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Cliff Threlkeld, D.O. Central Florida Research
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object],[object Object]
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Typical Flow Cytometer Computer System Detector & Mechanical Power
Cytometer Power PC & Printer Specimen Tube
Injector  Tip Fluorescence signals Focused laser beam ,[object Object],[object Object]
Flow Cytometry PMT  FITC PMT  PE PMT  ECD PMT  PC5 Dichroic Filters Laser  1 2 3 4 Flow cell Bandpass Filters
FS SS How do Abs work? 3 19 ZAP 70 5
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
 
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object],[object Object]
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object]
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object]
 
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object]
A New Diagnostic Test for Lyme Disease ,[object Object],[object Object],[object Object],[object Object]
 

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Lyme disease-antigen-test-flow-cytometry

Editor's Notes

  1. We specifically measure ZAP-70 in CLL cells defined by CD19 and CD5 coexpression. We also measure ZAP-70 in T cells defined by CD3 and CD5 coexpression. Including FS and SS, each cell is defined by 5 parameters.