Lactic acid bacteria (LAB) such as Lactobacillus, Lactococcus, Leuconostoc, and Pediococcus are important in food fermentation processes. They produce lactic acid which preserves foods and improves safety. Lactobacillus is the largest LAB genus and includes species used in dairy, bread, meat and vegetable fermentations. Lactococcus lactis is used as a starter culture for cheeses and cultured dairy. These LAB vary in their temperature and pH preferences, as well as metabolic pathways, contributing to flavor development in fermented foods through production of organic acids, aromas, and proteolysis.
This document discusses lactic acid bacteria (LAB) and their potential use as vaccines. It outlines that LAB naturally colonize mucosal membranes and could serve as ideal mucosal vaccine delivery vehicles. Examples are given of LAB like Lactococcus lactis being genetically engineered to express antigens from pathogens like Brucella abortis and Helicobacter pylori. The benefits of LAB vaccines are their safety, ability to survive the stomach, and lack of endotoxicity. Future work aims to develop multi-valent LAB vaccine vectors in clinical trials with biological containment to ensure environmental safety.
This document discusses various lactic acid bacteria used in food fermentations. It describes the classification and characteristics of important genera used as starter cultures including Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. It also provides background on the taxonomy and phylogeny of these microorganisms based on 16S rRNA sequencing and their roles in important fermented foods like cheese, yogurt, sausages and vegetables.
A starter culture is a culture of bacteria used to control the fermentation of milk. It is desirable because the natural microflora in milk can be unpredictable and inconsistent. A starter culture provides a controlled and predictable fermentation. There are different types of starter cultures defined by factors like the bacteria used, temperature optimum, physical form, and whether they contain single or multiple bacterial species. Proper preparation and maintenance of the starter culture is important to ensure it performs as intended in fermenting milk.
Antimicrobial metabolites of lactic acid bacteria and its applicationDiwas Pradhan
This document discusses lactic acid bacteria and their antimicrobial metabolites. It begins with an overview of the taxonomy of lactic acid bacteria and describes some of the organic acids, bacteriocins, and other low molecular weight antimicrobial compounds they can produce. It then discusses applications of these compounds in food preservation and safety, as well as medical and veterinary uses. Specifically, it explores using purified/semi-purified bacteriocins as food additives and fermenting foods or ingredients with bacteriocin-producing cultures. Overall, the document provides information on the antimicrobial compounds produced by lactic acid bacteria and their potential applications.
Probiotics, prebiotics, and synbiotics were defined. Probiotics are live microorganisms that provide health benefits when consumed. Prebiotics are non-digestible fibers that promote the growth of beneficial bacteria. Synbiotics combine probiotics and prebiotics. The document discussed the history of probiotic research from Metchnikoff's observations of Bulgarian longevity to current probiotic foods and strains. Potential health benefits of probiotics include managing diarrhea, allergies, and cholesterol, as well as supporting the immune system. Factors like processing, storage and the host's health impact probiotic survival.
This document discusses kefir, a fermented dairy beverage produced by inoculating milk with kefir grains. Kefir grains contain various lactic acid bacteria and yeasts that ferment the milk sugars to produce kefir's characteristic sour taste, slightly alcoholic and yeasty flavor. Kefir provides probiotics and nutrients like protein, calcium and B vitamins. It is prepared by mixing pasteurized milk with kefir grains, incubating to ferment, then separating the grains to produce a drinkable consistency high in beneficial microbes and nutrients. Kefir grains can be preserved through drying or refrigeration and reused to make repeated batches of the probiotic fermented milk beverage.
Lactobacilli- Homo and Hetero lactic acid Fermentation and its nutritive value pugazhenthi6
The document discusses lactic acid fermentation by lactic acid bacteria (LAB), specifically the genera Lactobacillus. It describes how LAB convert sugars into lactic acid through either homolactic fermentation, which produces only lactic acid, or heterolactic fermentation, which produces lactic acid as well as ethanol and carbon dioxide. Applications of homolactic fermentation include dairy products and probiotics, while heterolactic bacteria are involved in other fermentation processes.
This document discusses lactic acid bacteria (LAB) and their potential use as vaccines. It outlines that LAB naturally colonize mucosal membranes and could serve as ideal mucosal vaccine delivery vehicles. Examples are given of LAB like Lactococcus lactis being genetically engineered to express antigens from pathogens like Brucella abortis and Helicobacter pylori. The benefits of LAB vaccines are their safety, ability to survive the stomach, and lack of endotoxicity. Future work aims to develop multi-valent LAB vaccine vectors in clinical trials with biological containment to ensure environmental safety.
This document discusses various lactic acid bacteria used in food fermentations. It describes the classification and characteristics of important genera used as starter cultures including Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. It also provides background on the taxonomy and phylogeny of these microorganisms based on 16S rRNA sequencing and their roles in important fermented foods like cheese, yogurt, sausages and vegetables.
A starter culture is a culture of bacteria used to control the fermentation of milk. It is desirable because the natural microflora in milk can be unpredictable and inconsistent. A starter culture provides a controlled and predictable fermentation. There are different types of starter cultures defined by factors like the bacteria used, temperature optimum, physical form, and whether they contain single or multiple bacterial species. Proper preparation and maintenance of the starter culture is important to ensure it performs as intended in fermenting milk.
Antimicrobial metabolites of lactic acid bacteria and its applicationDiwas Pradhan
This document discusses lactic acid bacteria and their antimicrobial metabolites. It begins with an overview of the taxonomy of lactic acid bacteria and describes some of the organic acids, bacteriocins, and other low molecular weight antimicrobial compounds they can produce. It then discusses applications of these compounds in food preservation and safety, as well as medical and veterinary uses. Specifically, it explores using purified/semi-purified bacteriocins as food additives and fermenting foods or ingredients with bacteriocin-producing cultures. Overall, the document provides information on the antimicrobial compounds produced by lactic acid bacteria and their potential applications.
Probiotics, prebiotics, and synbiotics were defined. Probiotics are live microorganisms that provide health benefits when consumed. Prebiotics are non-digestible fibers that promote the growth of beneficial bacteria. Synbiotics combine probiotics and prebiotics. The document discussed the history of probiotic research from Metchnikoff's observations of Bulgarian longevity to current probiotic foods and strains. Potential health benefits of probiotics include managing diarrhea, allergies, and cholesterol, as well as supporting the immune system. Factors like processing, storage and the host's health impact probiotic survival.
This document discusses kefir, a fermented dairy beverage produced by inoculating milk with kefir grains. Kefir grains contain various lactic acid bacteria and yeasts that ferment the milk sugars to produce kefir's characteristic sour taste, slightly alcoholic and yeasty flavor. Kefir provides probiotics and nutrients like protein, calcium and B vitamins. It is prepared by mixing pasteurized milk with kefir grains, incubating to ferment, then separating the grains to produce a drinkable consistency high in beneficial microbes and nutrients. Kefir grains can be preserved through drying or refrigeration and reused to make repeated batches of the probiotic fermented milk beverage.
Lactobacilli- Homo and Hetero lactic acid Fermentation and its nutritive value pugazhenthi6
The document discusses lactic acid fermentation by lactic acid bacteria (LAB), specifically the genera Lactobacillus. It describes how LAB convert sugars into lactic acid through either homolactic fermentation, which produces only lactic acid, or heterolactic fermentation, which produces lactic acid as well as ethanol and carbon dioxide. Applications of homolactic fermentation include dairy products and probiotics, while heterolactic bacteria are involved in other fermentation processes.
Yogurt is produced through the controlled fermentation of milk by two bacteria, Lactobacillus bulgaricus and Lactotococcus thermophilus. These bacteria convert the milk's sugar (lactose) into lactic acid, which causes the characteristic yogurt curd to form. Their interaction also produces compounds like formic acid and carbon dioxide that stimulate further bacterial growth. The lactic acid produced causes the milk proteins to coagulate, thickening the yogurt. Additional flavors can be added to increase consumer popularity.
This document discusses solid state fermentation and provides details about the process. It describes that solid state fermentation involves fermentation using solids in the absence of free water, though some moisture is needed. Microorganisms like fungi grow on the surface of solid substrates to produce things like enzymes, organic acids, and flavors. Agriculture wastes are commonly used as substrates. Fungi like Trichoderma and Aspergillus species are widely used to produce hydrolytic enzymes. Tray fermenters and rotating drum reactors are two common types of bioreactors used in solid state fermentation.
This document discusses several types of fermented Asian foods including soy sauce, miso, sufu, natto, and idli. It describes the key ingredients and fermentation processes for each food. Soy sauce is produced from fermented soybeans, wheat, and saltwater using molds and bacteria. Miso is made from fermented soybeans with rice or barley and varies in taste depending on ingredients and fermentation time. Sufu involves drying and air fermenting tofu cubes with molds. Natto is made by fermenting soybeans with Bacillus subtilis. Idli involves the bacterial fermentation of rice and black gram dhal batter.
Fermented foods like cheese, yogurt, and kefir are produced through microbial fermentation. Microorganisms like bacteria, yeasts, and molds interact with foods biochemically, physically, and biologically to produce the final fermented product. In cheese production, a starter culture is added to pasteurized milk, which is fermented to produce curd. The curd is then drained, cut, scalded, stretched, milled, salted, and ripened to produce cheese. Yogurt is made by inoculating milk with bacterial cultures of Lactobacillus bulgaricus and Streptococcus thermophilus, which ferment the milk sugars to produce lactic acid and cause the milk to thicken
The document discusses probiotics, their history, functions, and food sources. It begins by defining probiotics as live microorganisms that provide health benefits when consumed in adequate amounts. The concept of probiotics was first proposed in the early 20th century by Elie Metchnikoff, who suggested certain bacteria in fermented milk could promote intestinal and overall health. The document then outlines the characteristics, mechanisms of action, advantages, and functions of probiotic consumption before providing examples of probiotic foods and the probiotic strains they contain.
Fermentation of vegetables and meat productsAman Kumar
This document discusses lactic acid fermentation of vegetables. It begins by explaining that vegetables naturally contain microflora that can be controlled through conditions like acidity or lack of nutrients. Lactic acid fermentation stabilizes this microflora. Starters are now used to initiate controlled fermentations. Many vegetables can undergo lactic acid fermentation including cabbage, carrots, cucumbers and olives. The process enhances quality and nutrition of the vegetables while restricting unwanted bacteria. Popular fermented vegetables from different regions like sauerkraut, kimchi and olives are then described in more detail.
Sauerkraut is a fermented cabbage product with a sour taste made through the lactic acid fermentation of shredded cabbage by lactic acid bacteria such as Leuconostoc mesenteroides and Lactobacillus plantarum. The fermentation process typically takes 4-8 weeks to produce sauerkraut with an acidity level of around 1.7% and beneficial probiotic bacteria. Sauerkraut is high in fiber, vitamins, and minerals and consumption has various health benefits such as supporting digestive health and reducing inflammation.
This document is a report submitted by Mr. E. Mari Karthick, a student at Sri Paramakalyani College in Alwarkurichi, on the topic of oriental fermented foods. The report was submitted for a food microbiology course and overseen by Dr. C. Mariappan, an assistant professor of microbiology at Sri Paramakalyani College.
Fermentation
Bread Definition
History
Types of bread
Steps in yeast bread production
Protocols
Steps in bread making
Components of bread
Benefits of bread
References
This document discusses various fermented milk products including cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It provides details on the production processes and microorganisms involved in each product. Cheese is produced through fermentation of milk proteins and fats using bacteria and ripening. Yogurt is made by fermenting milk with Lactobacillus bulgaricus and Streptococcus thermophilus. Cultured buttermilk is the fluid remaining after sour cream or ripened cream is churned into butter. Acidophilus milk contains Lactobacillus acidophilus for potential health benefits. Kefir uses "kefir grains" containing various bacteria and yeasts to ferment milk
The document discusses inoculum development and production media for industrial fermentation. It defines inoculum as a culture of microbes used to inoculate production-scale fermentations. Successful fermentations require developing inoculum to an active, healthy state in appropriate density. The document outlines factors that affect fermentation and discusses various media components like carbon sources, nitrogen sources, and trace elements. It also covers inoculum development methods for bacterial and mycelial cultures, preservation techniques, examples of media used for specific inocula, and criteria for a good inoculum.
Cheese production involves several key steps:
1) Curdling of milk through the addition of starter cultures or rennet, which causes casein to coagulate and separate from whey.
2) Draining the curd to remove moisture and separate whey.
3) Salting the curd, which acts as a preservative and controls moisture.
4) Ripening the curd through bacterial or mold cultures, during which flavor and texture develop.
Different cheeses are produced by varying the cultures, temperatures, and other conditions during the production process.
The document discusses several types of fermented vegetables including sauerkraut, kimchi, olives, and pickles. It describes the key ingredients and processes used to ferment each vegetable. Sauerkraut is made from fermented cabbage using a process of shredding, salting, and fermenting the cabbage for several weeks. Kimchi also uses fermented cabbage but includes additional spices and a shorter fermentation time. Olives undergo a lye treatment before being fermented in brine for 6-8 months. Pickles are made by fermenting cucumbers in a brine solution for several weeks. The dominant microorganisms in each fermentation process are various species of lactic
This document discusses fermented foods. It begins by explaining that fermented foods make up one third of the global human diet and include products like cheese, bread, fermented vegetables, and meats. It then defines fermented foods as foods produced or modified by microorganisms like bacteria, yeasts, and molds. The document goes on to describe various fermented foods and beverages from around the world, the microorganisms involved in fermentation, and the nutritional and health benefits of consuming fermented foods.
Did you find the soy sauce kept at your home spoiled? You made sure that it is kept in the desired storage conditions but still found the soy sauce without it's characteristic flavor. Have a look at this presentation to know the reason behind the spoilage of Soy Sauce & the various microbiological aspects responsible for the spoilage.
This document discusses the microbiology of idly, a popular South Indian breakfast food. It begins by introducing idly and its variations. The key microorganisms involved in idly fermentation are lactic acid bacteria like Leuconostoc mesenteroidies and yeasts like Saccharomyces cerevisiae. These microbes lower the pH and increase volume during fermentation. Biochemical changes increase nutrients like proteins, amino acids, and vitamins. Finally, the document discusses methods to improve the shelf life of idly batter and cooked idly.
Sauerkraut is finely cut cabbage that is fermented by various lactic acid bacteria, including Leuconostoc mesenteroides, Lactobacillus plantarum, and Lactobacillus pentoaceticus. The fermentation process sour's the cabbage and gives it a long shelf life. Sauerkraut is recommended for treatment of overweight, metabolic disorders, and detoxification. To make sauerkraut, shredded cabbage is packed in a container with salt added which draws out juice for microbial fermentation. Proper temperature, salt levels, and starter cultures are needed to ensure consistent high quality fermentation.
Lactobacillus is a genus of bacteria that is a major part of the lactic acid bacteria group. They convert sugars into lactic acid. Lactobacillus inhabit mammalian mucosa and areas with rich carbohydrates. They are used as starter cultures in food production due to their ability to suppress pathogens. Lactobacillus have various applications in the pharmaceutical, food, and dairy industries including production of bacteriocins, lactic acid, and providing beneficial effects to the human microbiome and immunity.
This document provides an overview of the genus Lactobacillus. It discusses the history of isolation of various Lactobacillus species from different sources. It covers the taxonomy, morphology, habitat, metabolism, roles in food production and as probiotics, pathogenesis, and cultural and biochemical characteristics of Lactobacillus. The document also includes images showing growth of Lactobacillus in different media and biochemical test results.
Yogurt is produced through the controlled fermentation of milk by two bacteria, Lactobacillus bulgaricus and Lactotococcus thermophilus. These bacteria convert the milk's sugar (lactose) into lactic acid, which causes the characteristic yogurt curd to form. Their interaction also produces compounds like formic acid and carbon dioxide that stimulate further bacterial growth. The lactic acid produced causes the milk proteins to coagulate, thickening the yogurt. Additional flavors can be added to increase consumer popularity.
This document discusses solid state fermentation and provides details about the process. It describes that solid state fermentation involves fermentation using solids in the absence of free water, though some moisture is needed. Microorganisms like fungi grow on the surface of solid substrates to produce things like enzymes, organic acids, and flavors. Agriculture wastes are commonly used as substrates. Fungi like Trichoderma and Aspergillus species are widely used to produce hydrolytic enzymes. Tray fermenters and rotating drum reactors are two common types of bioreactors used in solid state fermentation.
This document discusses several types of fermented Asian foods including soy sauce, miso, sufu, natto, and idli. It describes the key ingredients and fermentation processes for each food. Soy sauce is produced from fermented soybeans, wheat, and saltwater using molds and bacteria. Miso is made from fermented soybeans with rice or barley and varies in taste depending on ingredients and fermentation time. Sufu involves drying and air fermenting tofu cubes with molds. Natto is made by fermenting soybeans with Bacillus subtilis. Idli involves the bacterial fermentation of rice and black gram dhal batter.
Fermented foods like cheese, yogurt, and kefir are produced through microbial fermentation. Microorganisms like bacteria, yeasts, and molds interact with foods biochemically, physically, and biologically to produce the final fermented product. In cheese production, a starter culture is added to pasteurized milk, which is fermented to produce curd. The curd is then drained, cut, scalded, stretched, milled, salted, and ripened to produce cheese. Yogurt is made by inoculating milk with bacterial cultures of Lactobacillus bulgaricus and Streptococcus thermophilus, which ferment the milk sugars to produce lactic acid and cause the milk to thicken
The document discusses probiotics, their history, functions, and food sources. It begins by defining probiotics as live microorganisms that provide health benefits when consumed in adequate amounts. The concept of probiotics was first proposed in the early 20th century by Elie Metchnikoff, who suggested certain bacteria in fermented milk could promote intestinal and overall health. The document then outlines the characteristics, mechanisms of action, advantages, and functions of probiotic consumption before providing examples of probiotic foods and the probiotic strains they contain.
Fermentation of vegetables and meat productsAman Kumar
This document discusses lactic acid fermentation of vegetables. It begins by explaining that vegetables naturally contain microflora that can be controlled through conditions like acidity or lack of nutrients. Lactic acid fermentation stabilizes this microflora. Starters are now used to initiate controlled fermentations. Many vegetables can undergo lactic acid fermentation including cabbage, carrots, cucumbers and olives. The process enhances quality and nutrition of the vegetables while restricting unwanted bacteria. Popular fermented vegetables from different regions like sauerkraut, kimchi and olives are then described in more detail.
Sauerkraut is a fermented cabbage product with a sour taste made through the lactic acid fermentation of shredded cabbage by lactic acid bacteria such as Leuconostoc mesenteroides and Lactobacillus plantarum. The fermentation process typically takes 4-8 weeks to produce sauerkraut with an acidity level of around 1.7% and beneficial probiotic bacteria. Sauerkraut is high in fiber, vitamins, and minerals and consumption has various health benefits such as supporting digestive health and reducing inflammation.
This document is a report submitted by Mr. E. Mari Karthick, a student at Sri Paramakalyani College in Alwarkurichi, on the topic of oriental fermented foods. The report was submitted for a food microbiology course and overseen by Dr. C. Mariappan, an assistant professor of microbiology at Sri Paramakalyani College.
Fermentation
Bread Definition
History
Types of bread
Steps in yeast bread production
Protocols
Steps in bread making
Components of bread
Benefits of bread
References
This document discusses various fermented milk products including cheese, yogurt, cultured buttermilk, acidophilus milk, and kefir. It provides details on the production processes and microorganisms involved in each product. Cheese is produced through fermentation of milk proteins and fats using bacteria and ripening. Yogurt is made by fermenting milk with Lactobacillus bulgaricus and Streptococcus thermophilus. Cultured buttermilk is the fluid remaining after sour cream or ripened cream is churned into butter. Acidophilus milk contains Lactobacillus acidophilus for potential health benefits. Kefir uses "kefir grains" containing various bacteria and yeasts to ferment milk
The document discusses inoculum development and production media for industrial fermentation. It defines inoculum as a culture of microbes used to inoculate production-scale fermentations. Successful fermentations require developing inoculum to an active, healthy state in appropriate density. The document outlines factors that affect fermentation and discusses various media components like carbon sources, nitrogen sources, and trace elements. It also covers inoculum development methods for bacterial and mycelial cultures, preservation techniques, examples of media used for specific inocula, and criteria for a good inoculum.
Cheese production involves several key steps:
1) Curdling of milk through the addition of starter cultures or rennet, which causes casein to coagulate and separate from whey.
2) Draining the curd to remove moisture and separate whey.
3) Salting the curd, which acts as a preservative and controls moisture.
4) Ripening the curd through bacterial or mold cultures, during which flavor and texture develop.
Different cheeses are produced by varying the cultures, temperatures, and other conditions during the production process.
The document discusses several types of fermented vegetables including sauerkraut, kimchi, olives, and pickles. It describes the key ingredients and processes used to ferment each vegetable. Sauerkraut is made from fermented cabbage using a process of shredding, salting, and fermenting the cabbage for several weeks. Kimchi also uses fermented cabbage but includes additional spices and a shorter fermentation time. Olives undergo a lye treatment before being fermented in brine for 6-8 months. Pickles are made by fermenting cucumbers in a brine solution for several weeks. The dominant microorganisms in each fermentation process are various species of lactic
This document discusses fermented foods. It begins by explaining that fermented foods make up one third of the global human diet and include products like cheese, bread, fermented vegetables, and meats. It then defines fermented foods as foods produced or modified by microorganisms like bacteria, yeasts, and molds. The document goes on to describe various fermented foods and beverages from around the world, the microorganisms involved in fermentation, and the nutritional and health benefits of consuming fermented foods.
Did you find the soy sauce kept at your home spoiled? You made sure that it is kept in the desired storage conditions but still found the soy sauce without it's characteristic flavor. Have a look at this presentation to know the reason behind the spoilage of Soy Sauce & the various microbiological aspects responsible for the spoilage.
This document discusses the microbiology of idly, a popular South Indian breakfast food. It begins by introducing idly and its variations. The key microorganisms involved in idly fermentation are lactic acid bacteria like Leuconostoc mesenteroidies and yeasts like Saccharomyces cerevisiae. These microbes lower the pH and increase volume during fermentation. Biochemical changes increase nutrients like proteins, amino acids, and vitamins. Finally, the document discusses methods to improve the shelf life of idly batter and cooked idly.
Sauerkraut is finely cut cabbage that is fermented by various lactic acid bacteria, including Leuconostoc mesenteroides, Lactobacillus plantarum, and Lactobacillus pentoaceticus. The fermentation process sour's the cabbage and gives it a long shelf life. Sauerkraut is recommended for treatment of overweight, metabolic disorders, and detoxification. To make sauerkraut, shredded cabbage is packed in a container with salt added which draws out juice for microbial fermentation. Proper temperature, salt levels, and starter cultures are needed to ensure consistent high quality fermentation.
Lactobacillus is a genus of bacteria that is a major part of the lactic acid bacteria group. They convert sugars into lactic acid. Lactobacillus inhabit mammalian mucosa and areas with rich carbohydrates. They are used as starter cultures in food production due to their ability to suppress pathogens. Lactobacillus have various applications in the pharmaceutical, food, and dairy industries including production of bacteriocins, lactic acid, and providing beneficial effects to the human microbiome and immunity.
This document provides an overview of the genus Lactobacillus. It discusses the history of isolation of various Lactobacillus species from different sources. It covers the taxonomy, morphology, habitat, metabolism, roles in food production and as probiotics, pathogenesis, and cultural and biochemical characteristics of Lactobacillus. The document also includes images showing growth of Lactobacillus in different media and biochemical test results.
This document provides an overview of the metabolism and genetics of lactic acid bacteria (LAB) used as starter cultures in food fermentation. LAB play an important role in fermented foods through the production of lactic acid and other beneficial compounds. The three main metabolic pathways involved are glycolysis (sugar fermentation), lipolysis (fat degradation), and proteolysis (protein degradation). Advances in genetics and genomics have revealed insights into LAB metabolism and led to commercial starter cultures with desirable properties for fermented foods.
The document discusses the Lactobacillales order of bacteria. It notes that they are Gram-positive, non-sporeforming rods or cocci that produce lactic acid as a major fermentation product. They can be facultatively anaerobic and are found in environments like plants, milk, and the mouths and guts of humans and animals. The document separates Lactobacillales into families based on morphology and metabolism, and provides examples of medically important and industrially used species.
This study aimed to isolate and characterize lactic acid bacteria from dairy products in Egypt with probiotic potential. Fifty-four isolates were obtained from samples including human milk, yogurt and raw milk. Eight isolates from different dairy products were found to be tolerant to low pH and bile salt and had antagonistic effects against pathogenic bacteria. Biochemical and physiological testing identified the isolates as belonging to Lactobacillus casei, L. acidophilus, and L. lactis. The isolates were also found to produce enzymes and have no hemolytic activity, indicating their potential as safe and effective probiotics.
This document discusses microorganisms used in food fermentation. It describes the criteria for selecting microorganisms, including that they must be safe, food-grade, and approved. It also discusses the microbiology of fermented foods, including that fermentation involves microbes converting raw materials and producing end products. Common genera used in fermentation are also listed, including Lactococcus, Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. Species from these genera are frequently used as starter cultures.
α-Galactosidase Producing Probiotics Bacteria and Their Health ImplicationsSUS GROUP OF INSTITUTIONS
Nowadays, people are aware that diet plays a major role in preventing diseases and promoting health.
Therefore there is an increasing trend for functional foods containing probiotic culture. “Probiotics are
defined as live microorganisms which when administered in adequate amounts confer a health benefit
on the host”. Some LAB positively influence human health mainly by improving the composition of
intestinal micro biota and for this reason, they are called probiotics. The increasing cost of health care,
the steady increase in life expectancy and the desire of the elderly for improved quality of life research
and development required in the area of probiotics. The concept of providing functional foods
including beneficial components rather than removing potentially harmful components. Soybeans
and other pulses contain oligosaccharides which may cause intestinal disturbances such as
flatulence. This study was undertaken to investigate α-galactosidase-producing probiotics bacteria.
The enzymes and cultures can be added to foods in order to enhance the digestibility of
carbohydrates in the gastrointestinal tract. However since many of these bacteria are reported for
probiotic properties that support and induced health benefits to the consumer. The study provides
data on the stability of α-galactosidase, which could potentially be added to food matrices
containing stachyose or raffinose such as beans, soya and other pulses and could be an alternative
or remedies of oligosaccharides intolerance.
This document discusses food safety and spoilage of fermented foods. It begins by defining food safety and the properties of fermented foods, noting they are generally safer than unfermented foods due to inhibition of pathogenic bacteria and toxins. However, some hazards like E. coli and viruses may survive fermentation. It emphasizes using good practices like hygiene and a Hazard Analysis Critical Control Point (HACCP) system to ensure safety. The document then discusses causes of spoilage in fermented products like beer, wine, vegetables and cheeses by various microorganisms. It concludes by outlining advances in fermentation including engineering microorganisms and metabolic pathways.
This document discusses several non-lactic acid bacteria used as starter cultures in food fermentation and their properties, including Bifidobacterium, Propionibacterium, Brevibacterium, and Acetobacter. Bifidobacterium produces acetic and lactic acid and can grow from 25-45°C, with an optimal pH of 6.5-7.0. Propionibacterium is essential for flavor development in Swiss cheeses and grows internally from 25-32°C with an optimal pH of 6.5-7.0. Brevibacterium linens produces enzymes and compounds responsible for flavor and color in surface-ripened cheeses, growing aerobically from 20-
Describe el metabolismo de los carbohidratos en bacterias bacterias ácido lácticas , en especifico de las Lactobacillus, danfo a conocer los distintos tipos de metabolismo, cómo el homofermentativo, el ácido mixto y el heterofermentativo obligado, se muestran en un diagrama los distintos metabolitos para cada tipo de fermentación, además de las enzimas asociadas a cada uno de los procesos
Isolation of lactobacilli sp. from various nondiary substratesDr. sreeremya S
Lactobacilli are the major type of lactic acid bacteria (LAB) which have been shown to act as a preservative as well as a probiotic agent [1]. Probiotics means, it provides beneficial effects of microorganisms on human health. Probiotics are products availed as dietary supplements to copious the growth and health of the humans and animals [2].
ASSESSMENT OF LACTOBACILLUS SPP. POPULATION FROM TUBA INOCULATED IN DIFFERENT BEVERAGES
Tuba samples were mainly taken from coconut trees. These samples were placed directly in five sterile plastic bottles, having 100 ml of tubâ in each bottle, as soon as the harvester of tuba reaches the ground [3, 4]. It was taken from the coconut trees mainly in the afternoon and was immediately brought to the laboratory for Lactobacillus isolation, purification, inoculation, and population counting in four beverages [5].
This document summarizes information about the bacteria Lactobacillus acidophilus:
1) L. acidophilus was discovered in the early 1900s by Austrian pediatrician Ernst Moro when he isolated it from the stomach of children.
2) It is a homofermentative, rod-shaped bacterium that is typically isolated using selective media and microaerophilic conditions. Common isolation methods include using tomato juice medium or Raka ray no. 3 medium.
3) L. acidophilus provides several benefits including aiding digestion, exhibiting strong antimicrobial properties, demonstrating anticancer and anti-oxidative effects, and degrading organic acids. Maintaining viable cultures requires methods like
Fermented foods are foods that have been subjected to microorganisms or enzymes to cause desirable changes. Microorganisms involved in fermentation help preserve foods, extend shelf life, and improve flavor, aroma, nutrition, and digestibility. Some of the earliest fermented foods include wine, soy sauce, bread, cheese, and pickled vegetables. Lactic acid bacteria, including Lactobacillus, Lactococcus, and Leuconostoc species, are important microorganisms used as starters in food fermentation processes. Fermentation converts sugars to lactic acid, lowering pH and preventing spoilage while developing new flavors.
The document discusses yeasts that are used as starter cultures in fermented foods. It provides background on the early history of fermentation and then discusses the properties of yeast cells. It outlines the different types of metabolisms in yeasts and how they are classified. The document then examines the various roles yeasts play in fermenting different types of foods like dairy products, meats, cereals, beverages and more. It provides examples of common yeast species used in fermenting these different food categories.
Præsentation indhold er lavet af et Biotec virksomhed, og Digitypes har stået for design opsætningen. Designet er lavet så firmaet på egen hånd via Powerpoint kan ændre i designet
This document is a student project on microbes in human welfare submitted by Alok Kumar Bind. It includes an introduction to microbes, their role in food production including cheese, wine and curd making. It also discusses the use of microbes in water treatment processes like primary, secondary and tertiary treatment. Finally, it covers the use of microbes in energy production such as algae fuel, cellulosic fuel and biogas. The project was submitted to fulfill biology practical examination requirements.
Term paper on microbial ecology of fermented foods and beveragesChala Dandessa
This document provides information on the microbial ecology of various traditionally fermented foods and beverages in 3 sections. Section 1 describes various fermented products from Ethiopia including yogurt, cheese, injera (fermented flatbread), wakalim (fermented meat), and beverages like tella, shamita and borde. Section 2 defines probiotics, prebiotics, synbiotics and single cell protein and explains Hazard Analysis Critical Control Points (HACCP). Section 3 provides a summary and section 4 lists references.
This document discusses food microbiology and bacteria important in food science. It covers topics like the definition and importance of food microbiology, general characteristics of bacteria, morphological and physiological traits of bacteria, and important groups of bacteria in food including lactic acid bacteria, acetic acid bacteria, and thermophilic bacteria. It also discusses the roles bacteria play in food processing, preservation, and spoilage as well as their use in producing metabolites like amino acids, organic acids, enzymes, and vitamins.
presented by HAFIZ M WASEEM
university of education LAHORE Pakistan
i am from mailsi vehari and studied in lahore
bsc in science college multan
msc from lahore
Similar to Lactic acid bacteria in food industry (20)
This document discusses molds used as starter cultures in foods and their properties. It provides information on the structure and growth conditions of molds. Major molds used in food production include Penicillium camemberti and P. roqueforti for cheesemaking, and P. nalgiovense for fermented meat sausages. Aspergillus oryzae and A. niger are also used for producing enzymes in foods. Molds are useful in the food industry due to their lytic enzymes and ability to produce flavors and textures in fermented products like cheese and cured meats.
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2. Introduction
– Lactic acid bacteria (LAB) are commonly detected in various habitats
such as foodstuffs, gut and mucous membranes of humans and animals,
and in many environmental niches. In the fermentations of yogurt,
cheese, salami, sourdough bread, and wines, LAB are the key organisms
providing both desired sensory changes and increased shelf life and
product safety (Björkroth & Koort, 2016). The ability of lactic bacteria to
be involved in different fermentation processes results from the
heterogeneity of the groups. In the following we will discuss about the
group of lactic acid bacteria used in food technology as well as their
properties.
3. Characteristics of lactic acid bacteria
(LAB)
– Typical LAB are Gram-positive, non-spore-forming rods or cocci producing lactic
acid as the major end product of fermentation of glucose. There have been
frequent changes in the taxonomic classification of LAB, but most classification
schemes agree that genera in the order Lactobacillales, which includes
Lactobacillus, Leuconostoc, Pediococcus, Lactococcus, and Streptococcus, in
addition to Carnobacterium, Enterococcus, Oenococcus, Tetragenococcus,
Vagococcus, and Weissella, are the genera of LAB (O'Bryan, Crandall, Ricke, &
Ndahetuye, 2015).
4. Genera of Lactic Acid Bacteria Important to
Food Fermentations adapted from (Erten, 2016)
Old Classification New Classification
Lactobacillus Lactobacillus
Carnobacterium
Leuconostoc Leuconostoc
Oenococcus
Streptococcus Streptococcus thermophilus
Lactococcus
Enterococcus
Vagococcus
Pediococcus Pediococcus
Aerococcus
Tetragenococcus
5. Characteristics of lactic acid bacteria
(LAB)
– Based on sugar fermentation patterns, two broad metabolic categories of LAB
exist: homofermentative and heterofermentative. The main products of
fermentation include organic acids, alcohol, and carbon dioxide, although LAB
may also produce aromatic molecules, vitamins, or bioactive peptides (O'Bryan
et al., 2015).
– Those that produce lactic acid as the major or sole product of glucose
fermentation are designated homofermentative. Those that produce equal
amounts of lactic acid, ethanol and CO2 are termed heterofermentative. The
homolactics are able to extract about twice as much energy from a given
quantity of glucose as the heterolactics (Erten, 2016).
6. Characteristics of lactic acid bacteria
(LAB)
– All members of Pediococcus, Lactococcus, Streptococcus, Vagococcus, along
with some lactobacilli are homofermenters. Carnobacterium, Oenococcus,
Enterococcus, Weissella and Leuconostoc and some Lactobacilli are
heterofermenters. The heterolactics are more important than the homolactics
in producing flavour and aroma components such as acetylaldehyde and
diacetyl (Erten, 2016).
7. Characteristics of lactic acid bacteria
(LAB)
– LAB have a very limited capacity to synthesize amino acids using inorganic
nitrogen sources and are therefore dependent on preformed amino acids being
present in the growth medium; this requirement for amino acids differs among
species and strains within species, with some strains requiring as many as 15
preformed amino acids. Because free amino acids are not typically present in
sufficient concentrations in the environment, the LAB require a proteolytic
system capable of hydrolyzing peptides and proteins to obtain their essential
amino acids. The proteolytic activity of LAB contributes additionally to the
development of the flavor, aroma, and texture of fermented products; for
instance, many varieties of cheeses, such as Swiss and Cheddar, rely on
proteolysis for their desirable flavor notes (O'Bryan et al., 2015).
9. Lactococcus
– The genus Lactococcus comprises seven species, Lactococcus lactis (including
the subspecies cremoris, lactis, and hordniae), L. garvieae, L. piscium, L.
plantarum, and L. raffinolactis, L. chungangensis, and the quite recently
characterized L. fujiensis.
– Species identification is based on physiological, chemotaxonomic, and
molecular biological criteria. Morphologically, lactococci are all non-motile,
gram-positive cocci of 0.5–1.5 µm in size, forming short chains. They are
mesophilic, facultative anaerobes, with an optimum growth of Temperature
near 30°C, ferment hexoses homofermentatively producing l (+) lactic acid, and
have complex growth requirements (Erten, 2016; LAHTINEN, OUWEHAND,
SALMINEN, & WRIGHT, 2011).
10. Lactococcus
– Some physiological characteristics differentiating the species are listed in Table.
In practice, it is often difficult to distinguish between L. lactis and L. garvieae.
Pyrrolidonylarylamidase activity has been considered specific for the latter
species, but L. lactis strains having this activity are also frequently met. Thus
molecular biological identification is usually necessary to obtain a reliable
identification (LAHTINEN et al., 2011).
– One species in particular L. lactis, is among the most important of all lactic acid
bacteria (and perhaps one of the most important organisms involved in food
fermentations, period). L. lactis is used as a starter culture for most of the hard
cheeses and many of the cultured dairy products produced around the world
(Erten, 2016).
12. Lactococcus
– Thus far Lactococcus lactis ssp. lactis and cremoris are the only lactococci used
as dairy starters. The potential of a few strains of L. lactis ssp. hordniae, L.
raffinolactis, L. garvieae, L. piscium, and L. plantarum in dairy applications has
been assessed. The strains were characterized for phage resistance, lactose
fermentation, and growth in milk supplemented with glucose and casein
hydrolysate.
– The most promising were the L. raffinolactis strains; however, even they lacked
proteinase activity, and the activity was not expressed even after the
introduction of the proteinase-associated plasmid from L. lactis ssp. cremoris. L.
garvieae has been proposed for starter culture preparations, due to its
prevalence in artisanal Italian cheeses and its potential contribution to the
typical sensory characteristics of traditional cheeses (LAHTINEN et al., 2011).
13. Lactobacillus
– Lactobacillus is the largest genus within the group of lactic acid bacteria. To date
(July 2010), it contains 168 species, some of which are used in the manufacture of
fermented dairy, sourdough, meat, and vegetable foods, or used as probiotics.
Lactobacilli are Gram-positive, catalase-negative, non-spore forming, rod-shaped
bacteria that produce lactic acid as the major end product of fermentation (Calasso
& Gobbetti, 2011; De Angelis & Gobbetti, 2016)
– Ecologically, lactobacilli occupy a wide range of habitats. They are frequently found
in dairy and meat environments, in juice and fermented beverages, and in grains
and cereal products. Their presence in the animal and human gastrointestinal tract
(as well as in the stomach, mouth) has led to the suggestion that these bacteria
have broad “probiotic activity”. In foods, they are involved not only in much
important fermentation, but are also frequently implicated in spoilage of fermented
and non-fermented foods (Erten, 2016).
14. Lactobacillus
– Although most species are mesophilic, the genus also contains species that are
psychrotrophic, thermoduric, or thermophilic. Temperature optima varies
widely, from 30°C to 45°C but they can grow over a range of 5– 53 C. They also
are aciduric with an optimum growth pH of 5.5–5.8; in general, they can grow at
a pH <5 (C. A. Batt, 2014; Erten, 2016). Acid-tolerance is a common trait of
lactobacilli (many strains actually prefer an acidic environment), and some also
are ethanol-tolerant or bile-tolerant. Some species show high tolerance to salt,
osmotic pressure, and low water activity. Most species are aero tolerant,
whereas others require more strict anaerobic conditions (Erten, 2016).
16. Lactobacillus
– Given the diversity of metabolic properties exhibited by members of the
Lactobacillus genus, they are found in a number of fermented food products. In
these products, the lactobacilli contribute to their preservation, nutrition
availability, and flavor. Lactobacilli are added as deliberate starters or take part
in the fermentation as a result of their being natural contaminants of the
starting substrates. A number of dairy products are produced using
Lactobacillus either alone or in combination with other lactic acid bacteria.
Acidophilus milk is an example of a fermented dairy product and L. acidophilus
is the organism used to produce it. Lactobacillus bulgaricus in combination with
Streptococcus thermophilus is used to produce yogurt, and a balance between
these two starters can affect product quality. Vegetables are fermented with
lactobacilli to produce products, including pickles, olives, and sauerkraut.
17. Lactobacillus
– Lactobacillus spp. play an essential role in breadmaking and a number of unique
strains have been identified in products, most notably sourdough bread. Typical
species of lactobacilli identified in sourdough bread include L. acidophilus,
Lactobacillus farciminis, L. delbrueckii subsp. delbrueckii, L. casei, L. plantarum,
Lactobacillus rhamnosus, L. brevis, Lactobacillus sanfrancisco, and L. fermentum.
The exact composition of most sourdough breads is not known and attempts to
blend starters to mimic a particular product are sometimes less than satisfactory.
Traditional sourdough fermentations are carried out by ‘backslopping,’ a process
in which a small fraction of a prior batch is used to start the next batch. The
indigenous lactobacilli are able to overcome other contaminating microflora
largely by thriving under the fermentation conditions (C. A. Batt, 2014).
18. Lactobacillus
– During the fermentation process, lactic acid builds up levels approaching 1%
and a small amount of acetic acid is also produced. The number of lactic acid
bacteria can reach 107 cfu g-1
– Another property of lactobacilli that has become more appreciated is their
ability to produce bacteriocins. The bacteriocins produced by lactobacilli are
presented in the next table. Bacteriocins probably evolved to provide the
producing organism with a selective advantage in a complex microbial niche.
Incorporation of Lactobacillus spp. as starters or the inclusion of a purified or
semi purified bacteriocin preparation as an ingredient in a food product may
provide a margin of safety in preventing pathogen growth (C. A. Batt, 2014).
19. Streptococcus
– The genus Streptococcus consists of Gram-positive, spherical ovoid, or
coccobacillary cells, with a diameter less than 2 mm, that form chains or pairs.
Cells in older cultures may appear Gram variable, and some strains are
pleomorphic. Streptococcus spp. are nonsporing and nonmotile. Streptococci
are catalase negative, with the exception of the recently described species
Streptococcus didelphis, which, on initial isolation on blood agar, gives vigorous
catalase activity that is lost after several passages. They ferment carbohydrates
to produce mainly lactic acid, but no gas, and have complex nutritional
requirements. Under glucoselimiting conditions, formate, acetate, and ethanol
are also produced. Most are facultatively anaerobic or aerotolerant anaerobes;
some are capnophilic (CO2-requiring) (Gobbetti & Calasso, 2014).
20. Streptococcus
– Except for S. thermophilus, streptococci sensu stricto are not currently used in
food fermentation. The naturally occurring S. gallolyticus subsp. macedonicus is
another promising multifunctional Streptococcus starter culture. Some
pathogens, however, are introduced into humans and animals by foods
(Gobbetti & Calasso, 2014).
– Streptococcus thermophilus belongs to the thermophilic group of lactic acid
bacteria. It is traditionally used in association with one or several Lactobacillus
species as a starter culture in the production of yogurt and the manufacture of
Swiss and Italian cheeses such as Emmentaler and Mozzarella.
21. Streptococcus
– Streptococcus thermophilus is a homofermentative bacterium, fermenting lactose
via the Embden–Meyerhof pathway (EMP) to L(+) lactate, Gram-positive spherical
to ovoid nonmotile coccus, 0.7–0.9 mm in diameter, occurring in pairs and chains,
some of which can be very long. The bacterium has an optimum growth
temperature of 40–45 °C, a minimum of 20–25 °C, and a maximum near 47–50
°C. Streptococcus thermophilus does not hydrolyze arginine. It ferments a limited
number of sugars including lactose, fructose, sucrose, and glucose. Streptococcus
thermophilus does not ferment galactose during lactose metabolism. It is also
characterized by being relatively sensitive to antibiotics and sanitizers and having
low proteolytic activity. It is unique among the streptococci in having no group-
specific antigen. Strains of S. thermophilus that produce bacteriocins are rare, and
those that have been isolated have not been well characterized (Harnett, Davey,
Patrick, Caddick, & Pearce, 2011).
22. Pediococcus
– Pediococcal colonies vary in size (1.0–2.5 mm in diameter), and they are smooth,
round, and greyish white. All species grow at 30 °C, but the optimum temperature
range is 25–40 °C. Pediococcus pentosaceus has a lower optimum temperature for
growth (28–32 °C) than P. acidilactici (40 °C), but the latter grows at 50 °C. The
optimum pH for growth is 6.0–6.5. Half of the species grow at pH 4.2, and most of
them (except P. damnosus) grow at pH 7.0. Most Pediococcus species (except for P.
damnosus) can grow in the presence of 4.0 and 6.5% NaCl but not in the presence of
10% NaCl.
– Lactic acid production by P. pentosaceus, in a bacteriological medium at 27 °C, is
inhibited 36.0–51.0% by concentrations of NaCl from 3.0 to 3.9% (w/v), respectively.
Some strains of P. acidilactici and P. pentosaceus have proteolytic enzymes, such as
protease, di-peptidase, dipeptidyl aminopeptidase, and amino-peptidase. Pediococcus
pentosaceus shows strong leucine and valine arylamidase activities (Carl A. Batt &
Tortorello, 2014).
23. Pediococcus
– Pediococci are gram-positive, nonmotile oxidase-negative, and catalase-negative
organisms occurring as spherical cells uniform in size that form tetrads via alternate
division in two perpendicular directions. Pediococci are facultatively aerobic
homofermenters that produce lactic acid as the major end product of glucose
fermentation by the EMP to dl-lactic acid except for strains of Pediococcus claussenii,
which convert glucose to l (+)-lactic acid. Fructose, mannose, and cellobiose are
fermented by all species. Most species are also able to ferment galactose and
maltose, although it has been reported that some strains of Pediococcus damnosus,
Pediococcus Pparvulus, and . claussenii lack this ability. Sucrose is also fermented by
all species except Pediococcus inopinatus, P. parvulus, Pediococcus pentosaceus, and
P. claussenii. In contrast, rhamnose, melibiose, melezitose, raffinose, inulin, and α-
methyl glucoside D are not fermented by most pediococci. Like many other LAB
pediococci also produce bacteriocins (i.e., pediocins).
24. Pediococcus
– Producer strains of pediocins have mainly been found in the phylogenetically and
biochemically related species Pediococcus acidilactici and P. pentosaceus and, more
recently, also in P. damnosus (LAHTINEN et al., 2011).
– The pediococci are used in the commercial fermentation of meats, vegetables, and
sour wheat flour with no added sugar. Lactose-positive pediococci may replace
Streptococcus thermophilus in Italian cheese starter blends to combat S.
thermophilus bacteriophage problems in mozzarella cheese plants. Pediococcus
acidilactici and P. pentosaceus are used in the fermentation of meats. Manganese
enhances the fermentation of meats at a suboptimal incubation temperature for
the culture. Pediococci were inhibited by KCl, as a salt substitute. The use of mix
starter cultures could be a problem as some strains of pediococci may inhibit the
growth of other strains of pediococci, L. plantarum, and Leuconostoc mesenteroides
(Carl A. Batt & Tortorello, 2014).
25. Pediococcus
– The pediococci may be useful as biopreservatives to control the growth of
Salmonella typhimurium and Pseudomonas sp. (in pasteurized liquid whole
eggs and cooked mechanically deboned poultry meat), Staphylococcus aureus
(cooked mechanically deboned poultry meat), and Listeria (milk). Pediococci
also increase the shelf life of refrigerated mechanically deboned poultry meat,
ground beef, and ground poultry breast. There are conflicting reports as to the
inhibition of Clostridium botulinum by pediococcal bacteriocin. Pediocin may be
effective in controlling Listeria in milk and during the fermentation of turkey
summer sausage. Both P. acidilactici and P. pentosaceus may control the growth
of Yersinia enterocolitica serotype 0:3 and 0:8 in fermenting meat (Carl A. Batt
& Tortorello, 2014).
26. Tetragenococcus
– Phylogenetically, the genus Tetragenococcus is a recognized member of the
family Enterococcaceae within the order Lactobacillales. Morphologically,
tetragenococci cannot be readily distinguished from members of the genus
Pediocccus. Both genera also share a facultative aerobic homofermentative
metabolism, the ability to ferment a relatively wide range of sugars.
– T. halophilus and T. muriaticus have primarily been associated with habitats rich
in salt and protein. Both salt-tolerant species are known to play an important
role in halophilic fermentation processes such as the production of soy sauce,
soy paste, brined anchovies, fish sauce, Japanese fermented puffer fish ovaries,
Indonesian “terasi” shrimp paste, and fermented mustard.
27. Tetragenococcus
– . However, T. halophilus also constitutes the dominant microbiota in
concentrated sugar thick juice, a sugar-rich intermediate in the production of
beet sugar. In fact, strains of this species have been associated with thick juice
degradation, a process characterized by a pH shift from pH 9 to 5–6 and by an
increase in reducing sugar content resulting in economic losses. Tetragenococci
can be readily distinguished from pediococci mainly by their high salt tolerance
(depending on the species, from 6.5% to 25% NaCl [w/v]) and ability to grow at
high pH values up to 9.0 but not at pH 5.0 (LAHTINEN et al., 2011).
28. Leuconostoc
– Leuconostocs are mesophilic, Gram-positive, catalase-negative, nonmotile,
aerotolerant, obligately heterofermentative cocci, often ellipsoidal. They usually
occur in pairs and chains (Holland & Liu, 2011; LAHTINEN et al., 2011). When
grown on a solid medium, cells are elongated and can be mistaken for rods.
True cellular capsules are not formed, but many leuconostocs produce
extracellular dextran that forms an electron-dense coat on the cell surface.
Growth may occur at pH 4.5, leuconostocs prefer an initial medium pH of 6.5.
The optimal growth temperature is between 20°C and 30°C (LAHTINEN et al.,
2011). Leuconostoc spp. are widespread in the environment, and have been
isolated from plant matter, human clinical sources, and foods such as chill-
stored and fermented meats, fermented vegetables (e.g., sauerkraut, kimchi),
and fermented dairy products (e.g., cheese, kefir, yogurt).
29. Leuconostoc
– The exact species of Leuconostoc occurring in dairy starter cultures are not
always defined, but generally there are only two: Ln. lactis and Ln.
mesenteroides (subsp. mesenteroides, subsp. cremoris, and subsp. dextranicum).
Leuconostoc mesenteroides subsp. cremoris is the subspecies most frequently
isolated from mesophilic mixed-strain dairy starter cultures and from fermented
dairy products. In addition, Ln. citreum has been reported as an isolate from
cheese. A number of bacteriophages that attack some dairy leuconostocs have
been isolated. However, most leuconostocs are insensitive to bacteriophages.
There are no reports of bacteriophage attack in dairy fermentations associated
with leuconostocs. Dairy leuconostocs are known to produce antimicrobial
compounds against pathogenic and spoilage microorganisms.
30. Leuconostoc
– However, the inhibitory activity is generally attributed to the action of organic
acids such as acetic acid, CO2, and H2O2 that they produce. In general,
bacteriocins produced by leuconostocs may not necessarily be active against
lactic acid bacteria, but are active against Listeria monocytogenes, a major food
pathogen (Holland & Liu, 2011).
32. Weissella
– Weissellas are gram-positive, nonmotile, and asporogenous short rods with
rounded tapered ends, or ovoid. They occur in pairs or in short chains, and
there is tendency toward pleomorphism in some of the species. They are
catalase negative, facultatively anaerobic chemo-organotrophs, and were
originally considered not to contain cytochromes. Weissellas ferment glucose
heterofermentatively. Carbohydrates are fermented via the hexose
monophosphate and phosphoketolase pathways. End products of glucose
fermentation are CO2, ethanol, and/or acetate. Depending on the species, the
configuration of the lactic acid produced is either dl- or d (–). Weissellas have
complex nutritional requirements as amino acids, peptides, fermentable
carbohydrates, fatty acids, nucleic acids, and vitamins are generally required for
growth. Biotin, nicotine, thiamine, and panthotenic acid or its derivatives are
required.
33. Weissella
– Arginine is not hydrolyzed by all species. Growth occurs at 15°C; some species
grow at 42–45°C.
– Members of W. cibaria, W. confusa, and W. koreensis have been detected in
fermented foods of vegetable origin, whereas W. confusa has been associated
with Greek salami, Mexican pozol, and Malaysian chili bo. W. cibaria and W.
confusa have also been associated with various types of sourdoughs. W.
ghanensis and W. fabaria were detected in traditional heap fermentations of
Ghanaian cocoa bean. W. beninensis originates from submerged fermenting
cassava (LAHTINEN et al., 2011).
34. Oenococcus
– The name Oenococcus refers to ‘a little round berry from wine’ and only one
species is found in wine; Oenococcus oeni. This species is well adapted to the
harsh wine environment: low nutrients, high acidity, and high ethanol
concentration. When viewed under a microscope, the cells are ellipsoidal to
spherical in shape, usually present in pairs, but they will form chains in the
presence of ethanol. They form small colonies on solid media (agar), and
growth is slow, usually 7–10 days at 22 C. O. oeni will grow better, and even be
stimulated, in the presence of lower oxygen concentrations (micro-aerophilic)
(Carl A. Batt & Tortorello, 2014)
35. Oenococcus
– Next to the type species O. oeni, the genus Oenococcus also comprises the
nonacidophilic and non-malolactic-fermenting species Oenococcus kitaharae,
which was proposed to accommodate isolates from a composting distilled
shochu residue in Japan (LAHTINEN et al., 2011).
36. References
– Batt, C. A. (2014). LACTOBACILLUS | Introduction. 409-411.
doi:10.1016/b978-0-12-384730-0.00176-2
– Batt, C. A., & Tortorello, M. L. (2014). Encyclopedia of food
microbiology Encyclopedia of food microbiology.
– Björkroth, J., & Koort, J. (2016). Lactic Acid Bacteria: Taxonomy
and Biodiversity Reference Module in Food Science: Elsevier.
– Calasso, M., & Gobbetti, M. (2011). Lactic Acid Bacteria |
Lactobacillus spp.: Other Species A2 - Fuquay, John W
Encyclopedia of Dairy Sciences (Second Edition) (pp. 125-131).
San Diego: Academic Press.
– De Angelis, M., & Gobbetti, M. (2016). Lactobacillus SPP.:
General Characteristics Reference Module in Food Science:
Elsevier.
– Erten, H. (2016). Course note : physiology and biotechnology of
lactic acid bacteria. University of Çukurova, Faculty of
Agriculture, Department of food engineering, Adana.
– Gobbetti, M., & Calasso, M. (2014). STREPTOCOCCUS |
Introduction. 535-553. doi:10.1016/b978-0-12-384730-0.00324-
4
– Harnett, J., Davey, G., Patrick, A., Caddick, C., & Pearce, L.
(2011). Lactic Acid Bacteria | Streptococcus thermophilus A2 -
Fuquay, John W Encyclopedia of Dairy Sciences (Second Edition)
(pp. 143-148). San Diego: Academic Press.
– Holland, R., & Liu, S. Q. (2011). Lactic Acid Bacteria |
Leuconostoc spp. A2 - Fuquay, John W Encyclopedia of Dairy
Sciences (Second Edition) (pp. 138-142). San Diego: Academic
Press.
– LAHTINEN, S., OUWEHAND, A. C., SALMINEN, S., & WRIGHT, A.
V. (2011). Lactic acid bacteria : Microbiological and functional
aspects (pp. 779).
– O'Bryan, C. A., Crandall, P. G., Ricke, S. C., & Ndahetuye, J. B.
(2015). Lactic acid bacteria (LAB) as antimicrobials in food
products. 117-136. doi:10.1016/b978-1-78242-034-7.00006-2