microbial studies on coral mucus
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coral mucus is the method of protection used by coral reef in sea water since it's a species specific a little studies hold on it and there's a lot of research to go on it.
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microbial studies on coral mucus
- 1. Microbial and ecological studies on the mucus of some coral species Presented by: Mariam Elsadek Supervisor: Prof. Mohammed Kotb
- 2. Introduction • Known functions of mucus release are: • Protection against fouling. • Desiccation during air exposure at extreme low tide. • Sedimentation. • Trap particulate matter from the water. • Light-collecting coral surfaces.
- 3. Introduction (cont.) • Mucus is: • coral-derived organic matter. • complex mixture of secreted materials. • species specific composition. (The bacterial composition on the coral mucus depends on the mucus chemical composition which relate to the coral condition).
- 4. Aim of study • To investigate the difference in the bacterial composition of three different coral species at three different depths. • Sources of natural products with interesting biological activities. • Bio-indicator method.
- 5. Materials and methods • Study site: • Southern Egyptian Red sea coast. • Sahl Hasheesh Bay is home to a number of islands and coral reefs with excellent diving and snorkeling.
- 6. Materials and methods (cont.) • Sampling: • Diving to 5, 10 and 15 m. • 3 species (Xenia, Stylophora and Pocillopora). • 50 ml falcon tubes. • Samples collected with its surrounding slime. • Samples kept at ice box for lab. examination.
- 7. Materials and methods (cont.) • Isolation of bacteria: • Media culture preparation.
- 8. Materials and methods (cont.) • Isolation of bacteria: • Sucrose-Sea-Water-agar (SSW). • Thiosulfate-Citrate-Bile- Salts-Sucrose agar (TCBs). • MacConkey agar (MAC).
- 9. Materials and methods (cont.) • Isolation of bacteria: • Serial dilution.
- 10. Materials and methods (cont.) • Isolation of bacteria: • Spread-plate method.
- 11. Materials and methods (cont.) • Morphological characterization and identification.
- 12. Materials and methods (cont.) • Purification of Bacterial Isolates: • Bacterial colonies with different morphological characteristics (representative colonies) were removed. • Streaked to a new plate of the same media. • Incubated at 28ºC for 24 h.
- 13. Results • Three thousand two hundred sixty six (CFU/ml) were counted at three different media. • Hundred fifty two representative colonies were recognized. • Comparison of detected bacteria was carried out according to: • Action at different media • Depth • Species
- 14. Results (cont.) Action of media Points of comparison SSW agar TCBs agar MAC agar Bacterial action At all samples Hard coral only At all samples Highest count Stylophora Pocillopora Pocillopora Highest diversity Stylophora Stylophora Pocillopora
- 15. Results (cont.) Action of depth Points of comparison Five-meter Ten-meter Fifteen-meter Bacterial count (CFU/ml) 1240 1273 753 Bacterial diversity 38 43 52
- 16. Results (cont.) Action of species Points of comparison Pocillopora sp. Xenia sp. Stylophora sp. Bacterial action at the three cultured media Showing activity at all No action at TCBs at all depths Showing activity at all Bacterial count (CFU/ml) 1501 208 1557 Bacterial diversity 42 38 53
- 18. Acknowledgment
- 19. Thank you
Editor's Notes
- the composition of coral mucus has been recorded to vary temporally between coral species with water depth and irradiance level. The adhesive mucus has the ability to trap particulate matter from the water and the light-collecting coral surfaces. Mucus and trapped particles are transported over the coral surface by ciliary currents and are released to the water9 at a magnitude such that mucus can dominate suspended matter around reefs10, 11. Our measured
- Coral mucus contains higher concentrations of organic matter, nutrients, and microbes such as viruses, bacteria, archaea, and protozoa.
- Ice box refrigerator concept
- Don’t forget el foil TCBs don’t aautoclave
- Ssw general media , tcbs media for vibrio ident Mac for medical purposes specific media for differentiation of gram bacterial act
- color, margin, size, elevation, and surface texture and shape
- Morphological characterization and identification color. Texture and size , transperancy
- Idea of representative colony Make sure of contamination Example vibrio of Xenia and some samples
- Discussion of each action associated with medias Liah el mediat action we feh not
- The composition of coral mucus has been recorded to vary temporally between coral species with water depth and irradiance level. Biological events, such as algal competition, reproduction, and diseases, as well as changes in environmental variables, including temperature, pH, nutrients, and dissolved organic carbon, generate shifts in the composition, richness, and abundance of coral-associated bacteria.
- Bacterial comp as bioindicator for long or short term impact