SLU, Institutionen för ekologi
Box 7044, 750 07 Uppsala
2. 2
SLU, Sveriges Lantbruksuniversitet/Swedish University of Agricultural Sciences
NL-fakulteten, Fakulteten för Naturresurser och Lantbruk/Faculty of Natural
Resources and Agricultural Sciences, Institutionen för Ekologi/Department of Ecology
Författare/Author: Victor Guamán
Arbetets titel/Title of the project: Monitoring and pest control of Fruit flies in Thailand: new
knowledge for integrated pest management.
Titel på svenska/Title in Swedish: Övervakning och bekämpning av fruktflugor i Thailand: ny
kunskap for integrerad skadedjurbekämpning.
Nyckelord/Key words: Fruit flies, Bactrocera dorsalis, Bactrocera cucurbitae, management,
insecticide resistance, diurnal activity, fly traps
Handledare/Supervisor: Barbara Ekbom
Examinator/Examiner: Riccardo Bommarco
Kurstitel/Title of the course: Independent project
Kurskod/Code: SLU-10293
Omfattning på kursen/Extension of course: 30 hp
Nivå och fördjupning på arbetet/Level and depth of project: Avancerad D/Advanced D
Utgivningsort/Place of publishing: Ultuna
Utgivningsår/Publication year: 2009
Program eller utbildning/Program: Agronom Program, mark& växt
3. 3
ABSTRACT
Fruit flies (Tephritidae) are serious pests that cause enormous losses for farmers in many
countries. The frequent use of insecticides in controlling fruit flies in fruits and vegetable has
not resulted in sustainable management of the pest. Problems associated with this complete
reliance on chemical control are many residues of insecticides in crops, health problems for
farmers, contamination of water and soil, insecticide resistance development and decrease in
natural enemy populations. The implementation of control measures that do not imply an
added burden to the environment and/or the farmers is urgent. More knowledge about the
ecology of fruit flies is the basis for finding new and better ways to control this pest. This
study aimed at 1) develop and testing of a modified Steiner trap constructed from used water
bottles, 2) investigating the diurnal activity of adult males of Bactrocera cucurbitae and
Bactrocera dorsalis, 3) monitoring for resistance to commonly used insecticides in B.
cucurbitae and 4) conducting a survey among farmers with a focus on management of fruit
flies. The research was carried out in Kamphaeng Saen, northwestern part Nakhon Pathom
Province, Thailand from July to August 2009. We constructed an efficient trap from recycled
bottles at the cost of 30 US cents (2.06 SEK) that can be used for monitoring and possibly
also pest control. The results from the diurnal activity experiment suggest that B. cucurbitae
and B. dorsalis are active in the morning. B. dorsalis showed an activity peak between 7 and 8
am. The activity for both species was low before noon and during the first hours of the
afternoon. The bioassay gave no indications of development of resistance to the insecticides
tested. Results from farmer surveys revealed that most of farmers have problems with fruit
flies. The amount of insecticides used in the fields is great and the flora of the compounds is
much diversified. We found also that the farmers spray their fields very frequently during a
growing period until approximately one week before harvest and the spraying was carried out
in a big degree during the cool hours of the day. The results also show that more than 50% of
the farmers used protective equipment. I conclude that since the situation about the control of
these pests is unsustainable the need for further and more intensive studies in this topic is
urgent to carry out. But first of all is of priority to work together with farmers in the issue of
the use of environmental friendly control methods to go away from the dependence of
insecticides.
4. 4
SAMMANFATTNING
Fruktflugor (Tephritidae) är allvarliga skadedjur som orsakar enorma förluster för jordbrukare
i många länder. Den frekventa användningen av insekticider i kontrollen av fruktflugor i frukt
och grönsaker har inte resulterat i en hållbar kontroll av dessa skadedjur. Problemen med
detta totala beroende av kemisk bekämpning är rester av bekämpningsmedel i grödor,
hälsoproblem för jordbrukarna, förorening av vatten och mark, insektsmedel resistens
utveckling och minskning av naturliga fienders populationer. Genomförandet av
kontrollåtgärder som inte innebär en extra belastning på miljön och jordbrukarna är
brådskande. Mer kunskap om fruktflugornas ekologi är grunden till att hitta nya och bättre
sätt att bekämpa de här skadegörarna. Denna studie syftar till att 1) utveckla och testa en
modifierad Steiner fälla gjord av använda vatten flaskor, 2) undersökning av dygnsaktivitet
hos vuxna Bactrocera cucurbitae och Bactrocera dorsalis hannar 3) testning för resistens mot
vanliga insektsmedel i B. cucurbitae och 4) intervjua jordbrukare med fokus på bekämpning
av fruktflugor. Studien utfördes i Kamphaeng Saen, nordvästra delen av Nakhon Pathom
Province, Thailand från juli till augusti 2009. Vi byggde en flugfälla utav gamla vattenflaskor
till ett pris av 2.06 SEK (30 US cents). Resultaten från dygnsaktivitets experiment antydde att
B. cucurbitae och B. dorsalis var mycket aktiva på morgonen. B. dorsalis visade en aktivitets
topp mellan klockan 7-8. Aktiviteten för båda arterna var låg på sen-förmiddagen och under
de första timmarna på eftermiddagen. Testen gav inga indikationer i utveckling av resistens
mot insekticider. Resultat från intervjuerna visade att de flesta jordbrukare har problem med
fruktflugor. Mängden bekämpningsmedel som används av jordbrukaren är stor och den floran
av föreningar är mycket diversifierad. Vi fann också att bönderna besprutar sina fält mycket
ofta under en växtperiod och fortsätter till ungefär en vecka före skörd. Besprutningen utförs i
stor utsträckning under de svala timmarna på dagen. Resultaten visar också att mer en 50% av
jordbrukarna använder skyddsutrustning. Jag drar slutsatser att eftersom situationen om
bekämpning av dessa skadegörare är ohållbart är det angeläget att ytterligare och mer
intensiva studier i detta ämne görs. Men framförallt är det viktigt att prioritera att arbeta
tillsammans med lantbrukaren i frågan om tillämpning av miljövänliga bekämpningsmetoder
för att komma borta från beroendet av insekticider.
5. 5
Contents
ABSTRACT...........................................................................................................................3
SAMMANFATTNING ..........................................................................................................4
CHAPTER 1 ..........................................................................................................................7
1. Introduction ....................................................................................................................7
1.1. Background..............................................................................................................7
1.2. Problem statement....................................................................................................8
1.3. Objectives ................................................................................................................8
CHAPTER 2 ..........................................................................................................................9
2. Litterature review............................................................................................................9
2.1. Agriculture in Thailand ............................................................................................9
2.2. Insecticides...............................................................................................................9
2.2.1. Resistance development ..................................................................................10
2.3. Trapping.................................................................................................................11
2.4. Traps......................................................................................................................11
2.5. Fruit flies - biology and life cycle ...........................................................................12
2.4.1 Damage ................................................................................................................13
2.6. Management of fruit flies .......................................................................................13
2.6.1. Chemical control .................................................................................................13
2.6.2. Cultural control...................................................................................................14
2.6.3. Biological control ...............................................................................................14
2.6.4. Genetic control ...................................................................................................15
2.7. Asian Regional Center............................................................................................15
CHAPTER 3 ........................................................................................................................16
3. Material and Methods ...................................................................................................16
3.1. Experimental sites ..................................................................................................16
3.2. Construction of traps ..............................................................................................17
3.3. Diurnal study of fruit flies ......................................................................................18
3.4. Fly identification ....................................................................................................19
3.5. Insecticide bioassay................................................................................................19
3.6. Farmers survey.......................................................................................................20
3.7. Data analyses..........................................................................................................20
3.8. Study period...........................................................................................................20
CHAPTER 4 ........................................................................................................................21
4. Results..........................................................................................................................21
4.1. Construction of modified Steiner traps....................................................................21
4.1.1. Testing of the traps .............................................................................................21
4.2. Diurnal experiment.................................................................................................21
4.3. Bioassay.................................................................................................................23
4.4. Farmer survey ........................................................................................................24
4.4.1 Use of insecticide .................................................................................................25
4.4.2. Effectiveness of applied insecticide .....................................................................26
4.4.3. Application and protective measures....................................................................27
4.4.4 Sanitary measures.................................................................................................28
CHAPTER 5 ........................................................................................................................29
5. Discussion & Conclusions ............................................................................................29
5.1. Discussion..............................................................................................................29
5.2. Conclusions............................................................................................................31
Acknowledgment..................................................................................................................32
7. 7
CHAPTER 1
1. Introduction
1.1. Background
The production of fruit and vegetables in Thailand generate important sources of income.
These crops represent an important part of the gastronomic culture for Thai people. A
constantly growing population, rising of incomes and urbanization levels increase the demand
of fruit and vegetables. To fill the gap of this demand better farming strategies are necessary.
The presences of pests such as fruit flies constitute an obstacle in their production. Fruit flies
belonging to the family Tephritidae (Order: Diptera) are considered a very destructive group
of insects that cause enormous economic losses in agriculture, especially in a wide variety of
fruits, vegetables and flowers (Diamantidis, 2008). The total number of species within this
family exceeds 4,000. Approximately 10% of them are serious pests distributed around the
world in temperate, subtropical and tropical areas (Christenson and Foote, 1960; Weems et
al., 1999; Singh, 2003). In particular, two species belonging to this family are of great
importance in Thailand, namely Melon fly (Bactrocera cucurbitae (Coquillet)) and Oriental
fruit fly (Bactrocera dorsalis). In cucumber (Cucumis sativus L) and bitter gourd (Momordica
charantia) field infestation problems caused by B. cucurbitae are very common in Thailand
(Ramadan and Messing 2003). The last named represents one of the most popular vegetables
from the cucurbit family in this region. The cost of losses due to infestation of fruit flies can
be surprisingly high, there are examples where losses have been up to 100% in cucurbit
species, caused by Melon fly (Bactrocera cucurbitae) (Dhillon et al, 2005). Crop losses in
mango (12-60%), guava (40-90%) and papaya (12-60%) have also been recorded by Allwood
and Leblanc (1997). The damages on crops consist on oviposition stings on the fruit surface,
fruit that drops early but also destruction of the inside of the fruits. This results in
unmarketable crop.
The use of insecticides as the only way to control pests in fruit and vegetables causes
environmental pollution and hygienic problems that represent a risk for people and animals
(Gallo, 2007). In the last four decades the use of synthetic pesticides such as organophosphate
and carbamates in an extensive way has led to the development of insecticide resistance in a
number of pest species (Casida and Quistad, 1998; Claudianos et al., 1999; Hsu et al., 2004)
and in Thailand residues of organophosphate and organochloride and other compounds have
been detected in soil, water and crops (Thapinta and Hudak, 1998). In Thailand there are
concerns about how to control fruit flies in the most efficient way. Insecticides in the form of
pyrethroids, thriazopos have been used on cucurbits crops but the results have not been
satisfactory. Resistance problems due to the overuse of such insecticides and high residues in
the sprayed vegetables are some of the concerns that necessitate some form of management.
Other approaches to pest fruit fly management, such as use of protein baits have been more or
less ineffective because of our limited knowledge of the ecology of the fruit flies (Somta,
2009 Personal information).
Many ecological studies have been carried out on these two species (Jang, 1997; Kuba et al.,
1982; Kuba et al., 1984; Smith, 1989). Despite this, a lot of knowledge is still lacking and it is
indispensable to the understanding of these pests that this knowledge gap be filled. It is urgent
to find more effective and environmental friendly control strategies that guarantee a
sustainable production of fruits and vegetables.
8. 8
1.2. Problem statement
Knowledge is needed that will increase the effectiveness (and safety) in the use of
insecticides, and that gives farmers and pest advisors ideas about how to develop control
schemes for this pest. A majority of studies carried out on Tephritidae are about the sexual
and oviposition behaviour. Daily activity patterns have been studied less intensively and
among the continents Asia has very few such studies (Aluja and Norrbom, 2000). Daily
activity studies in fruit flies where behavioural factors like locomotion and feeding have been
carried out (Miyatake, 1997) and some observations related to this topic are mentioned by
Christenson and Fotte (1960). Diurnal activity of B. dorsalis and B cucurbitae under field
conditions has not been investigated in Thailand. Since insecticide application is the main
control strategy used by farmers it is crucial to know when during the day fruit flies are most
active and therefore most likely to come into contact with the insecticides.
Furthermore, the construction of a trap for monitoring and control purposes by using material
that can easily be acquired at a low cost will be useful for pest control but also for recycling.
The major advantage of using traps is that the farmers have the possibility to monitor the
species and number of fruit flies present in their fields. In addition the traps have the potential
to be used as a primary tool in the control of fruit flies, then by using baited traps with
insecticide and attractant. It is also important to develop an easy method to monitor
insecticide resistance that can be used, for example, by local advisors and/or farmers without
need to have complicated tools that often are difficult to obtain. Such a practical method could
be used at least once a year.
Information from farmers regarding management of fruit flies but also insecticide use
behaviour is indispensable to gather. That will give a better understanding about how serious
the problem is and therefore how we should act. Related farmer surveys have been carried out
by Lar Soe (2007) in Nakhon Pathom. However, it is important to collect additional
information to fill empty spaces as regards the use of insecticides while setting differences
between fields that grow chilli and cucurbit crops, two of the major important crops in this
area.
1.3. Objectives
This work is aimed to 1) develop and test a modified Steiner trap constructed from recycled
1.5 l water bottles, and easily implemented by farmers, 2) record the diurnal activity of adult
males of B. cucurbitae and B. dorsalis in two different fields located in the agriculture zone of
Nakhon Pathom, Thailand, 3) carry out a quick bioassay to monitor resistance to two well-
known insecticides and 4) interview farmers in this area with a focus on insecticide use and
management of fruit flies. All studies were carried out in Kamphaeng Saen, Nakhon Pathom
Province, Thailand from July to August 2009.
9. 9
CHAPTER 2
2. Litterature review
2.1. Agriculture in Thailand
Thailand covers an area of 513 120 square kilometres; 180 259 square kilometres (35%) is
arable land (NSO, 2004). The majority of Thailand´s population is engaged agriculture both
directly and indirectly (Tualananda, 2000). Crops, forestry, fisheries, agriculture service and
processing of agricultural products are the major divisions of the agro-sector. Favourable
climatic conditions and its geographical position make the production of high quality tropical
fruits possible in Thailand (Chomchalow et al., 2008). During economic crises like the one
experienced during 1997 -1998, the agricultural sector has supported the national economy of
Thailand significantly by serving as a source of employment for unemployed people from
urban zones (Tualanda, 2000).
Farming activities can run throughout the year enabling the supply of fresh products in the
market. The major crops grown are rice, maize, cassava, coconuts, sugarcane, soybean,
rubber, and coffee. Fruits such as mango, lychee, longan, pineapple, and durian are important
as are vegetables and ornamentals such as chilli, tomato, asparagus and flowers. Around 70
different kinds of vegetables are produced every year. Chili, with a production of 4 million
tons in 1998 is a vegetable crop of major importance in Thailand (Nath et al., 1999).
2.2. Insecticides
The use of insecticides has changed agriculture worldwide. In Thailand the import of
chemicals in 2003 was 4,6 million tons, a very remarkable increase compared with 1978´s
level; 0.6 million tons. Insecticides, fungicides and herbicides are imported on a major scale
into the country. The use of insecticides is remarkably high in vegetable and fruit plantations
and its use has had a rising trend since 1977 (Figure 1). Consumer demand for uniform and
perfect looking vegetables and fruits contribute to this increase. Organophosphate,
organochlorine and carbamate compounds are the major classes of insecticides used in
Thailand. The appearance of synthetic pyrethroids in Thailand occurred for first time in 1976
but proof of resistance development in e.g diamondback moth appeared very soon
(Rushtapakornchai and Vattanatangum, 1986).
Figure 1. Amount (ton/a.i). of imported insecticides to Thailand. (Source: Department of Agriculture).
10. 10
The application of insecticides is made directly to the crops; therefore traces of such
compounds may remain even after harvest. This can be an obstacle for the exportation of the
products. Although, many of the crops produced are sold through the informal market which
means that small farmers take their products and sell them directly to consumers without any
control of the hazard levels from insecticides. Cases of poisoning show how serious the
problem is, but also traces of pesticides in soil and water revealed the dangerous results of the
overuse of insecticides (Thapinta and Hudak, 1998). Studies on e.g. Malathion, a widely used
insecticide in fruit and vegetables, showed of chromosome damage in animals and
mammalian cells in cultures (Flessel, 1993). Also neuro-toxic effects are believed to be a
result of a high level insecticide exposure (organophosphate, organochlorine and carbamate)
as well as increased risk of Parkinson disease (Alavanja et.al., 2004; Kamel and Hoppin,
2004).
Most of the pesticides used in Thailand are imported. The percent of pesticides used in fruit
and vegetables were in 1995 nearly ¼ of the total use of chemicals compounds (Figure 2). A
quite new insecticide is Fipronil, which is a very toxic insecticide for animals and possible
carcinogen for humans (Kegley, et al., 2009)
Figure 2. Pesticide use in Thailand. (Source: OAE, 1995)
2.2.1. Resistance development
Insecticide resistance is a serious problem in agriculture, ending up with the tolerance and /or
adaptation of insects to insecticides. The selection pressure that the insecticides place on the
insects makes the organisms with a high fitness to develop resistance. The factors that
influence the development of resistance are: speed of reproduction, mutation and migration of
the insects but also persistence and specificity of insecticides, frequency and number of
applications and type of use insecticide (Aldridge 2009).
Choice of compounds has been more or less based on economic factors (cheap and effective
products that ensure best possible control) rather than the negative effects that the use of
pesticides can have. The impact of different insecticides on both humans and the environment
can vary a lot, therefore a list over insecticides used in agriculture is assigned with a value
(environmental quotient) that represent the influence on farm workers and environment has
been produced by Kovach, et al., (2009) (Table 1). The first category represented are the farm
workers, which is based on the time of exposure to insecticides, the effect on consumers and
leaching, two components that have been put together due to the effect on human health by
for example contamination of groundwater and ecological component, which refers to the
chemicals effects on fish, bees, birds, and beneficial arthropods. The insecticides presented in
the table are some of the most commonly used in Thailand. The main purpose with the values
assigned to each insecticide is to guide farmers and other people that work in agriculture, but
11. 11
also consumers, about the impact of pesticides (Kovach, et al., 2009). The significant impact
that insecticides cause on natural enemies is often underestimated but also the influence on
organisms that contribute to e.g. soil´s fertility depend on what kind of compounds are used
(Gallo, 2007).
Table 1. Environmental quotient of insecticides. The lower the value the lower the impact on health and
environment. (Source: Kovach, et al., 2009)
Insecticide Trade name Farm
worker
Consumers
+ leaching
Ecology Farm work +
consumer +
ecological /3
Abamectin Agri-mek 13.8 3.9 86.4 34.7
Acetamiprid Assail 6.9 7.4 72.0 28.7
Carbosulfan Posse 6.9 8.4 126.7 47.3
Cypermethrin Cymbush 13.8 4.9 89.4 36.4
Dichlorvos Vapona 41.4 17.6 100.8 53.3
Emamectin benzoate Proclaim 9.0 4.0 65.0 26.3
Fipronil Regent 60.0 9.0 203.8 90.9
Malathion Cythion 9.0 4.5 58.0 23.8
Rotenone Fertilome 6.9 2.1 78.3 29.4
Triazophos Hostathion 62.1 7.4 37.3 35.6
2.3. Trapping
Trapping is a useful tool that offers a lot of possibilities to the study and control of fruit flies.
The species presented in a determined area can easily be catalogued by determining their
geographic situation, seasonal abundance, host status and monitoring of already established
fruit fly populations (Allwood, 1997). The information collected in traps such as number of
flies and species, is a valuable source for the planning of control programs but also for
quarantine detection. The attractants and lures can be of either synthetic or natural origin and
are used to catch the male insects. The attractants are several; methyl eugenol, cuelure and
trimedlure are the most commonly used. To trap females, food baits based on fermented sugar
are used. Methyl eugenol was used already in early 1900 (Howlett, 1912) and its effectiveness
in attracting e.g. B. dorsalis has been well proven. There is evidence that methyl eugenol is
involved in the mating effectiveness where males that feed on this lure increase mating
success (Shelly and Dewire, 1994). Similar results were found in studies performed on males
that feed on cuelure and trimedlure. However, the results are not as clear as for methyl
eugenol (Shelly et al. 1996; Shelly and Villalobos 1995). Cuelure is widely used to catch B.
cucurbitae and B. tryoni but studies demonstrated that it can attract to more than 150 different
species of the genus Bactrocera (IAEA, 2003). Trimedlure has been show to be very effective
attracting Ceratitis capitata and Ceratitis rosa. Methyl eugenol can attract nearly 100
different species of the genus Bactrocera (IAEA, 2003). The response to lures is low during
periods when adults are sexually inactive as in winter but also age and time of day can be
influencing factors.
2.4. Traps
The devices used to trap fruit flies are several but the most commonly used are the McPhail
trap, Jackson trap, and Steiner trap. The McPhail trap is commonly used with protein baits. A
common combination between kind of trap and attractants that has remained due its efficiency
is the use of Steiner traps baited with Methyl eugenol and cuelure, Jackson traps baited with
trimedlure and McPhail traps baited with a protein attractant (IAEA, 2003).
The Steiner trap consists of a plastic cylinder with an opening at each end, to minimize the
risk of escape wire gouze covers half of the entrances. Through these openings the flies will
12. 12
go inside and feed on the lure. The trap has a hanger to hang the trap out in the field. Inside, a
support to suspend the attractant piece is placed in the middle of the cylinder (Figure 3).
Figure 3. Steiner trap.
Jackson traps is a delta shaped trap made of material that stands the fields conditions (e.g.
waxed cardboard material). Inside the trap there is a hanger to suspend the lure and in the base
a layer of some sticky material cover it, that to retain the catches. On the top of the trap there
is a wire hanger which serves to hang the trap out in the field. The need of extensive
maintenance may be one disadvantage.
McPhail trap is appropriate for monitoring and mass trapping of flies due to the generous
volume of the container and it is used to catch both females and males. The flies go inside the
trap through openings located on the bottom of the trap. Attracted by protein baits the flies
drown in a liquid baited with insecticide. On the top of the trap there is a hanger to hang the
trap from tree branches.
2.5. Fruit flies - biology and life cycle
Fruit flies breed in fruits but also in other living plant tissues as leaves, buds, stems and
flowers. The host ranges of fruit flies can vary from monophagous (e.g. Mediterranean fruit
flies) to highly polyphagous (e.g. Melon flies and Oriental fruit flies). Simplified it can be
said that fruit flies go through four development stages; eggs, larvae (three larval instars),
pupae and adults. The life cycle from egg to adult takes between 14 and 27 days. The duration
of each stage and degree of survival depends on species, host plant and environmental
conditions (Shaw et al., 1967) (Table 2). Adult fruit flies have a diet based on secretion of
plants from leaves, fruits and rotting fruits but also nectar, pollen, bird feces, and honeydew
secreted by other insects (Christenson and Foote, 1960). Protein obtained from for example
honeydew helps fruit flies to reach a normal fertility and stimulates egg production. Studies
on fruit fly mating behaviour revealed that most of flies in tropical and subtropical areas mate
when light intensity decreases at dusk (Bateman, 1979). Although some species belonging to
the genus Bactrocera prefer to mate in the morning and early afternoon (Alwood, 1997).
Oviposition occurs in stings made by other fruit flies or other injures in the skin. Fruit flies
can move long distances within a short time (Bateman, 1979). Exceptional observations made
by Miyahara and Kawai (1979) showed than a species of the genus Bactrocera could move up
to 200 km. During the larvae stage fruit flies can move long distances by jumping, these
movements seem to be in random directions (Christenson and Foote, 1960) and are probably
defence behaviour against insect predators (Fletcher, 1987).
13. 13
Table 2. Biology review of two important fruit flies belonging to genus Bactrocera.
Duration of development stages
(days)
Specie Host
Egg Larvae Pupae Adult Number
of eggs
B.cucurbitae
(Melon fly)
Cucumber,
water melon,
squash, tomato,
beans, peppers,
cowpea and
more than 125
fruit,
vegetables and
some wild
plants.
Temporary host
plants occur
e.g. papaya,
orange, mango
and peach.
Under field
conditions
eggs hatch
after 26 to
28 hours.
Between 4 to 9
days.
Variations
occur
depending of
kind of fruit
and
environmental
conditions.
Pupal stage
can vary
between 7 to
11 days.
Temperature
is a very
important
factor.
Normally
an adult fly
will live
between 1
to 3 months.
There is
prove of
462 days of
life span.
During life
time 1000
eggs a single
female can
lay.
B. dorsalis
(Oriental
fruit fly)
Guava, mango,
papaya,
avocado and
more than 200
fruit and
vegetables.
24 hours
under
summer
conditions;
20 days in
winter.
9 to 35 days;
6 to 7 under
optimal
environment.
10 to 26 days
depending of
temperature.
Longer period
under cool
temperature.
One to three
months.
About one
year under
cool
conditions.
1200 to 1500
eggs per
female.
The size of
the fruits can
influence the
number of
eggs per fruit
- small fruits
fewer eggs.
After Christenson and Foote (1960).
2.4.1 Damage
Fruit flies damage fruits by puncturing and laying eggs under the soft skin in both mature and
green fruits (Hollingsworth and Allwood, 2000). The eggs hatch and feed inside the fruit
causing the fruits to rot (Dhillon, 2005) resulting in unmarketable fruits. Due to the larva’s
three instars the fruits can be totally destroyed (Ye and Liu, 2005). Furthermore, injuries
caused by the larvae may be used as gateways by secondary organisms (e.g. bacteria and
fungi) and contribute to further destruction of the fruit. At maturity, larvae emerge from the
damaged fruit and drop to the ground and pupate in a burrow (4-8 cm) prepared by the pre-
pupa. Infested fruits often drop to the ground prematurely.
2.6. Management of fruit flies
Fruit fly management can be divided in 4 different categories: chemical, cultural, biological
and genetic.
2.6.1. Chemical control
Chemical control is widely used among farmers. The first synthetic chemical insecticide used
to control fruit flies was DDT. Eventually, DDT was replaced by organophosphates. The
application of insecticides is done by spray cover on the entire crop or trees. Insecticides can
14. 14
also be used in a mix with attractants like cuelure and methyl eugenol. This is a technique
called Male Annihilation Technique (MAT) and consists of many bait stations throughout the
field. The mixture (attractant and insecticide) can be applied in traps like Steiner traps but also
in e.g coconut husk block and soaked paper. This method reduces the male proportion in a
population to a low level and therefore mating does not occur. Experience in field
demonstrated that the level of infestation in mango in India decrease to 5% from levels of
infestation between 17% and 66% by using this technique (Verghese et al, 2006).
Insecticides can even be used together with protein baits. This method is very important in the
control of both female and male fruit flies in distinction when using insecticide and attractants
who is specified for male fruit flies. The protein baits consist of hydrolysed protein mixed
with an appropriate insecticide and applied on crop canopy in different places in the field
(spot technique) (Allwood et al., 2001).
2.6.2. Cultural control
Among the techniques that have shown good results the following are the most successful
sanitation measures, growing crops that better can withstand fruit flies attacks, early harvest,
and bagging.
Sanitation measures
The infested fruits should be removed; in particular the fruit on the tree that present signs of
attack should be removed instead of removing fallen fruits on the ground were the larvae have
already left the fruit. In fields where sanitation measures are practice the level of fruit flies
decreases significantly (Verghese et al., 2004).
Resistant crops
The production of crop varieties that are less attractive for fruit flies has shown good effects.
There are some chilli varieties that are classified as non-hosts for fruit flies in Fiji Islands. In
Thailand there are some fruit crops that are not susceptible to fruit fly attacks (Allwood et al.,
2001).
Early harvest
Fruit flies prefer to attack fruits and vegetables depending on the stage of maturity. In some
crops there is the possibility to harvest fruits early to avoid fruit fly infesting.
Bagging
This is a kind of exclusion. A single fruit or a cluster or even a whole tree can be covered by a
bag. The bags prevent fruit flies from infesting the fruits. Often the bag is made of paper but
also cloth can be a material resistant enough. Bags made of old newspaper can be an
economic and effective way to protect the fruits. In Thailand this method is used in particular
in mango orchards (Allwood et al., 2001). Even plant leaves can be an appropriate material
for bagging fruits (e.g. banana).
2.6.3. Biological control
Introduction of parasitoids to infested fields has given good results in management of fruit
flies. The use of biological control to control fruit flies started already in 1902 (Wharton,
1989). There are examples where reductions of infestation have been nearly 95% as the
experiment in Hawaii showed when larve parasitoids belonging to the families Eulophidae,
Braconidae and Chalcididae were introduced (Allwood et al., 2001). Psyttalia fletcheri
(Hymenoptera: Braconidae) is one of the parasitoids that had showed a high parasitism degree
in B. cucurbitae, Fopius arisanus (Sonan) is other promising parasitoid tested in Hawai to
control B. latifrons (Bokonon-Gatan et al., 2007). A biological control can also be conducted
15. 15
via measures that favour the established parasitoids in a kind of conservation of biological
control agents. The biocontrol agents are often reared in different localizations than the place
where they will be released. In Thailand, it is reported that the potential to find Eulophidae
parasitoids that can be used in Hawaii is great, especially in the north region (Ramadan and
Messing, 2003).
Apart from regular parasitoids even birds that feed on infested fruits in the field are very
important for the reduction of fruit fly populations. Sometimes that kind of predation has been
more successful than the control of fruit flies by parasitoids.
2.6.4. Genetic control
The Sterile Insect Technique (STI) is based on the release of sterilised male fruit flies into the
field. Competition between sterile and wild males for females will end up with females
mating with sterile male flies and therefore no offspring will be generated. Radiation is used
to sterilize the flies. This method requires a great amount of sterile flies which should be in
same proportions to the number of the wild flies but also an appropriate rearing of flies that
carry many of the genetic characteristics presented in the population that will be controlled
(Itô et al., 2003).
2.7. Asian Regional Center
This study was done at the request of AVRDC –The World Vegetable Center. It is an
international agricultural research center dedicated to vegetable research and development.
The headquarters is located in Taiwan and they have a large number of regional offices in
different parts of the world. The main purpose of this center is to alleviate poverty and
malnutrition in the developing world through the increased production and consumption of
safe vegetables (AVRDC brochure, 2009). This study was carried out at the research station
of Asia Regional Center, located in Kamphaeng Saen, under the supervision of its director Dr.
Peter A. C. Ooi.
16. 16
CHAPTER 3
3. Material and Methods
3.1. Experimental sites
The study was carried out in Thailand in two farmer production areas around Kamphaeng
Saen district (Figure 4), in Nakhon Pathom Province, located 90 kilometres west of Bangkok.
Nakhon Pathom is a province located northeast of Bangkok. It is neighboured by Suphan Buri
to the north, Nonthaburi to the east, Kanchanburi to the west, Ratchaburi to the south west
and to the south Samut Sakhon. The province is divided into 6 districts; Kamphaeng Saen,
Muang Nakhon Pathom, Nakhon Chaisi, Sam Phram, Bang Len and Don Tum The agriculture
in this zone has a great diversity but fruit and vegetables occupy a main place. The majority of
fruit and vegetables produced in this zone are distributed in the local market and a lesser part
is exported (Lar Soe, 2007). According to Phadungchom (1999), in this province, pesticides
use is around 19-24% of the total costs of the production of vegetables. The other costs are
labour, fertilizer and seeds.
The fields studied were located 540 metres from each other. The coordinates for field one and
field two are, N 13o
56.278’ E 099o
58.245’ and N 13o
55.910’ E 099o
58.577’, respectively.
Figure 4. Map of Thailand (left) and Nakhon Pathom province (right). Kamphaeng Saen district is highlighted
in yellow.
17. 17
The vegetation compositions in the fields studied were similar, a mix of tree fruits and annual
vegetables.
Field 1
- Mango
- Guava
- Star fruit
- Papaya
- Chili
- Sugar cane
- Citron grass
- Acacia trees
Field 2
- Rose apple
- Cucumber
- Papaya
- Banana
- Ivy gourd
- Beans
- Mango
- Surinam cherry
3.2. Construction of traps
Traps were constructed based on the Steiner traps principle using used water bottles (1.5 l).
The tops of the bottles were cut and later inverted into the body. The edge was sealed with
transparent tape. A perforation of the size of a coin (diameter 20 mm) was made in the bottom
of the bottle. A holder for the attractant was placed inside in the middle of the trap body and
yarn to hang the traps in the field was attached on the top side of the trap (Picture 5). The
attractant pieces inside the trap, consisted of a piece of cloth (3 x 2 cm) soaked with 1 ml of
attractant (cue-lure or methyl eugenol). The trap had two openings covered with a piece of
plastic mesh. The plastic mesh was fastened using glue leaving half of the entries open
(Picture 6). Glue was also used to cover and strengthen the holder and the support (metal
hook). A detailed list of the material used in the construction of the traps is presented in Table
3.
Table 3.Material used for the construction of a modified Steiner trap.
Product Description
1.5 l Water bottles
Yarn
Metal hook
Glue
Tape (transparent)
Plastic mesh
1 bottle/trap
20 cm length
-
-
The size of a coin
Figure 5. Construction of fly traps by recycling used water bottles.
18. 18
Figure 6. Model of a modified Steiner trap, constructed by using used water bottles.
3.3. Diurnal study of fruit flies
Traps were baited with two kinds of attractants, namely methyl eugenol (4-allyl-1,2-
dimethoxybenzene-carboxylate) and cue-lure [4-(p-acetoxyphenyl)-2-butanone]. Both attract
males of B. dorsalis and B. cucurbitae, respectively (Vargas et al., 2000). In the field, the
traps were hung from trees close to the field and about 1m above the ground.
The traps were placed in two different fields twice a week to record the activity of fruit flies.
The experiment was carried out during two weeks for each attractant. One trap at a time was
hung and changed after every 60 minutes, in total 8 traps were used during one sampling
opportunity (Figure 7). The experiment started at 6.00 am and lasted until 14.00 pm.
Figure 7. Traps made from used water bottles.
The temperature in Nakhon Prathom during the time of the study were on average 24.2 and
24.4 in July and August, respectively (Appendix A)
19. 19
3.4. Fly identification
All the fly individuals were counted and identified in the laboratory of AVRDC-ARC by
using a pictorial guide
1
from the International Centre for Management of Pest Fruit Flies
(ICMPFF). A second opinion for the identification was given by Dr. Amporn Winotai,
entomologist at Plant Protection Research and Development Office, Department of
Agriculture, Thailand.
3.5. Insecticide bioassay
Traps were hung in the same fields where the diurnal activity experiment was carried out. The
traps were hung in the afternoon and collected next day in the morning. A total of ten traps
were used each time when the collection was carried out. This activity was repeated 4 times.
The materials in this experiment were empty water bottles, pieces of cloth, rubber bands and
bottle caps. The treatments were: no food, only sugar, abamectin, fipronil, abamectin + sugar
and fipronil + sugar (Table 3). The insecticide dose used in this experiment is the highest dose
recommended on the label of the products, namely abamectin 40 ml/20 l water and fipronil 20
ml/20 l water.
In the lab the flies were transferred to different water bottles previously cut at the bottom and
the opening closed with a cap (Figure 8). Ten flies were placed in each bottle for each
treatment. The flies were transferred by using a black blanket around the trap, which is a
technique that makes the flies fly from the dark place (inside the trap) into the light place.The
treatment (Table 4) was put in a bottle cap with a piece of cotton and placed carefully inside
the bottle. The mortality of the flies was observed after 3, 6, 18, 24, 48 and 72 hours.
Table 4. Treatments used in insecticide bioassay to monitor resistance in B. cucurbitae.
Treatment Composition
No food -
Only sugar 1 ml 5% sugar solution
Abamectin 1 ml Abamectin (2µl abamectin + water)
Fipronil 1 ml Fipronil (1 µl Fipronil + water)
Abamectin + sugar 1 ml Abamectin + 1 ml 5 % sugar solution
Fipronil + sugar 1 ml Fipronil + 1 ml 5 % sugar solution
The experiment was carried out under approximately 22°C and a period of light of 12 hours.
1 Pictorial Key to Common Southeast Asian Fruit Flies (Diptera: Tephritidae: Dacinae)
prepared by the International Centre for Management of Pest Fruit Flies, Kuala Lumpur,
Malaysia
20. 20
Figure 8. Arrangement of the insecticide bioassay (left) and a cap prepared with one treatment for the same
bioassay (right).
3.6. Farmers survey
In order to understand the fruit fly management by farmers in Kampaeng Saen district, one of
the six districts of Nakhon Pathom, interviews were carried out. In total 20 farmers were
interviewed, 10 chili producing farmers and 10 cucurbit growing farmers. Both men and
women participated equally in the interviews. All the farmers were selected randomly. Each
interview took an average of one hour and the questionnaires consisted of 18 questions related
to fruit fly management (Appendix B).
No appointment was needed for the interviews and in order to obtain reliable results with
reliable data, farmers were interviewed in their houses or their fields. The idea was to have the
farmers in an environment where they do not feel strange.
3.7. Data analyses
A Repeated Measures Analyses of Variance was used to analyse the data from the insecticide
bioassay. This statistical analysis is suitable when working with the same materials all the
time, which we did in the insecticide bioassay. The analysis of the data obtained from the
diurnal activity experiment was done by using Standard ANOVA, where comparisons of the
different treatments were done using Student-Newman-Keuls test (SNK) (SAS Institute,
2009).
3.8. Study period
The study was carried out during a period of two months (July – August, 2009) under an
internship arrangement between the Swedish University of Agricultural Sciences and the
Asian Regional Center, AVRDC-The World Vegetable Center. The study was partly funded
by the Swedish International Development Cooperation Agency. The duration was a limiting
factor in this study.
21. 21
CHAPTER 4
4. Results
4.1. Construction of modified Steiner traps
The construction of one trap utilizing used water bottles took between 5-10 minutes. All the
bottles were carefully washed before use to avoid contamination risk. The material used was
purchased from different places in Kamphaeng Saen district. The total number of constructed
traps was considerably more than one hundred. An approximation of price for the
construction of one single trap is 30 US cents (2,06SEK).
4.1.1.Testing of the traps
The traps were tested during the experiment intended to record the diurnal activity of fruit
flies but also during the gathering of fruit flies needed to carry out the test to detect insecticide
resistance (See 4.2 & 4.3). The two different attractants were used under different periods.
The study started with cue-lure and lasted the whole month of July, 2009. Methyl eugenol,
was used the following month. The traps were prepared one day in advance and stored at
room temperature until it was time to use them.
The total number of fruit fly captured during the two months of this study in Kampaeng Saen
was 695 for B. cucurbitae and 761 for B. dorsalis. Furthermore, other species were found
namely B. correcta, B. caudata, B. apicalis and B. cilifera (Table 5).
Table 5. Number of fruit flies catches during July and August, 2009 in KPS.
Fruit Fly species
B . dorsalis B. cucurbitae B. apicalis B. caudata B. cilifera B. correcta
Number of
collected flies 761 695 12 65 47 59
4.2. Diurnal experiment
During the first month of this work the Melon fly was studied. In a period of two weeks, four
observations were performed. The two first observations (21 and 23 July) were carried out
from 6 am until 10 am (Figure 10). For the two other observations (29 and 31 July) the time
of collection was from 6until 2 pm (Figure 10). During the two first days of observation a
majority of flies were caught between 6 and 7 am in field 1 (30%) and between 9 and 10 am
in field 2 (39%). Differences between numbers of catches between the fields were observed,
the major variation was registered 9 am.
The two other observations for the same species (Figure 9, below) shows the major percent of
catches between 7 and 8 am (33%) in field 2 and two hours later in field 1 (30%). The activity
decreases with time in both fields and differences in catches were observed between both
fields. The greatest difference was observed at 7 am.
22. 22
Figure 10. Percent of B. cucurbitae attracted by cue-lure in Kamphaeng Saen, Thailand in July 2009. A total of
four observations were carried out. The length of the experiment differs between the observations. Figure 10A
(upper) shows catches from two days of observation during a time of four hours each. Figure 10B (down) shows
percentage of catches from two days observation during a period of study of eight hours.
The data obtained from four collection times carried out in August 2009 for Oriental fruit fly
showed that the major percent of flies were collected between 7 am and 8 am (35% in both
fields). Also during the first hour of monitoring (6-7 am) a high percentage was registered
(20% and 17% for field 1 and field 2, respectively). A lesser variation in the number of
catches between both fields was obtained and the activity followed a pattern where the
number of catches decreases with time (Figure 11).
23. 23
Figure 11. Procent of B. dorsalis attracted by methyl eugenol in Kamphaeng Saen, Thailand in August 2009.
4.3. Bioassay
The flies were exposed to six different treatments. In the treatments with a mixture of
insecticide and sugar noticeable mortality was observed after 6 hours of exposure. Total
mortality was observed for flies treated with Abamectin and sugar after 48 hours and for
Fipronil and sugar 70 hours after of exposure. In the group treated with only a sugar solution
the survival observed was high during the three days of observation, although a decreasing
pattern was noted (Figure 12A). Flies treated with only insecticide showed a total mortality
similar to the flies that did not receive any sugar solution. For the flies treated with only
insecticides total mortality was noted after 50 hours of exposure, although for Fipronil
exposure flies total mortality was noted already after 24 hours (Figure 12B).
A statistical analysis showed significant difference (P<0.0001) between the different
treatments and time. There was no significant difference among the dates when bioassays
were performed (P=0.11). Studies of flies from two different fields were not significantly
different (P=0.07). Using the Student-Newman-Keuls test it was seen that after 3 hours
treatments containing abamectin had significantly higher mortality than all the other
treatments. After 6 hours the treatment where sugar water was provided had significantly
lower mortality than all the other treatments. Abamectin treatments still had the highest
mortality and were significantly different from the control and fipronil treatments. By 18
hours after the start of the experiment the sugar treatment had significantly lower mortality
than all other treatments and there was no difference in mortality among the other treatments.
This pattern continued until the end of the experiment at 72 hours.
24. 24
Figure 12. Results from the insecticide bioassay on Melon fly (Bactrocera cucurbitae) reared from Kamphaeng
Saen, Thailand in July, 2009. Upper figure (12A) show treatments; sugar, sugar and Abamectin and sugar and
Fipronil. Down figure (12B) shows treatments nothing, Abamectin and Fipronil.
4.4. Farmer survey
The crops found in the fields of each group (chili and cucurbit) had in addition to the main
crop, fruit trees and other crops close to their fields. Star fruit (Averrhoa carambola), rose
25. 25
apple (Syzygium aqueum), mango (Mangifera indica), papaya (Carica papaya), guava
(Psidium guajava) are some of them. Furthermore some fields were mixed with yardlong
bean (Vigna unguiculata subsp. sesquipedalis), acacia (Acacia pennata) and sugar cane
(Saccharum sp).
Most of the farmers in this area have problems with fruit flies. Among the chili farmers, only
one farmer (10%) answered that he did not have problems with this kind of pest. The same
results were observed among the farmers that grow cucurbits. Although, the two farmers did
not have any problems with fruit flies, the farmer in the cucurbit field had problems with
other kinds of insects (e.g. whitefly).
The use of insecticides is widespread among the farmers interviewed. It is the most common
way to control fruit flies and other insects in both chili and cucurbit fields. It was unclear
whether the farmer without fruit fly problems in the chili field used insecticides to control
other pests or not. Otherwise, 19 of 20 farmers used insecticides.
The insecticides used by the farmers in the cucurbit fields are cypermethrin, abamectin,
carbonsulfan acetamipril, methomyl, dicrotophos and emamectin benzoate. Among the chili
farmers the insecticides used are; triazophos, carbonsulfan, abamectin, cypermethrin,
dichlorvos and rotenone (Figure 13). Only one farmer interviewed did not answer what kind
of insecticides was used and it was in a cucurbit field. That was because she did not remember
the name of the compound. Some of the farmers change insecticide each time they spray so
they found it difficult to remember the name of the insecticides. On some occasions we had to
look for used bottles of insecticides in the waste bin.
Most of the farmers ask in the local shop what kind of insecticides they need to purchase.
Only one farmer answered that he usually consulted a close relative before he bought an
insecticide.
Figure 13. Percentage of insecticides used by farmers in Kampaeng Saen, Nakhon Prathom.
4.4.1 Use of insecticide
The use of insecticides in the chili fields is more regular than in the cucurbit fields. Weekly
and biweekly uses of insecticides were the common answers. Cucurbit farmers use
insecticides depending on whether they see any sign of attacks or not. One farmer in this
group used traps baited with an attractant and an insecticide. According to him it works under
a period of three months. Another farmer answered that he used insecticides as often as two -
three times a week.
26. 26
Five (50%) farmers in each group answered that they use the same insecticide all the time,
and four (40%) farmers in each group answered that they do not use the same insecticide.
Farmers belonging to the chili group sprayed more during a crop period than the farmers in
the cucurbit fields. Five (50%) chili farmers sprayed between 15 and 20 times during a crop
period. Four (40%) farmers in the same group answered that they sprayed 8 times or more.
Two farmers in the cucurbit field (20%) answered that they sprayed between 15 and 20 times
during a crop period, two farmers (20%) sprayed 5 times, one farmer (10%) sprayed 3 times.
Information is lacking from three farmers because this question is one of those who were not
included from the beginning. Another farmer use baited traps and he considered that the traps
worked for three months. One farmer in each group did not answer because they did not have
problems with fruit flies.
Four (40%) chili farmers answered that they usually sprayed in the morning, three (30%)
farmers usually sprayed either in the morning or evening time. Two (20%) farmers sprayed in
the evening. Cucurbit farmers have a different spraying pattern. Two farmers sprayed in the
evening, one farmer in the morning and one in the afternoon. Another farmer answered that he
sprayed his field anytime of day. One farmer in each group did not answer to this question
because they did have any problem with fruit flies. Data for the remaining three farmers is
lacking because this questions was not included from the beginning. The farmer that used
traps did not need to answer to this question.
Seven (70%) chili farmers used the dose that is recommended by the label on the insecticide
bottles (Table 6). One farmer answered that she used a higher dose. According to her a higher
dose controls the pest more efficiently. The two remaining farmers did not answer which
dosage they used. In the case of the cucurbit farmers, four farmers (40%) used the
recommended dose and the remaining farmers did not give any answer.
Table 6. Dosage recommended for some insecticides purchased in Kamphaeng Saen 2009.
Insecticide (common name) Dose (cc/20liter water)
Cypermethrin
Malathion
Fipronil
Methomyl
Carbonsulfan
Abamectin
5-10
10-30
20
20-35
20-30
30-40
Source: Respective products leaflet.
The majority of the chili farmers (6) knew that their neighbors use the same type of
insecticides that they used to control fruit fly infestations. Two farmers knew that their
neighbors used another kind of insecticides. Among cucurbit farmers, four of them knew that
their neighbors used the same insecticide. The other four farmers answered that they
neighbors did not use the same insecticides.
The question about the time of spraying before harvest showed that most of the farmers (8)
that cultivated chili applied insecticide a week before they harvest the crop. Only one farmer
in this group sprayed 5 days before harvest. Three of the cucurbit farmers sprayed between
one and three days before harvest, one farmer did it 5 days before, four farmers sprayed one
week before and another farmer did it two weeks before harvest.
4.4.2. Effectiveness of applied insecticide
Most of the farmers in both groups responded positively to the question regarding the
effectiveness of insecticides. Seven chili farmers felt satisfied with the results obtained after
27. 27
they sprayed. Two farmers considered that the insecticides were only sometimes effective.
For cucurbit farmers, seven interviewed considered that the insecticides they used were
effective. Two other farmers have experienced that the insecticides worked, but only
sometimes (Table 7)
Table 7. Results about effectiveness in using insecticides.
Alternatives Chili Farmers Cucurbit farmers
Yes 7 7
No - -
Sometimes 2 2
No answer 1 1
4.4.3. Application and protective measures
The application of the insecticides was done by using either knapsack or motor sprayer
(Figure 14). Half of the chili producer farmers sprayed their crops by using motorized
sprayers, two farmers used both knapsack or motorized sprayers and two other used only
knapsack. In the cucurbit fields motor sprayer was used by four farmers, three farmers used
knapsack, one farmer used both knapsack and motorized sprayer. Another farmer used only
traps, according to him it was an easy way to control pests in his field and avoided coming in
contact with insecticides.
Figure 14. Spray equipment used by farmers in Kamphaeng Saen, Nakhon Pathom. On the left is a knapsack and
on the right is a motorized sprayer.
The farmers were asked if they use any protective clothing in connection with use of
insecticides and if so were asked to specify which ones. Most of the farmers answered that
they used adequate protective clothing. Seven farmers (70%) in the chili fields used personal
protection and only two (20%) answered that they did not. Among the cucurbit growing
farmers, five of them (50%) answered that they used protective clothing and three (30%) did
not use any kind of protection. Percentage of protective equipment used among the farmers
that answered positive to this question is reflected in figure 15.
28. 28
Although many farmers answered that they use some kind of protective equipment my
observations found the opposite. Farmers mixed the insecticides in the back yard (where,
moreover, children and domestic animals spend time).
Figure 15. Percent of protective equipment used by farmers in both chili and cucurbit fields.
When the farmers were asked whether they had experienced any health complication due to
the use of insecticides, the majority of them answered that they did not. Eight farmers in each
group said that they have not felt any illness because of insecticides, while one farmer in each
group said that they had experienced headaches and dizziness.
4.4.4 Sanitary measures
Most of farmers did not use any sanitary measure to control fruit flies. Only three chili
farmers removed infested fruit from their fields. In cucurbit fields four farmers applied the
any kind of sanitation measure. A list of the different sanitary measures practiced by some of
the farmers interviewed follows:
- Dry fallen fruit
- Put fallen fruit in other fields
- Bury fallen fruit in the soil
- Feed fishes with fallen fruit
29. 29
CHAPTER 5
5. Discussion & Conclusions
5.1. Discussion
The results from the fruit flies collected during this study suggest that the Melon fly and the
Oriental fruit fly are active in the morning starting at dawn. Furthermore, I found that there
are differences between the two species in number of flies caught and how long they remain
active in the morning. Oriental fruit fly seems be more active between 7 am and 8 am. For
Melon fly the activity time is longer. In both cases activity declines shortly before noon. The
morning activity is due to the search for food after a long period of inactivity during the night
(Christenson and Foote, 1960). Flies visit host and non host plants when searching for food.
The temperature seems to be a regulatory element of this behaviour, since major activity is
observed during early hours. Previous studies demonstrated that temperature is a very strong
factor that regulates, for example, development rates and other population processes in fruit
flies (Bateman, 2006). Another factor that regulates fruit fly activity is rainfall (Christenson
and Foote, 1960). A preliminary suggestion of an appropriate time of day to control fruit flies
in cucurbit and chilli fields may be during the first hours in morning. Nevertheless, if
temperature is the regulatory factor of its activity, late afternoon time would also be possible.
This is something that should be looked into. In further studies environmental factors such as
air temperature, humidity and rainfall should be included in the analysis.
The number of male flies trapped in field two was, on average, greater than in field one.
Nevertheless, the difference was most apparent for the Melon fly. There is a report that the
sex ratio of, for example, adult Oriental fruit flies is 1:1 that means that the total population of
such flies in the studied fields can be determined from the male adult population (He et al.,
2002).
A very important observation from the monitoring of the diurnal activity of Fruit flies is the
risk that flies could escape before the traps were collected, which would lead to a lower
number of observed captured flies. Using traps with insecticide and an attractant could be part
of the solution, although flies that come in contact with the insecticides and escape will fall to
the ground and disappear. Experiments where different insecticides have been tested to avoid
this problem showing that the choice of insecticide used in the traps is important to solve this
problem (Hill, 1986).
The bioassay results indicated no signs in development of resistance to the insecticides tested.
Nevertheless, it is a preliminary result and should be look into. The lack of a control
population makes the test incomplete. Furthermore, it is possible that there is insecticide
resistance for lower doses of insecticides; therefore a test including different dose levels is
important in this study. The test suggests that when flies fed on sugar solution treated with the
respective insecticide, there are indications that they take up to 48 hours for complete kill.
Fipronil, however, is a quite new insecticide not used in the fields where the flies were
caught. Abamectin, on the other hand, was used to a bigger degree in both cucurbit and chili
fields.
This method seems to be suitable for farmers to check resistance in their fields without need
of advanced tools. It is, however, very important to work with a high number of flies to be
able to find resistance levels below 10% (Roush and Miller, 1986). Because of the wide use of
insecticides it is important to check for insecticide resistance at least once a year. Mortality
among flies treated with only insecticides and the flies that did not receive any kind of food
showed similar rates of survival. Without food, flies survived less than two days at room
30. 30
temperature and this can be compared with earlier studies carried out by Christenson and
Foote (1960) who found that fruit flies can survive at maximum of three days. The lack of
food is devastating for fruit flies, which means that a control of fruit flies by taking away
possible source of food by applying sanitary measures plays an important role in its survival.
Farmers in Kamphaeng Saen face problems with fruit flies. This was confirmed by a majority
of the interviewed farmers. The absence of knowledge about fruit fly biology among farmers
was quite striking. In some cases we had to show fruit flies to them so they would understand
what the topic was about. This is a fact that makes farmers more vulnerable to sale strategies
from chemical companies. A clear example is found in Malaysia, where baited methyl
eugenol is frequently sold in packaging illustrated with pictures of a female melon fly. This is
misleading information due to the fact that attractants do not work on female flies and methyl
eugenol does not work to catch Melon flies but Oriental Fruit flies.
The use of insecticides is widespread among the farmers interviewed. All except one farmer
used insecticides as the main control measure. However, the farmers were satisfied with the
results of using insecticides. Thirteen farmers thought that insecticides were an effective
control measure while four expressed that insecticides worked only sometimes. The
effectiveness of the insecticides could be explained by the exaggerated number of
applications. Many farmers sprayed their fields more than fifteen times during the growing
period without following any quarantine period. Because of this, residues in food will remain
high due to a great number and short interval of applications. Unfortunately, the possibilities
to go away from this exaggerates use of insecticides are limited. For example organic farming
in Thailand is still something very limited due to the absence of serious national certification
but maybe also because of the low awareness of consumers who are not ready to pay higher
prices to compensate the farmers that produce organic crops.
Another explanation can be that the farmers applied the insecticides at the appropriate time.
Most of the farmers spray their fields in the morning. The results from the diurnal activity
experiment suggested that this was the time when studied flies were most active (Figure 10 &
11). Nevertheless, the practice of integrated pest management (IPM) should be something to
strive for because of its effectiveness and gains for the environment and health. Training
farmers to become “IPM experts” is the only way to reach a sustainable production of
vegetables; otherwise the use of pesticides will keep increasing (Ketelaar and Kumat, 2002).
According to Verghese et al. (2004), the practice of IPM to control B. dorsalis can give very
high reductions of infestation in mango fields. Level of reductions between 75% and 100%
are possible if sanitary measures such as the removing of fallen fruit are applied. Fruit left on
the ground serve as important breeding sources (Liquido, 1991). Furthermore, the use of a
single control measure such as insecticides can hardly give a total reduction of fruit flies
infestation since the damage done by larvae in fruit and vegetables is internal, and therefore
difficult to control (Dhillon, 2005).
Farmers in this zone use a great variety of insecticides. It may be a factor that contributes to
lower the risk for development of resistance since it is proved that the use of different
chemicals compounds with different action mechanisms is a good method to minimize risks
for development of resistance.
Most farmers did not experience any health problem due to the use of insecticides. Although,
there is extensive literature that proves how serious the problems related to the exposure of
insecticides are (Conway and Pretty 1991; Flessel, 1993; Kamel and Hoppin, 2004). The use
of adequate protective equipment during the application of insecticides can be part of the
explanation to this result given that as many as twelve farmers actually use some kind of
protective equipment. However, the equipment should be renewed to give the best possible
31. 31
protection otherwise it could turn out to be a harmful factor instead of protective. Further
investigations about possible cocktails, which mean mix of different insecticides at same time,
used by farmers are very important to take into account due to farmers lack knowledge about
synergistic effects.
The truthfulness from farmers when answering the questionnaires is difficult to establish but
some factors that may contribute to a lower frequency of true answers in this study are the
possible problems in communicating with the translator that took care of the interviews, but
also leading questions that get farmers to answer in a certain way. The farmers can also
sometimes make their own scores and answer in the way they think the interviewer want to
hear.
The construction of traps by using old bottles is an economical way to monitor and control
fruit flies but also a recycling strategy. Monitoring for the pest will give a better
understanding of the number and the species present in the field. Nevertheless, development
of injury thresholds for fruit flies are important to determine what control method should be
use but also to establish the magnitude of the efforts. The information on injury threshold for
B. dorsalis and B. cucurbitae is limited because these two pests are considered high risk
quarantine pests. For international trade there is no tolerance. Detection of even 1 larva in a
consignment will result in either rejection or fumigation of the consignment (Hamacek 2009,
Personal information). For domestic production systems in countries where these flies are
endemic it comes down to what are the acceptable losses for the producer. The traps can also
be used as a direct tool in the control of these pests. By using traps baited with insecticide and
an attractant can these pests be control. This is a method known as Male Annihilation
Technique (MAT) which is to minimize the number of male insects. The population of flies
will decrease if there are less available males to mate. The effectiveness of Steiner traps was
demonstrated by Hooper and Drew (1978), where the number of catches in these traps was
superior to the number of flies caught in another kind of traps.
By using traps as a direct tool in the control of fruit flies instead of cover sprays directly on
crops, the reduction of trace of insecticides in the field and in crops are some of the benefits.
Furthermore, the farmers do need to come in contact with insecticides in such a prolonged and
intensified manner as when cover spraying. Apart from its effectiveness, this method is also
very easy to use and perform on trial basis (Mirani, 2007).
The situation in Thailand’s agriculture is definitely unsustainable and must be changed for the
best of the farmers, consumers and the environment. The knowledge of alternative control
methods for fruit flies is quite big and has to be put in practice. Successful results when
practicing different cultural methods like for example sanitary measures show that there are
possibilities to control fruit flies with other tools than insecticides. Of course the solution does
not only lay on this kind of measure but we have to congregate other control methods like for
example biocontrol by releasing of parasitoids but also taking measures to conserve the
already existing natural enemies in the fields. That in conjunct with traps baited with
insecticides can guarantee a moderate use of insecticides. Furthermore, training farmers to
better understand the consequences of using insecticides are an important step in the efforts to
go away from relies on chemicals and also an increasing of stimulant for farmers to start
produce organic food should contribute to a better sustainable farming.
5.2. Conclusions
The conclusions that can be drawn out of this study are the following:
1) The preliminary results suggest Fruit flies to be more active during the morning.
Therefore, for management of both Melon fly and Oriental fruit fly, the control efforts
32. 32
should be made during the time interval 6 am and 10 am. However further and more
intensive studies should be made to validate these results.
2) The modified traps worked very effectively. Nevertheless, it is important to be
attentive to contamination risks in the outer parts of the traps, since small amounts of
attractant on the surface of the trap may disturb the flies and they will not go inside the
trap. The traps have a potential to obtain information on the abundance and species
composition of fruit flies in the field, information that is needed to determine whether
a control measure is needed or not, but traps also have a potential as a tool in the
control of the pest by mass trapping or disruption in mating.
3) The bioassay indicated no signs of development of insecticide resistance.
Nevertheless, it is important to follow this experiment more intensively but also
including other insecticides that according to the farmers interviewed are used more
frequently as for example methomyl, cypermethrin and carbonsulfan. Also tests by
using different dose levels of insecticides are important in testing for resistance.
4) Farmers rely on insecticides to control these two fruit fly species. Therefore,
prolonged studies on the consequences of this exaggerated control method should be
made. Furthermore, it is urgent to train farmers in the use of sustainable control
measures to approach integrated pest management.
Acknowledgment
I would like to express my gratitude to Dr. Peter Aun-Chuan Ooi, Regional Director, Asian
Regional Center of the Asian Vegetable Research and Development Center (AVRDC) for his
guidance and the valuable ideas he came up with during the development of this study.
I also want to express my thanks to the staff at the AVRDC-The World Vegetable Center.
Thanks to Chanida Somta, Sunaree Yoadlers and Tong for your help during the field
activities. Thanks to Pattrawadee Wangprachyanont and Somchit Preongwitayakun for
organizing my stay in Kasetsart University campus. Thank also to the other staff in this center
for the friendly moments we shared.
I also thank to SIDA for the scholarship and my supervisor Barbara Ekbom for her comments
and contribution in this work. I also thanks to Heidi Alto for her help from Sweden during my
field work and finally to my children for enduring my absence while I was in Thailand.
33. 33
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Appendix B
Interview questions
Date Location Parish
1. Do you have any problems with fruit flies in your orchard?
Yes No
2. In which crops you use to have problem with fruit flies?
.........................................................................................
........................................................................................
3. How do you control the fruit flies?
......................................................................
.....................................................................
4. If you use insecticides, what kind of insecticides do you use?
.......................................................................
........................................................................
5. How do you know which insecticide (s) you need to purchase?
..................................................................................................
6. How often do you use the insecticides?
Every 2-3 days weekly monthly
When you see signs of attacks
Other.......................................................
40. 40
7. Do you use the same insecticide(s) all the time?
Yes No
If No, how often you change the insecticide(s)..........................................
8. How many times do you spray your crops (during crop period)?
1 2 3 4 5
Other...............................
9. When in the day do you use to apply the insecticide(s)? ( If possible indicate time)
Morning time .................................................
Afternoon time ................................................
Evening time ..................................................
10. How do you estimate the dose of insecticide?
........................................................................................................................
........................................................................................................................
11. How many days between each spraying?
1 2 3 4 5 6 7
More than 1 week
12. Do you know if your neighbours use the same kind of insecticide that you use?
Yes No
13. Are the insecticides effective?
Yes Sometimes No
14. How do you apply the insecticides?
41. 41
.............................................................................................
.............................................................................................
15. Do you use any protective equipment when spraying your crop?
Yes No
If yes;
Gloves
Appropriate dress
Boots
Face protection
Other....................................
16. Have you experienced any of following symptoms after use of insecticides?
Vomit
Headache
No
Fainting
Eye itch
Nose bleed
“Burns”
Other problems..............................................................................
17. Do you apply any sanitation measures to control fruit flies?
- Remove infested fruits
- Leave infested fruits in the field
If you remove infested fruits, what do you do with them?
- Bury infested fruits in the soil
42. 42
- Feed fishes with infested fruits to in pounds
Other...................................................................
18. How long time before harvest do you applied insecticides to the crops?
1 week 3 weeks 2 weeks
Other..........................
Thank you very much.