Nice

1. DEFENSE MECHANISM OF THE
GINGIVA
 Mechanical & bacterial
aggressions
 Saliva , epithelial surface , initial
stages of inflammatory response

2. SULCULAR FLUID
 (GCF) 19 century
 Investigatores (use filter paper)
 By the name of fluoroscent
 Fluid (from bloodstream through the
tissue exiting via gingival sulcus)
 Brill (Transudate)

3. METHOD OF COLLECTION
1. Use of absorbing paper stirps
2. Twisted threads placed around and
into the sulcus
3. Micropipettes
4. Intracrevicular washing

4. PERMEABILITY OF JE
AND SE
The permeable materials :
 Albumin
 Endotoxin
 Thymidine
 Histamine
 Phenytion
 Horseradish peroxidase

5. CONT…
This amount is extremely small
CIMASONI:
Strip of paper 1.5mm wide
&inserted 1 mm within gingival
sulcus (in 3 minutes)

6. COMPOSITION
 Proteins
 Specific Antibodies
 Antigens
 Enzymes
 Also contain Cellular elements

7. CONT…
 More than 40 component found in GCF
 Beta – glucuronidase ( Lysosome enzyme)
 Lactic Acid dehydrogenase (Cytoplasmic
enzyme)
 SOURCE OF COLLAGENASE :
 Fibroblast , PMN leukocyte
 PHASPHOLIPASE :
 Lysosomal , cytoplasmic , also
(microorganism)

8. CELLULAR ELEMENTS :
 INCLUDE:
 bacteria
 Desquamated epithelial cell
 Leukocyte (PMN , lymphocyte ,
monocyte/macrophage)
 migrate through sulcular epithelium

9. CONT…
 ROLE OF ANTIBODIES :
1. Reduction in antibody response is
detrimental
2. Antibody response plays protective
role

10. CLINICAL SIGNIFICANCE
 GCF is inflammatory exudates
 Greater in inflammation
 Also increase due to :
 Occlusal trauma , mastication of hard
food , tooth brushing and gingival
massage

11. DRUGS IN GCF
 are extracted through the GCF
 Bader and Goldhaber :
 Tetracycline & Metronidazole

12. SALIVA