1. Tissue Engineering of Nasal Septum Using Chitosan-
Based Material
by
Arunkumar rengaraj
(22141800)

2. Nasal septum
Nasal septum divides the nasal chamber into two cavities.
The turbinates are made of bone and soft tissue.
Conventional repair of nasal septum involves autologous rib or septum cartilage grafts and prosthetic devices.
Its having limitations like tissue availability, and prosthesis related infection and extrusion.
Major reason leading to the failure
Lack of sufficient control over shape is the lack of appropriate scaffolds
Less biocompatibility for cell seeding

3. Chitosan
• Chitosan, a linear polysaccharide derived from crustacean shells and from many natural sources.
• It having advantages like polycationic nature, biodegradability, biocompatibility, mucoadhesiveness.
• In addition, it enhances epithelial permeability through the opening of tight junctions between epithelial cells.

4. Glycosaminoglycans
It is long unbranched polysaccharides.
GAGs in enhancing chondrogenesis in vitro.
One of the chief components of the extracellular matrix .
It contributes significantly to cell proliferation and migration.
Hyaluronan recognizing receptor
• CD44
• Receptor for HA-mediated motility (RHAMM)
• Intercellular adhesion molecule-1 (ICAM-1)
It may also be used postoperatively to induce tissue healing.

5. Materials and methods
2 % solution of chitosan + 1 M acetic acid
Add Hyaluronic acid/ MeOH 1:1
Mix 40 μg type I collagen
Hybrid polymer

6. Tissues (Human inferior turbinates ) were treated with 0.5% pronase+1:1 mixture (DMEM) +
Ham’s nutrient F12
centrifugation
suspended in DMEM/F12
1.5-mL cell suspension was seeded on collagen coated
Morphological Examination
• Calculate the pore size of the mess
• The DNA content of the cell have to study for calculating the proliferation.
• The ciliary beat was observed under inverted microscope (Leica, DMI 6000, Solms, Germany) with 400 magnification.
• Imaging of cilia movement was recorded at 240 frames per second with a high-speed complementary metal oxide
semiconductor (CMOS) camera (CMC1300; VDS Vossku¨ hler GmbH, Osnabru¨ ck, Germany).
• Histological and immuno-histochemical apperance of the tissue.
• Finally want to perform animal study.

7. Reference
• Dong-Won Lee, Shawna A Shirley, Richard F Lockey and Shyam Mohapatra. Thiolated chitosan nanoparticles enhance anti-inflammatory
effects of intranasally delivered theophylline. Respiratory Research 2006; 7:112.
• Tsung-Wei Huang, Yi-Ho Young, Po-Wen Cheng, Yen-Hui Chan, Tai-Horng Young. Culture of Nasal Epithelial Cells Using
Chitosan-Based Membranes. The Laryngoscope 2009; 119:2066-2070.