I am a biotechnologist with expertise in cell culture techniques and bioinformatics analysis.

1. DR. HINA KHALID
House No141-A, Kehakshan colony2, Jaranwala road, Faisalabad, Pakistan|Mobile: +923057616453| hinakhalid863@gcuf.edu.pk |
SUMMARY
• Interdisciplinary scientist with skills and experience in tissue culture, molecular biology and Insilico drug designing
• Participated in collaborative projects, resulting in 10 peer-reviewed publications, including 3 first-authored publications
• Self-motivated, problem-solving and collaborative scientist with excellent communication skills
• Looking to contribute to use computational methods to to discover phytochemicals against Corona Virus
TECHNICAL SKILLS
• Animal Handling: Mouse handling, tissue harvest, IV/IP/IM/SC injections
• Cell Biology: Cell culture, Cell assays, MTT assay, Tissue fixation
• Microscopy/Imaging: Confocal microscopy, Cell imaging and analysis, , Histopathology
• Immunology: Primary immune cell isolation and culture, Flow Cytometry (FACs) analysis, FACS sorting,
• Biochemistry: ELISA, Western Blotting, Enzymatic assays
• Molecular biology: RNA/DNA isolation, PCR, RT/qPCR, Transfection, mini prep, Hemocytometer proficiency, Agarose gel elec-
trophoresis
• Genomics: RNA library construction for high throughput sequencing
• Bioinformatics: Insilico drug designing, Phylogenetic analysis, Sequence analysis, Genome-wide screening, Molecular modeling
and Pathway modeling, Immunoinformatic approaches
RESEARCH EXPERIENCE
Government College University, Faisalabad (GCUF)
Doctoral Researcher Associate June 2017 to May 2019
• Led a projects which focused on the Insilico and experimental investigation of the therapeutic potential of synthetic compounds
against Hepatitis C Virus
• Used molecular docking approach to discover synthetic compound that fit target profile
• Evaluated cytotoxicity and antiviral potential of compounds
• Performed Real –Time PCR and western Blotting of compound
• This project led to the submission of 2 publications
Government College University, Faisalabad (GCUF)
Graduate Researcher July 2012 to Sept 2013
• Led a projects focused on the CCl4 induced hepatic injury modulation through nitric oxide synthase
• Developed animal model of acute liver injury
• Evaluated the role of iNOS and constitutive NOS via gene expression analysis
• Performed histology and Liver test as an index of liver damage
• This project led to the submission of 1 publication
EDUCATION
• PhD, Biotechnology, Government College University, Faisalabad, 2021
• MS, Biotechnology, Government College University, Faisalabad, 2013
TEACHING EXPERIENCE
• 2013-17 - Science Instructor in Lahore Grammar School
AWARDS
• Research Associate in HEC funded project
CONFERENCE PRESENTATIONS
• Trainer in 3 Days Hands On workshop On Computational Methods for Drug Discovery. October 11-13-17.
PUBLICATIONS
1. Hussain, B., Khalid, H., Nadeem, S., Sultana, T., and Aslam, S. (2012). Phylogenetic and Chronological Analysis of Proteins Causing
Alzheimer’s, Parkinson’s and Huntington’s Diseases. Int J Bioautomation, 16(3), 165-178.
2. Khalid, H., Masoud, M. S., Qasim, M., and Ashfaq, U. A. (2014). Targeting cancer stem cells in hepatocellular cancer: a review.
European Journal of Oncology, 19(3), 130-143.
3. Khalid, S., Idrees, S., Khalid, H., Hussain, B., and Tiwari, S. (2015). Ab-InitioPrediction of Sequence and Structural Biology of Fish
Muscle Proteins Using Homology Modeling, Phylogeny and Different Computational Approaches. MOJ Proteomics Bioinform 2
(3): 00047. DOI: 10.15406/mojpb. 2015.02. 00047 gi| 185132813, Q8UVF6 and gi| 49901349. These muscle proteins selected
were titin, dystrophin, filamin, myosin, spectrin.
4. Ashfaq, U. A., and Khalid, H. (2017). Mechanism of Hepatitis C Virus Induced Diabetes Mellitus. Critical Reviews™ in Eukaryotic
Gene Expression, 27(4).

2. 5. Masoud, M. S., Ashfaq, U. A., and Khalid, H. (2018). Interferon-Free Regimen for Hepatitis C: Insight and Management. Critical
Reviews™ in Eukaryotic Gene Expression, 28(4).
6. Ashfaq, U. A., and Khalid, H. (2020). CRISPR/CAS9-Mediated Antiviral Activity: A Tool to Combat Viral Infection. Critical Reviews™
in Eukaryotic Gene Expression, 30(1).
7. Shahid, F., Ashfaq, U. A., Javaid, A., and Khalid, H. (2020). Immunoinformatics guided rational design of a next generation multi
epitope based peptide (MEBP) vaccine by exploring Zika virus proteome. Infection, Genetics and Evolution, 80, 104199.
8. Javaid, A., Ashfaq, U., Zafar, Z., Akmal, A., Taj, S., and Khalid, H. (2020). Phytochemical Analysis and Antidiabetic Potential of
Armoracia Rusticana: Pharmacological and Computational Approach. Combinatorial Chemistry High Throughput Screening.
9. Khalid, H., and Ashfaq, U. A. (2020). Exploring HCV genome to construct multi-epitope based subunit vaccine to battle HCV
infection: Immunoinformatics based approach. Journal of Biomedical Informatics: 103498.
10. Khalid, H., et al. (2020). Discovery of novel Hepatitis C virus inhibitor targeting multiple allosteric sites of NS5B polymerase.
Infection, Genetics and Evolution: 104371.
11. Khalid, H., Ashfaq, U. A. (2020). Molecular docking and Pharmacoinformatics studies reveal potential phytochemicals against
HCV NS5B Polymerase. Combinatorial Chemistry High Throughput Screening.
12. Khalid, H., et al., Discovery of Novel HCV NS5B polymerase inhibitor, 2-(3, 4-dimethyl-5, 5-dioxidobenzo [e] pyrazolo [4, 3-c][1,
2] thiazin-2 (4H)-yl)-N-(2-fluorobenzyl) acetamide via molecular docking and experimental approach. Clinical and Experimental
Pharmacology and Physiology, 2021.
POSTER PRESENTATION
Title: *Inhibition of inducible nitric oxide synthase attenuates acute liver injury in mice (Abstract) Khalid, H.,Masoud, M. S., Ashfaq,
U. A., Qasim, M., Rehman 1st International Conference on Applied Chemical, Biological and Agricultural Sciences (ICACBAS 2014)
February 18-20. GC University, Faisalabad, Pakistan.
OTHER SKILLS
Software GraphPad Prism, Microsoft Word, Excel, PowerPoint, and ImageJ, MOE
Languages English: professional proficiency. Urdu: native. Punjabi: conversational.
REFERENCES
Dr. M. Shareef Masoud Email: masoudshareef@gmail.com Associate Professor, GC University Faisalabad, Allama Iqbal Road, Faisal-
abad. Pakistan
Dr. Usman Ali Ashfaq Email: usmancemb@gmail.com Associate Professor, GC University Faisalabad, Allama Iqbal Road, Faisalabad.
Pakistan.