An overview of mold toxicity, an increasingly common but under-recognized cause of chronic multi-system illness. This slideshow details the remarkable science underlying the medical understanding of the pathophysiology, diagnosis, and treatment of this condition.
Brief presentation about COVID19 diagnosis ,management and discharge criteria from isolation. Short Discussion about guideline given by Nepal medical council and TUTH for management.
Brief presentation about COVID19 diagnosis ,management and discharge criteria from isolation. Short Discussion about guideline given by Nepal medical council and TUTH for management.
Monkeypox Drug and Vaccine Discovery - Creative BiolabsCreative-Biolabs
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
INTRODUCTION
Toxicology is the science of the poisons. It also studies the nature, effects, detection, assessment and treatment of their effects on biological material.
Toxicology is a multidisciplinary science. The ultimate objective of the combined research is to determine how an organism is affected by exposure to an agent.
This includes an understanding of:
How the agent moves and interact with living cells and tissues of the organism;
What parts of the organism are affected by its presence and health outcomes of this exposure.
Evaluation of the toxicity of substances whose biological effects may not have been well characterized.
The influence of chemical toxicity is mainly
determined by the dosage, duration of exposure,
route of exposure, species, age, sex, and environment.
The goal of toxicology is to contribute to the
general knowledge and harmful actions of
chemical substances.
2. to study their mechanisms of action,
3. and to estimate their possible risks to humans
HISTORY
Dioscorides, a Greek physician in the court of the Roman emperor Nero, made the first attempt to classify plants according to their toxic and therapeutic effect. Poisonous plants and animals were recognized and their extracts used for hunting or in warfare.
In 1500 BC people used hemlock, opium, arrow poisons, and certain metals to poison enemies or for state executions.
Theophrastus Phillipus Auroleus Bombastus von Hohenheim (1493–1541) (also referred to as Paracelsus, a Roman physician from the first century) is considered "the father" of toxicology.
He stated that "All things are poisonous and nothing is without poison; only the dose makes a thing not poisonous.“
Mathieu Orfila (1813) is considered the modern father of toxicology.
In 1850, Jean Stas became the first person to successfully isolate plant poisons from human tissue.
Hippolyte Visart de Bocarmé used nicotine to kill his brother-in-law. He extracted nicotine from tobacco leaves.
The 20th and 21st Centuries have marked by great advancements in the level of understanding of toxicology. DNA and various biochemicals that maintain body functions have been discovered. Our level of knowledge of toxic effects on organs and cells has expanded to the molecular level.
Microbiology Discussion 1 While Gram staining and visualization .docxannandleola
Microbiology Discussion 1
While Gram staining and visualization under a light microscope can be powerful tools to guide a clinical microbiologist in the identification of bacteria, this process rarely, if ever, is sufficient for making a definitive diagnosis of a disease caused by bacteria. On the other hand, electron microscopy is useful for not only assisting virologists in identifying disease-causing viral agents, but may perhaps provide definitive identification of these agents. Hazelton and Gelderblom (2003)1 have made the argument that electron microscopy should be the diagnostic tool of choice in many viral outbreaks because of the rapidity and fidelity of the result.
Do you agree with the statement above or not or not and why? Explain in detail and use the evidence to support your thought.
Discuss the importance of comparing multiple images of the same virus, perhaps from different patients believed to be infected with the same agent.
1Hazelton PR, Gelderblom HR. Electron microscopy for rapid diagnosis of emerging infectious agents.Emerg Infect Dis [serial online] 2003 Mar [date cited]. Available from: URL: http://www.cdc.gov/ncidod/EID/vol9no3/02-0327.htm
Reply back to classmates: Response has to be a paragraph.
1. Yes, i do agree with Hazelton and gelderblom that the electron microscopy should be the diagnostic tool of choice. I agree with this because after reading some articles i have found that the electron microscopy is fast and realiable. When you are trying to identify a disease or viral outbreak, you are going to need something that will give you fast results that you can trust. I also think when using the electron microscopy that you should use another tool to back your findings.
2. I agree with the statement, electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, “open view” of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. Details for efficient sample collection, preparation, and particle enrichment are given. Applications of diagnostic electron microscopy in clinically or epidemiologically critical situations as well as in bioterrorist events are discussed. Electron microscopy can be applied to many body samples and can also hasten routine cell culture diagnosis. To exploit the potential of diagnostic electron microscopy fully, it should be quality controlled, applied as a frontline method, and be coordinated and run in parallel with other diagnostic techniques. This just show that Gram staining is the first step identify a bacteria, when electron microscopy will make a more result to understand where and how the bacteria was produce. I feel that electron microscopy is just a more advance way to diagnosis the reasoning on how a bacteria was caused.
3. I agree with the statement above that the electron micros ...
Why Do People Get Sick in Damp Buildings? The joint conference of the Wisconsin Environmental Health Association and the Bureau of Environmental and Occupational Health (Wisconsin Department of Health Services).
Monkeypox Drug and Vaccine Discovery - Creative BiolabsCreative-Biolabs
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
Recently, we are experiencing rapid globalization of the monkeypox virus in a short time. Monkeypox has infected more than 77,000 people in more than 100 countries worldwide. Mutations have enabled the virus to grow stronger and smarter, evading antiviral drugs and vaccines. To better identify and control the current monkeypox outbreak, efforts to develop drugs and vaccines are critical. This slide presents some important information about monkeypox drug and vaccine discovery.
INTRODUCTION
Toxicology is the science of the poisons. It also studies the nature, effects, detection, assessment and treatment of their effects on biological material.
Toxicology is a multidisciplinary science. The ultimate objective of the combined research is to determine how an organism is affected by exposure to an agent.
This includes an understanding of:
How the agent moves and interact with living cells and tissues of the organism;
What parts of the organism are affected by its presence and health outcomes of this exposure.
Evaluation of the toxicity of substances whose biological effects may not have been well characterized.
The influence of chemical toxicity is mainly
determined by the dosage, duration of exposure,
route of exposure, species, age, sex, and environment.
The goal of toxicology is to contribute to the
general knowledge and harmful actions of
chemical substances.
2. to study their mechanisms of action,
3. and to estimate their possible risks to humans
HISTORY
Dioscorides, a Greek physician in the court of the Roman emperor Nero, made the first attempt to classify plants according to their toxic and therapeutic effect. Poisonous plants and animals were recognized and their extracts used for hunting or in warfare.
In 1500 BC people used hemlock, opium, arrow poisons, and certain metals to poison enemies or for state executions.
Theophrastus Phillipus Auroleus Bombastus von Hohenheim (1493–1541) (also referred to as Paracelsus, a Roman physician from the first century) is considered "the father" of toxicology.
He stated that "All things are poisonous and nothing is without poison; only the dose makes a thing not poisonous.“
Mathieu Orfila (1813) is considered the modern father of toxicology.
In 1850, Jean Stas became the first person to successfully isolate plant poisons from human tissue.
Hippolyte Visart de Bocarmé used nicotine to kill his brother-in-law. He extracted nicotine from tobacco leaves.
The 20th and 21st Centuries have marked by great advancements in the level of understanding of toxicology. DNA and various biochemicals that maintain body functions have been discovered. Our level of knowledge of toxic effects on organs and cells has expanded to the molecular level.
Microbiology Discussion 1 While Gram staining and visualization .docxannandleola
Microbiology Discussion 1
While Gram staining and visualization under a light microscope can be powerful tools to guide a clinical microbiologist in the identification of bacteria, this process rarely, if ever, is sufficient for making a definitive diagnosis of a disease caused by bacteria. On the other hand, electron microscopy is useful for not only assisting virologists in identifying disease-causing viral agents, but may perhaps provide definitive identification of these agents. Hazelton and Gelderblom (2003)1 have made the argument that electron microscopy should be the diagnostic tool of choice in many viral outbreaks because of the rapidity and fidelity of the result.
Do you agree with the statement above or not or not and why? Explain in detail and use the evidence to support your thought.
Discuss the importance of comparing multiple images of the same virus, perhaps from different patients believed to be infected with the same agent.
1Hazelton PR, Gelderblom HR. Electron microscopy for rapid diagnosis of emerging infectious agents.Emerg Infect Dis [serial online] 2003 Mar [date cited]. Available from: URL: http://www.cdc.gov/ncidod/EID/vol9no3/02-0327.htm
Reply back to classmates: Response has to be a paragraph.
1. Yes, i do agree with Hazelton and gelderblom that the electron microscopy should be the diagnostic tool of choice. I agree with this because after reading some articles i have found that the electron microscopy is fast and realiable. When you are trying to identify a disease or viral outbreak, you are going to need something that will give you fast results that you can trust. I also think when using the electron microscopy that you should use another tool to back your findings.
2. I agree with the statement, electron microscopy has two advantages over enzyme-linked immunosorbent assay and nucleic acid amplification tests. After a simple and fast negative stain preparation, the undirected, “open view” of electron microscopy allows rapid morphologic identification and differential diagnosis of different agents contained in the specimen. Details for efficient sample collection, preparation, and particle enrichment are given. Applications of diagnostic electron microscopy in clinically or epidemiologically critical situations as well as in bioterrorist events are discussed. Electron microscopy can be applied to many body samples and can also hasten routine cell culture diagnosis. To exploit the potential of diagnostic electron microscopy fully, it should be quality controlled, applied as a frontline method, and be coordinated and run in parallel with other diagnostic techniques. This just show that Gram staining is the first step identify a bacteria, when electron microscopy will make a more result to understand where and how the bacteria was produce. I feel that electron microscopy is just a more advance way to diagnosis the reasoning on how a bacteria was caused.
3. I agree with the statement above that the electron micros ...
Why Do People Get Sick in Damp Buildings? The joint conference of the Wisconsin Environmental Health Association and the Bureau of Environmental and Occupational Health (Wisconsin Department of Health Services).
Environmental Transmission of Antimicrobial ResistancePranab Chatterjee
This is the second lecture I took for the MPH students at the Indian Institute of Public Health, Delhi, as a part of the Environmental Health module. In this lecture I introduce the students to the basics of AMR and some common modes and routes of transmission of the same through the environment.
CSMT 442 Cost and Estimating 2 Homework 2 `Siteworks MargaritoWhitt221
CSMT 442: Cost and Estimating 2
Homework 2 `Siteworks (20 points) Answer ALL Questions
1. A large stockpile of materials (5400CY) is to be transported to a fill area. The contractor has a rubber-
tired front loader and a number of dump trucks for the job. Based on a study of the job and knowledge of
the operators, time factors for various steps have been determined and are given below along with cost
and capacity factors. Determine the optimum number of dump trucks to assign to the job and duration
and cost of transporting the whole stockpile.
Front loader: cost per hour with operator $70
Total time to load truck 4 minutes
(not including position time)
Truck Cost per hour with driver $60
Positioning time 1 minute
Loading time 4 minutes
Travel to dump 6minutes
Dump time 2 minutes
Return travel 5 minutes
Truck capacity 6 CY
MOS 5425, Advanced Toxicology 1
Course Learning Outcomes for Unit VIII
Upon completion of this unit, students should be able to:
2. Summarize the principles of toxicology.
2.1 Discuss the toxicological effects of a carcinogen.
5. Examine toxic substances that pose a risk to human population and the environment.
5.1 Discuss methods of exposure to carcinogens.
8. Evaluate risk assessment procedures related to chemical carcinogenicity.
8.1 Explain the models for assessing cancer risks.
Course/Unit
Learning Outcomes
Learning Activity
2
Unit Lesson
Chapter 21
2.1
Unit Lesson
Chapter 15
Scholarly Activity
5
Unit Lesson
Chapter 21
5.1
Unit Lesson
Chapter 15
Scholarly Activity
8.1
Chapters 15 and 23
Scholarly Activity
Reading Assignment
Chapter 15: Chemical Carcinogenesis, pp. 259-280
Chapter 21: Epidemiological Issues in Occupational and Environmental Health, pp. 391-394
Chapter 23: Human Health Risk Assessment, pp. 425-440
Unit Lesson
Unit VIII is the second of a two-part discussion on toxic substances and risk assessment. The last unit of this
course covers chemical carcinogens, environmental pollutants, occupational toxicology, and toxicological
evaluation.
Chemical Carcinogens
According to the textbook, cancer is the second leading cause of death in the United States. There are one
million newly diagnosed cases of cancer each year and 1.5 million deaths annually (Roberts, James, &
Williams, 2015). Many factors can contribute to the development of cancer. Genetics, lifestyle choices, and
exposure to environmental factors can all play roles in initiating cancer. Inherited genes at the present time
cannot be controlled, but the latter two factors, lifestyle choices and environmental factors, can be controlled
to a certain extent to reduce the risk of developing cancer. Lifestyle choices include diet, exercise, exposure
UNIT VIII STUDY GUIDE
Chemical Carcinogenesis, Epidemiology,
and Human Health Risk Assessment
...
Review of literature on carbon nanotubes exposure risks to human health, and hypes involved in exageration of risks and raising issues regarding research carried out in unrealistic laboratory environment that exhibit excess of human uptake.
Antibiotic resistance occurs when bacteria change in response to the use of these medicines. A growing number of infections – such as pneumonia, tuberculosis, gonorrhoea, and salmonellosis – are becoming harder to treat as the antibiotics used to treat them become less effective. Antibiotic resistance leads to longer hospital stays,higher medical costs and increased mortality.
Similar to Mold Toxicity - A Chronic Inflammatory Response Syndrome (20)
263778731218 Abortion Clinic /Pills In Harare ,sisternakatoto
263778731218 Abortion Clinic /Pills In Harare ,ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group ABORTION WOMEN’S CLINIC +27730423979 IN women clinic we believe that every woman should be able to make choices in her pregnancy. Our job is to provide compassionate care, safety,affordable and confidential services. That’s why we have won the trust from all generations of women all over the world. we use non surgical method(Abortion pills) to terminate…Dr.LISA +27730423979women Clinic is committed to providing the highest quality of obstetrical and gynecological care to women of all ages. Our dedicated staff aim to treat each patient and her health concerns with compassion and respect.Our dedicated group of receptionists, nurses, and physicians have worked together as a teamof receptionists, nurses, and physicians have worked together as a team wwww.lisywomensclinic.co.za/
Recomendações da OMS sobre cuidados maternos e neonatais para uma experiência pós-natal positiva.
Em consonância com os ODS – Objetivos do Desenvolvimento Sustentável e a Estratégia Global para a Saúde das Mulheres, Crianças e Adolescentes, e aplicando uma abordagem baseada nos direitos humanos, os esforços de cuidados pós-natais devem expandir-se para além da cobertura e da simples sobrevivência, de modo a incluir cuidados de qualidade.
Estas diretrizes visam melhorar a qualidade dos cuidados pós-natais essenciais e de rotina prestados às mulheres e aos recém-nascidos, com o objetivo final de melhorar a saúde e o bem-estar materno e neonatal.
Uma “experiência pós-natal positiva” é um resultado importante para todas as mulheres que dão à luz e para os seus recém-nascidos, estabelecendo as bases para a melhoria da saúde e do bem-estar a curto e longo prazo. Uma experiência pós-natal positiva é definida como aquela em que as mulheres, pessoas que gestam, os recém-nascidos, os casais, os pais, os cuidadores e as famílias recebem informação consistente, garantia e apoio de profissionais de saúde motivados; e onde um sistema de saúde flexível e com recursos reconheça as necessidades das mulheres e dos bebês e respeite o seu contexto cultural.
Estas diretrizes consolidadas apresentam algumas recomendações novas e já bem fundamentadas sobre cuidados pós-natais de rotina para mulheres e neonatos que recebem cuidados no pós-parto em unidades de saúde ou na comunidade, independentemente dos recursos disponíveis.
É fornecido um conjunto abrangente de recomendações para cuidados durante o período puerperal, com ênfase nos cuidados essenciais que todas as mulheres e recém-nascidos devem receber, e com a devida atenção à qualidade dos cuidados; isto é, a entrega e a experiência do cuidado recebido. Estas diretrizes atualizam e ampliam as recomendações da OMS de 2014 sobre cuidados pós-natais da mãe e do recém-nascido e complementam as atuais diretrizes da OMS sobre a gestão de complicações pós-natais.
O estabelecimento da amamentação e o manejo das principais intercorrências é contemplada.
Recomendamos muito.
Vamos discutir essas recomendações no nosso curso de pós-graduação em Aleitamento no Instituto Ciclos.
Esta publicação só está disponível em inglês até o momento.
Prof. Marcus Renato de Carvalho
www.agostodourado.com
Best Ayurvedic medicine for Gas and IndigestionSwastikAyurveda
Here is the updated list of Top Best Ayurvedic medicine for Gas and Indigestion and those are Gas-O-Go Syp for Dyspepsia | Lavizyme Syrup for Acidity | Yumzyme Hepatoprotective Capsules etc
Basavarajeeyam is a Sreshta Sangraha grantha (Compiled book ), written by Neelkanta kotturu Basavaraja Virachita. It contains 25 Prakaranas, First 24 Chapters related to Rogas& 25th to Rasadravyas.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Tom Selleck Health: A Comprehensive Look at the Iconic Actor’s Wellness Journeygreendigital
Tom Selleck, an enduring figure in Hollywood. has captivated audiences for decades with his rugged charm, iconic moustache. and memorable roles in television and film. From his breakout role as Thomas Magnum in Magnum P.I. to his current portrayal of Frank Reagan in Blue Bloods. Selleck's career has spanned over 50 years. But beyond his professional achievements. fans have often been curious about Tom Selleck Health. especially as he has aged in the public eye.
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Introduction
Many have been interested in Tom Selleck health. not only because of his enduring presence on screen but also because of the challenges. and lifestyle choices he has faced and made over the years. This article delves into the various aspects of Tom Selleck health. exploring his fitness regimen, diet, mental health. and the challenges he has encountered as he ages. We'll look at how he maintains his well-being. the health issues he has faced, and his approach to ageing .
Early Life and Career
Childhood and Athletic Beginnings
Tom Selleck was born on January 29, 1945, in Detroit, Michigan, and grew up in Sherman Oaks, California. From an early age, he was involved in sports, particularly basketball. which played a significant role in his physical development. His athletic pursuits continued into college. where he attended the University of Southern California (USC) on a basketball scholarship. This early involvement in sports laid a strong foundation for his physical health and disciplined lifestyle.
Transition to Acting
Selleck's transition from an athlete to an actor came with its physical demands. His first significant role in "Magnum P.I." required him to perform various stunts and maintain a fit appearance. This role, which he played from 1980 to 1988. necessitated a rigorous fitness routine to meet the show's demands. setting the stage for his long-term commitment to health and wellness.
Fitness Regimen
Workout Routine
Tom Selleck health and fitness regimen has evolved. adapting to his changing roles and age. During his "Magnum, P.I." days. Selleck's workouts were intense and focused on building and maintaining muscle mass. His routine included weightlifting, cardiovascular exercises. and specific training for the stunts he performed on the show.
Selleck adjusted his fitness routine as he aged to suit his body's needs. Today, his workouts focus on maintaining flexibility, strength, and cardiovascular health. He incorporates low-impact exercises such as swimming, walking, and light weightlifting. This balanced approach helps him stay fit without putting undue strain on his joints and muscles.
Importance of Flexibility and Mobility
In recent years, Selleck has emphasized the importance of flexibility and mobility in his fitness regimen. Understanding the natural decline in muscle mass and joint flexibility with age. he includes stretching and yoga in his routine. These practices help prevent injuries, improve posture, and maintain mobilit
micro teaching on communication m.sc nursing.pdfAnurag Sharma
Microteaching is a unique model of practice teaching. It is a viable instrument for the. desired change in the teaching behavior or the behavior potential which, in specified types of real. classroom situations, tends to facilitate the achievement of specified types of objectives.
Muktapishti is a traditional Ayurvedic preparation made from Shoditha Mukta (Purified Pearl), is believed to help regulate thyroid function and reduce symptoms of hyperthyroidism due to its cooling and balancing properties. Clinical evidence on its efficacy remains limited, necessitating further research to validate its therapeutic benefits.
2. Chronic Inflammatory Response Syndrome:
CIRS is the adopted name that refers to the underlying pathophysiology of an
increasingly well understood multi-symptom, multi-system illness.
Other Names for CIRS:
•Biotoxin Illness
Refers to the currently understood common denominator
cause: toxins made by living things.
•Mold Toxicity Syndrome
Underplays the importance of inflammagens that combine
with mold toxins to create indoor air capable of making
susceptible people chronically ill. Sometimes called toxic
mold sickness.
•CIRS-WDB
The mold-driven version of CIRS associated with .
Data suggest that 80 percent of human biotoxin illness is driven by
indoor air contaminated with mold toxins and other inflammagens.
Shoemaker RC, Mark L, MacMahon S, Thrasher JD, Grimes C. Research committee report on diagnosis and treatment of chronic
inflammatory response syndrome caused by exposure to the interior environment of water-damaged buildings. Policyholders of America. 2010.
3. Ritchie Shoemaker, MD
• First to link a toxin produced by Pfiesteria
to the Mid-Atlantic outbreaks of human
illness in the late 1990s. (1)
• First to determine that most cases of
biotoxin illness are due to toxins produced
by certain molds known to grow in waterdamaged buildings. (2)
• First to clarify the underlying
pathophysiology of biotoxin illness and to
develop a stepwise protocol to treat it. (3)
1. Shoemaker R, Hudnell K. Possible Estuary-Associated Syndrome: symptoms, vision, and treatment. Environmental Health Perspectives. 2001;109:539-45.
2. Shoemaker R, Hudnell K, House D. Sick Building Syndrome in Water Damaged Buildings: Generalization of the Chronic Biotoxin-Associated
Illness Paradigm to Indoor Toxigenic-Fungi Exposure. 9/2003 5th International Conference on Bioaerosols (conference peer review)
3. Shoemaker R, Hudnell D. A time-series study of sick building syndrome: chronic, biotoxin-associated illness from exposure to water-damaged
buildings. Neurotoxicology and Teratology 2004; 1-18.
4. Stachybotrys: nature’s solution
to the human problem?
• Perhaps the most toxic mold of
them all.
• Requires sustained moisture.
• Makes tricothecene toxins:
• T2 toxin
• Satratoxin H
• T2 toxin allegedly added to the
chemical weapon known as
yellow rain (this is disputed).
• Stachy toxins often found in
heavily water-damaged
buildings.
Stachybotrys chartarum
Copyright Dennis Kunkel / print available thru Astrographics.com
5. Tricothecenes: how toxic are they?
Mechanisms of toxicity
• Cell-cycle arrest
• Mitosis disruption
• Protein synthesis inhibition
• Oxidative cell stress with DNA damage
• Cell membrane disruption and permeability
Stachybotrys micro- and macro-colonies
• Immunostimulatory and immunosuppressive
• Induction of apoptosis and programmed cell death
• Increased expression of inflammatory and apoptotic mRNAs
• Ribotoxic stress response producing MAPK induction of cytokine release
• Endoplasmic reticulum stress with protein misfolding and blocked unfolding
Toxicology Letters, 217(2):149-158.
Deoxynivalinol (DON) is ingested with wheat contaminated by Fusarium mold species.
6. Tricothecenes: brain damage anyone?
Satratoxin H (SH) causes a leaky blood-brain barrier. (1) BBB changes naturally vary depending
on the nature of the situation. (2) A mouse experimental allergic encephalitis (EAE) model studied
the effects of tricothecenes on neuroinflammation. Can SHcause brain interstitial microedema?
•
SH disrupts brain endothelial cells and the
integrity of the blood-brain barrier.
•
SH induces an initial anti-inflammatory
response from astrocytes. Chronic exposure
results in local oxidative stress and neuronal
apoptosis like that seen in lupus, multiple
sclerosis, and Alzheimer’s disease.
•
Results suggest that a chronic immune
response to inhaled SH could produce
neuronal hypersensitivity and damage, which
could be additive with exposure to other
airborne toxins or inflammagens.
1. Karunasena E, Larranaga MD, Simoni JS, Douglas DR, Straus DC. Building-associated neurologic damage modeled in human cells: a
mechanism of neurotoxic effects by exposure to mycotoxins in the indoor environment. Mycopathologia, 2010 Jun 13. PMID:22846391
2. Fabis MJ, Phares TW, Kean RB, Koprowski H, Hooper DC. Blood-brain barrier changes and cell invasion differ between therapeutic
immune clearance or neurotrophic virus and CNS autoimmunity. PNAS. 2008 Oct 7;105(40):15221-15634. (Source of figure above)
7. Sick Building Syndrome (SBS)
•
Modern SBS began after the 1973 OPEC oil embargo prompted builders to
seal the indoor environment off from the outdoors as a way to reduce
energy consumption.
•
This allowed even minor flooding or leaks to foster indoor fungal growth
giving rise to new combinations of toxin-producing indoor molds.
•
Molds release toxin-laden spores in response to contact with inflammatory
man-made building chemicals. Inflammagens from gram-negative bacteria
and mycobacteria can add to the toxic mix.
•
The mold/bacterial/chemical mix can induce a chronic multi-symptom
illness in people who are genetically susceptible to poor toxin clearance.
8. Common Toxin Producing Indoor Molds
Mold species
Toxin types
Aspergillus penicilloides
aflatoxin, ochratoxin
Aspergillus versicolor
chaetosin
Chaetomium globosum
deoxynivalinol, T2 toxin
Penicillium corylophilum
gliotoxin
Stachybotrys chartarum
T2 toxin, satratoxin H
Trichoderma viride
T2 toxin, DAS
Wallemia sebi
walleminol
Penicillium corylophilum
Trichoderma viride
Aspergillus versicolor
Chaetomium globosum
Wallemia sebi
9. Toxic Bioaerosol Dispersal
• Mold toxins are undetectable with conventional
sampling methods due to their unusually small size
(0.001 to 0.005 microns).
Mold
Spores
• Less than 2% of mold toxins are attached to mold
spores (10 to 30 microns). The remainder are
attached to fungal fragments or exist as free-floating
particles. Like other aerosol particles, they are
dispersed by vibration and air turbulence.
• Aerosolized mold toxins mixed with volatile organic
compounds are major threats to indoor air quality
and this warrants collaborative research. (1,2)
Mold
Toxins
1. Rosen J, Heseltine E. WHO guidelines for indoor air quality: Dampness and Mould. Copenhagen:
WHO; 2009:1–228.
2. Indoor mold: better coordination of research on health effects and more consistent guidance would
improve federal efforts. United States Government Accountability Office; 2008:1–60.
10. Toxin-Inflammagen Synergism in the
Air of Water Damaged Buildings
Mold toxins
Homes
Schools
Volatile organic
compounds
Endotoxins
Offices
Actinomycetes
Other
buildings
11. The Environmental Relative
Moldiness Index Test (ERMI)
Recovery from mold toxicity syndrome is likely only if routinely visited
building structures (home, office, and/or school) manifest ERMI scores less
than 2 on a scale from -10 to 20. The ERMI test is currently the only mold
inspection method with predictive value concerning clinical recovery.
ERMI does not account for non-mold inflammagens including VOCs,
endotoxins, actinomycetes, glucans, glycoproteins, and other noxious incitants.
13. Case Criteria for a Diagnosis
of Mold Toxicity Syndrome
1. Multiple unexplained or non-responsive symptoms.
2. Evidence for HLA genetic susceptibility.
3. Evidence for the documented pattern of abnormal
findings seen in exposed HLA-susceptible patients.
• Patient presents with multi-symptom, multi-system illness.
• Past history of exposure to a water damaged building (optional).
• Failed visual contrast sensitivity testing (8% false negative).
• Current ERMI Score >2 in home, office, and/or school.
• Markers for HLA genetic susceptibility (see Rosetta Stone).
• Pattern of abnormal findings seen in biotoxin illness.
14. Visual Contrast Sensitivity Testing
Most biotoxins are neurotoxic. The optic nerve’s superolateral rim carries the
fibers responsible for edge-detection needed for visual contrast sensitivity
(VCS). For vascular supply reasons, this rim is more vulnerable to neurotoxic
effects. Neurotoxicity thus causes a decrease in VCS that self-corrects with a
sufficient reduction in toxin carriage. Age-related decreases in VCS are not
reversible, or so it is assumed.
Biotoxin illness: reversible
Age-related: irreversible?
15. Who gets sick: HLA susceptibility to
poor clearance of biotoxins.
The Rosetta Stone for interpreting HLA nomenclature in biotoxin illness:
DRB1
DQB1
DRB3
DRB4
DRB5
4
3
11/12
3
52B
14
5
53B
7
2/3
13
6
52A,B,C
17
2/3
52A
18
4
52A
15
6
51
16
5
51
11
7
53
53
52B
HLA haplotypes in each row are associated with at least twice the risk of
treatable biotoxin clearance susceptibility problems compared to controls.
Shoemaker R. Linkage disequilibrium in alleles of HLA DR: differential association with susceptibility to chronic illness following exposure
to biologically produced neurotoxins. American Society of Microbiology 2003. (conference peer review).
16. Genes Load the Gun...
• Specific haplotypes affect
susceptibility to poor
clearance of biotoxins.
• More research needed to
clarify relative risks of the
homozygous 8-4 haplotype.
Exposures Pull the Trigger.
• Biotoxin exposures call out
the genetic vulnerability to
poor clearance.
•Molds, blue-green algae,
and dinoflagellates are the
main sources of biotoxin
exposure.
17. Key Biomarker Abnormalities in
Mold Toxicity Syndrome
Biomarkers
Physiology
What it Means
High C4a, Low C3a
Innate immune system in
overdrive due to toxins
A biotoxin burden is present.
High MMP-9
Tissue barrier disruption and
remodeling
Neuroimmune stress and/or
injury is likely.
High TGF beta-1
Immune suppression+activates
Th17-driven inflammation
Imbalanced innate-adaptive
immune response is likely.
Low MSH
Disrupted neuropeptide
Impaired tissue-based immune
regulation of immune function
defenses.
Low ADH
Dysregulation of ACTH and
kidney water conservation
Impaired stress response,
chronic dehydration.
Low or High VEGF
Chronic tissue hypoxia
Imbalanced response to
capillary hypo-perfusion.
Low VIP
Poorly controlled
inflammation and blood flow
Impaired regulation of
multiple functions.
Low T reg cells
Poorly suppressed
inflammation
Chronic inflammatory
response syndrome.
18. References
High C4a,
Low C3a
Zhou W. The new face of anaphylatoxins in immune regulation. Immunobiology. 2012;217(2):225-34 (C3a
up-regulates Th2-driven antibody responses to foreign particles. We await more research into C4aspecific effects on the activation or inhibition of Th1, Th2, Th17, and/or T reg cell activities).
Romi F, Helgeland G, Gilhus NE. Serum levels of matrix metalloproteinases: implications in clinical
High MMP-9 neurology. European Neurology. 2012;67(2):121-8 (MMP-9 levels correlate with multiple sclerosis activity
and ALS neurodegenerative activity).
High TGF
beta-1
Han G, Li F, Singh TP, Wolf P, Wang XJ. The pro-inflammatory role of TGF beta-1: a paradox? International
Journal of Biological Sciences. 2012;8(2):228-35. (TGF beta-1 has both pro-inflammatory [Th17, Th2] and
anti-inflammatory [Foxp3-induced CD4+CD25- T reg cells]).
Low MSH
Catania A, Lonati C, Sordi A, Carlin A, Leonardi P, and Gatti S. The melanocortin system in control of
inflammation. The Scientific World Journal. 2010;10:1840–1853. (MSH and ACTH are melanocortin
peptides. MSH reduces overactive immune responses in various tissues).
Low ADH
Aguilera G, Rabadan-Diehl C. Vasopressinergic regulation of the hypothalamic-pituitary-adrenal axis:
implications for stress adaptation. Regulatory Peptides. 2000;96(1-20;23-9 (Low ADH [vasopressin] levels
reduce stress adaptation capacity by lowering cortisol and kidney water conservation capacity).
Low or High Mayer G. Capillary rarefaction, hypoxia, VEGF, and angiogenesis in chronic renal disease. Nephrology,
Dialysis, and Transplantation. 2011;26(4):1132-7 (If VEGF-induced hypoxia counter-regulatory factors can
VEGF
exert beneficial or harmful effects in chronic kidney disease, they likely do so in other tissues as well).
Low VIP
Low T reg
cells
Shoemaker RS, House D, Ryan JC. Vasoactive intestinal polypeptide (VIP) corrects chronic inflammatory
response syndrome (CIRS) acquired following exposure to water-damaged buildings. Health. 2013;5(3):
396-401 (VIP is a regulator of immune, oxygenation, and blood flow functions).
Goldstein JD, Perol L, Zaragoza B, Baeyens A, Marodon G, Piaggio E. Role of cytokines in thymusderived vs. peripherally derived regulatory T cell differentiation and function. Frontiers in Immunology.
2013;4:155. (TGF-beta can shift activity from T regs to Th17 cells. C3a-C4a-C5a also play a role).
19. CIRS Reduces Levels of Key
Regulatory Neuropeptides
Adrenocorticotrophic hormone
(ACTH)
Pituitary ACTH induces release of
cortisol. Low levels reduce
inflammation control and stress
resilience.
Alpha melanocyte stimulating
hormone (MSH) Pituitary MSH
coordinates mucous membrane-based
immune defenses. Low levels may
result in leaky barriers, low ADH, and
chronic membrane inflammation.
Anti-diuretic hormone (ADH)
Supraoptic Nucleus
source of ADH
Suprachiasmatic Nucleus
source of VIP
Anterior Pituitary
source of ACTH
Hypothalamic ADH induces kidneys
to conserve water. Low levels result in
dehydration and osmolality changes
that aggravate inflammation.
Vasoactive intestinal polypeptide
(VIP)
Down-regulates Th1 responses, upregulates tolerogenic dendritic cells
and antigen-specific T reg cells, and
improves microcirculatory blood flow.
20. Multidrug Resistant Coagulase Negative Staph
(MRCoNS)
Long-considered non-pathogenic for lacking an enzyme that
coagulates plasma, coagulase-negative staph (CoNS) are now
considered pathogens for other reasons, including:
• Their ability to participate in biofilm communities that host
a range of other bacteria and that may also include fungal and
protozoal species.
•Their ability to differentially active gene expression.
•Their ability to make and release hemolysins - pore-forming
toxins that can disrupt cell membranes membranes.
•Their ability to make and release exotoxins that interfere
with host immune defenses that can incapacitate MSH.
•Their ability to develop multidrug resistance (MRCoNS).
If MSH is low, obtain deep
nasal culture for MRCoNS!
Khosravi Y, Ling LC, Loke MF, et al. Determination of biofilm formation capacity of bacterial pathogens associated with ENT
diseases in the Malaysian population. Eur Arch Otorhinolaryngol. 2013 Jul 24.
Zell C, Resch M, Rosenstein R, et al. Characterization of toxin production of coagulase negative staphylococci isolated from food
and starter cultures. Int J Food Microbiol. 2008 Oct 31;127(3):246-51.
21. Rapidly Changing Markers of Biotoxin Exposure
Based on Re-exposure Trials
day 1
C4a
rises
VEGF
rises
day 3
rises
TGFb1
day 2
Leptin
MMP-9
falls
rises
rises
This 3-day Sequential Activation of Innate Immune Elements (SAIIE) panel is a CIRS
health index that answers the question, “Did the building cause the patient’s symptoms
and abnormal biomarkers?” Results correlate well with ERMI results. As a test, SAIIE
must be done in the genetically susceptible who achieved a healthy baseline by toxic
mold avoidance and use of cholestyramine or welchol.
Shoemaker R, House D. Sick building syndrome (SBS) and exposure to water-damaged buildings: Time series
study, clinical trial and mechanisms. Neurotoxicology and Teratology 2006; 573-588.
22. Interpreting C4a Levels
Level
Clinical Significance
2,380-5,000
concerning
5,000-10,000
worrisome
>10,000
needs prompt reduction
>20,000
indicates repeated exposures,
“sicker, quicker” sensitivity,
MASP1 + MASP2 activation
Shoemaker R, House D. Sick building syndrome (SBS) and exposure to water-damaged buildings: Time series
study, clinical trial and mechanisms. Neurotoxicology and Teratology 2006; 573-588.
23. The Roles of C3a and C4a in Host Defenses
The lectin pathway of the complement system serves as a first line of defense against
microbial intruders. The innate immune system recognizes danger signals presented by the
pathogens (pathogen-associated molecular patterns) or altered host cells (damageassociated molecular patterns) by means of germline encoded cell-surface bound or soluble
pattern recognition molecules. These pattern recognition molecules have evolved against
evolutionarily conserved structures of microorganisms, such as carbohydrates and
acetylated compounds.
The pattern recognition molecules do not act alone - they are associated with other proteins,
mainly serine proteases. These serine proteases (MBL-Associated Serine Proteases), or
MASPs, become activated to initiate the complement cascade when the recognition
molecules bind to their target. Activation of the complement cascade culminates in the
destruction and elimination of pathogens or dangerous molecules via direct cell lysis or
opsonization, respectively.
C3a and C4a are anaphylatoxins. C3a reacts to bacterial membrane
molecules. C4a reacts to biotoxins. Lyme disease can raise C3a and
C4a levels. CIRS-WDB raises C4a and tends to lower C3a levels.
Heja D, Kocsis A, Dobo J, et al. Revised mechanism of complement lectin-pathway activation revealing role of
serine protease MASP-1 as the exclusive activator of MASP-2. Proc Natl Acad Sci USA. 2012 Jun 26;109(26):10498-503.
24. How MASP-1 and MASP-2 are activated by the Mannose
Binding Lectin (MBL)-Associated Complement Pathway
Heja D, Kocsis A, Dobo J, et al. Revised mechanism of complement lectin-pathway activation revealing role of
serine protease MASP-1 as the exclusive activator of MASP-2. Proc Natl Acad Sci USA. 2012 Jun 26;109(26):10498-503.
25. The dual regulatory roles of TGF beta-1
Pro-inflammatory Th17 cells vs. Anti-inflammatory T reg cells
Activated Th17 cells supply their own TGFb1 to help maintain their
pro-inflammatory posture in antigen-challenged tissues like
mucous membranes. T reg cell-derived TGFb1 helps maintain the
immune suppressing action of T reg cells in the body’s periphery.
Th17 cells secrete cytokines IL-17, IL-21, and IL-22, shown to be
more active in autoimmune and inflammatory disease.
T reg cells delete autoreactive T cells, induce tolerance, and dampen
inflammation. Natural T regs are thymus-derived. Inducible T regs
are present in the periphery. TGFb1 recruits both types.
Peterson RA. Regulatory T cells: diverse phenotypes integral to immune homeostasis and suppression.
Toxicologic Pathology. 2012;40(2):186-204.
Hatton RD. TGF-b in Th17 cell development: the truth is out there. Immunity. 2011 Mar 25;34(3):288-90.
Han G, Li F, Singh TP, Wolf P, Wang XJ. The pro-inflammatory role of TGF beta-1: a paradox? International
Journal of Biological Sciences. 2012;8(2):228-35.
26. Physical Effects of High C4a Levels
C4a reduces capillary perfusion, producing the following
well-documented pathophysiological effects:
• Reduces VO2 Max and thus lowers anaerobic threshold.
• Lowers ratio of glutamate/glutamine (G/G) as measured by
magnetic resonance spectroscopy (MRS).
• Raises lactate as measured by magnetic resonance spectroscopy.
Neuronal cells synthesize glutamate from glutamine. Glutamate is a substrate for the neuronal TCA cycle.
Thus, in addition to it’s excitatory neurotransmitter role, glutamate is involved in neuronal energy production.
A reduced glutamate/glutamine ratio indicates capillary hypoperfusion, reduced neuronal energy, loss of
glutaminergic excitatory activity, and possibly micro-interstitial edema in the brain. Correction of C4a levels
correlates with improved VO2 max, MRS G/G ratio, MRS lactate, and with improvements in cognitive
function, tolerance for exertion, and other symptoms associated with CIRS.
Shoemaker R, Maizel M. Treatment of elevated C4a in patients with CFS using low doses of erythropoietin safely reduces symptoms and
lowers C4a: a prospective clinical trial 2007, IACFS (conference peer review).
Lanz B, Gruetter R, Joao MN Duarte. Metabolic flux and compartmentation analysis in the brain in vivo. Frontiers in Endocrinology
(Cellular Endocrinology). doi: 10.3389/fendo.2013.00156
27. The role of C4a in Coagulation
In a diagnostic re-exposure trial, Ristocetin-associated factor, von
Willebrand’s (vW) antigen, and multimers of von Willebrand’s
antigen all fell by day 3.
In cases where vW factors initially rose, they included factor VIII,
von Willebrand’s antigen, and ristocetin-associated factor.
Rising levels of C4a prevent the formation of the fibrin multimers
that are needed to form the scaffolding for proper blood clotting.
Without these multimers, mucosal bleeding becomes more likely.
The drug desmopressin (also known as DDAVP) helps correct the
bleeding problem by enhancing the ability of fibrin monomers to
form multimers.
28. Biotoxin Illness is a Cause of Pulmonary Hypertension.
Vasoactive Intestinal Polypeptide can Reverse It.
The pathophysiology of PAH:
Proliferation of pulmonary vascular
endothelium resulting in elevation of
right heart pressures and peripheral
hypoxemia.
Non-invasive diagnosis of PAH:
Stress echo with calculation of right atrial
pressure pre- and post-exertion:
[(tricuspid regurg) x 4 + right atrial pressure]
Treatment of CIRS-PAH with VIP:
Nasally inhaled VIP, 4-6 times daily to
reduce TGFb1-induced airway remodeling.
Shahzad GR, Shabaz MR. Treating pulmonary arterial hypertension: current treatments and future prospects. Therapeutic Advances
in Chronic Disease. 2011 Nov;2(6):359-70.
Shoemaker RS, House D, Ryan JC. Vasoactive intestinal polypeptide (VIP) corrects chronic inflammatory response syndrome
(CIRS) acquired following exposure to water-damaged buildings. Health. 2013;5(3):396-401.
29. Stepwise Treatment Protocol for CIRS
Final Steps
VIP
TGFb1
Correct C4a
Correct VEGF
Middle Steps
Correct MMP-9
Correct ADH/osmolality
Normalize androgen levels
Correct anti-gliadin antibodies
Early Steps
Eradicate multidrug resistant CoNS
Treat with cholestyramine or welchol
Remove from ongoing mold toxin exposure
Shoemaker RS, House D, Ryan JC. Vasoactive intestinal polypeptide (VIP) corrects chronic inflammatory response
syndrome (CIRS) acquired following exposure to water-damaged buildings. Health. 2013;5(3):396-401.
30. Early Treatment Steps for CIRS
Remove from ongoing mold exposure or the deal’s off
Opening
The first step is often the most difficult because recovery is not
possible without strict avoidance of exposure to toxic molds. To get
well one must produce toxin egress while stopping toxin ingress.
31. Early Treatment Steps for CIRS
Treat with cholestyramine (CSM) or Welchol
Patients must adhere to detailed guidelines in order to obtain the best
possible results. Should be monitored by a physician with experience
in the treatment of chronic inflammatory response syndrome.
Shoemaker R, House D. Sick building syndrome (SBS) and exposure to water-damaged buildings: Time series
study, clinical trial and mechanisms. Neurotoxicology and Teratology 2006; 573-588.
32. Early Treatment Steps for CIRS
Test for MRCoNS and eradicate it’s presence
MRCoNS and other biofilm-associated microbes in the deep nasal
space cause various troubles for the immune system and the brain.
Shoemaker R, Hudnell K, House D, Domenico P. Association of nasal carriage of methicillin resistant and multiple antibiotic resistant coagulase
negative staphylococci species with deficiency of alpha melanocyte stimulating hormone in Chronic Fatigue Syndrome: implication for expanded
treatment options. American Society of Microbiology 2003 (conference peer review).
33. Early Treatment Steps for CIRS
Hasten binder-mediated toxin clearance with
a gluten-free, amylose-free diet
The Rosa Parks Bus - Henry Ford Museum
Strict adherence to dietary guidelines leaves more bus seats open for
clearance of toxins. The Welchol bus is slower and smaller than the
CSM bus but it’s more user friendly.
34. Early Treatment Steps for CIRS
Androgen levels tend to self-correct with toxin clearance.
If estradiol is high an aromatase inhibitor may be indicated.
Testosterone replacement causes more problems than it solves.
35. Mid-Treatment Steps for CIRS
If ADH rise lags, consider additional measures.
Drinking more water doesn’t
work because the kidneys
aren’t able to conserve water
when the ADH level is low.
Dehydration alters blood
viscosity, the delivery of
oxygen and nutrition to cells,
and tolerance for exertion.
Replete minerals when osmolality is low.
Use DDAVP (desmopressin) only with close physician supervision.
36. Mid-Treatment Steps for CIRS
If MMP-9 fall lags, consider additional causes for elevation.
Interacting MMPs and TIMPs*
released by varied cell types
in a Breast Ca tumor nodule.
MMPs and TIMPs engage in
ongoing skirmishes in tissue
inflammation zones.
MMP-9 is a gelatinase involved in collagen breakdown and tissue
remodeling processes. If high despite reduced toxin carriage, consider the
possibility of infectious, autoimmune, or neoplastic disease. VIP may
succeed in lowering MMP-9.
*TIMPs = tissue inhibitors of metalloproteinases. Nature Reviews Cancer 2012;2:161-174.
37. Mid-Treatment Steps for CIRS
If VEGF or C4a correction lags,
consider other drivers for abnormal levels.
Ellen Weinstein for the New York Times
Lyme disease and lupus can elevate C4a levels. VEGF can remain high or low
despite treatment when there are separate causes for capillary hypoperfusion.
Shoemaker R, Giclas P, Crowder C, House D. Complement split products C3a and C4a are early markers of acute Lyme disease in tick bite
patients in the United States. International Archives of Allergy Immunol 2008; 146: 255-261.
Stricker RB, Savely VR, Motanya NC, Giclas PC. Complement split products C3a and C4a in chronic Lyme disease. Scandinavian Journal of
Immunology. 2009 Jan;69(1):64-9.
38. Final Treatment Steps for CIRS
If TGFb1 remains high or if symptoms persist despite
normal VCS, clean ERMI, and (-) MRCoNS, treat with nasal VIP.
The molecular
structure of VIP
Vasoactive Intestinal Peptide (VIP) is a neuropeptide, expressed by lymphoid as well as
neural cells, which has diverse effects on the cellular mediators of inflammation and
immunity and is also a potent neurotransmitter. As a neurotransmitter, VIP exerts a potent
bronchodilatory and vasodilatory effect and also induces the house-keeping of mucus
secreted by submucosal glands. It has immunomodulatory functions which include humoral
immune response suppression and inhibition of vascular and bronchial remodeling.
- modified excerpt from Mathioudakis AG, et al. Vasoactive intestinal peptide inhaled agonists;
potential role in respiratory therapeutics. Hippokratia. 2013 Jan-Mar;17(1):12-16.
39. Benefits and Side Effects of VIP
Beneficial Effects of VIP
•
•
•
•
•
•
•
•
Potential Side Effects of VIP
lowers high C4a
• may raise lipase levels
lowers high TGFb1
• may cause stomach or abdominal
raises low VEGF
lowers high VEGF
lowers high MMP-9
raises low vitamin D3
lowers high vitamin D3
raises thymus-derived and
induced T reg cells
• reduces reactivity to WDB
discomfort by reducing gastric
hydrochloric acid secretion
• may cause excessively low blood
pressure (is a vasodilator)
• may cause a rash
For use only with physician
supervision. Stop use of VIP if
lipase rises or if abdominal pain,
hypotension, or rash occur(s).
40. How CIRS-WDB results in a multisymptom,
multisystem illness made worse by usual medical care:
Mold toxins and inflammagens from sick indoor air trigger MBL-related MASP
activation in the genetically susceptible, raising C4a, TGFb1, Leptin, and MMP-9
levels, producing a variety of symptoms.
Inflammatory cytokines disrupt the balance of regulatory neuropeptides, producing
more symptoms. TGFb1 goes higher to suppress immune up-regulation, but balance
may shift from T reg cells to pathogenic Th17 cells, producing more inflammation,
making symptoms worse.
Patients present with a multisymptom, multisystem illness that persists despite
“excellent” medical care from various specialists, at great expense. They have been
told that there is nothing wrong, that “all is explained” by depression, that they need
to get a life or exercise more. Some are discharged from care for being “chronic
complainers” or “high maintenance.” Meanwhile, even the most well-intentioned
specialists are guaranteed to keep missing the data they’re not looking for.
This needs to stop.
41. Estimated scope of the CIRS-WDB problem:
US population: 315 million
One in four = 80 million at risk for CIRS-WDB
NIOSH estimate of percent of US
buildings with water damage: 50% = 40 million exposed to WDB
Conservative estimate of percent
harboring toxic molds: 10% = 4 million with CIRS-WDB
That’s 40 Michigan Wolverine football stadiums
filled with 100,000 people needlessly suffering
from lack of access to competent medical care.
Thomas G, Burton NC, Mueller C, Page E. Comparison of mold exposures, work-related symptoms, and visual contrast sensitivity between
employees at a severely water-damaged school and employees at a school without significant water damage. CDC Workplace Safety and Health:
Health Hazard Evaluation Report. 2010.
Access to competent medical care for people with CIRSWDB needs to be a national health care priority.
42. Calling all health care professionals!
Climb the CIRS-WDB learning curve with the resources below:
All of the above available for purchase through:
www.survivingmold.com