1. The document presents information on liposomal formulations and their preparation using a factorial design approach. Liposomes are spherical vesicles made of lipids that can be used to deliver drugs. Factorial design allows investigation of how formulation factors independently and interactively affect dependent variables like encapsulation efficiency and drug deposition.
2. A 32 factorial design was used to prepare liposomes from phospholipids and cholesterol, encapsulating Vitamin E. The design studied the effects of phospholipid amount and cholesterol amount on vesicle size, encapsulation efficiency, and drug deposition in rat skin.
3. The best performing liposomes based on drug deposition were selected for further formulation into gels using Carbopol polymer. The liposomal gel showed potential
A liposome is a closed, spherical lipid bilayer, which forms an internal cavity capable of carrying aqueous solutions. A lipid bilayer is composed of two sheets of tightly arranged phospholipids. These molecules have a hydrophobic tail and a hydrophilic head region. When two single membranes come together, the hydrophobic tails attract toward each other, while the heads of both membranes are attracted to the surrounding water. This forms a double layer of phospholipid molecules, which exclude the internal solution from the outside. The solution can then be transported with the liposome where it is needed.
A liposome should not be confused with either a micelle or a lysosome. A micelle is similar to a liposome in that it is a sphere of phospholipids. However a micelle is composed of a single layer and therefore does not have an aqueous interior. A lysosome is a specialized organelle in cells which separates caustic enzymes from the interior of the cell. While it too is similar to a liposome, it has many specialized proteins embedded in its membrane which help it function as an organelle within the cell.
A liposome is a closed, spherical lipid bilayer, which forms an internal cavity capable of carrying aqueous solutions. A lipid bilayer is composed of two sheets of tightly arranged phospholipids. These molecules have a hydrophobic tail and a hydrophilic head region. When two single membranes come together, the hydrophobic tails attract toward each other, while the heads of both membranes are attracted to the surrounding water. This forms a double layer of phospholipid molecules, which exclude the internal solution from the outside. The solution can then be transported with the liposome where it is needed.
A liposome should not be confused with either a micelle or a lysosome. A micelle is similar to a liposome in that it is a sphere of phospholipids. However a micelle is composed of a single layer and therefore does not have an aqueous interior. A lysosome is a specialized organelle in cells which separates caustic enzymes from the interior of the cell. While it too is similar to a liposome, it has many specialized proteins embedded in its membrane which help it function as an organelle within the cell.
Liposome based drug delivery system-boc sciencesBOC Sciences
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Liposomes are simple microscopic vesicles in which an aqueous volume is entirely enclosed by a membrane composed of lipid molecules. As drug carriers, liposomes can be loaded with a great variety of molecules, such as small drug molecules, proteins, nucleotides, and even plasmids. Please visit https://liposomes.bocsci.com for more information.
In this presentation, we will delve into the fascinating world of liposomes, microscopic spheres with a unique structure that holds immense potential in various fields. We will explore their composition, properties, and diverse applications, shedding light on their remarkable capabilities. Liposomes, with their biocompatible and versatile nature, offer a promising approach for drug delivery. We will delve deeper into their characteristics and explore how they can be harnessed for therapeutic advancements. Liposomes are fascinating structures resembling microscopic spheres. Their defining characteristic is their phospholipid bilayer membrane, which mimics the structure of cell membranes. This bilayer consists of phospholipid molecules, each with a hydrophilic head and a hydrophobic tail. The hydrophilic heads face the outer aqueous environment, while the hydrophobic tails orient themselves inwards. This unique arrangement creates an aqueous core within the liposome, providing a valuable space for encapsulating drugs or other molecules. One of the key advantages of liposomes is their biocompatibility. Their similarity to cell membranes allows them to interact with the body's systems without causing significant adverse effects. Additionally, liposomes offer stability, encapsulating drugs and protecting them from degradation in the body's environment. Moreover, liposomes are remarkably versatile. By modifying their surface properties, we can target them to deliver drugs to specific cells or tissues, enhancing the efficacy of treatment. Finally, liposomes provide the potential for controlled drug release. The rate at which encapsulated drugs are released can be regulated, allowing for sustained or targeted delivery.
The name liposome is derived from two Greek words: Lipo meaning âfatâ and Soma meaning âbodyâ.
Liposome are also defined as artificial microscopic vesicles consisting of aqueous compartment and surrounded by one or more concentric layer of phospholipid.
The sphere like interior encapsulates a liquid and also contain more substance like peptides, protein, hormones, enzymes, antibiotic, antifungal and anticancer agents.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
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This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Liposome based drug delivery system-boc sciencesBOC Sciences
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Liposomes are simple microscopic vesicles in which an aqueous volume is entirely enclosed by a membrane composed of lipid molecules. As drug carriers, liposomes can be loaded with a great variety of molecules, such as small drug molecules, proteins, nucleotides, and even plasmids. Please visit https://liposomes.bocsci.com for more information.
In this presentation, we will delve into the fascinating world of liposomes, microscopic spheres with a unique structure that holds immense potential in various fields. We will explore their composition, properties, and diverse applications, shedding light on their remarkable capabilities. Liposomes, with their biocompatible and versatile nature, offer a promising approach for drug delivery. We will delve deeper into their characteristics and explore how they can be harnessed for therapeutic advancements. Liposomes are fascinating structures resembling microscopic spheres. Their defining characteristic is their phospholipid bilayer membrane, which mimics the structure of cell membranes. This bilayer consists of phospholipid molecules, each with a hydrophilic head and a hydrophobic tail. The hydrophilic heads face the outer aqueous environment, while the hydrophobic tails orient themselves inwards. This unique arrangement creates an aqueous core within the liposome, providing a valuable space for encapsulating drugs or other molecules. One of the key advantages of liposomes is their biocompatibility. Their similarity to cell membranes allows them to interact with the body's systems without causing significant adverse effects. Additionally, liposomes offer stability, encapsulating drugs and protecting them from degradation in the body's environment. Moreover, liposomes are remarkably versatile. By modifying their surface properties, we can target them to deliver drugs to specific cells or tissues, enhancing the efficacy of treatment. Finally, liposomes provide the potential for controlled drug release. The rate at which encapsulated drugs are released can be regulated, allowing for sustained or targeted delivery.
The name liposome is derived from two Greek words: Lipo meaning âfatâ and Soma meaning âbodyâ.
Liposome are also defined as artificial microscopic vesicles consisting of aqueous compartment and surrounded by one or more concentric layer of phospholipid.
The sphere like interior encapsulates a liquid and also contain more substance like peptides, protein, hormones, enzymes, antibiotic, antifungal and anticancer agents.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
Â
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Embracing GenAI - A Strategic ImperativePeter Windle
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Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Synthetic Fiber Construction in lab .pptxPavel ( NSTU)
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Synthetic fiber production is a fascinating and complex field that blends chemistry, engineering, and environmental science. By understanding these aspects, students can gain a comprehensive view of synthetic fiber production, its impact on society and the environment, and the potential for future innovations. Synthetic fibers play a crucial role in modern society, impacting various aspects of daily life, industry, and the environment. ynthetic fibers are integral to modern life, offering a range of benefits from cost-effectiveness and versatility to innovative applications and performance characteristics. While they pose environmental challenges, ongoing research and development aim to create more sustainable and eco-friendly alternatives. Understanding the importance of synthetic fibers helps in appreciating their role in the economy, industry, and daily life, while also emphasizing the need for sustainable practices and innovation.
A Strategic Approach: GenAI in EducationPeter Windle
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Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
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In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Operation âBlue Starâ is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
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Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
⢠The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
⢠The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate âany matterâ at âany timeâ under House Rule X.
⢠The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Liposomal formulation by factorial design approach By Pranav Lendhey
1. Presented by,
Mr. Pranav S. Lendhey
M. Pharm I Year, roll no 06
Department of Pharmaceutics
Bharati Vidyapeeth College Of pharmacy, kolhapur
Liposomal formulation by factorial design approach
2. What is Liposome?
⢠The word liposome derives from two Greek words: lipo ("fat")
and soma ("body"); it is so named because its composition is primarily of
phospholipid.A liposome is a small artificial vesicle, spherical in shape, having
at least one lipid bilayer.
⢠Due to their hydrophobicity and/or hydrophilicity , biocompatibility, particle size
and many other properties
⢠liposomes can be used as drug delivery vehicles for administration
of pharmaceutical drugs and nutrients , such as lipid nanoparticles in mRNA
vaccines, and DNA vaccines.
⢠Liposomes can be prepared by disrupting biological membranes (such as
by sonication.)
3. The major types of liposomes are
⢠the multilamellar vesicle (MLV, with several lamellar phase lipid
bilayers,
⢠the small unilamellar liposome vesicle (SUV, with one lipid bilayer),
⢠the large unilamellar vesicle (LUV), and the cochleate vesicle. A less
desirable form are multivesicular liposomes in which one vesicle
contains one or more smaller vesicles.
4. * Factorial design is a statistical experimental design used to investigate the
effects of two or more independent variables (factors) on a dependent variable By
manipulating the levels of the characteristics and measuring the resulting impact
on the dependent variable,
* Researchers can identify each elementâs unique contributions and their
combined or interactive effects.
* Factorial designs allow researchers to look at how multiple factors affect a
dependent variable, both independently and together.
* Factorial design studies are named for the number of levels of the factors. A
study with two factors that each have two levels, for example, is called a 2x2
factorial design
What is factorial design ?
5. ⢠These are beneficial when investigating interactions between variables. They
allow researchers to explore how one factorâs effects may depend on another
elementâs levels. This can provide valuable insights into the underlying
mechanisms. It further drives the observed impacts and helps identify potential
moderators or mediators of the relationship between variables.
7. In last two decades, number of innovative microparticulate carrier systems viz.
⢠microemulsion
⢠nanoemulsion
⢠nanoparticles
⢠liposomes
⢠ethosomes, etc. have been reported for improving delivery of drug to the skin.
⢠Recently, liposome based formulations for topical delivery have been shown to be
extremely promising for
1.enhancement of drug penetration
2. improved pharmacological effect
3.decreased side effects
4.controlled drug release
5.drug photoprotection.
reported encapsulation of Vitamin A palmitate into liposomes protects it from
photo degradation
8. ⢠It is demonstrated that delivery of sodium ascorbyl palmitate incorporated in
liposomal formulation can enhance the drug deposition and helps to prevent or
slowing down the complex process of photoaging in the skin.
⢠Improved delivery of drugs using liposomes is based on similarity of vesicle
bilayer structure to that of natural membranes, which can alter cell membrane
fluidity and fuse with cells
⢠The phenomenon of improved drug delivery is based on factors viz. lipid
concentration, composition, lamellarity, vesicle size, surface charge, type of
formulation
⢠Encapsulation efficiency (EE), drug deposition and vesicle size were the key
parameters involved in formulation of liposome
⢠The number of formulation and processing variables are involved during liposome
preparation may affect these parameters and hence the performance of the
formulation Thus it becomes extremely difficult to study the effect of interactions
between various variables and preparation of liposome by a conventional method
9. ⢠The number of formulation and processing variables are involved during liposome
preparation may affect these parameters and hence the performance of the
formulation Thus it becomes extremely difficult to study the effect of interactions
between various variables and preparation of liposome by a conventional method.
⢠Thus the aim of the present investigation was to carry out a systematic statistical
study on preparation of liposomal formulation using factorial design approach and
to explore its application for topical delivery of Vitamin E acetate (VE). Relative
quantities of phospholipid and cholesterol (PL:CH) is an important parameter in
light of the stability and cost consideration. Their ratio also greatly affects vesicle
size and drug deposition in the skin. In the present study, liposomes were prepared
by 32 factorial design using a modified ethanol injection method.
⢠However, VE was selected by predicting 100% encapsulation owing to its
lipophillic nature, which will more effectively help in studying the effect of the
formulation variables. Liposomes were characterized by Encapsulation efficiency
[EE], vesicle size, zeta potential, and drug deposition in the rat skin. Gels
containing liposomal dispersion (batch with higher skin deposition of VE) were
prepared using CarbopolÂŽ 980 NF and were characterized for gel strength,
viscosity and drug deposition in rat skin. Stability of liposome dispersion and gel
formulation was studied at 30 âŚC/65% RH for 3 months.
10. Preparation of liposomes
Liposomes were prepared by modified ethanol injection method as described by
Batzri and Korn (1973). In brief, required amount of P 80N, CH and SA (Table 1)
were dissolved in 1 ml of ethanol containing VE (12 mg). Ethanolic solution was
rapidly injected into 10 ml of double distilled water (DDW) under stirring at 500
rpm for 15 min using Teflon coated magnetic head
Effect of variables:-
To study the effect of variables on liposome performance and characteristics,
different batches were prepared using 32 factorial design. Amount of P 80N and CH
were selected as two independent variables. Vesicle size, EE and drug deposition
inthe rat skin were selected as dependent variables. Amount of SA and VE were kept
constant
11. Preparation of liposome gels On the basis of
factorial design approach
⢠liposomal batch (LP-6) was selected for further formulation studies of liposomal
gel. Gels were prepared using CarbopolÂŽ 980 NF at different concentrations.
⢠Briefly, required amount of CarbopolŽ 980 NF was added into water and kept
overnight for complete humectation of polymer chains.
⢠Liposome dispersion (LP-6) was added to hydrated carbopol solution with stirring
to give final concentration of 0.1, 0.3 and 0.5% (w/w).
⢠Gelling was induced by neutralizing the dispersions to pH 6.8â7.0 using 18%
(w/v) NaOH solution.
12. Conclusion :-
⢠Preparation of liposomes using factorial design was found to be well suited and
sound approach to obtain stable liposomal formulation.
⢠Loading of the highly lipophillic drug such as Vitamin E in the liposomes greatly
influences the micromeritic properties of the vesicle.
⢠Variables such as amount of phospholipid, amount of stabilizer and lipid:drug ratio
have a profound effect on the vesicle size and drug deposition in the rat skin.
Increased drug deposition in rat skin as compared to control drug dispersion,
control gel and marketed cream suggest that liposomal formulation promotes drug
deposition in the at skin thus has potential for dermal delivery. Liposomal
dispersion and liposomal gel were found to be stable for 3 months at 30 âŚC and
65% RH.