Micro propagation in hi-tech horticulture

1. Presentation on
STAGES AND STEPS INVOLVED IN
MICRO PROPAGATION
COURSE NAME: HI-TECH HORTICULTURE
COURSE CODE: EC-35
SUBMITTED BY:
Sweta Pattanaik

2. Introduction
Stages in Micropropagation
Steps in Microprogation
Advantages
Disadvantages

3. INTRODUCTION:
 ‘Micro-propagation has many synonyms - tissues culture, mass propagation,
in-vitro culture, cloning.
 In vitro clonal propagation.
 It is practices of rapidly multiplying stock plant material to produce a large
number of progeny plants, using modern plant tissue culture methods.
 Widely used for orchids, ferns, many interior foliage plants.
 Commercialization of Micro-propagation 1970s-1980s
 Murashige (1974) Broad commercial application

4. Stages of Micropropagation:
 It is complicated process and mainly involves 3 stages (I, II and III). Some others
added two more stages (stage 0 and IV).
Stage I (Establishment of Explants)
Stage 0 (Selection of explants)
Stage II (Shoot multiplication)
Stage III (Rooting)
Stage IV (Acclimatization)

5. Diagrammatic representation of stages of
micropropagation:

6. Steps of Micropropagation :
 In short, steps of micropropagation can be divided into 4 distinct stages.
Selection of suitable mother plant
Proliferation on medium
Transfer of shoot to a rooting medium
Transfer of plant to soil
By adventitious root
Through callus culture
By apical and auxilary bud

7. Contd.

8. ADVANTAGES :
Production of many plants that are clones of each other.
Produce disease free plant.
Increase yield of plants.
Plant material without any danger of sickness.
Genetically identical plants.
Agronomical advantages.

9. DISADVANTAGES :
Labour operating cost.
Protocol not optimize for all species.
Expensive to setup.
Facilities required.
Infected plants sample can produce infected progeny.
Grow small amount of tissue at high cost.