The document evaluates the performance of the Galileo Echo automated blood bank instrument for pre-transfusion testing at Willis-Knighton Health System, which consists of one main hospital and three satellite facilities. Various tests were performed in parallel on the Galileo Echo and using existing manual methods, including ABO/Rh typing, weak D testing, and antibody screening and identification. The results showed high concordance rates between the Galileo Echo and manual methods, ranging from 95.0% to 100%. The implementation of the Galileo Echo allowed for standardization of pre-transfusion testing across the health system and the potential for more antibody identifications to be performed in-house.
This document discusses the implementation of nucleic acid testing (NAT) in blood banks to improve transfusion safety. It notes that NAT screening can reduce the risk of transmitting infections like HIV and HCV during the "window period" between exposure and detection by standard serological tests. While NAT provides benefits, it also has challenges like high costs, complexity, and potential for false positives. The document reviews various NAT technologies used in blood banks and strategies for sample pooling to make high-throughput screening feasible. It examines the impact of NAT for different viruses based on their virological characteristics and window periods. Overall NAT screening has become a standard practice due to public pressure despite the technical and economic hurdles involved.
The document discusses Dallas County Health and Human Services Laboratory's use of HIV-1 RNA testing to detect acute HIV infections. It introduces their testing algorithm, pooling methods used to reduce costs, experience identifying 26 acute HIV cases using RNA testing, and interesting case studies identified. The RNA assay used can detect HIV infection earlier than standard antibody tests alone.
A rapid library preparation method with custom assay designs for detection of...Thermo Fisher Scientific
Herein, we describe a new research method for library
preparation using the Ion AmpliSeq™ HD Library Kit with
custom assay designs from Ion AmpliSeq HD Panels for
detection of low level variants from liquid biopsy samples. This
method includes incorporation of molecular tags that enable
0.1% Limit of Detection (LOD) in cell free DNA (cfDNA) and
dual barcodes for sample identification. This method is also
applicable to formalin-fixed paraffin embedded (FFPE)
samples. The libraries can be prepared in as little as 3 hours
and are compatible for analysis with the Ion GeneStudio™ S5
system
NAAT testing can detect HIV within 9-11 days of exposure during the acute infection period, when transmission risk is highest. During acute infection, the virus is rapidly making copies of itself. NAAT detects HIV RNA, while standard tests detect antibodies and cannot detect HIV until 2-12 weeks after exposure. For positive NAAT results, a senior worker contacts the client within 72 hours to expedite follow-up care and medical appointment.
This document summarizes a class on detecting, quantifying, and tracking variations of SARS-CoV-2 RNA from COVID-19 samples. It discusses using quantitative RT-PCR (qRT-PCR) to detect and measure viral RNA levels in samples. Sequencing is used to identify variations in the viral genome over time, and online tools like Nextstrain allow viewing the evolution and global transmission of variants. Genotyping assays are also described that can rapidly screen samples for known single nucleotide variations during PCR.
EVE198 Winter2020 Class 8 - COVID RNA DetectionJonathan Eisen
This document summarizes a class on SARS-CoV-2 RNA detection, quantification, and variation. It discusses how qRT-PCR is used to detect and quantify the virus by amplifying and detecting viral RNA. It also covers sequencing to identify variants, how variants evolve over time, and genotyping assays that can screen samples for known single nucleotide variations. Nextstrain and other online tools are presented that use sequencing data to analyze viral phylogenies, track variant distributions globally, and visualize genetic variations across the SARS-CoV-2 genome.
The document evaluates the performance of the Galileo Echo automated blood bank instrument for pre-transfusion testing at Willis-Knighton Health System, which consists of one main hospital and three satellite facilities. Various tests were performed in parallel on the Galileo Echo and using existing manual methods, including ABO/Rh typing, weak D testing, and antibody screening and identification. The results showed high concordance rates between the Galileo Echo and manual methods, ranging from 95.0% to 100%. The implementation of the Galileo Echo allowed for standardization of pre-transfusion testing across the health system and the potential for more antibody identifications to be performed in-house.
This document discusses the implementation of nucleic acid testing (NAT) in blood banks to improve transfusion safety. It notes that NAT screening can reduce the risk of transmitting infections like HIV and HCV during the "window period" between exposure and detection by standard serological tests. While NAT provides benefits, it also has challenges like high costs, complexity, and potential for false positives. The document reviews various NAT technologies used in blood banks and strategies for sample pooling to make high-throughput screening feasible. It examines the impact of NAT for different viruses based on their virological characteristics and window periods. Overall NAT screening has become a standard practice due to public pressure despite the technical and economic hurdles involved.
The document discusses Dallas County Health and Human Services Laboratory's use of HIV-1 RNA testing to detect acute HIV infections. It introduces their testing algorithm, pooling methods used to reduce costs, experience identifying 26 acute HIV cases using RNA testing, and interesting case studies identified. The RNA assay used can detect HIV infection earlier than standard antibody tests alone.
A rapid library preparation method with custom assay designs for detection of...Thermo Fisher Scientific
Herein, we describe a new research method for library
preparation using the Ion AmpliSeq™ HD Library Kit with
custom assay designs from Ion AmpliSeq HD Panels for
detection of low level variants from liquid biopsy samples. This
method includes incorporation of molecular tags that enable
0.1% Limit of Detection (LOD) in cell free DNA (cfDNA) and
dual barcodes for sample identification. This method is also
applicable to formalin-fixed paraffin embedded (FFPE)
samples. The libraries can be prepared in as little as 3 hours
and are compatible for analysis with the Ion GeneStudio™ S5
system
NAAT testing can detect HIV within 9-11 days of exposure during the acute infection period, when transmission risk is highest. During acute infection, the virus is rapidly making copies of itself. NAAT detects HIV RNA, while standard tests detect antibodies and cannot detect HIV until 2-12 weeks after exposure. For positive NAAT results, a senior worker contacts the client within 72 hours to expedite follow-up care and medical appointment.
This document summarizes a class on detecting, quantifying, and tracking variations of SARS-CoV-2 RNA from COVID-19 samples. It discusses using quantitative RT-PCR (qRT-PCR) to detect and measure viral RNA levels in samples. Sequencing is used to identify variations in the viral genome over time, and online tools like Nextstrain allow viewing the evolution and global transmission of variants. Genotyping assays are also described that can rapidly screen samples for known single nucleotide variations during PCR.
EVE198 Winter2020 Class 8 - COVID RNA DetectionJonathan Eisen
This document summarizes a class on SARS-CoV-2 RNA detection, quantification, and variation. It discusses how qRT-PCR is used to detect and quantify the virus by amplifying and detecting viral RNA. It also covers sequencing to identify variants, how variants evolve over time, and genotyping assays that can screen samples for known single nucleotide variations. Nextstrain and other online tools are presented that use sequencing data to analyze viral phylogenies, track variant distributions globally, and visualize genetic variations across the SARS-CoV-2 genome.
Blood screening, quarantine and releaseRafiq Ahmad
The document discusses procedures for screening donated blood for infectious diseases at a regional laboratory and blood bank. It provides details on:
1. The viruses, bacteria, parasites, and prions that are screened for, including HIV, HCV, HBV, HTLV, syphilis, malaria, and vCJD.
2. The screening markers and recommended tests used to detect these infectious agents, such as ELISA, chemiluminescence, and nucleic acid amplification technologies.
3. Protocols for quarantining and properly disposing of reactive blood units based on screening results, as well as archiving donor samples and blood components for further testing and research.
1. The authors developed a quality-controlled two-color real-time PCR method to reliably test FFPE samples for molecular diagnostics.
2. The method uses internal standards and an internal standard mixture to control for interfering substances in FFPE samples and other experimental variations. It also uses two-color probes and pre-amplification to maximize signal from low RNA samples.
3. The authors validated the method by developing and testing reagents for four genes in a lung cancer diagnostic test. Analytical tests showed the method has acceptable accuracy, precision, and sensitivity. Clinical tests on FFPE lung samples demonstrated 93% diagnostic accuracy, similar to tests on fresh samples.
This document describes the development and validation of screening panels using flow cytometry to identify hematolymphoid malignancies. Specifically, it details the development of a 14 antibody, 10 color myeloid/lymphoid screening tube and its validation in identifying lymphoma, leukemia, myelodysplastic syndromes, and other hematologic malignancies using over 1000 patient samples with high accuracy compared to standard diagnostics. It also describes developing a scoring system using this screening tube to help identify myelodysplastic syndromes.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMerck Life Sciences
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMilliporeSigma
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Japan Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 860-page report provides a comprehensive analysis of the Japanese molecular diagnostics market, including major trends, technologies, applications, and key players. It forecasts test volumes and reagent sales for infectious diseases, genetic diseases, cancer, and other applications. The report profiles leading suppliers and identifies over 60 business opportunities in molecular diagnostic instruments, tests, software, and auxiliary products. It also examines market entry barriers, alternative strategies, and concerns for companies operating in this space.
The document discusses using PCR arrays to profile gene expression and epigenetics. PCR arrays allow researchers to analyze expression of up to 84 genes related to a pathway or disease using real-time PCR. They include controls to check for genomic DNA contamination and assay performance. As an example, the document describes how a researcher could use a PCR array to compare gene expression between metastatic and non-metastatic breast tumor samples.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Yes, the results are statistically significant since the confidence interval does not cross 1. The results are also precise since the confidence interval is narrow. There is no information given to determine if there is publication bias.
Innovative Molecular Diagnostic Technologies and Emerging MarketsReportsnReports
This document provides a 1050-page report on innovative molecular diagnostic technologies and emerging markets. It explores business and technology trends in major countries and provides forecasts for market size, test volumes, and shares of leading competitors over the next 5-10 years. The report also profiles leading market players, identifies opportunities in product development, and discusses strategies for companies to successfully capitalize on the growing molecular diagnostics market.
The document summarizes recent updates in tuberculosis (TB) diagnostics and treatment. It discusses improvements and gaps in diagnosing TB infection versus active disease. New World Health Organization recommendations for TB diagnostics are outlined. Challenges in aligning current diagnostics with new multidrug-resistant TB treatment options are described. The future of TB diagnostics includes biomarkers and moving away from reliance on sputum samples for diagnosis. New tests are needed to address diagnostic gaps and better monitor treatment response.
Precision medicine for oncology requires accurate and sensitive molecular characterization. However, sample degradation, polymerase errors, and sequencing errors reduce accuracy for sequencing genetic variants. By incorporating molecular tagged adapters in target enrichment, and using DNA probes that deliver extremely even and deep coverage, we are able to demonstrate a 300-fold reduction in false positives at or above 0.25% variant frequency. In this presentation, Dr Mirna Jarosz discusses these methods and how they can significantly reduce error rates in your sequencing data.
The document discusses standard laboratory practices and standard operating procedures (SOPs) developed by the Health Protection Agency (HPA) in the UK. It provides an overview of the HPA's Standards and Evaluations units, which develop SOPs for clinical microbiology laboratories. It also discusses feedback from a survey of laboratories on the SOP for investigating genital tract specimens, including which aspects they found useful and suggestions for improvement. The talk concludes with plans to develop a new SOP for screening group B streptococcus from genital swabs.
US Molecular Diagnostics Market: Innovative Technologies and Emerging Busines...ReportsnReports
This 880-page report provides an extensive analysis of the US molecular diagnostics market, including forecasts of test volumes and reagent sales for various applications through 2022. It reviews DNA sequencing and probe technologies, detection methods, automated instrumentation, and profiles major suppliers. The report also identifies over 60 business opportunities in instruments, tests, software, and auxiliary products and discusses strategies for market entry.
1) Tuberculosis (TB) is commonly diagnosed through direct microscopy, culture, immunodiagnostic tests, molecular tests, and histopathology using samples from sputum, BAL, CSF, tissues, and other body fluids.
2) Direct microscopy has low sensitivity but is quick, while culture has higher sensitivity and allows drug susceptibility testing but takes 1-2 weeks for results. Newer liquid culture systems can provide results in only a few days.
3) Molecular tests like PCR and interferon-gamma release assays provide rapid results within hours and are also used for diagnosis, but many have high costs.
Italy Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 850-page report provides a comprehensive analysis of the Italian molecular diagnostics market, including major issues, trends, technologies, test categories, suppliers, and business opportunities. It forecasts test volumes and reagent sales for infectious diseases, genetic diseases, cancer, forensic testing, and other areas. The report profiles leading suppliers and identifies over 60 market opportunities. It also discusses alternative market strategies, barriers, and competitive landscapes. An executive summary analyzes the Italian business environment and market structure.
A simple and rapid dna extraction method from FINA nd qPCRManish Thakur
A simple and rapid DNA extraction method from whole blood for highly sensitive detection and quantitation of HIV-1 pro-viral DNA by real-time PCR (Journal of Virological Methods 214 (2015) 37–42 )
Spain Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 860-page report provides a comprehensive analysis of the molecular diagnostics market in Spain, including key trends, technologies, major applications and suppliers. It covers topics such as DNA sequencing, probe technologies, detection methods, instrumentation, emerging applications in oncology, infectious diseases and pharmacogenomics. The report also identifies over 60 specific business opportunities and strategies for companies to enter the Spanish molecular diagnostics market.
Blood screening, quarantine and releaseRafiq Ahmad
The document discusses procedures for screening donated blood for infectious diseases at a regional laboratory and blood bank. It provides details on:
1. The viruses, bacteria, parasites, and prions that are screened for, including HIV, HCV, HBV, HTLV, syphilis, malaria, and vCJD.
2. The screening markers and recommended tests used to detect these infectious agents, such as ELISA, chemiluminescence, and nucleic acid amplification technologies.
3. Protocols for quarantining and properly disposing of reactive blood units based on screening results, as well as archiving donor samples and blood components for further testing and research.
1. The authors developed a quality-controlled two-color real-time PCR method to reliably test FFPE samples for molecular diagnostics.
2. The method uses internal standards and an internal standard mixture to control for interfering substances in FFPE samples and other experimental variations. It also uses two-color probes and pre-amplification to maximize signal from low RNA samples.
3. The authors validated the method by developing and testing reagents for four genes in a lung cancer diagnostic test. Analytical tests showed the method has acceptable accuracy, precision, and sensitivity. Clinical tests on FFPE lung samples demonstrated 93% diagnostic accuracy, similar to tests on fresh samples.
This document describes the development and validation of screening panels using flow cytometry to identify hematolymphoid malignancies. Specifically, it details the development of a 14 antibody, 10 color myeloid/lymphoid screening tube and its validation in identifying lymphoma, leukemia, myelodysplastic syndromes, and other hematologic malignancies using over 1000 patient samples with high accuracy compared to standard diagnostics. It also describes developing a scoring system using this screening tube to help identify myelodysplastic syndromes.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMerck Life Sciences
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Rapid Methodologies for Biosafety Testing of Biologic TherapeuticsMilliporeSigma
Learn about existing and emerging methods to accelerate biosafety testing of biologic therapies.
Speed to market for biologic therapeutics is ever more critical. However, the critical safety tests for these molecules, for example screening for adventitious agents such as viral contaminants, can be time consuming as well as challenging and laborious. Join us for this webinar as we explore how rapid methodologies are being used to not only accelerate this process, but also enhance quality by reducing testing complexity. Existing technologies as well as emerging trends will be discussed, along with the implications these may have on the regulatory landscape.
In this webinar you will learn:
● Which existing and emerging technologies are having now, and will have in the future, an impact on biosaftey testing.
● The benefits as well as risks of employing rapid methods for biosafety screening.
● How the regulatory agencies are reacting to rapid testing methods as alternatives to existing methods.
Japan Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 860-page report provides a comprehensive analysis of the Japanese molecular diagnostics market, including major trends, technologies, applications, and key players. It forecasts test volumes and reagent sales for infectious diseases, genetic diseases, cancer, and other applications. The report profiles leading suppliers and identifies over 60 business opportunities in molecular diagnostic instruments, tests, software, and auxiliary products. It also examines market entry barriers, alternative strategies, and concerns for companies operating in this space.
The document discusses using PCR arrays to profile gene expression and epigenetics. PCR arrays allow researchers to analyze expression of up to 84 genes related to a pathway or disease using real-time PCR. They include controls to check for genomic DNA contamination and assay performance. As an example, the document describes how a researcher could use a PCR array to compare gene expression between metastatic and non-metastatic breast tumor samples.
Corona is here to stay and it is predicted that over 70% of population will get the infection (fortunately not all will fall sick or very sick). (Recovery rate of over 74% & Death rate around 2%).
A lot of confusion exists regarding testing for covid and what test to do, when and how to interpret these tests.
Compiled by Dr. Narendra Malhotra
Yes, the results are statistically significant since the confidence interval does not cross 1. The results are also precise since the confidence interval is narrow. There is no information given to determine if there is publication bias.
Innovative Molecular Diagnostic Technologies and Emerging MarketsReportsnReports
This document provides a 1050-page report on innovative molecular diagnostic technologies and emerging markets. It explores business and technology trends in major countries and provides forecasts for market size, test volumes, and shares of leading competitors over the next 5-10 years. The report also profiles leading market players, identifies opportunities in product development, and discusses strategies for companies to successfully capitalize on the growing molecular diagnostics market.
The document summarizes recent updates in tuberculosis (TB) diagnostics and treatment. It discusses improvements and gaps in diagnosing TB infection versus active disease. New World Health Organization recommendations for TB diagnostics are outlined. Challenges in aligning current diagnostics with new multidrug-resistant TB treatment options are described. The future of TB diagnostics includes biomarkers and moving away from reliance on sputum samples for diagnosis. New tests are needed to address diagnostic gaps and better monitor treatment response.
Precision medicine for oncology requires accurate and sensitive molecular characterization. However, sample degradation, polymerase errors, and sequencing errors reduce accuracy for sequencing genetic variants. By incorporating molecular tagged adapters in target enrichment, and using DNA probes that deliver extremely even and deep coverage, we are able to demonstrate a 300-fold reduction in false positives at or above 0.25% variant frequency. In this presentation, Dr Mirna Jarosz discusses these methods and how they can significantly reduce error rates in your sequencing data.
The document discusses standard laboratory practices and standard operating procedures (SOPs) developed by the Health Protection Agency (HPA) in the UK. It provides an overview of the HPA's Standards and Evaluations units, which develop SOPs for clinical microbiology laboratories. It also discusses feedback from a survey of laboratories on the SOP for investigating genital tract specimens, including which aspects they found useful and suggestions for improvement. The talk concludes with plans to develop a new SOP for screening group B streptococcus from genital swabs.
US Molecular Diagnostics Market: Innovative Technologies and Emerging Busines...ReportsnReports
This 880-page report provides an extensive analysis of the US molecular diagnostics market, including forecasts of test volumes and reagent sales for various applications through 2022. It reviews DNA sequencing and probe technologies, detection methods, automated instrumentation, and profiles major suppliers. The report also identifies over 60 business opportunities in instruments, tests, software, and auxiliary products and discusses strategies for market entry.
1) Tuberculosis (TB) is commonly diagnosed through direct microscopy, culture, immunodiagnostic tests, molecular tests, and histopathology using samples from sputum, BAL, CSF, tissues, and other body fluids.
2) Direct microscopy has low sensitivity but is quick, while culture has higher sensitivity and allows drug susceptibility testing but takes 1-2 weeks for results. Newer liquid culture systems can provide results in only a few days.
3) Molecular tests like PCR and interferon-gamma release assays provide rapid results within hours and are also used for diagnosis, but many have high costs.
Italy Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 850-page report provides a comprehensive analysis of the Italian molecular diagnostics market, including major issues, trends, technologies, test categories, suppliers, and business opportunities. It forecasts test volumes and reagent sales for infectious diseases, genetic diseases, cancer, forensic testing, and other areas. The report profiles leading suppliers and identifies over 60 market opportunities. It also discusses alternative market strategies, barriers, and competitive landscapes. An executive summary analyzes the Italian business environment and market structure.
A simple and rapid dna extraction method from FINA nd qPCRManish Thakur
A simple and rapid DNA extraction method from whole blood for highly sensitive detection and quantitation of HIV-1 pro-viral DNA by real-time PCR (Journal of Virological Methods 214 (2015) 37–42 )
Spain Molecular Diagnostics Market: Innovative Technologies and Emerging Busi...ReportsnReports
This 860-page report provides a comprehensive analysis of the molecular diagnostics market in Spain, including key trends, technologies, major applications and suppliers. It covers topics such as DNA sequencing, probe technologies, detection methods, instrumentation, emerging applications in oncology, infectious diseases and pharmacogenomics. The report also identifies over 60 specific business opportunities and strategies for companies to enter the Spanish molecular diagnostics market.
Promoting Wellbeing - Applied Social Psychology - Psychology SuperNotesPsychoTech Services
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
One health condition that is becoming more common day by day is diabetes.
According to research conducted by the National Family Health Survey of India, diabetic cases show a projection which might increase to 10.4% by 2030.
Our backs are like superheroes, holding us up and helping us move around. But sometimes, even superheroes can get hurt. That’s where slip discs come in.
Osteoporosis - Definition , Evaluation and Management .pdfJim Jacob Roy
Osteoporosis is an increasing cause of morbidity among the elderly.
In this document , a brief outline of osteoporosis is given , including the risk factors of osteoporosis fractures , the indications for testing bone mineral density and the management of osteoporosis
We specifically measure ZAP-70 in CLL cells defined by CD19 and CD5 coexpression. We also measure ZAP-70 in T cells defined by CD3 and CD5 coexpression. Including FS and SS, each cell is defined by 5 parameters.